Fusarium oxysporum f. sp. raphani 54005
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22754 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X0C8Z9|X0C8Z9_FUSOX Uncharacterized protein OS=Fusarium oxysporum f. sp. raphani 54005 OX=1089458 GN=FOQG_07011 PE=3 SV=1
MM1 pKa = 7.75 PRR3 pKa = 11.84 TIDD6 pKa = 3.33 WGEE9 pKa = 3.38 GRR11 pKa = 11.84 YY12 pKa = 9.39 PKK14 pKa = 10.67 GNRR17 pKa = 11.84 CEE19 pKa = 3.93 YY20 pKa = 11.36 DD21 pKa = 3.03 NAKK24 pKa = 9.1 WKK26 pKa = 10.48 SGYY29 pKa = 10.4 GYY31 pKa = 9.85 WILWGTDD38 pKa = 2.91 RR39 pKa = 11.84 DD40 pKa = 3.72 NGGVYY45 pKa = 10.03 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 HH49 pKa = 5.3 WYY51 pKa = 10.38 CSISCRR57 pKa = 11.84 NMDD60 pKa = 5.89 AEE62 pKa = 4.65 CCDD65 pKa = 4.1 CNKK68 pKa = 10.36 EE69 pKa = 4.28 DD70 pKa = 6.14 DD71 pKa = 4.08 SSEE74 pKa = 4.36 DD75 pKa = 4.14 NSDD78 pKa = 5.45 DD79 pKa = 5.87 DD80 pKa = 6.69 DD81 pKa = 6.38 DD82 pKa = 5.79 DD83 pKa = 6.82 HH84 pKa = 9.41 DD85 pKa = 5.49 DD86 pKa = 4.96 DD87 pKa = 5.97 EE88 pKa = 5.43 EE89 pKa = 5.27 EE90 pKa = 4.51 EE91 pKa = 5.26 DD92 pKa = 5.37 DD93 pKa = 5.75 DD94 pKa = 6.55 SVDD97 pKa = 4.48 CVCQEE102 pKa = 3.84 MKK104 pKa = 10.99 AEE106 pKa = 4.5 DD107 pKa = 4.28 EE108 pKa = 4.34 EE109 pKa = 5.66 SDD111 pKa = 3.37
Molecular weight: 13.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.872
IPC_protein 3.884
Toseland 3.656
ProMoST 4.012
Dawson 3.872
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.567
Solomon 3.872
Lehninger 3.821
Nozaki 3.986
DTASelect 4.24
Thurlkill 3.706
EMBOSS 3.821
Sillero 3.999
Patrickios 1.914
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.91
Protein with the highest isoelectric point:
>tr|X0CPC0|X0CPC0_FUSOX R3H-assoc domain-containing protein OS=Fusarium oxysporum f. sp. raphani 54005 OX=1089458 GN=FOQG_05726 PE=4 SV=1
MM1 pKa = 6.94 TGGMIVRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 QATTGWGIGSQSAKK25 pKa = 7.28 TQPRR29 pKa = 11.84 RR30 pKa = 11.84 SRR32 pKa = 11.84 GPAGVSS38 pKa = 3.1
Molecular weight: 4.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.465
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.106
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.135
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19288
3466
22754
10087366
30
7623
443.3
49.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.258 ± 0.017
1.348 ± 0.008
5.802 ± 0.011
6.203 ± 0.019
3.781 ± 0.011
6.685 ± 0.017
2.426 ± 0.008
5.114 ± 0.013
4.993 ± 0.016
8.852 ± 0.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.285 ± 0.007
3.815 ± 0.009
5.929 ± 0.019
4.093 ± 0.014
5.927 ± 0.016
8.154 ± 0.02
5.977 ± 0.021
6.025 ± 0.013
1.526 ± 0.007
2.806 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here