Shigella phage SfIV

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5P0S6|U5P0S6_9CAUD Bactoprenol-linked glucose translocase OS=Shigella phage SfIV OX=1407493 GN=SfIV_27 PE=3 SV=1
MM1 pKa = 7.98AIHH4 pKa = 6.92ISASTEE10 pKa = 3.76YY11 pKa = 10.49LAVGVGIPAYY21 pKa = 10.35SCLDD25 pKa = 3.23APGSYY30 pKa = 9.89KK31 pKa = 10.44AGYY34 pKa = 8.97AICRR38 pKa = 11.84SADD41 pKa = 3.53FNSWEE46 pKa = 4.19YY47 pKa = 11.36VPDD50 pKa = 3.52HH51 pKa = 6.82RR52 pKa = 11.84GEE54 pKa = 4.11NVYY57 pKa = 9.24STEE60 pKa = 3.99TGEE63 pKa = 4.41SKK65 pKa = 10.78EE66 pKa = 3.74ITALGDD72 pKa = 3.52YY73 pKa = 10.31PEE75 pKa = 4.54NTTTIAPLTPYY86 pKa = 10.6DD87 pKa = 3.4KK88 pKa = 10.5WDD90 pKa = 3.49GEE92 pKa = 4.17KK93 pKa = 10.04WVTDD97 pKa = 3.46TEE99 pKa = 4.47AQHH102 pKa = 5.9SAAVGAAEE110 pKa = 4.28VQRR113 pKa = 11.84QSLVDD118 pKa = 3.28AAMASISLIQLKK130 pKa = 9.88LQAGRR135 pKa = 11.84KK136 pKa = 6.48LTQAEE141 pKa = 4.49TTRR144 pKa = 11.84LNAVLDD150 pKa = 4.24YY151 pKa = 10.71IDD153 pKa = 5.46AVTATDD159 pKa = 3.41TSTAPDD165 pKa = 4.27VIWPEE170 pKa = 3.88LPEE173 pKa = 4.01AA174 pKa = 4.64

Molecular weight: 18.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5P0U9|U5P0U9_9CAUD I-spanin (Rz) OS=Shigella phage SfIV OX=1407493 GN=SfIV_52 PE=4 SV=1
MM1 pKa = 7.77PGNSPHH7 pKa = 6.42YY8 pKa = 10.2GRR10 pKa = 11.84WPQHH14 pKa = 6.55DD15 pKa = 3.89FTSLKK20 pKa = 10.21KK21 pKa = 10.5LRR23 pKa = 11.84PQSVTSRR30 pKa = 11.84IQPGSDD36 pKa = 3.24VIVCAEE42 pKa = 4.04MDD44 pKa = 3.97EE45 pKa = 4.08QWGYY49 pKa = 11.45VGAKK53 pKa = 9.33SRR55 pKa = 11.84QRR57 pKa = 11.84WLFYY61 pKa = 10.56AYY63 pKa = 10.34DD64 pKa = 3.9SLRR67 pKa = 11.84KK68 pKa = 7.62TVVAHH73 pKa = 5.58VFGEE77 pKa = 4.23RR78 pKa = 11.84TMATLGRR85 pKa = 11.84LMSLLSPFDD94 pKa = 3.51VVIWMTDD101 pKa = 2.56GWPLYY106 pKa = 9.76EE107 pKa = 4.63SRR109 pKa = 11.84LKK111 pKa = 11.14GKK113 pKa = 9.61LHH115 pKa = 6.35VISKK119 pKa = 10.15RR120 pKa = 11.84YY121 pKa = 4.5TQRR124 pKa = 11.84IEE126 pKa = 3.79RR127 pKa = 11.84HH128 pKa = 5.1NLNLRR133 pKa = 11.84QHH135 pKa = 6.9LARR138 pKa = 11.84LGRR141 pKa = 11.84KK142 pKa = 8.76SLSFSKK148 pKa = 10.61SVEE151 pKa = 3.91LHH153 pKa = 6.59DD154 pKa = 5.11KK155 pKa = 11.16VIGHH159 pKa = 6.18YY160 pKa = 10.87LNIKK164 pKa = 9.58HH165 pKa = 5.41YY166 pKa = 9.45QQ167 pKa = 3.14

Molecular weight: 19.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12059

66

635

223.3

24.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.79 ± 0.387

1.327 ± 0.171

5.813 ± 0.25

5.987 ± 0.277

3.64 ± 0.305

6.908 ± 0.304

1.874 ± 0.171

5.498 ± 0.362

5.639 ± 0.377

9.047 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.158

3.881 ± 0.183

4.428 ± 0.217

3.715 ± 0.193

6.485 ± 0.358

6.808 ± 0.326

6.186 ± 0.303

6.609 ± 0.239

1.717 ± 0.158

3.052 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski