Amino acid dipepetide frequency for Streptomyces sp. DSM 40868

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.561AlaAla: 21.561 ± 0.163	1.105AlaCys: 1.105 ± 0.021	8.443AlaAsp: 8.443 ± 0.067	8.913AlaGlu: 8.913 ± 0.09	3.57AlaPhe: 3.57 ± 0.04	13.912AlaGly: 13.912 ± 0.092	3.113AlaHis: 3.113 ± 0.038	3.141AlaIle: 3.141 ± 0.042	2.652AlaLys: 2.652 ± 0.048	15.013AlaLeu: 15.013 ± 0.105	2.307AlaMet: 2.307 ± 0.034	1.768AlaAsn: 1.768 ± 0.026	7.503AlaPro: 7.503 ± 0.081	3.566AlaGln: 3.566 ± 0.046	11.311AlaArg: 11.311 ± 0.097	5.514AlaSer: 5.514 ± 0.048	6.994AlaThr: 6.994 ± 0.053	12.436AlaVal: 12.436 ± 0.091	1.883AlaTrp: 1.883 ± 0.027	2.889AlaTyr: 2.889 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	1.198CysAla: 1.198 ± 0.023	0.095CysCys: 0.095 ± 0.007	0.463CysAsp: 0.463 ± 0.014	0.428CysGlu: 0.428 ± 0.014	0.225CysPhe: 0.225 ± 0.01	0.957CysGly: 0.957 ± 0.02	0.204CysHis: 0.204 ± 0.01	0.142CysIle: 0.142 ± 0.007	0.095CysLys: 0.095 ± 0.006	0.798CysLeu: 0.798 ± 0.019	0.123CysMet: 0.123 ± 0.006	0.127CysAsn: 0.127 ± 0.008	0.505CysPro: 0.505 ± 0.015	0.162CysGln: 0.162 ± 0.007	0.639CysArg: 0.639 ± 0.015	0.422CysSer: 0.422 ± 0.014	0.5CysThr: 0.5 ± 0.015	0.681CysVal: 0.681 ± 0.019	0.128CysTrp: 0.128 ± 0.006	0.157CysTyr: 0.157 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.574AspAla: 7.574 ± 0.07	0.418AspCys: 0.418 ± 0.013	3.49AspAsp: 3.49 ± 0.043	3.592AspGlu: 3.592 ± 0.041	1.657AspPhe: 1.657 ± 0.027	6.466AspGly: 6.466 ± 0.067	1.522AspHis: 1.522 ± 0.024	1.874AspIle: 1.874 ± 0.031	1.173AspLys: 1.173 ± 0.026	6.19AspLeu: 6.19 ± 0.051	0.807AspMet: 0.807 ± 0.017	0.929AspAsn: 0.929 ± 0.02	4.688AspPro: 4.688 ± 0.045	1.491AspGln: 1.491 ± 0.027	5.181AspArg: 5.181 ± 0.055	2.448AspSer: 2.448 ± 0.034	3.409AspThr: 3.409 ± 0.039	4.51AspVal: 4.51 ± 0.045	1.077AspTrp: 1.077 ± 0.021	1.135AspTyr: 1.135 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	7.237GluAla: 7.237 ± 0.079	0.364GluCys: 0.364 ± 0.013	2.863GluAsp: 2.863 ± 0.034	3.72GluGlu: 3.72 ± 0.052	1.447GluPhe: 1.447 ± 0.024	4.158GluGly: 4.158 ± 0.043	1.558GluHis: 1.558 ± 0.028	2.157GluIle: 2.157 ± 0.032	1.357GluLys: 1.357 ± 0.031	6.651GluLeu: 6.651 ± 0.065	0.822GluMet: 0.822 ± 0.019	0.993GluAsn: 0.993 ± 0.024	3.423GluPro: 3.423 ± 0.043	2.158GluGln: 2.158 ± 0.03	5.784GluArg: 5.784 ± 0.059	2.324GluSer: 2.324 ± 0.029	2.928GluThr: 2.928 ± 0.034	4.375GluVal: 4.375 ± 0.042	0.763GluTrp: 0.763 ± 0.016	1.115GluTyr: 1.115 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	3.657PheAla: 3.657 ± 0.048	0.265PheCys: 0.265 ± 0.011	1.945PheAsp: 1.945 ± 0.033	1.406PheGlu: 1.406 ± 0.03	0.888PhePhe: 0.888 ± 0.021	2.935PheGly: 2.935 ± 0.037	0.647PheHis: 0.647 ± 0.015	0.637PheIle: 0.637 ± 0.017	0.495PheLys: 0.495 ± 0.015	2.664PheLeu: 2.664 ± 0.036	0.372PheMet: 0.372 ± 0.012	0.514PheAsn: 0.514 ± 0.016	1.415PhePro: 1.415 ± 0.025	0.686PheGln: 0.686 ± 0.015	1.913PheArg: 1.913 ± 0.03	1.389PheSer: 1.389 ± 0.023	2.111PheThr: 2.111 ± 0.031	2.178PheVal: 2.178 ± 0.031	0.412PheTrp: 0.412 ± 0.012	0.575PheTyr: 0.575 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	11.222GlyAla: 11.222 ± 0.085	0.872GlyCys: 0.872 ± 0.021	5.206GlyAsp: 5.206 ± 0.05	5.076GlyGlu: 5.076 ± 0.046	2.829GlyPhe: 2.829 ± 0.037	8.795GlyGly: 8.795 ± 0.078	2.547GlyHis: 2.547 ± 0.035	3.34GlyIle: 3.34 ± 0.038	2.302GlyLys: 2.302 ± 0.038	9.538GlyLeu: 9.538 ± 0.075	1.904GlyMet: 1.904 ± 0.029	1.74GlyAsn: 1.74 ± 0.035	5.501GlyPro: 5.501 ± 0.054	2.603GlyGln: 2.603 ± 0.035	8.278GlyArg: 8.278 ± 0.068	5.002GlySer: 5.002 ± 0.058	6.812GlyThr: 6.812 ± 0.064	7.472GlyVal: 7.472 ± 0.063	1.754GlyTrp: 1.754 ± 0.026	2.372GlyTyr: 2.372 ± 0.035	0.0GlyXaa: 0.0 ± 0.0
His	2.87HisAla: 2.87 ± 0.037	0.208HisCys: 0.208 ± 0.008	1.387HisAsp: 1.387 ± 0.023	1.201HisGlu: 1.201 ± 0.025	0.656HisPhe: 0.656 ± 0.015	2.53HisGly: 2.53 ± 0.029	0.728HisHis: 0.728 ± 0.021	0.69HisIle: 0.69 ± 0.018	0.357HisLys: 0.357 ± 0.013	2.568HisLeu: 2.568 ± 0.036	0.346HisMet: 0.346 ± 0.012	0.366HisAsn: 0.366 ± 0.013	1.966HisPro: 1.966 ± 0.027	0.677HisGln: 0.677 ± 0.018	2.275HisArg: 2.275 ± 0.031	1.034HisSer: 1.034 ± 0.018	1.499HisThr: 1.499 ± 0.024	1.68HisVal: 1.68 ± 0.028	0.426HisTrp: 0.426 ± 0.012	0.515HisTyr: 0.515 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.527IleAla: 4.527 ± 0.05	0.271IleCys: 0.271 ± 0.01	2.073IleAsp: 2.073 ± 0.03	1.846IleGlu: 1.846 ± 0.027	0.574IlePhe: 0.574 ± 0.015	3.337IleGly: 3.337 ± 0.041	0.588IleHis: 0.588 ± 0.015	0.774IleIle: 0.774 ± 0.02	0.686IleLys: 0.686 ± 0.015	2.294IleLeu: 2.294 ± 0.034	0.398IleMet: 0.398 ± 0.014	0.61IleAsn: 0.61 ± 0.018	1.692IlePro: 1.692 ± 0.026	0.681IleGln: 0.681 ± 0.018	2.286IleArg: 2.286 ± 0.029	1.47IleSer: 1.47 ± 0.026	2.179IleThr: 2.179 ± 0.031	2.543IleVal: 2.543 ± 0.041	0.343IleTrp: 0.343 ± 0.011	0.5IleTyr: 0.5 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.816LysAla: 2.816 ± 0.048	0.117LysCys: 0.117 ± 0.008	1.318LysAsp: 1.318 ± 0.03	1.157LysGlu: 1.157 ± 0.026	0.432LysPhe: 0.432 ± 0.014	1.755LysGly: 1.755 ± 0.031	0.396LysHis: 0.396 ± 0.012	0.788LysIle: 0.788 ± 0.019	0.815LysLys: 0.815 ± 0.025	1.843LysLeu: 1.843 ± 0.031	0.347LysMet: 0.347 ± 0.013	0.492LysAsn: 0.492 ± 0.018	1.262LysPro: 1.262 ± 0.029	0.657LysGln: 0.657 ± 0.02	1.381LysArg: 1.381 ± 0.024	1.07LysSer: 1.07 ± 0.027	1.234LysThr: 1.234 ± 0.027	1.799LysVal: 1.799 ± 0.033	0.275LysTrp: 0.275 ± 0.012	0.44LysTyr: 0.44 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	15.696LeuAla: 15.696 ± 0.114	0.907LeuCys: 0.907 ± 0.019	6.709LeuAsp: 6.709 ± 0.053	4.734LeuGlu: 4.734 ± 0.054	2.654LeuPhe: 2.654 ± 0.039	9.338LeuGly: 9.338 ± 0.074	2.327LeuHis: 2.327 ± 0.037	3.092LeuIle: 3.092 ± 0.04	1.958LeuLys: 1.958 ± 0.032	11.66LeuLeu: 11.66 ± 0.105	1.56LeuMet: 1.56 ± 0.027	1.603LeuAsn: 1.603 ± 0.028	6.81LeuPro: 6.81 ± 0.061	2.067LeuGln: 2.067 ± 0.033	9.167LeuArg: 9.167 ± 0.075	5.156LeuSer: 5.156 ± 0.047	7.187LeuThr: 7.187 ± 0.059	8.675LeuVal: 8.675 ± 0.072	1.384LeuTrp: 1.384 ± 0.025	1.93LeuTyr: 1.93 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.176MetAla: 2.176 ± 0.038	0.131MetCys: 0.131 ± 0.008	0.822MetAsp: 0.822 ± 0.018	0.704MetGlu: 0.704 ± 0.016	0.444MetPhe: 0.444 ± 0.015	1.176MetGly: 1.176 ± 0.026	0.331MetHis: 0.331 ± 0.011	0.595MetIle: 0.595 ± 0.016	0.39MetLys: 0.39 ± 0.012	1.548MetLeu: 1.548 ± 0.025	0.261MetMet: 0.261 ± 0.01	0.397MetAsn: 0.397 ± 0.013	1.081MetPro: 1.081 ± 0.021	0.381MetGln: 0.381 ± 0.013	1.364MetArg: 1.364 ± 0.023	1.223MetSer: 1.223 ± 0.021	1.539MetThr: 1.539 ± 0.02	1.202MetVal: 1.202 ± 0.023	0.227MetTrp: 0.227 ± 0.011	0.317MetTyr: 0.317 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.059AsnAla: 2.059 ± 0.035	0.16AsnCys: 0.16 ± 0.009	0.863AsnAsp: 0.863 ± 0.018	0.746AsnGlu: 0.746 ± 0.018	0.458AsnPhe: 0.458 ± 0.014	1.821AsnGly: 1.821 ± 0.039	0.391AsnHis: 0.391 ± 0.013	0.571AsnIle: 0.571 ± 0.016	0.385AsnLys: 0.385 ± 0.014	1.585AsnLeu: 1.585 ± 0.026	0.291AsnMet: 0.291 ± 0.009	0.386AsnAsn: 0.386 ± 0.013	1.293AsnPro: 1.293 ± 0.025	0.478AsnGln: 0.478 ± 0.015	1.217AsnArg: 1.217 ± 0.024	0.871AsnSer: 0.871 ± 0.021	1.099AsnThr: 1.099 ± 0.025	1.342AsnVal: 1.342 ± 0.024	0.284AsnTrp: 0.284 ± 0.01	0.402AsnTyr: 0.402 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	9.737ProAla: 9.737 ± 0.098	0.37ProCys: 0.37 ± 0.012	4.75ProAsp: 4.75 ± 0.038	4.444ProGlu: 4.444 ± 0.051	1.52ProPhe: 1.52 ± 0.026	7.31ProGly: 7.31 ± 0.069	1.514ProHis: 1.514 ± 0.026	1.106ProIle: 1.106 ± 0.021	1.091ProLys: 1.091 ± 0.024	5.591ProLeu: 5.591 ± 0.053	0.94ProMet: 0.94 ± 0.019	0.874ProAsn: 0.874 ± 0.018	3.682ProPro: 3.682 ± 0.053	1.626ProGln: 1.626 ± 0.029	4.346ProArg: 4.346 ± 0.043	2.978ProSer: 2.978 ± 0.038	3.037ProThr: 3.037 ± 0.041	5.733ProVal: 5.733 ± 0.056	0.906ProTrp: 0.906 ± 0.017	1.516ProTyr: 1.516 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.695GlnAla: 3.695 ± 0.04	0.168GlnCys: 0.168 ± 0.009	1.365GlnAsp: 1.365 ± 0.026	1.384GlnGlu: 1.384 ± 0.027	0.644GlnPhe: 0.644 ± 0.019	2.255GlnGly: 2.255 ± 0.031	0.659GlnHis: 0.659 ± 0.017	0.961GlnIle: 0.961 ± 0.022	0.589GlnLys: 0.589 ± 0.016	2.826GlnLeu: 2.826 ± 0.037	0.422GlnMet: 0.422 ± 0.013	0.469GlnAsn: 0.469 ± 0.015	1.655GlnPro: 1.655 ± 0.035	1.111GlnGln: 1.111 ± 0.032	2.33GlnArg: 2.33 ± 0.033	1.169GlnSer: 1.169 ± 0.022	1.318GlnThr: 1.318 ± 0.023	2.265GlnVal: 2.265 ± 0.036	0.465GlnTrp: 0.465 ± 0.014	0.587GlnTyr: 0.587 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	10.667ArgAla: 10.667 ± 0.08	0.625ArgCys: 0.625 ± 0.015	4.44ArgAsp: 4.44 ± 0.051	4.936ArgGlu: 4.936 ± 0.056	2.408ArgPhe: 2.408 ± 0.028	5.924ArgGly: 5.924 ± 0.052	2.378ArgHis: 2.378 ± 0.037	3.229ArgIle: 3.229 ± 0.036	1.625ArgLys: 1.625 ± 0.03	9.76ArgLeu: 9.76 ± 0.085	1.752ArgMet: 1.752 ± 0.025	1.314ArgAsn: 1.314 ± 0.025	5.636ArgPro: 5.636 ± 0.059	2.424ArgGln: 2.424 ± 0.032	8.308ArgArg: 8.308 ± 0.081	3.848ArgSer: 3.848 ± 0.041	5.678ArgThr: 5.678 ± 0.055	6.195ArgVal: 6.195 ± 0.058	1.428ArgTrp: 1.428 ± 0.021	1.949ArgTyr: 1.949 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	6.486SerAla: 6.486 ± 0.058	0.408SerCys: 0.408 ± 0.014	2.468SerAsp: 2.468 ± 0.03	2.1SerGlu: 2.1 ± 0.033	1.462SerPhe: 1.462 ± 0.03	5.697SerGly: 5.697 ± 0.058	0.977SerHis: 0.977 ± 0.021	1.218SerIle: 1.218 ± 0.025	0.915SerLys: 0.915 ± 0.022	4.713SerLeu: 4.713 ± 0.044	0.988SerMet: 0.988 ± 0.022	0.746SerAsn: 0.746 ± 0.02	3.076SerPro: 3.076 ± 0.036	1.072SerGln: 1.072 ± 0.025	3.583SerArg: 3.583 ± 0.038	2.478SerSer: 2.478 ± 0.04	2.813SerThr: 2.813 ± 0.034	4.151SerVal: 4.151 ± 0.043	0.875SerTrp: 0.875 ± 0.019	1.207SerTyr: 1.207 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	9.392ThrAla: 9.392 ± 0.077	0.435ThrCys: 0.435 ± 0.015	3.74ThrAsp: 3.74 ± 0.042	3.384ThrGlu: 3.384 ± 0.04	1.602ThrPhe: 1.602 ± 0.026	7.219ThrGly: 7.219 ± 0.063	1.285ThrHis: 1.285 ± 0.024	1.588ThrIle: 1.588 ± 0.026	1.085ThrLys: 1.085 ± 0.028	5.806ThrLeu: 5.806 ± 0.05	0.856ThrMet: 0.856 ± 0.019	0.938ThrAsn: 0.938 ± 0.02	4.331ThrPro: 4.331 ± 0.043	1.266ThrGln: 1.266 ± 0.023	4.176ThrArg: 4.176 ± 0.043	2.984ThrSer: 2.984 ± 0.035	3.877ThrThr: 3.877 ± 0.057	6.169ThrVal: 6.169 ± 0.055	0.926ThrTrp: 0.926 ± 0.021	1.421ThrTyr: 1.421 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	10.424ValAla: 10.424 ± 0.084	0.793ValCys: 0.793 ± 0.019	4.762ValAsp: 4.762 ± 0.049	4.439ValGlu: 4.439 ± 0.046	2.467ValPhe: 2.467 ± 0.04	6.119ValGly: 6.119 ± 0.059	1.979ValHis: 1.979 ± 0.027	2.751ValIle: 2.751 ± 0.038	1.67ValLys: 1.67 ± 0.032	9.584ValLeu: 9.584 ± 0.073	1.3ValMet: 1.3 ± 0.023	1.621ValAsn: 1.621 ± 0.027	5.606ValPro: 5.606 ± 0.062	1.966ValGln: 1.966 ± 0.03	7.637ValArg: 7.637 ± 0.058	4.184ValSer: 4.184 ± 0.043	5.913ValThr: 5.913 ± 0.051	7.711ValVal: 7.711 ± 0.068	1.188ValTrp: 1.188 ± 0.023	1.656ValTyr: 1.656 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.742TrpAla: 1.742 ± 0.029	0.161TrpCys: 0.161 ± 0.007	0.871TrpAsp: 0.871 ± 0.02	0.75TrpGlu: 0.75 ± 0.016	0.517TrpPhe: 0.517 ± 0.013	1.08TrpGly: 1.08 ± 0.02	0.425TrpHis: 0.425 ± 0.013	0.526TrpIle: 0.526 ± 0.015	0.353TrpLys: 0.353 ± 0.014	1.799TrpLeu: 1.799 ± 0.032	0.264TrpMet: 0.264 ± 0.01	0.406TrpAsn: 0.406 ± 0.014	0.828TrpPro: 0.828 ± 0.018	0.639TrpGln: 0.639 ± 0.016	1.402TrpArg: 1.402 ± 0.025	0.941TrpSer: 0.941 ± 0.022	1.057TrpThr: 1.057 ± 0.019	0.982TrpVal: 0.982 ± 0.022	0.349TrpTrp: 0.349 ± 0.012	0.367TrpTyr: 0.367 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.858TyrAla: 2.858 ± 0.036	0.177TyrCys: 0.177 ± 0.009	1.649TyrAsp: 1.649 ± 0.032	1.241TyrGlu: 1.241 ± 0.023	0.65TyrPhe: 0.65 ± 0.013	2.381TyrGly: 2.381 ± 0.033	0.41TyrHis: 0.41 ± 0.013	0.488TyrIle: 0.488 ± 0.015	0.401TyrLys: 0.401 ± 0.013	2.18TyrLeu: 2.18 ± 0.032	0.248TyrMet: 0.248 ± 0.009	0.41TyrAsn: 0.41 ± 0.013	1.116TyrPro: 1.116 ± 0.024	0.626TyrGln: 0.626 ± 0.017	1.947TyrArg: 1.947 ± 0.035	0.931TyrSer: 0.931 ± 0.02	1.299TyrThr: 1.299 ± 0.028	1.671TyrVal: 1.671 ± 0.025	0.366TyrTrp: 0.366 ± 0.012	0.477TyrTyr: 0.477 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7715 proteins (2524438 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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