Cyanophage PP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Wumptrevirus; Phormidium virus PP

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5PRB0|U5PRB0_9CAUD Uncharacterized protein OS=Cyanophage PP OX=434346 GN=PP_08 PE=4 SV=1
MM1 pKa = 7.24NCIALTLSVLLCRR14 pKa = 11.84QVSPVGVLTATIEE27 pKa = 4.22LYY29 pKa = 10.71EE30 pKa = 4.57PLPQTTLTLDD40 pKa = 3.49EE41 pKa = 4.72CSSRR45 pKa = 11.84SEE47 pKa = 4.27GFTLRR52 pKa = 11.84PDD54 pKa = 3.83SEE56 pKa = 4.36CQEE59 pKa = 4.48LLTTLDD65 pKa = 3.43NTSEE69 pKa = 4.11VAYY72 pKa = 10.52GNTKK76 pKa = 7.89QQQ78 pKa = 2.97

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5PRM8|U5PRM8_9CAUD Terminase OS=Cyanophage PP OX=434346 GN=PP_37 PE=4 SV=1
MM1 pKa = 7.52TLAQLQVTIMDD12 pKa = 4.67DD13 pKa = 3.35KK14 pKa = 11.55QGIAWLISKK23 pKa = 8.54VINGIKK29 pKa = 10.34DD30 pKa = 3.51LLAKK34 pKa = 10.23RR35 pKa = 11.84KK36 pKa = 9.71RR37 pKa = 11.84KK38 pKa = 9.5

Molecular weight: 4.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

13156

38

1712

320.9

35.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.609 ± 0.366

0.699 ± 0.143

6.666 ± 0.212

5.921 ± 0.426

3.39 ± 0.271

6.469 ± 0.292

1.619 ± 0.247

3.443 ± 0.159

5.496 ± 0.388

9.007 ± 0.193

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.824 ± 0.175

4.5 ± 0.21

4.401 ± 0.525

5.382 ± 0.558

5.777 ± 0.319

6.697 ± 0.33

7.768 ± 0.51

7.806 ± 0.264

1.11 ± 0.124

4.416 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski