Capybara microvirus Cap3_SP_394
Average proteome isoelectric point is 5.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5B0|A0A4P8W5B0_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_394 OX=2585444 PE=4 SV=1
MM1 pKa = 7.64 VDD3 pKa = 3.65 DD4 pKa = 5.44 FLVEE8 pKa = 4.28 SLPQEE13 pKa = 4.47 DD14 pKa = 4.03 GSYY17 pKa = 10.56 ISVSVRR23 pKa = 11.84 NQQKK27 pKa = 10.99 DD28 pKa = 3.29 YY29 pKa = 11.53 GDD31 pKa = 3.83 YY32 pKa = 11.43 DD33 pKa = 3.94 NYY35 pKa = 11.1 TPDD38 pKa = 3.38 ALSRR42 pKa = 11.84 TGQTLPTMMMPDD54 pKa = 3.44 GSSRR58 pKa = 11.84 LEE60 pKa = 4.0 ASSSLSSLLAQGEE73 pKa = 4.56 QILSQPDD80 pKa = 3.71 STPVTTDD87 pKa = 2.79 NNGG90 pKa = 2.86
Molecular weight: 9.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.075
IPC2_protein 3.668
IPC_protein 3.617
Toseland 3.414
ProMoST 3.834
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.617
Rodwell 3.452
Grimsley 3.325
Solomon 3.605
Lehninger 3.567
Nozaki 3.783
DTASelect 4.024
Thurlkill 3.49
EMBOSS 3.63
Sillero 3.757
Patrickios 3.058
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.728
Protein with the highest isoelectric point:
>tr|A0A4P8W4Q6|A0A4P8W4Q6_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_394 OX=2585444 PE=3 SV=1
MM1 pKa = 7.47 YY2 pKa = 9.46 STIHH6 pKa = 6.23 LKK8 pKa = 10.95 SNAKK12 pKa = 8.78 SWRR15 pKa = 11.84 PLADD19 pKa = 2.93 AAYY22 pKa = 9.71 YY23 pKa = 9.87 DD24 pKa = 4.39 LPRR27 pKa = 11.84 PTTFASKK34 pKa = 8.28 TAARR38 pKa = 11.84 YY39 pKa = 9.79 GYY41 pKa = 9.22 ICRR44 pKa = 11.84 IKK46 pKa = 10.85 AQLQDD51 pKa = 2.99 TWRR54 pKa = 11.84 SGGKK58 pKa = 10.07 AFFYY62 pKa = 10.59 TLTHH66 pKa = 6.26 AQHH69 pKa = 6.52 SLPHH73 pKa = 6.3 FYY75 pKa = 10.79 GIPVHH80 pKa = 7.08 DD81 pKa = 3.55 YY82 pKa = 10.49 RR83 pKa = 11.84 VINEE87 pKa = 4.3 FFNCSGFSKK96 pKa = 10.84 VLMRR100 pKa = 11.84 RR101 pKa = 11.84 HH102 pKa = 5.46 NCRR105 pKa = 11.84 IKK107 pKa = 10.35 YY108 pKa = 9.67 FLVSEE113 pKa = 4.71 LGNGGEE119 pKa = 4.07 SHH121 pKa = 6.08 QYY123 pKa = 9.85 KK124 pKa = 10.23 GVRR127 pKa = 11.84 GLLNNPHH134 pKa = 5.6 YY135 pKa = 10.68 HH136 pKa = 6.26 VIFFLTSLDD145 pKa = 3.56 DD146 pKa = 4.07 HH147 pKa = 7.05 LSVDD151 pKa = 3.33 ITADD155 pKa = 3.26 QLKK158 pKa = 10.36 YY159 pKa = 10.4 CVQKK163 pKa = 10.57 YY164 pKa = 6.97 WSGLPDD170 pKa = 3.51 YY171 pKa = 11.11 GVDD174 pKa = 3.46 PKK176 pKa = 11.1 VDD178 pKa = 4.39 RR179 pKa = 11.84 IRR181 pKa = 11.84 DD182 pKa = 3.51 NKK184 pKa = 10.11 AALGAWIKK192 pKa = 10.85 QYY194 pKa = 11.14 GKK196 pKa = 10.94 YY197 pKa = 10.02 GLAWPGSLHH206 pKa = 6.34 NGEE209 pKa = 5.27 ILNQPDD215 pKa = 3.21 SDD217 pKa = 3.89 FANKK221 pKa = 9.27 YY222 pKa = 6.82 VCKK225 pKa = 10.53 YY226 pKa = 8.6 VVKK229 pKa = 10.71 NHH231 pKa = 5.47 ATYY234 pKa = 8.82 TQAIRR239 pKa = 11.84 LRR241 pKa = 11.84 SEE243 pKa = 4.12 LNRR246 pKa = 11.84 LVYY249 pKa = 10.35 GRR251 pKa = 11.84 YY252 pKa = 9.7 DD253 pKa = 3.37 KK254 pKa = 11.41 EE255 pKa = 3.95 GVYY258 pKa = 9.95 IDD260 pKa = 4.04 GLLEE264 pKa = 4.55 KK265 pKa = 10.1 MMHH268 pKa = 6.18 NPWQAYY274 pKa = 10.1 AFMHH278 pKa = 6.74 RR279 pKa = 11.84 PKK281 pKa = 10.38 KK282 pKa = 8.07 WQCDD286 pKa = 3.65 VYY288 pKa = 11.09 RR289 pKa = 11.84 DD290 pKa = 3.97 YY291 pKa = 12.03 NDD293 pKa = 3.22 GSEE296 pKa = 4.12 VDD298 pKa = 3.12 KK299 pKa = 11.43 SGYY302 pKa = 8.3 TDD304 pKa = 4.4 QEE306 pKa = 4.48 ACLSWCLYY314 pKa = 9.54 RR315 pKa = 11.84 LKK317 pKa = 10.86 SEE319 pKa = 4.13 WQRR322 pKa = 11.84 HH323 pKa = 4.24 CTEE326 pKa = 4.49 HH327 pKa = 6.43 YY328 pKa = 9.43 EE329 pKa = 4.15 KK330 pKa = 10.87 FIVRR334 pKa = 11.84 QLALFRR340 pKa = 11.84 SRR342 pKa = 11.84 FAPHH346 pKa = 5.83 IRR348 pKa = 11.84 ASHH351 pKa = 5.6 GVGVSALLEE360 pKa = 4.23 VDD362 pKa = 5.63 DD363 pKa = 6.53 DD364 pKa = 4.91 DD365 pKa = 4.18 LTIDD369 pKa = 3.37 VRR371 pKa = 11.84 EE372 pKa = 4.09 NGVVTKK378 pKa = 10.6 HH379 pKa = 6.55 RR380 pKa = 11.84 ITGYY384 pKa = 9.79 LARR387 pKa = 11.84 KK388 pKa = 8.96 KK389 pKa = 10.76 LYY391 pKa = 10.46 DD392 pKa = 3.61 VVEE395 pKa = 4.41 APGNQTRR402 pKa = 11.84 YY403 pKa = 9.3 VRR405 pKa = 11.84 NSWYY409 pKa = 10.12 KK410 pKa = 9.97 QWLKK414 pKa = 11.31 DD415 pKa = 3.53 HH416 pKa = 6.6 LAEE419 pKa = 6.11 KK420 pKa = 9.9 IDD422 pKa = 3.37 KK423 pKa = 10.5 CEE425 pKa = 3.84 RR426 pKa = 11.84 RR427 pKa = 11.84 LRR429 pKa = 11.84 DD430 pKa = 3.03 LWYY433 pKa = 8.75 NTDD436 pKa = 3.41 INLLSKK442 pKa = 9.69 ICEE445 pKa = 4.09 YY446 pKa = 11.33 NEE448 pKa = 3.49 VDD450 pKa = 3.73 VIPRR454 pKa = 11.84 VVSDD458 pKa = 3.91 FRR460 pKa = 11.84 DD461 pKa = 3.36 LAIYY465 pKa = 10.04 ACVYY469 pKa = 9.98 RR470 pKa = 11.84 YY471 pKa = 9.82 RR472 pKa = 11.84 HH473 pKa = 5.63 FHH475 pKa = 6.89 RR476 pKa = 11.84 DD477 pKa = 3.41 DD478 pKa = 3.95 PYY480 pKa = 11.61 EE481 pKa = 3.98 LYY483 pKa = 10.54 NYY485 pKa = 9.63 KK486 pKa = 10.12 KK487 pKa = 10.73 YY488 pKa = 11.05 YY489 pKa = 7.57 EE490 pKa = 4.09 QCIDD494 pKa = 4.64 APLEE498 pKa = 4.22 DD499 pKa = 4.34 TGCSYY504 pKa = 11.5 AVYY507 pKa = 10.6 QRR509 pKa = 11.84 MGSFGQEE516 pKa = 3.62 GTDD519 pKa = 3.48 YY520 pKa = 11.13 IPTNFVWPHH529 pKa = 5.59 MEE531 pKa = 4.18 ADD533 pKa = 3.78 MEE535 pKa = 4.35 VLDD538 pKa = 5.19 MISDD542 pKa = 3.8 YY543 pKa = 11.44 LFAQIDD549 pKa = 3.64 INRR552 pKa = 11.84 DD553 pKa = 3.46 KK554 pKa = 10.85 EE555 pKa = 4.31 WEE557 pKa = 4.24 LKK559 pKa = 9.08 SHH561 pKa = 6.09 QMYY564 pKa = 10.23 NQQQLAYY571 pKa = 9.92 DD572 pKa = 3.87
Molecular weight: 67.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.985
IPC2_protein 7.19
IPC_protein 7.088
Toseland 6.942
ProMoST 7.571
Dawson 7.732
Bjellqvist 8.039
Wikipedia 7.658
Rodwell 7.732
Grimsley 7.059
Solomon 7.761
Lehninger 7.775
Nozaki 8.156
DTASelect 7.907
Thurlkill 7.936
EMBOSS 7.966
Sillero 8.2
Patrickios 3.579
IPC_peptide 7.761
IPC2_peptide 7.337
IPC2.peptide.svr19 7.233
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1600
90
575
400.0
45.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.375 ± 0.967
1.188 ± 0.576
7.313 ± 0.686
3.938 ± 0.781
3.25 ± 0.855
6.563 ± 0.573
2.75 ± 0.724
5.625 ± 0.657
4.5 ± 0.933
8.438 ± 0.377
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.375 ± 0.42
5.063 ± 0.33
3.375 ± 0.456
6.188 ± 0.922
5.25 ± 0.739
8.0 ± 1.593
5.75 ± 0.954
5.438 ± 0.322
1.75 ± 0.378
5.875 ± 1.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here