Tsukamurella phage TIN3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Tinduovirus; Tsukamurella virus TIN3

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0N5S5|A0A0K0N5S5_9CAUD Uncharacterized protein OS=Tsukamurella phage TIN3 OX=1636546 GN=TIN3_51 PE=4 SV=1
MM1 pKa = 7.68IDD3 pKa = 3.76DD4 pKa = 4.05ANKK7 pKa = 10.2RR8 pKa = 11.84AEE10 pKa = 4.4LKK12 pKa = 10.57QKK14 pKa = 10.87ALDD17 pKa = 3.7ALAEE21 pKa = 4.06YY22 pKa = 10.2LVAGGAMEE30 pKa = 5.66ADD32 pKa = 3.86QQIGDD37 pKa = 4.07CMIALSLVGALQDD50 pKa = 3.63NEE52 pKa = 4.3QYY54 pKa = 10.87RR55 pKa = 11.84CTVLAGSLSAASGLAGYY72 pKa = 10.31AGTVFGTYY80 pKa = 8.93MLGGEE85 pKa = 4.46VV86 pKa = 3.27

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0N545|A0A0K0N545_9CAUD Putative tape measure protein OS=Tsukamurella phage TIN3 OX=1636546 GN=TIN3_18 PE=4 SV=1
MM1 pKa = 7.32RR2 pKa = 11.84QKK4 pKa = 9.18KK5 pKa = 6.1TSRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84VEE12 pKa = 4.03VQVEE16 pKa = 4.4ANSSPRR22 pKa = 11.84GGWDD26 pKa = 2.58ICIRR30 pKa = 11.84DD31 pKa = 3.91AAGHH35 pKa = 6.83LSDD38 pKa = 3.84TTCVSRR44 pKa = 11.84NNVAFKK50 pKa = 9.81ATQEE54 pKa = 3.93AARR57 pKa = 11.84VLGVPEE63 pKa = 3.92GRR65 pKa = 11.84IKK67 pKa = 10.5INRR70 pKa = 11.84VVYY73 pKa = 9.8VV74 pKa = 3.62

Molecular weight: 8.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

23674

42

2909

217.2

23.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.394 ± 0.578

1.115 ± 0.163

6.235 ± 0.258

6.877 ± 0.376

3.434 ± 0.092

8.017 ± 0.473

1.711 ± 0.175

5.276 ± 0.175

5.529 ± 0.216

7.379 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.162

4.376 ± 0.124

5.001 ± 0.289

3.637 ± 0.18

5.69 ± 0.339

6.163 ± 0.157

5.935 ± 0.26

6.932 ± 0.221

1.622 ± 0.11

2.986 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski