Anthracocystis flocculosa PF-1

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Anthracocystis; Anthracocystis flocculosa

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4425 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A061H899|A0A061H899_9BASI G-patch domain-containing protein OS=Anthracocystis flocculosa PF-1 OX=1277687 GN=PFL1_06900 PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 10.22FSAATALALATVASASPLLKK22 pKa = 10.35RR23 pKa = 11.84QQDD26 pKa = 3.66PNATGGAAGAGGDD39 pKa = 3.79NSTSSSGGSEE49 pKa = 3.96NGTSTNDD56 pKa = 3.33PTPIPLNLNDD66 pKa = 4.08TQILQYY72 pKa = 11.58ALTLEE77 pKa = 4.22HH78 pKa = 7.13LEE80 pKa = 3.9AAFYY84 pKa = 10.36NQSLQQLGEE93 pKa = 4.23DD94 pKa = 3.99AFSQAGFNASTRR106 pKa = 11.84NQLYY110 pKa = 10.71SIGRR114 pKa = 11.84DD115 pKa = 3.17EE116 pKa = 4.39AAHH119 pKa = 6.71VDD121 pKa = 4.52FLTQALGPDD130 pKa = 3.29AVGACTYY137 pKa = 10.62NFSAVQDD144 pKa = 3.96VPSFLATAQVLEE156 pKa = 4.39GVGVSAYY163 pKa = 9.7IGAARR168 pKa = 11.84NITSKK173 pKa = 11.07DD174 pKa = 3.68YY175 pKa = 10.94LVSAASILTVEE186 pKa = 4.63SRR188 pKa = 11.84HH189 pKa = 5.6ASYY192 pKa = 11.05INSQVSADD200 pKa = 3.81GNVVPSPYY208 pKa = 9.41DD209 pKa = 3.27TPLDD213 pKa = 3.85FNQVYY218 pKa = 10.61SLAAPFITEE227 pKa = 3.97CPRR230 pKa = 11.84NQSLPFSPFPAASLSPAGGADD251 pKa = 2.99ASSNNGFFTPGSQVTVSYY269 pKa = 11.27DD270 pKa = 3.13SGNSTNGGSGAMGNNSSSGMDD291 pKa = 3.24GQTYY295 pKa = 9.94LAIIQGPTPPVFLPFTQDD313 pKa = 3.1QPVTLPSNLTGGQIYY328 pKa = 10.61AIVTSSNSSVTDD340 pKa = 3.86DD341 pKa = 3.51NTIAGPAVAYY351 pKa = 10.0INVPLPEE358 pKa = 5.01APSASASASSSAGSTGAAGAPGASEE383 pKa = 4.17TGSMSMMPTASSAAGSSTQTNGGAAGAGPMATSSGSSGMMPTDD426 pKa = 3.41AAGSSSAAPSATASGISSEE445 pKa = 4.47LSAAQASASAFTGSVGSASAAPAPTGSAA473 pKa = 3.31

Molecular weight: 46.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A061HBC6|A0A061HBC6_9BASI Stc1 domain-containing protein OS=Anthracocystis flocculosa PF-1 OX=1277687 GN=PFL1_03090 PE=4 SV=1
MM1 pKa = 7.72PRR3 pKa = 11.84TSLLAGLAASSRR15 pKa = 11.84SRR17 pKa = 11.84MLRR20 pKa = 11.84PSTPRR25 pKa = 11.84IFSAPSATAPTAPTTPTPAVSAHH48 pKa = 6.3PRR50 pKa = 11.84TPGASTSLLRR60 pKa = 11.84SFNSLSLSSGVARR73 pKa = 11.84SAASPLSLLSALSRR87 pKa = 11.84PTLSSSSSTSPLATATVASARR108 pKa = 11.84SVTYY112 pKa = 10.1GSEE115 pKa = 4.1YY116 pKa = 10.47QPSQRR121 pKa = 11.84IRR123 pKa = 11.84KK124 pKa = 8.61RR125 pKa = 11.84RR126 pKa = 11.84HH127 pKa = 4.7GFLARR132 pKa = 11.84IKK134 pKa = 9.15TKK136 pKa = 10.66NGRR139 pKa = 11.84KK140 pKa = 7.47TIARR144 pKa = 11.84RR145 pKa = 11.84RR146 pKa = 11.84AKK148 pKa = 10.49GKK150 pKa = 10.61AKK152 pKa = 10.39LSHH155 pKa = 6.44

Molecular weight: 16.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4425

0

4425

2781444

54

5730

628.6

67.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.86 ± 0.039

0.94 ± 0.014

6.222 ± 0.029

5.563 ± 0.032

2.96 ± 0.018

8.028 ± 0.039

2.407 ± 0.016

3.487 ± 0.022

3.968 ± 0.031

8.485 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.799 ± 0.01

2.645 ± 0.018

6.549 ± 0.034

4.187 ± 0.03

7.017 ± 0.032

9.498 ± 0.049

5.536 ± 0.021

5.644 ± 0.025

1.133 ± 0.012

2.063 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski