Bacillus phage Bobb

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Agatevirus; Bacillus virus Bobb

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 243 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076G7R5|A0A076G7R5_9CAUD Uncharacterized protein OS=Bacillus phage Bobb OX=1527469 PE=4 SV=1
MM1 pKa = 7.43SNSVLQKK8 pKa = 10.14MFIDD12 pKa = 4.23RR13 pKa = 11.84YY14 pKa = 9.34DD15 pKa = 3.64YY16 pKa = 11.31QMHH19 pKa = 5.21VQCDD23 pKa = 3.6IIDD26 pKa = 4.48GYY28 pKa = 11.1DD29 pKa = 3.48VYY31 pKa = 11.09CTDD34 pKa = 3.1TRR36 pKa = 11.84QAEE39 pKa = 4.13DD40 pKa = 3.56DD41 pKa = 3.84VLIVLRR47 pKa = 11.84ADD49 pKa = 3.68DD50 pKa = 5.62LIFSSLEE57 pKa = 4.03GEE59 pKa = 4.66TIWYY63 pKa = 6.64TIKK66 pKa = 11.03DD67 pKa = 3.76GAEE70 pKa = 3.72VIAINEE76 pKa = 4.21PEE78 pKa = 4.21DD79 pKa = 4.08EE80 pKa = 4.16EE81 pKa = 6.41DD82 pKa = 3.54EE83 pKa = 4.37

Molecular weight: 9.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076G7B5|A0A076G7B5_9CAUD Uncharacterized protein OS=Bacillus phage Bobb OX=1527469 PE=4 SV=1
MM1 pKa = 7.47ARR3 pKa = 11.84RR4 pKa = 11.84SAFALSEE11 pKa = 4.37LKK13 pKa = 10.56CHH15 pKa = 5.35QCGNITQIQRR25 pKa = 11.84RR26 pKa = 11.84TAVLKK31 pKa = 10.62KK32 pKa = 10.19GGHH35 pKa = 5.99IKK37 pKa = 10.31HH38 pKa = 6.57LYY40 pKa = 8.72CWRR43 pKa = 11.84CKK45 pKa = 10.06RR46 pKa = 11.84DD47 pKa = 3.26TRR49 pKa = 11.84QVEE52 pKa = 3.82LSRR55 pKa = 11.84IEE57 pKa = 4.24KK58 pKa = 10.06YY59 pKa = 10.73

Molecular weight: 7.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

243

0

243

47260

14

1251

194.5

22.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.49 ± 0.194

0.753 ± 0.059

6.384 ± 0.143

8.366 ± 0.298

3.999 ± 0.103

5.95 ± 0.207

2.052 ± 0.099

6.151 ± 0.14

7.493 ± 0.242

8.314 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.554 ± 0.091

5.205 ± 0.129

3.091 ± 0.144

3.72 ± 0.13

4.725 ± 0.129

6.473 ± 0.186

6.014 ± 0.21

6.915 ± 0.151

1.024 ± 0.051

4.327 ± 0.105
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski