Neorhizobium sp. S3-V5DH

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Neorhizobium; unclassified Neorhizobium

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5607 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R3XFL1|A0A4R3XFL1_9RHIZ DeoR family transcriptional regulator OS=Neorhizobium sp. S3-V5DH OX=2485166 GN=EDE09_10649 PE=4 SV=1
MM1 pKa = 7.24TAMDD5 pKa = 5.66KK6 pKa = 9.45ITEE9 pKa = 4.53EE10 pKa = 3.76IAHH13 pKa = 6.7FIGLFHH19 pKa = 6.12TTVEE23 pKa = 4.29EE24 pKa = 3.81ARR26 pKa = 11.84QRR28 pKa = 11.84DD29 pKa = 3.76AYY31 pKa = 11.35LDD33 pKa = 3.56FSYY36 pKa = 9.8YY37 pKa = 6.59TQKK40 pKa = 11.25DD41 pKa = 3.62HH42 pKa = 7.03LHH44 pKa = 5.95TEE46 pKa = 4.25PFISSTFDD54 pKa = 2.9APYY57 pKa = 10.34EE58 pKa = 3.97FLGFDD63 pKa = 3.48PDD65 pKa = 3.67VFYY68 pKa = 10.98KK69 pKa = 10.8APAYY73 pKa = 9.65LRR75 pKa = 11.84PLEE78 pKa = 4.63HH79 pKa = 7.27PSFASWQHH87 pKa = 5.79HH88 pKa = 5.67PPILVVRR95 pKa = 11.84DD96 pKa = 3.9DD97 pKa = 4.03SPYY100 pKa = 10.01FPPEE104 pKa = 3.63QTEE107 pKa = 4.3EE108 pKa = 4.15ILPGARR114 pKa = 11.84IGQAFTVPPIEE125 pKa = 4.6PPGSVVNYY133 pKa = 9.66IIQAASLSDD142 pKa = 4.17DD143 pKa = 3.44DD144 pKa = 4.29TFNVGGSNLEE154 pKa = 3.95FHH156 pKa = 7.64PDD158 pKa = 3.64PVNDD162 pKa = 3.79AALLKK167 pKa = 10.75AADD170 pKa = 4.81DD171 pKa = 4.35VLSHH175 pKa = 6.72APIGHH180 pKa = 7.09PEE182 pKa = 4.06MPGSSAEE189 pKa = 4.38LIDD192 pKa = 4.59VINDD196 pKa = 2.89IAFQLKK202 pKa = 10.36ALVAAPGSPMEE213 pKa = 4.24TFIEE217 pKa = 4.23HH218 pKa = 6.08SPVIEE223 pKa = 3.81GVYY226 pKa = 10.77VNGEE230 pKa = 4.14LVEE233 pKa = 4.54DD234 pKa = 4.11APKK237 pKa = 10.99LEE239 pKa = 5.43DD240 pKa = 3.62YY241 pKa = 11.01FSFDD245 pKa = 3.52DD246 pKa = 4.41EE247 pKa = 4.63EE248 pKa = 4.27NAEE251 pKa = 4.23EE252 pKa = 4.56NDD254 pKa = 3.67AEE256 pKa = 4.52SPADD260 pKa = 3.77EE261 pKa = 5.81DD262 pKa = 4.98ADD264 pKa = 3.87NDD266 pKa = 4.2DD267 pKa = 5.21AVGADD272 pKa = 3.74ADD274 pKa = 4.58EE275 pKa = 5.86DD276 pKa = 4.33DD277 pKa = 4.95GPPPNVVISEE287 pKa = 4.56DD288 pKa = 3.53GSITIDD294 pKa = 3.37NSVEE298 pKa = 4.18LIAGDD303 pKa = 3.5NTVVNQAVVKK313 pKa = 10.02NLWTGATVTAVVGDD327 pKa = 3.91HH328 pKa = 6.3VEE330 pKa = 3.89INAIVQTNAIWDD342 pKa = 3.87TDD344 pKa = 4.53SITSAIDD351 pKa = 3.08GWNSEE356 pKa = 4.32DD357 pKa = 4.71SPNEE361 pKa = 3.74LFNIATFEE369 pKa = 4.06RR370 pKa = 11.84TDD372 pKa = 3.63PLEE375 pKa = 4.51EE376 pKa = 4.65GSGDD380 pKa = 3.28QAAAGGFPSAWAITEE395 pKa = 3.94IQGDD399 pKa = 3.98LLITNWLEE407 pKa = 3.83QYY409 pKa = 11.58VFMTDD414 pKa = 2.77NDD416 pKa = 3.75VGILSSSGVTTSVIAGDD433 pKa = 3.69NTSVNQTSIFEE444 pKa = 4.64LGFSFDD450 pKa = 4.69LIIVGGSLYY459 pKa = 10.48DD460 pKa = 3.59ANIIHH465 pKa = 5.38QTNVLFDD472 pKa = 4.03NDD474 pKa = 3.36VVGAVSGFEE483 pKa = 4.07TTGQGSIGSSGNLLWNQAHH502 pKa = 7.36IYY504 pKa = 9.7NVGGADD510 pKa = 3.49RR511 pKa = 11.84FGEE514 pKa = 4.3LPSAYY519 pKa = 10.17LDD521 pKa = 3.88AANDD525 pKa = 3.6MAAGGRR531 pKa = 11.84DD532 pKa = 3.44LSKK535 pKa = 11.25GVLTDD540 pKa = 3.46PAFAGLGGLRR550 pKa = 11.84VLYY553 pKa = 10.44ISGDD557 pKa = 3.64FLNIQYY563 pKa = 10.31ISQTNIVGDD572 pKa = 3.75SDD574 pKa = 4.42QIALAMNALDD584 pKa = 4.44PQTDD588 pKa = 3.82AAWTVSTGANALINNAAILDD608 pKa = 3.92FDD610 pKa = 4.67SFGHH614 pKa = 6.04TYY616 pKa = 10.76VGGQQYY622 pKa = 10.38SEE624 pKa = 4.02EE625 pKa = 4.23TLFQAEE631 pKa = 5.14LISHH635 pKa = 5.91QPDD638 pKa = 4.5LLTGDD643 pKa = 4.43PDD645 pKa = 4.03TLVNEE650 pKa = 4.34AVVFLDD656 pKa = 5.31DD657 pKa = 4.83SMLDD661 pKa = 3.68GEE663 pKa = 4.49FEE665 pKa = 4.42EE666 pKa = 4.95MGVTPAEE673 pKa = 4.07QDD675 pKa = 3.03NGHH678 pKa = 5.66YY679 pKa = 10.61QNDD682 pKa = 4.18GLQHH686 pKa = 5.34VLGG689 pKa = 4.52

Molecular weight: 74.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3XBB3|A0A4R3XBB3_9RHIZ 2-keto-4-pentenoate hydratase OS=Neorhizobium sp. S3-V5DH OX=2485166 GN=EDE09_11018 PE=4 SV=1
MM1 pKa = 7.32RR2 pKa = 11.84QLAKK6 pKa = 10.35FLSATGRR13 pKa = 11.84RR14 pKa = 11.84LRR16 pKa = 11.84TLGKK20 pKa = 9.81VVSHH24 pKa = 6.69FFRR27 pKa = 11.84KK28 pKa = 9.77GKK30 pKa = 10.34LGLNIAGGVVTT41 pKa = 5.52

Molecular weight: 4.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5607

0

5607

1719071

25

2831

306.6

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.603 ± 0.045

0.798 ± 0.008

5.561 ± 0.027

5.995 ± 0.032

3.982 ± 0.02

8.321 ± 0.028

2.022 ± 0.015

5.72 ± 0.027

3.774 ± 0.026

9.977 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.015

2.852 ± 0.018

4.972 ± 0.025

3.096 ± 0.019

6.741 ± 0.029

5.783 ± 0.023

5.23 ± 0.019

7.288 ± 0.026

1.313 ± 0.014

2.333 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski