Monascus purpureus (Red mold) (Monascus anka)
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8080 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A507QS44|A0A507QS44_MONPU Amidase (Fragment) OS=Monascus purpureus OX=5098 GN=MPDQ_000374 PE=3 SV=1
MM1 pKa = 7.75 PPLAHH6 pKa = 7.06 LLSAFLRR13 pKa = 11.84 IAEE16 pKa = 4.17 ISFAAIVAGIIGNFLYY32 pKa = 10.51 EE33 pKa = 4.27 FEE35 pKa = 5.24 LFSGDD40 pKa = 3.31 WIDD43 pKa = 4.43 ARR45 pKa = 11.84 WIYY48 pKa = 10.7 TEE50 pKa = 4.26 VIAGISIVLGLILVIPFATSFFAWPLDD77 pKa = 3.33 ILIALSWFAAFGILVDD93 pKa = 3.7 GAEE96 pKa = 5.01 RR97 pKa = 11.84 LDD99 pKa = 4.49 CDD101 pKa = 4.15 GNPYY105 pKa = 9.55 WVIFLGDD112 pKa = 3.95 SFCDD116 pKa = 3.0 EE117 pKa = 4.07 WKK119 pKa = 10.59 AAEE122 pKa = 4.26 AFCFLSGVAWIVSGFVVSSPLGQSITGLVVSDD154 pKa = 5.03 KK155 pKa = 10.99 II156 pKa = 4.05
Molecular weight: 17.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.948
IPC_protein 3.872
Toseland 3.668
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.795
Nozaki 3.986
DTASelect 4.19
Thurlkill 3.732
EMBOSS 3.808
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.889
Protein with the highest isoelectric point:
>tr|A0A507R2I8|A0A507R2I8_MONPU CHY-type domain-containing protein OS=Monascus purpureus OX=5098 GN=MPDQ_007514 PE=4 SV=1
MM1 pKa = 7.8 RR2 pKa = 11.84 GAIAPIPGDD11 pKa = 3.44 GKK13 pKa = 10.42 IDD15 pKa = 3.46 IRR17 pKa = 11.84 ILKK20 pKa = 9.56 PPLRR24 pKa = 11.84 AFAVGYY30 pKa = 10.16 LSSTAPRR37 pKa = 11.84 LVSYY41 pKa = 7.49 LTRR44 pKa = 11.84 SRR46 pKa = 11.84 KK47 pKa = 9.89 NKK49 pKa = 9.1 VGQKK53 pKa = 9.93 RR54 pKa = 11.84 RR55 pKa = 11.84 FEE57 pKa = 4.17 EE58 pKa = 4.08 LAEE61 pKa = 4.14 LLIKK65 pKa = 9.51 DD66 pKa = 3.95 TRR68 pKa = 11.84 FHH70 pKa = 7.01 AFPSFLALLVGGSTIIPVLAQRR92 pKa = 11.84 LAASIATRR100 pKa = 11.84 FRR102 pKa = 11.84 LRR104 pKa = 11.84 RR105 pKa = 11.84 DD106 pKa = 3.59 VITSSAFVRR115 pKa = 11.84 LTRR118 pKa = 11.84 FISALLSAWFCFRR131 pKa = 11.84 ILNRR135 pKa = 11.84 RR136 pKa = 11.84 HH137 pKa = 6.61 DD138 pKa = 3.98 RR139 pKa = 11.84 LQRR142 pKa = 11.84 HH143 pKa = 5.69 YY144 pKa = 10.99 GGQIEE149 pKa = 4.6 DD150 pKa = 3.57 ASEE153 pKa = 4.05 KK154 pKa = 10.58 QSASDD159 pKa = 3.47 PTDD162 pKa = 3.62 SIEE165 pKa = 3.77 QRR167 pKa = 11.84 QRR169 pKa = 11.84 RR170 pKa = 11.84 SPEE173 pKa = 3.83 FAGSTLDD180 pKa = 3.55 LTVLVATRR188 pKa = 11.84 AADD191 pKa = 3.53 AVACIGWARR200 pKa = 11.84 WRR202 pKa = 11.84 AWRR205 pKa = 11.84 MTRR208 pKa = 11.84 NRR210 pKa = 11.84 WTLAEE215 pKa = 4.14 SVAPKK220 pKa = 10.5 VADD223 pKa = 3.48 AGIFVLSSAVIMWAWFYY240 pKa = 11.26 LPEE243 pKa = 4.67 RR244 pKa = 11.84 LPRR247 pKa = 11.84 SYY249 pKa = 11.26 GRR251 pKa = 11.84 WIDD254 pKa = 3.38 EE255 pKa = 4.07 AAQVDD260 pKa = 3.58 ARR262 pKa = 11.84 LIEE265 pKa = 4.05 VLRR268 pKa = 11.84 RR269 pKa = 11.84 ARR271 pKa = 11.84 RR272 pKa = 11.84 GEE274 pKa = 3.82 FVYY277 pKa = 10.39 GKK279 pKa = 8.74 DD280 pKa = 2.95 TGQAPFLQTMCKK292 pKa = 10.18 DD293 pKa = 3.88 YY294 pKa = 10.58 GWPMVWGDD302 pKa = 3.54 PAKK305 pKa = 9.98 TIPLPCEE312 pKa = 4.11 VVHH315 pKa = 6.36 MGYY318 pKa = 10.76 GPNCEE323 pKa = 3.63 IYY325 pKa = 10.24 AARR328 pKa = 11.84 RR329 pKa = 11.84 FLKK332 pKa = 9.69 TFKK335 pKa = 10.64 FGFWTYY341 pKa = 10.68 FPLQAALRR349 pKa = 11.84 ARR351 pKa = 11.84 GLRR354 pKa = 11.84 SMRR357 pKa = 11.84 TLVRR361 pKa = 11.84 IAFDD365 pKa = 3.62 ASRR368 pKa = 11.84 SSAFLALFVTLFYY381 pKa = 11.35 YY382 pKa = 8.94 SVCLARR388 pKa = 11.84 TRR390 pKa = 11.84 LGPKK394 pKa = 9.87 VFDD397 pKa = 4.27 KK398 pKa = 11.57 NKK400 pKa = 7.1 VTPMMWDD407 pKa = 2.87 SGLCVGAGCLMCGWSVLVEE426 pKa = 3.86 KK427 pKa = 10.69 ASRR430 pKa = 11.84 RR431 pKa = 11.84 RR432 pKa = 11.84 EE433 pKa = 3.7 LSLFVAPRR441 pKa = 11.84 AAATLLPRR449 pKa = 11.84 VYY451 pKa = 10.58 DD452 pKa = 3.03 KK453 pKa = 11.04 QYY455 pKa = 9.13 QHH457 pKa = 7.02 RR458 pKa = 11.84 EE459 pKa = 3.72 RR460 pKa = 11.84 IAFAVCAAILSTCLQEE476 pKa = 4.21 RR477 pKa = 11.84 PEE479 pKa = 4.0 MVRR482 pKa = 11.84 GFFRR486 pKa = 11.84 KK487 pKa = 9.73 IGARR491 pKa = 11.84 LIAA494 pKa = 4.7
Molecular weight: 56.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.677
IPC_protein 10.613
Toseland 10.657
ProMoST 10.438
Dawson 10.76
Bjellqvist 10.526
Wikipedia 11.008
Rodwell 10.877
Grimsley 10.818
Solomon 10.891
Lehninger 10.862
Nozaki 10.657
DTASelect 10.511
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.701
Patrickios 10.54
IPC_peptide 10.906
IPC2_peptide 9.75
IPC2.peptide.svr19 8.547
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8080
0
8080
4068950
51
8221
503.6
55.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.212 ± 0.024
1.155 ± 0.01
5.727 ± 0.017
6.347 ± 0.025
3.728 ± 0.016
6.686 ± 0.023
2.393 ± 0.01
4.982 ± 0.018
4.812 ± 0.023
9.035 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.039 ± 0.009
3.711 ± 0.013
6.09 ± 0.03
3.964 ± 0.017
6.416 ± 0.024
8.631 ± 0.032
5.751 ± 0.018
6.175 ± 0.02
1.372 ± 0.009
2.773 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here