Pectobacterium phage DU_PP_V

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Mccorquodalevirinae; Hongcheonvirus; Pectobacterium virus DUPPV

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D2W6V0|A0A2D2W6V0_9CAUD DNA polymerase III subunit epsilon OS=Pectobacterium phage DU_PP_V OX=2041492 GN=P13BB106kb_p034 PE=4 SV=1
MM1 pKa = 7.68SNNKK5 pKa = 9.03KK6 pKa = 9.66QALEE10 pKa = 3.73LAKK13 pKa = 10.47RR14 pKa = 11.84IVDD17 pKa = 3.07LHH19 pKa = 5.91MRR21 pKa = 11.84GSCEE25 pKa = 3.6SDD27 pKa = 3.46SYY29 pKa = 11.71FEE31 pKa = 4.22NLLIEE36 pKa = 4.73DD37 pKa = 4.24CASLFIEE44 pKa = 5.41LDD46 pKa = 3.29DD47 pKa = 4.11WDD49 pKa = 5.64ANDD52 pKa = 4.61LLCEE56 pKa = 3.91AMEE59 pKa = 4.14ILSVGRR65 pKa = 11.84CVHH68 pKa = 6.11CWWVVSDD75 pKa = 5.3PGVDD79 pKa = 3.95DD80 pKa = 5.47NGEE83 pKa = 4.44CTCSDD88 pKa = 3.63CVEE91 pKa = 4.17EE92 pKa = 6.02LEE94 pKa = 5.72DD95 pKa = 3.7EE96 pKa = 4.4

Molecular weight: 10.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D2W753|A0A2D2W753_9CAUD Major capsid protein OS=Pectobacterium phage DU_PP_V OX=2041492 GN=P13BB106kb_p060 PE=4 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.03SKK5 pKa = 8.69NTKK8 pKa = 8.48TVVVKK13 pKa = 9.63DD14 pKa = 3.71TSKK17 pKa = 10.01TEE19 pKa = 3.73ANRR22 pKa = 11.84ARR24 pKa = 11.84RR25 pKa = 11.84LARR28 pKa = 11.84HH29 pKa = 6.24LKK31 pKa = 9.86AHH33 pKa = 6.77PNDD36 pKa = 3.59AQTAKK41 pKa = 10.22AVKK44 pKa = 10.27SFTPRR49 pKa = 11.84AKK51 pKa = 10.18PQVKK55 pKa = 10.22GSTKK59 pKa = 10.12SRR61 pKa = 11.84AKK63 pKa = 10.17ARR65 pKa = 11.84LVAFVAGYY73 pKa = 7.59GHH75 pKa = 7.12KK76 pKa = 10.17SVPVVTGTYY85 pKa = 10.09LDD87 pKa = 3.63NEE89 pKa = 4.92FYY91 pKa = 10.46PKK93 pKa = 9.78GTMAPKK99 pKa = 10.38DD100 pKa = 3.73YY101 pKa = 9.63TRR103 pKa = 11.84QMSSRR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84EE111 pKa = 3.61LSKK114 pKa = 10.94FIMEE118 pKa = 3.9QRR120 pKa = 11.84ATFGSVKK127 pKa = 10.16PNLFGKK133 pKa = 10.45EE134 pKa = 3.72YY135 pKa = 10.44DD136 pKa = 3.94AEE138 pKa = 4.14DD139 pKa = 3.18ARR141 pKa = 11.84AICFHH146 pKa = 7.02LGIKK150 pKa = 10.15RR151 pKa = 11.84SSSKK155 pKa = 10.08PRR157 pKa = 11.84RR158 pKa = 11.84KK159 pKa = 10.1SKK161 pKa = 10.94

Molecular weight: 18.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

127

0

127

27596

51

1227

217.3

24.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.182 ± 0.339

1.036 ± 0.088

5.914 ± 0.149

6.987 ± 0.222

4.008 ± 0.158

6.262 ± 0.175

1.696 ± 0.128

6.534 ± 0.18

6.385 ± 0.224

8.929 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.109

5.128 ± 0.154

3.551 ± 0.167

3.501 ± 0.199

4.58 ± 0.128

7.425 ± 0.206

6.302 ± 0.226

6.9 ± 0.162

1.192 ± 0.077

4.106 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski