Sulfolobus monocaudavirus SMV4
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N9P771|A0A0N9P771_9VIRU Uncharacterized protein OS=Sulfolobus monocaudavirus SMV4 OX=1732178 PE=4 SV=1
MM1 pKa = 7.81 GILEE5 pKa = 4.49 DD6 pKa = 3.79 VQNALAYY13 pKa = 7.85 YY14 pKa = 10.09 QKK16 pKa = 9.71 TAVDD20 pKa = 4.12 EE21 pKa = 4.51 KK22 pKa = 11.11 DD23 pKa = 3.11 TLYY26 pKa = 11.29 LNNIYY31 pKa = 10.4 SQLYY35 pKa = 9.24 YY36 pKa = 10.13 ILSVEE41 pKa = 4.02 NRR43 pKa = 11.84 PLNDD47 pKa = 3.45 TEE49 pKa = 4.82 FDD51 pKa = 3.88 SLMKK55 pKa = 10.8 ALGINDD61 pKa = 3.64 DD62 pKa = 3.95 TQLVAEE68 pKa = 4.56 YY69 pKa = 11.09 QNMTDD74 pKa = 3.76 DD75 pKa = 3.76 QKK77 pKa = 11.63 KK78 pKa = 9.09 QLLMQILQMIDD89 pKa = 3.05 NVMKK93 pKa = 10.78 NIHH96 pKa = 5.53 TMVNYY101 pKa = 10.42
Molecular weight: 11.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.895
IPC2_protein 4.19
IPC_protein 4.113
Toseland 3.91
ProMoST 4.279
Dawson 4.101
Bjellqvist 4.253
Wikipedia 4.05
Rodwell 3.948
Grimsley 3.821
Solomon 4.088
Lehninger 4.05
Nozaki 4.228
DTASelect 4.457
Thurlkill 3.961
EMBOSS 4.05
Sillero 4.24
Patrickios 1.977
IPC_peptide 4.088
IPC2_peptide 4.215
IPC2.peptide.svr19 4.127
Protein with the highest isoelectric point:
>tr|A0A0N9P9T6|A0A0N9P9T6_9VIRU Uncharacterized protein OS=Sulfolobus monocaudavirus SMV4 OX=1732178 PE=4 SV=1
MM1 pKa = 7.71 GIYY4 pKa = 9.25 XKK6 pKa = 9.77 IDD8 pKa = 4.34 KK9 pKa = 9.96 ILKK12 pKa = 9.68 NSTIPGIIKK21 pKa = 9.97 IAVILLLLRR30 pKa = 11.84 DD31 pKa = 3.62 KK32 pKa = 10.92 RR33 pKa = 11.84 RR34 pKa = 11.84 NEE36 pKa = 4.21 AIQLYY41 pKa = 10.26 NSYY44 pKa = 10.63 PEE46 pKa = 4.24 YY47 pKa = 9.86 NTSFNHH53 pKa = 7.01 FIVEE57 pKa = 4.14 YY58 pKa = 9.71 EE59 pKa = 4.05 VGKK62 pKa = 10.17 NRR64 pKa = 11.84 YY65 pKa = 7.43 VRR67 pKa = 11.84 RR68 pKa = 11.84 LTHH71 pKa = 6.39 KK72 pKa = 10.19 YY73 pKa = 10.43 LRR75 pKa = 11.84 TLLFLRR81 pKa = 11.84 SARR84 pKa = 11.84 LYY86 pKa = 9.97 NFKK89 pKa = 10.58 IILL92 pKa = 3.91
Molecular weight: 10.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.262
IPC2_protein 9.94
IPC_protein 10.394
Toseland 10.292
ProMoST 10.043
Dawson 10.496
Bjellqvist 10.204
Wikipedia 10.701
Rodwell 10.847
Grimsley 10.584
Solomon 10.54
Lehninger 10.496
Nozaki 10.248
DTASelect 10.204
Thurlkill 10.335
EMBOSS 10.687
Sillero 10.409
Patrickios 10.496
IPC_peptide 10.526
IPC2_peptide 8.96
IPC2.peptide.svr19 8.629
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
0
68
15051
45
1180
221.3
24.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.481 ± 0.295
0.551 ± 0.091
4.638 ± 0.44
6.817 ± 0.875
4.438 ± 0.294
5.063 ± 0.423
0.89 ± 0.095
8.225 ± 0.359
6.618 ± 0.643
10.451 ± 0.438
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.973 ± 0.181
6.398 ± 0.342
3.967 ± 0.331
4.764 ± 0.587
2.824 ± 0.282
7.734 ± 0.569
6.352 ± 0.526
6.823 ± 0.435
0.664 ± 0.111
4.976 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here