Paenibacillus sp. KS1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5401 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B8UWS0|A0A1B8UWS0_9BACL NADH dehydrogenase OS=Paenibacillus sp. KS1 OX=1849249 GN=BBG47_04045 PE=4 SV=1
MM1 pKa = 7.64TFTQEE6 pKa = 4.97LLDD9 pKa = 3.7SSINIISQYY18 pKa = 9.47PVWPNITKK26 pKa = 10.29YY27 pKa = 11.25SNDD30 pKa = 2.97NGIFANITLEE40 pKa = 4.27YY41 pKa = 9.72IWDD44 pKa = 3.71STTTDD49 pKa = 3.1NQVRR53 pKa = 11.84AFAAFSGGVGSEE65 pKa = 4.01ASSVSLDD72 pKa = 3.25VSLTVFADD80 pKa = 3.54NAHH83 pKa = 5.88VARR86 pKa = 11.84LDD88 pKa = 3.65VYY90 pKa = 11.05DD91 pKa = 5.83LSGQNPAFNTTLTPPVLTDD110 pKa = 3.34GSMDD114 pKa = 3.93ANTGLVEE121 pKa = 3.92ILPYY125 pKa = 9.69NWQNIRR131 pKa = 11.84IYY133 pKa = 10.58NISSDD138 pKa = 3.94PLPAGGIYY146 pKa = 10.43GILPLRR152 pKa = 11.84SLSS155 pKa = 3.55

Molecular weight: 16.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B8UMK3|A0A1B8UMK3_9BACL PTS glucose transporter subunit IIA OS=Paenibacillus sp. KS1 OX=1849249 GN=BBG47_21470 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 8.7PTFKK6 pKa = 10.81PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5401

0

5401

1749430

26

4981

323.9

36.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.997 ± 0.035

0.871 ± 0.01

5.144 ± 0.025

6.797 ± 0.035

4.011 ± 0.022

7.077 ± 0.032

2.358 ± 0.018

6.905 ± 0.033

5.346 ± 0.036

9.771 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.066 ± 0.019

3.854 ± 0.022

3.874 ± 0.018

4.17 ± 0.024

5.052 ± 0.03

6.342 ± 0.027

5.391 ± 0.023

7.128 ± 0.025

1.298 ± 0.013

3.548 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski