Pseudoalteromonas ruthenica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3470 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F4Q0M3|A0A0F4Q0M3_9GAMM Bifunctional protein FolD OS=Pseudoalteromonas ruthenica OX=151081 GN=folD PE=3 SV=1
MM1 pKa = 7.65KK2 pKa = 9.01KK3 pKa = 8.22TLLGLSIAAALVSLPSFAEE22 pKa = 3.79VRR24 pKa = 11.84INGFASIVGGQTLDD38 pKa = 5.16DD39 pKa = 5.62DD40 pKa = 4.28DD41 pKa = 4.21TLYY44 pKa = 11.16GYY46 pKa = 11.07DD47 pKa = 3.59NDD49 pKa = 4.64FNMEE53 pKa = 4.01QEE55 pKa = 4.61SKK57 pKa = 10.78FALQVSADD65 pKa = 3.63LQEE68 pKa = 4.61RR69 pKa = 11.84LSATAQVIARR79 pKa = 11.84GEE81 pKa = 4.0NDD83 pKa = 2.83FDD85 pKa = 6.18AEE87 pKa = 4.27FEE89 pKa = 4.36WAYY92 pKa = 9.39LTYY95 pKa = 10.23EE96 pKa = 4.07ISDD99 pKa = 3.69NSQLSAGKK107 pKa = 8.75MRR109 pKa = 11.84IPFYY113 pKa = 10.42RR114 pKa = 11.84YY115 pKa = 9.92SDD117 pKa = 3.85FLDD120 pKa = 2.91VGYY123 pKa = 10.55AYY125 pKa = 10.49RR126 pKa = 11.84WVRR129 pKa = 11.84PPKK132 pKa = 10.23SVYY135 pKa = 9.93NLNFSTYY142 pKa = 9.73EE143 pKa = 3.96GLSYY147 pKa = 10.64LYY149 pKa = 10.44NSNIGEE155 pKa = 4.24WDD157 pKa = 3.34SSIQLMYY164 pKa = 11.05GSVNEE169 pKa = 5.11DD170 pKa = 2.59IAAVTEE176 pKa = 4.13SDD178 pKa = 3.27NVEE181 pKa = 3.81INDD184 pKa = 3.47AFGFNWTLSKK194 pKa = 11.11DD195 pKa = 3.19WFTARR200 pKa = 11.84AAYY203 pKa = 9.35ILSEE207 pKa = 4.02VSIDD211 pKa = 3.69VSNSAQLSALFSGLSGYY228 pKa = 10.02GLNEE232 pKa = 3.58QRR234 pKa = 11.84QDD236 pKa = 3.53LAVEE240 pKa = 4.15EE241 pKa = 4.7DD242 pKa = 3.42DD243 pKa = 5.78SYY245 pKa = 11.75FAGIGFGIDD254 pKa = 3.29YY255 pKa = 11.2NNFLFDD261 pKa = 4.47AEE263 pKa = 4.32YY264 pKa = 8.75TQYY267 pKa = 10.88EE268 pKa = 4.27IEE270 pKa = 4.65DD271 pKa = 3.84SFLAKK276 pKa = 10.05QSQYY280 pKa = 9.69YY281 pKa = 10.44ASVGYY286 pKa = 10.28RR287 pKa = 11.84IDD289 pKa = 3.29DD290 pKa = 3.65WTVHH294 pKa = 5.32FTYY297 pKa = 10.65EE298 pKa = 4.53NNDD301 pKa = 3.78DD302 pKa = 4.37EE303 pKa = 5.42NDD305 pKa = 3.63SSEE308 pKa = 4.93LNTIPAQIQTPNGPINVSTNPSDD331 pKa = 3.64PNAPLLRR338 pKa = 11.84DD339 pKa = 3.52VTNFALLSQRR349 pKa = 11.84AEE351 pKa = 4.24SNTYY355 pKa = 9.74TIGARR360 pKa = 11.84YY361 pKa = 9.43NFHH364 pKa = 7.22PSAAFKK370 pKa = 10.44IDD372 pKa = 3.22VSRR375 pKa = 11.84FEE377 pKa = 5.66DD378 pKa = 5.11DD379 pKa = 3.03ITNTEE384 pKa = 3.61VDD386 pKa = 4.41LVSMGVDD393 pKa = 3.41LVFF396 pKa = 5.28

Molecular weight: 44.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F4PYH9|A0A0F4PYH9_9GAMM Uncharacterized protein OS=Pseudoalteromonas ruthenica OX=151081 GN=TW72_08440 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3470

0

3470

1147547

34

2488

330.7

36.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.57 ± 0.044

1.013 ± 0.016

5.661 ± 0.035

6.034 ± 0.045

4.008 ± 0.028

6.689 ± 0.039

2.525 ± 0.028

5.682 ± 0.029

4.783 ± 0.042

10.505 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.022

4.018 ± 0.027

3.838 ± 0.026

5.589 ± 0.049

4.764 ± 0.028

6.441 ± 0.035

5.095 ± 0.028

6.925 ± 0.037

1.213 ± 0.016

3.24 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski