Seal anellovirus 4
Average proteome isoelectric point is 9.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7TXL6|A0A0A7TXL6_9VIRU Capsid protein OS=Seal anellovirus 4 OX=1566011 PE=3 SV=1
MM1 pKa = 7.0 AWYY4 pKa = 8.39 WRR6 pKa = 11.84 WRR8 pKa = 11.84 TRR10 pKa = 11.84 RR11 pKa = 11.84 WRR13 pKa = 11.84 WRR15 pKa = 11.84 PRR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 FVRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 FYY28 pKa = 9.93 PRR30 pKa = 11.84 RR31 pKa = 11.84 ANRR34 pKa = 11.84 LRR36 pKa = 11.84 YY37 pKa = 8.12 NRR39 pKa = 11.84 RR40 pKa = 11.84 WVRR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 FRR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 HH54 pKa = 5.63 APFTSKK60 pKa = 10.81 VLLWNPSRR68 pKa = 11.84 IVRR71 pKa = 11.84 CKK73 pKa = 10.27 IIGWHH78 pKa = 6.79 PGLFCNQFNTIDD90 pKa = 3.48 TCYY93 pKa = 11.14 VNAIEE98 pKa = 5.85 DD99 pKa = 3.76 KK100 pKa = 10.08 QTVQGFRR107 pKa = 11.84 GGGVAFLALTLNMLYY122 pKa = 10.07 RR123 pKa = 11.84 EE124 pKa = 4.79 HH125 pKa = 8.03 IFGRR129 pKa = 11.84 NFWTRR134 pKa = 11.84 SNEE137 pKa = 3.99 GFDD140 pKa = 3.34 LAQYY144 pKa = 10.36 RR145 pKa = 11.84 GTRR148 pKa = 11.84 VTFFPHH154 pKa = 5.27 YY155 pKa = 8.25 QLPYY159 pKa = 9.1 IVTWLRR165 pKa = 11.84 DD166 pKa = 3.13 FATPVDD172 pKa = 3.55 KK173 pKa = 10.69 HH174 pKa = 6.85 IQDD177 pKa = 3.58 MHH179 pKa = 5.84 PQRR182 pKa = 11.84 ALFGPNHH189 pKa = 6.32 KK190 pKa = 10.58 VILPRR195 pKa = 11.84 VFGRR199 pKa = 11.84 KK200 pKa = 9.09 RR201 pKa = 11.84 KK202 pKa = 7.18 WSLYY206 pKa = 9.51 IRR208 pKa = 11.84 PPPLNEE214 pKa = 3.9 RR215 pKa = 11.84 KK216 pKa = 8.87 WYY218 pKa = 11.37 SMVSWCNVVIAKK230 pKa = 10.21 IGISFINLTSPLLHH244 pKa = 6.32 QEE246 pKa = 4.34 SPFPAVFCGYY256 pKa = 8.63 TMQSGSFGEE265 pKa = 4.61 PPRR268 pKa = 11.84 CMLDD272 pKa = 3.3 PEE274 pKa = 4.67 GLPKK278 pKa = 10.25 VRR280 pKa = 11.84 EE281 pKa = 3.92 NPTWHH286 pKa = 6.09 VGNYY290 pKa = 9.08 NLPCCYY296 pKa = 10.01 RR297 pKa = 11.84 PLWDD301 pKa = 5.79 DD302 pKa = 3.35 GTDD305 pKa = 3.21 NYY307 pKa = 11.05 VLMNYY312 pKa = 7.17 TNKK315 pKa = 9.85 PPSVQGTYY323 pKa = 10.32 RR324 pKa = 11.84 PTGTGQEE331 pKa = 4.05 VLIWQAKK338 pKa = 7.79 NRR340 pKa = 11.84 HH341 pKa = 5.79 ALLAMVLRR349 pKa = 11.84 FNRR352 pKa = 11.84 IKK354 pKa = 10.64 RR355 pKa = 11.84 NYY357 pKa = 8.46 LCKK360 pKa = 10.12 PLATWQIFWPSVMGMYY376 pKa = 9.74 WYY378 pKa = 10.7 VDD380 pKa = 3.31 QARR383 pKa = 11.84 VLEE386 pKa = 4.4 GTVDD390 pKa = 3.95 TIVPDD395 pKa = 3.27 MSITVPEE402 pKa = 4.12 EE403 pKa = 3.96 LPTKK407 pKa = 8.63 TNRR410 pKa = 11.84 SWIIFTPPRR419 pKa = 11.84 GEE421 pKa = 3.76 NMAKK425 pKa = 9.56 IRR427 pKa = 11.84 EE428 pKa = 4.51 HH429 pKa = 6.01 IHH431 pKa = 6.16 GNNIMTWPTLPQVKK445 pKa = 8.73 TICSRR450 pKa = 11.84 IAGSAGFTIGYY461 pKa = 10.16 GEE463 pKa = 4.34 MPIKK467 pKa = 9.08 TANVPFFYY475 pKa = 10.29 KK476 pKa = 10.48 SYY478 pKa = 8.77 WRR480 pKa = 11.84 WGGSFADD487 pKa = 3.36 ADD489 pKa = 3.81 TRR491 pKa = 11.84 VRR493 pKa = 11.84 NPCVEE498 pKa = 4.25 LPMATGTDD506 pKa = 3.09 RR507 pKa = 11.84 LGVQIRR513 pKa = 11.84 DD514 pKa = 3.49 PATVSLANIHH524 pKa = 6.48 PWDD527 pKa = 4.53 LEE529 pKa = 4.13 NGSITKK535 pKa = 9.54 PAMLRR540 pKa = 11.84 ILSSILGPGSHH551 pKa = 7.04 LPGAPTADD559 pKa = 3.56 PQLPQAKK566 pKa = 9.32 EE567 pKa = 4.12 GEE569 pKa = 4.3 EE570 pKa = 4.13 LPEE573 pKa = 4.07 QSSSSGDD580 pKa = 3.06 SDD582 pKa = 3.78 TPTEE586 pKa = 4.21 EE587 pKa = 4.16 EE588 pKa = 4.42 PPTSEE593 pKa = 4.43 EE594 pKa = 3.99 EE595 pKa = 4.28 QEE597 pKa = 4.35 TSQVWRR603 pKa = 11.84 RR604 pKa = 11.84 KK605 pKa = 6.85 LQRR608 pKa = 11.84 MGKK611 pKa = 8.88 RR612 pKa = 11.84 LRR614 pKa = 11.84 RR615 pKa = 11.84 EE616 pKa = 3.47 QQYY619 pKa = 10.18 RR620 pKa = 11.84 RR621 pKa = 11.84 RR622 pKa = 11.84 VKK624 pKa = 10.32 QRR626 pKa = 11.84 LLEE629 pKa = 4.03 LSGYY633 pKa = 7.15 QHH635 pKa = 7.68 PSTQFMEE642 pKa = 4.41
Molecular weight: 75.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.677
IPC_protein 10.511
Toseland 10.409
ProMoST 10.189
Dawson 10.584
Bjellqvist 10.335
Wikipedia 10.818
Rodwell 10.73
Grimsley 10.672
Solomon 10.657
Lehninger 10.613
Nozaki 10.423
DTASelect 10.321
Thurlkill 10.452
EMBOSS 10.818
Sillero 10.511
Patrickios 10.292
IPC_peptide 10.657
IPC2_peptide 9.458
IPC2.peptide.svr19 8.518
Protein with the highest isoelectric point:
>tr|A0A0A7TXL6|A0A0A7TXL6_9VIRU Capsid protein OS=Seal anellovirus 4 OX=1566011 PE=3 SV=1
MM1 pKa = 7.0 AWYY4 pKa = 8.39 WRR6 pKa = 11.84 WRR8 pKa = 11.84 TRR10 pKa = 11.84 RR11 pKa = 11.84 WRR13 pKa = 11.84 WRR15 pKa = 11.84 PRR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 FVRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 FYY28 pKa = 9.93 PRR30 pKa = 11.84 RR31 pKa = 11.84 ANRR34 pKa = 11.84 LRR36 pKa = 11.84 YY37 pKa = 8.12 NRR39 pKa = 11.84 RR40 pKa = 11.84 WVRR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 FRR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 HH54 pKa = 5.63 APFTSKK60 pKa = 10.81 VLLWNPSRR68 pKa = 11.84 IVRR71 pKa = 11.84 CKK73 pKa = 10.27 IIGWHH78 pKa = 6.79 PGLFCNQFNTIDD90 pKa = 3.48 TCYY93 pKa = 11.14 VNAIEE98 pKa = 5.85 DD99 pKa = 3.76 KK100 pKa = 10.08 QTVQGFRR107 pKa = 11.84 GGGVAFLALTLNMLYY122 pKa = 10.07 RR123 pKa = 11.84 EE124 pKa = 4.79 HH125 pKa = 8.03 IFGRR129 pKa = 11.84 NFWTRR134 pKa = 11.84 SNEE137 pKa = 3.99 GFDD140 pKa = 3.34 LAQYY144 pKa = 10.36 RR145 pKa = 11.84 GTRR148 pKa = 11.84 VTFFPHH154 pKa = 5.27 YY155 pKa = 8.25 QLPYY159 pKa = 9.1 IVTWLRR165 pKa = 11.84 DD166 pKa = 3.13 FATPVDD172 pKa = 3.55 KK173 pKa = 10.69 HH174 pKa = 6.85 IQDD177 pKa = 3.58 MHH179 pKa = 5.84 PQRR182 pKa = 11.84 ALFGPNHH189 pKa = 6.32 KK190 pKa = 10.58 VILPRR195 pKa = 11.84 VFGRR199 pKa = 11.84 KK200 pKa = 9.09 RR201 pKa = 11.84 KK202 pKa = 7.18 WSLYY206 pKa = 9.51 IRR208 pKa = 11.84 PPPLNEE214 pKa = 3.9 RR215 pKa = 11.84 KK216 pKa = 8.87 WYY218 pKa = 11.37 SMVSWCNVVIAKK230 pKa = 10.21 IGISFINLTSPLLHH244 pKa = 6.32 QEE246 pKa = 4.34 SPFPAVFCGYY256 pKa = 8.63 TMQSGSFGEE265 pKa = 4.61 PPRR268 pKa = 11.84 CMLDD272 pKa = 3.3 PEE274 pKa = 4.67 GLPKK278 pKa = 10.25 VRR280 pKa = 11.84 EE281 pKa = 3.92 NPTWHH286 pKa = 6.09 VGNYY290 pKa = 9.08 NLPCCYY296 pKa = 10.01 RR297 pKa = 11.84 PLWDD301 pKa = 5.79 DD302 pKa = 3.35 GTDD305 pKa = 3.21 NYY307 pKa = 11.05 VLMNYY312 pKa = 7.17 TNKK315 pKa = 9.85 PPSVQGTYY323 pKa = 10.32 RR324 pKa = 11.84 PTGTGQEE331 pKa = 4.05 VLIWQAKK338 pKa = 7.79 NRR340 pKa = 11.84 HH341 pKa = 5.79 ALLAMVLRR349 pKa = 11.84 FNRR352 pKa = 11.84 IKK354 pKa = 10.64 RR355 pKa = 11.84 NYY357 pKa = 8.46 LCKK360 pKa = 10.12 PLATWQIFWPSVMGMYY376 pKa = 9.74 WYY378 pKa = 10.7 VDD380 pKa = 3.31 QARR383 pKa = 11.84 VLEE386 pKa = 4.4 GTVDD390 pKa = 3.95 TIVPDD395 pKa = 3.27 MSITVPEE402 pKa = 4.12 EE403 pKa = 3.96 LPTKK407 pKa = 8.63 TNRR410 pKa = 11.84 SWIIFTPPRR419 pKa = 11.84 GEE421 pKa = 3.76 NMAKK425 pKa = 9.56 IRR427 pKa = 11.84 EE428 pKa = 4.51 HH429 pKa = 6.01 IHH431 pKa = 6.16 GNNIMTWPTLPQVKK445 pKa = 8.73 TICSRR450 pKa = 11.84 IAGSAGFTIGYY461 pKa = 10.16 GEE463 pKa = 4.34 MPIKK467 pKa = 9.08 TANVPFFYY475 pKa = 10.29 KK476 pKa = 10.48 SYY478 pKa = 8.77 WRR480 pKa = 11.84 WGGSFADD487 pKa = 3.36 ADD489 pKa = 3.81 TRR491 pKa = 11.84 VRR493 pKa = 11.84 NPCVEE498 pKa = 4.25 LPMATGTDD506 pKa = 3.09 RR507 pKa = 11.84 LGVQIRR513 pKa = 11.84 DD514 pKa = 3.49 PATVSLANIHH524 pKa = 6.48 PWDD527 pKa = 4.53 LEE529 pKa = 4.13 NGSITKK535 pKa = 9.54 PAMLRR540 pKa = 11.84 ILSSILGPGSHH551 pKa = 7.04 LPGAPTADD559 pKa = 3.56 PQLPQAKK566 pKa = 9.32 EE567 pKa = 4.12 GEE569 pKa = 4.3 EE570 pKa = 4.13 LPEE573 pKa = 4.07 QSSSSGDD580 pKa = 3.06 SDD582 pKa = 3.78 TPTEE586 pKa = 4.21 EE587 pKa = 4.16 EE588 pKa = 4.42 PPTSEE593 pKa = 4.43 EE594 pKa = 3.99 EE595 pKa = 4.28 QEE597 pKa = 4.35 TSQVWRR603 pKa = 11.84 RR604 pKa = 11.84 KK605 pKa = 6.85 LQRR608 pKa = 11.84 MGKK611 pKa = 8.88 RR612 pKa = 11.84 LRR614 pKa = 11.84 RR615 pKa = 11.84 EE616 pKa = 3.47 QQYY619 pKa = 10.18 RR620 pKa = 11.84 RR621 pKa = 11.84 RR622 pKa = 11.84 VKK624 pKa = 10.32 QRR626 pKa = 11.84 LLEE629 pKa = 4.03 LSGYY633 pKa = 7.15 QHH635 pKa = 7.68 PSTQFMEE642 pKa = 4.41
Molecular weight: 75.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.677
IPC_protein 10.511
Toseland 10.409
ProMoST 10.189
Dawson 10.584
Bjellqvist 10.335
Wikipedia 10.818
Rodwell 10.73
Grimsley 10.672
Solomon 10.657
Lehninger 10.613
Nozaki 10.423
DTASelect 10.321
Thurlkill 10.452
EMBOSS 10.818
Sillero 10.511
Patrickios 10.292
IPC_peptide 10.657
IPC2_peptide 9.458
IPC2.peptide.svr19 8.518
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
642
642
642
642.0
75.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.517 ± 0.0
1.713 ± 0.0
3.271 ± 0.0
4.829 ± 0.0
4.361 ± 0.0
6.386 ± 0.0
2.336 ± 0.0
5.14 ± 0.0
3.738 ± 0.0
7.321 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.804 ± 0.0
4.673 ± 0.0
8.255 ± 0.0
4.206 ± 0.0
11.526 ± 0.0
5.14 ± 0.0
6.542 ± 0.0
5.607 ± 0.0
3.894 ± 0.0
3.738 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here