Aerococcus phage vB_AviM_AVP
Average proteome isoelectric point is 5.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 165 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S7SGJ1|A0A3S7SGJ1_9CAUD Uncharacterized protein OS=Aerococcus phage vB_AviM_AVP OX=2495576 GN=AVP_148 PE=4 SV=1
MM1 pKa = 7.73 ANLIEE6 pKa = 5.2 LIYY9 pKa = 10.48 EE10 pKa = 3.95 NTMNNGDD17 pKa = 4.49 DD18 pKa = 6.24 LMTFWNVMSEE28 pKa = 4.22 LEE30 pKa = 4.14 EE31 pKa = 4.13 TTISVDD37 pKa = 3.52 YY38 pKa = 11.34 GDD40 pKa = 4.52 DD41 pKa = 3.68 TQVPLLKK48 pKa = 10.25 ADD50 pKa = 3.63 NEE52 pKa = 4.44 AIEE55 pKa = 4.01 LHH57 pKa = 6.19 EE58 pKa = 4.68 FFDD61 pKa = 4.34 RR62 pKa = 11.84 EE63 pKa = 4.02 APYY66 pKa = 11.36 GEE68 pKa = 4.37 VQHH71 pKa = 6.97 LDD73 pKa = 3.48 QEE75 pKa = 4.52 EE76 pKa = 4.34 LEE78 pKa = 4.38 EE79 pKa = 4.55 LSEE82 pKa = 5.28 QGFEE86 pKa = 4.18 WEE88 pKa = 5.1 HH89 pKa = 6.07 LASDD93 pKa = 3.45 NTFNYY98 pKa = 10.42 SSFIEE103 pKa = 3.87 RR104 pKa = 11.84 DD105 pKa = 3.24 FNFNVFEE112 pKa = 4.51 SNIGDD117 pKa = 3.54 ILVTFNISTGLDD129 pKa = 3.1 PRR131 pKa = 11.84 GRR133 pKa = 11.84 YY134 pKa = 7.54 STGFAILFDD143 pKa = 4.36 SYY145 pKa = 11.59 DD146 pKa = 3.45 AYY148 pKa = 10.32 IEE150 pKa = 4.05 FMNEE154 pKa = 2.83 QFTFFNIEE162 pKa = 3.42 LRR164 pKa = 11.84 YY165 pKa = 8.38 TDD167 pKa = 3.53 EE168 pKa = 4.59 EE169 pKa = 4.58 GDD171 pKa = 3.35 EE172 pKa = 3.87 RR173 pKa = 11.84 RR174 pKa = 11.84 EE175 pKa = 3.84 YY176 pKa = 11.26 FEE178 pKa = 5.18 VNGSLFSHH186 pKa = 7.2 EE187 pKa = 4.13 IEE189 pKa = 5.06 LYY191 pKa = 10.93 CKK193 pKa = 10.76 DD194 pKa = 4.08 EE195 pKa = 5.52 DD196 pKa = 3.48 IQEE199 pKa = 4.32 YY200 pKa = 10.46 IEE202 pKa = 4.37 TYY204 pKa = 10.4 EE205 pKa = 4.71 LDD207 pKa = 3.44 VDD209 pKa = 4.78 SLLEE213 pKa = 4.04 QIKK216 pKa = 9.82 EE217 pKa = 3.99 LPLYY221 pKa = 10.59 EE222 pKa = 5.73 DD223 pKa = 4.53 IEE225 pKa = 4.86 DD226 pKa = 3.99 LEE228 pKa = 4.48 LWW230 pKa = 3.77
Molecular weight: 27.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.681
IPC_protein 3.656
Toseland 3.465
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.516
Rodwell 3.49
Grimsley 3.376
Solomon 3.617
Lehninger 3.567
Nozaki 3.732
DTASelect 3.884
Thurlkill 3.503
EMBOSS 3.528
Sillero 3.77
Patrickios 0.693
IPC_peptide 3.617
IPC2_peptide 3.757
IPC2.peptide.svr19 3.71
Protein with the highest isoelectric point:
>tr|A0A3S7SGS6|A0A3S7SGS6_9CAUD Uncharacterized protein OS=Aerococcus phage vB_AviM_AVP OX=2495576 GN=AVP_155 PE=4 SV=1
MM1 pKa = 7.57 GIVLGAFMGWIVGTLIVRR19 pKa = 11.84 TFIEE23 pKa = 4.24 IPEE26 pKa = 4.29 GRR28 pKa = 11.84 SGSRR32 pKa = 11.84 YY33 pKa = 8.12 RR34 pKa = 11.84 LVWRR38 pKa = 11.84 KK39 pKa = 9.57 SKK41 pKa = 10.91 NYY43 pKa = 10.71 NKK45 pKa = 10.17 FF46 pKa = 3.06
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.291
IPC2_protein 10.145
IPC_protein 11.096
Toseland 11.125
ProMoST 11.155
Dawson 11.184
Bjellqvist 10.994
Wikipedia 11.491
Rodwell 11.301
Grimsley 11.228
Solomon 11.447
Lehninger 11.389
Nozaki 11.096
DTASelect 10.994
Thurlkill 11.125
EMBOSS 11.564
Sillero 11.14
Patrickios 11.096
IPC_peptide 11.447
IPC2_peptide 10.16
IPC2.peptide.svr19 8.242
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
165
0
165
38393
46
1554
232.7
26.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.894 ± 0.258
0.31 ± 0.047
7.014 ± 0.176
8.934 ± 0.381
3.73 ± 0.151
6.413 ± 0.394
1.399 ± 0.091
6.806 ± 0.154
7.78 ± 0.283
8.291 ± 0.231
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.253 ± 0.111
6.699 ± 0.144
2.993 ± 0.157
3.188 ± 0.148
3.615 ± 0.146
6.657 ± 0.266
6.577 ± 0.256
6.272 ± 0.182
0.906 ± 0.069
5.269 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here