Marivivens niveibacter
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2990 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A251WZ41|A0A251WZ41_9RHOB 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase OS=Marivivens niveibacter OX=1930667 GN=hisA PE=3 SV=1
MM1 pKa = 7.17 YY2 pKa = 7.79 RR3 pKa = 11.84 TLLATSAVLAISATASVAADD23 pKa = 3.36 NAAVLDD29 pKa = 4.33 TYY31 pKa = 11.95 ANIAEE36 pKa = 4.46 AGYY39 pKa = 11.13 ADD41 pKa = 4.01 SLATAKK47 pKa = 10.41 VLQAAVNEE55 pKa = 4.41 LVANPSEE62 pKa = 4.32 DD63 pKa = 3.15 TLAAARR69 pKa = 11.84 SAWLDD74 pKa = 3.05 ARR76 pKa = 11.84 VPYY79 pKa = 10.14 QQTEE83 pKa = 3.98 VFRR86 pKa = 11.84 FGNAIVDD93 pKa = 3.67 DD94 pKa = 4.12 WEE96 pKa = 4.58 GLVNAWPLDD105 pKa = 3.65 EE106 pKa = 5.54 GLIDD110 pKa = 4.71 YY111 pKa = 11.04 VDD113 pKa = 3.49 ASYY116 pKa = 11.35 GGPTDD121 pKa = 3.64 EE122 pKa = 6.08 NEE124 pKa = 3.77 FAALNVIANPTFTVAGVDD142 pKa = 3.07 IDD144 pKa = 3.87 ATNITPEE151 pKa = 5.03 LIADD155 pKa = 4.21 SLNEE159 pKa = 3.81 ADD161 pKa = 5.08 GIEE164 pKa = 4.37 TNVARR169 pKa = 11.84 GYY171 pKa = 10.46 HH172 pKa = 6.42 AIEE175 pKa = 4.05 FLLWGQDD182 pKa = 4.15 LNGTDD187 pKa = 3.88 AGAGDD192 pKa = 4.54 RR193 pKa = 11.84 PYY195 pKa = 11.13 TDD197 pKa = 3.85 YY198 pKa = 11.96 ADD200 pKa = 5.85 GDD202 pKa = 3.98 DD203 pKa = 4.35 CTNGNCDD210 pKa = 3.02 RR211 pKa = 11.84 RR212 pKa = 11.84 GDD214 pKa = 3.92 YY215 pKa = 11.03 LIAATDD221 pKa = 4.02 LLVSDD226 pKa = 5.35 LEE228 pKa = 4.75 FITAAWAAEE237 pKa = 4.17 GEE239 pKa = 4.25 ARR241 pKa = 11.84 AEE243 pKa = 3.92 LTADD247 pKa = 3.44 EE248 pKa = 4.4 NAGIIAILTGMGSLSYY264 pKa = 11.07 GEE266 pKa = 4.04 QAGEE270 pKa = 3.95 RR271 pKa = 11.84 MRR273 pKa = 11.84 LGLMLNDD280 pKa = 4.01 PEE282 pKa = 4.81 EE283 pKa = 4.14 EE284 pKa = 4.02 HH285 pKa = 7.9 DD286 pKa = 3.95 CFSDD290 pKa = 3.4 NTHH293 pKa = 6.28 NSHH296 pKa = 6.64 YY297 pKa = 10.44 YY298 pKa = 10.56 DD299 pKa = 3.53 GLGVQNVYY307 pKa = 10.27 LGSYY311 pKa = 8.36 TRR313 pKa = 11.84 VDD315 pKa = 3.52 GTVVSGPSLSEE326 pKa = 3.64 LVAAANPEE334 pKa = 3.9 LDD336 pKa = 3.98 AEE338 pKa = 4.48 LTAKK342 pKa = 10.85 LNTTVAALGDD352 pKa = 3.52 IVEE355 pKa = 4.3 AAEE358 pKa = 4.21 NGFSYY363 pKa = 11.68 DD364 pKa = 3.26 MMLEE368 pKa = 4.02 RR369 pKa = 11.84 GNAEE373 pKa = 3.65 GEE375 pKa = 4.24 ALVMGAVNGLIDD387 pKa = 3.51 QTRR390 pKa = 11.84 SIEE393 pKa = 4.23 KK394 pKa = 10.02 VVAALGLDD402 pKa = 4.24 AIAFEE407 pKa = 5.46 GSDD410 pKa = 4.15 SLDD413 pKa = 3.48 NPDD416 pKa = 5.36 AVFQQ420 pKa = 4.04
Molecular weight: 44.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.668
IPC_protein 3.694
Toseland 3.478
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.605
Rodwell 3.516
Grimsley 3.376
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.012
Thurlkill 3.516
EMBOSS 3.617
Sillero 3.808
Patrickios 1.303
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.739
Protein with the highest isoelectric point:
>tr|A0A251WZ97|A0A251WZ97_9RHOB Uncharacterized protein OS=Marivivens niveibacter OX=1930667 GN=BVC71_11240 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.55 ILNSRR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.31 KK41 pKa = 10.65 LSAA44 pKa = 3.91
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2990
0
2990
935407
32
3956
312.8
34.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.373 ± 0.058
0.891 ± 0.014
6.605 ± 0.044
5.797 ± 0.042
3.841 ± 0.029
8.22 ± 0.045
1.951 ± 0.023
5.993 ± 0.032
3.613 ± 0.04
9.288 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.966 ± 0.025
3.22 ± 0.025
4.645 ± 0.029
3.349 ± 0.026
5.867 ± 0.046
5.432 ± 0.034
5.859 ± 0.051
7.368 ± 0.041
1.323 ± 0.017
2.4 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here