Amino acid dipepetide frequency for Lactobacillus phage LR2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.453AlaAla: 4.453 ± 0.663
0.262AlaCys: 0.262 ± 0.163
5.326AlaAsp: 5.326 ± 0.702
4.104AlaGlu: 4.104 ± 0.841
2.27AlaPhe: 2.27 ± 0.409
3.667AlaGly: 3.667 ± 0.482
0.611AlaHis: 0.611 ± 0.254
5.501AlaIle: 5.501 ± 0.838
6.81AlaLys: 6.81 ± 0.816
5.763AlaLeu: 5.763 ± 0.713
2.357AlaMet: 2.357 ± 0.586
5.763AlaAsn: 5.763 ± 0.853
1.222AlaPro: 1.222 ± 0.362
3.667AlaGln: 3.667 ± 0.608
2.445AlaArg: 2.445 ± 0.536
6.199AlaSer: 6.199 ± 1.192
4.715AlaThr: 4.715 ± 0.609
4.278AlaVal: 4.278 ± 0.643
1.135AlaTrp: 1.135 ± 0.338
2.27AlaTyr: 2.27 ± 0.497
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.262CysAsp: 0.262 ± 0.201
0.175CysGlu: 0.175 ± 0.12
0.437CysPhe: 0.437 ± 0.226
0.175CysGly: 0.175 ± 0.133
0.175CysHis: 0.175 ± 0.19
0.0CysIle: 0.0 ± 0.0
0.524CysLys: 0.524 ± 0.255
0.262CysLeu: 0.262 ± 0.16
0.0CysMet: 0.0 ± 0.0
0.262CysAsn: 0.262 ± 0.153
0.175CysPro: 0.175 ± 0.145
0.262CysGln: 0.262 ± 0.163
0.437CysArg: 0.437 ± 0.198
0.262CysSer: 0.262 ± 0.208
0.262CysThr: 0.262 ± 0.169
0.437CysVal: 0.437 ± 0.187
0.087CysTrp: 0.087 ± 0.094
0.262CysTyr: 0.262 ± 0.18
0.0CysXaa: 0.0 ± 0.0
Asp
4.89AspAla: 4.89 ± 0.693
0.262AspCys: 0.262 ± 0.161
5.763AspAsp: 5.763 ± 0.828
3.929AspGlu: 3.929 ± 0.788
3.056AspPhe: 3.056 ± 0.531
5.239AspGly: 5.239 ± 0.734
1.659AspHis: 1.659 ± 0.43
3.667AspIle: 3.667 ± 0.69
6.199AspLys: 6.199 ± 0.809
5.064AspLeu: 5.064 ± 0.689
2.096AspMet: 2.096 ± 0.416
5.064AspAsn: 5.064 ± 0.573
2.619AspPro: 2.619 ± 0.563
3.754AspGln: 3.754 ± 0.669
1.921AspArg: 1.921 ± 0.312
4.278AspSer: 4.278 ± 0.622
3.405AspThr: 3.405 ± 0.69
4.191AspVal: 4.191 ± 0.583
1.222AspTrp: 1.222 ± 0.349
2.707AspTyr: 2.707 ± 0.538
0.0AspXaa: 0.0 ± 0.0
Glu
4.366GluAla: 4.366 ± 0.586
0.175GluCys: 0.175 ± 0.112
2.183GluAsp: 2.183 ± 0.368
2.27GluGlu: 2.27 ± 0.495
1.31GluPhe: 1.31 ± 0.301
1.659GluGly: 1.659 ± 0.399
0.873GluHis: 0.873 ± 0.308
4.016GluIle: 4.016 ± 0.704
4.278GluLys: 4.278 ± 0.584
5.763GluLeu: 5.763 ± 0.853
1.135GluMet: 1.135 ± 0.276
3.318GluAsn: 3.318 ± 0.512
1.746GluPro: 1.746 ± 0.462
3.667GluGln: 3.667 ± 0.563
2.27GluArg: 2.27 ± 0.432
2.969GluSer: 2.969 ± 0.439
2.707GluThr: 2.707 ± 0.588
3.58GluVal: 3.58 ± 0.484
0.524GluTrp: 0.524 ± 0.242
1.834GluTyr: 1.834 ± 0.325
0.0GluXaa: 0.0 ± 0.0
Phe
3.405PheAla: 3.405 ± 0.406
0.087PheCys: 0.087 ± 0.083
2.445PheAsp: 2.445 ± 0.389
2.008PheGlu: 2.008 ± 0.421
1.135PhePhe: 1.135 ± 0.246
2.357PheGly: 2.357 ± 0.462
0.699PheHis: 0.699 ± 0.338
2.707PheIle: 2.707 ± 0.403
2.27PheLys: 2.27 ± 0.442
1.572PheLeu: 1.572 ± 0.416
0.873PheMet: 0.873 ± 0.246
2.619PheAsn: 2.619 ± 0.53
0.786PhePro: 0.786 ± 0.276
1.222PheGln: 1.222 ± 0.264
1.222PheArg: 1.222 ± 0.415
1.746PheSer: 1.746 ± 0.418
2.357PheThr: 2.357 ± 0.446
2.445PheVal: 2.445 ± 0.426
0.611PheTrp: 0.611 ± 0.206
1.484PheTyr: 1.484 ± 0.319
0.0PheXaa: 0.0 ± 0.0
Gly
3.667GlyAla: 3.667 ± 0.741
0.175GlyCys: 0.175 ± 0.111
5.064GlyAsp: 5.064 ± 0.724
2.707GlyGlu: 2.707 ± 0.638
2.619GlyPhe: 2.619 ± 0.391
3.58GlyGly: 3.58 ± 0.75
1.572GlyHis: 1.572 ± 0.355
3.58GlyIle: 3.58 ± 0.507
5.588GlyLys: 5.588 ± 0.734
5.239GlyLeu: 5.239 ± 0.744
0.96GlyMet: 0.96 ± 0.287
3.754GlyAsn: 3.754 ± 0.557
0.96GlyPro: 0.96 ± 0.343
3.143GlyGln: 3.143 ± 0.532
2.707GlyArg: 2.707 ± 0.495
3.842GlySer: 3.842 ± 0.586
5.064GlyThr: 5.064 ± 0.632
3.58GlyVal: 3.58 ± 0.601
1.048GlyTrp: 1.048 ± 0.328
3.231GlyTyr: 3.231 ± 0.585
0.0GlyXaa: 0.0 ± 0.0
His
1.135HisAla: 1.135 ± 0.278
0.087HisCys: 0.087 ± 0.083
1.572HisAsp: 1.572 ± 0.279
0.611HisGlu: 0.611 ± 0.222
1.31HisPhe: 1.31 ± 0.292
1.572HisGly: 1.572 ± 0.431
0.349HisHis: 0.349 ± 0.18
0.786HisIle: 0.786 ± 0.265
0.873HisLys: 0.873 ± 0.33
1.834HisLeu: 1.834 ± 0.497
0.524HisMet: 0.524 ± 0.194
0.786HisAsn: 0.786 ± 0.316
0.873HisPro: 0.873 ± 0.207
0.873HisGln: 0.873 ± 0.261
0.611HisArg: 0.611 ± 0.237
1.659HisSer: 1.659 ± 0.384
0.873HisThr: 0.873 ± 0.303
1.222HisVal: 1.222 ± 0.255
0.524HisTrp: 0.524 ± 0.224
1.048HisTyr: 1.048 ± 0.392
0.0HisXaa: 0.0 ± 0.0
Ile
4.89IleAla: 4.89 ± 0.81
0.262IleCys: 0.262 ± 0.158
4.54IleAsp: 4.54 ± 0.613
3.318IleGlu: 3.318 ± 0.472
1.834IlePhe: 1.834 ± 0.38
3.842IleGly: 3.842 ± 0.57
1.31IleHis: 1.31 ± 0.338
3.493IleIle: 3.493 ± 0.579
6.025IleLys: 6.025 ± 0.759
3.405IleLeu: 3.405 ± 0.525
0.96IleMet: 0.96 ± 0.323
6.549IleAsn: 6.549 ± 0.68
2.096IlePro: 2.096 ± 0.39
3.056IleGln: 3.056 ± 0.487
1.921IleArg: 1.921 ± 0.47
4.278IleSer: 4.278 ± 0.892
4.977IleThr: 4.977 ± 0.928
2.619IleVal: 2.619 ± 0.442
0.349IleTrp: 0.349 ± 0.145
2.183IleTyr: 2.183 ± 0.451
0.0IleXaa: 0.0 ± 0.0
Lys
6.025LysAla: 6.025 ± 0.699
0.524LysCys: 0.524 ± 0.299
6.112LysAsp: 6.112 ± 0.831
4.366LysGlu: 4.366 ± 0.779
2.619LysPhe: 2.619 ± 0.422
4.89LysGly: 4.89 ± 0.905
1.834LysHis: 1.834 ± 0.435
5.064LysIle: 5.064 ± 0.655
5.326LysLys: 5.326 ± 1.109
6.112LysLeu: 6.112 ± 0.717
2.619LysMet: 2.619 ± 0.504
3.754LysAsn: 3.754 ± 0.748
2.619LysPro: 2.619 ± 0.53
5.588LysGln: 5.588 ± 0.647
2.532LysArg: 2.532 ± 0.43
5.588LysSer: 5.588 ± 1.01
5.239LysThr: 5.239 ± 0.898
4.977LysVal: 4.977 ± 0.656
1.048LysTrp: 1.048 ± 0.328
3.318LysTyr: 3.318 ± 0.683
0.0LysXaa: 0.0 ± 0.0
Leu
5.937LeuAla: 5.937 ± 0.929
0.699LeuCys: 0.699 ± 0.305
5.763LeuAsp: 5.763 ± 0.748
2.881LeuGlu: 2.881 ± 0.606
1.921LeuPhe: 1.921 ± 0.476
4.453LeuGly: 4.453 ± 0.751
1.222LeuHis: 1.222 ± 0.319
4.366LeuIle: 4.366 ± 0.724
6.287LeuLys: 6.287 ± 0.768
5.763LeuLeu: 5.763 ± 0.928
2.357LeuMet: 2.357 ± 0.665
6.374LeuAsn: 6.374 ± 0.553
2.619LeuPro: 2.619 ± 0.367
3.842LeuGln: 3.842 ± 0.748
3.318LeuArg: 3.318 ± 0.652
7.16LeuSer: 7.16 ± 0.994
5.064LeuThr: 5.064 ± 0.74
4.191LeuVal: 4.191 ± 0.65
0.611LeuTrp: 0.611 ± 0.251
2.008LeuTyr: 2.008 ± 0.493
0.0LeuXaa: 0.0 ± 0.0
Met
2.794MetAla: 2.794 ± 0.485
0.0MetCys: 0.0 ± 0.0
1.397MetAsp: 1.397 ± 0.332
0.873MetGlu: 0.873 ± 0.33
0.699MetPhe: 0.699 ± 0.241
1.484MetGly: 1.484 ± 0.292
0.96MetHis: 0.96 ± 0.233
1.746MetIle: 1.746 ± 0.399
1.921MetLys: 1.921 ± 0.292
2.445MetLeu: 2.445 ± 0.559
0.699MetMet: 0.699 ± 0.312
1.659MetAsn: 1.659 ± 0.46
0.873MetPro: 0.873 ± 0.311
1.222MetGln: 1.222 ± 0.262
0.873MetArg: 0.873 ± 0.272
1.921MetSer: 1.921 ± 0.435
2.357MetThr: 2.357 ± 0.405
1.31MetVal: 1.31 ± 0.371
0.349MetTrp: 0.349 ± 0.162
0.786MetTyr: 0.786 ± 0.375
0.0MetXaa: 0.0 ± 0.0
Asn
4.977AsnAla: 4.977 ± 0.608
0.175AsnCys: 0.175 ± 0.142
3.929AsnAsp: 3.929 ± 0.38
4.016AsnGlu: 4.016 ± 0.605
1.572AsnPhe: 1.572 ± 0.37
5.675AsnGly: 5.675 ± 0.786
1.572AsnHis: 1.572 ± 0.435
4.453AsnIle: 4.453 ± 1.047
6.199AsnLys: 6.199 ± 0.709
3.754AsnLeu: 3.754 ± 0.734
1.397AsnMet: 1.397 ± 0.39
6.025AsnAsn: 6.025 ± 0.922
2.27AsnPro: 2.27 ± 0.545
4.453AsnGln: 4.453 ± 0.782
2.794AsnArg: 2.794 ± 0.545
4.191AsnSer: 4.191 ± 0.593
4.802AsnThr: 4.802 ± 0.712
3.493AsnVal: 3.493 ± 0.425
1.484AsnTrp: 1.484 ± 0.384
2.008AsnTyr: 2.008 ± 0.476
0.0AsnXaa: 0.0 ± 0.0
Pro
1.834ProAla: 1.834 ± 0.586
0.0ProCys: 0.0 ± 0.0
2.619ProAsp: 2.619 ± 0.504
2.445ProGlu: 2.445 ± 0.706
1.048ProPhe: 1.048 ± 0.317
2.357ProGly: 2.357 ± 0.49
0.524ProHis: 0.524 ± 0.268
2.096ProIle: 2.096 ± 0.415
2.707ProLys: 2.707 ± 0.543
1.659ProLeu: 1.659 ± 0.546
0.786ProMet: 0.786 ± 0.315
2.096ProAsn: 2.096 ± 0.567
1.135ProPro: 1.135 ± 0.559
1.484ProGln: 1.484 ± 0.304
1.222ProArg: 1.222 ± 0.266
2.008ProSer: 2.008 ± 0.506
1.834ProThr: 1.834 ± 0.426
1.31ProVal: 1.31 ± 0.407
0.262ProTrp: 0.262 ± 0.169
1.048ProTyr: 1.048 ± 0.292
0.0ProXaa: 0.0 ± 0.0
Gln
3.929GlnAla: 3.929 ± 0.667
0.087GlnCys: 0.087 ± 0.098
3.405GlnAsp: 3.405 ± 0.578
2.532GlnGlu: 2.532 ± 0.399
1.834GlnPhe: 1.834 ± 0.328
3.58GlnGly: 3.58 ± 0.531
1.048GlnHis: 1.048 ± 0.348
3.58GlnIle: 3.58 ± 0.579
3.929GlnLys: 3.929 ± 0.642
5.151GlnLeu: 5.151 ± 0.727
1.659GlnMet: 1.659 ± 0.515
2.707GlnAsn: 2.707 ± 0.511
1.746GlnPro: 1.746 ± 0.512
4.191GlnGln: 4.191 ± 0.631
2.096GlnArg: 2.096 ± 0.483
3.754GlnSer: 3.754 ± 0.563
4.453GlnThr: 4.453 ± 0.575
4.453GlnVal: 4.453 ± 0.574
0.699GlnTrp: 0.699 ± 0.359
2.707GlnTyr: 2.707 ± 0.533
0.0GlnXaa: 0.0 ± 0.0
Arg
2.357ArgAla: 2.357 ± 0.454
0.175ArgCys: 0.175 ± 0.129
2.619ArgAsp: 2.619 ± 0.603
2.707ArgGlu: 2.707 ± 0.639
1.746ArgPhe: 1.746 ± 0.401
1.31ArgGly: 1.31 ± 0.327
0.786ArgHis: 0.786 ± 0.302
2.357ArgIle: 2.357 ± 0.5
3.056ArgLys: 3.056 ± 0.667
3.493ArgLeu: 3.493 ± 0.542
0.96ArgMet: 0.96 ± 0.316
2.008ArgAsn: 2.008 ± 0.368
0.96ArgPro: 0.96 ± 0.25
2.794ArgGln: 2.794 ± 0.452
1.659ArgArg: 1.659 ± 0.442
2.532ArgSer: 2.532 ± 0.49
1.746ArgThr: 1.746 ± 0.413
1.746ArgVal: 1.746 ± 0.376
0.699ArgTrp: 0.699 ± 0.192
1.397ArgTyr: 1.397 ± 0.437
0.0ArgXaa: 0.0 ± 0.0
Ser
5.588SerAla: 5.588 ± 1.076
0.175SerCys: 0.175 ± 0.117
4.802SerAsp: 4.802 ± 0.939
3.143SerGlu: 3.143 ± 0.522
3.318SerPhe: 3.318 ± 0.547
4.715SerGly: 4.715 ± 0.768
1.135SerHis: 1.135 ± 0.28
4.628SerIle: 4.628 ± 0.684
6.898SerLys: 6.898 ± 0.682
4.89SerLeu: 4.89 ± 0.846
2.357SerMet: 2.357 ± 0.346
5.326SerAsn: 5.326 ± 0.506
1.572SerPro: 1.572 ± 0.403
4.016SerGln: 4.016 ± 0.668
2.357SerArg: 2.357 ± 0.457
6.025SerSer: 6.025 ± 0.929
4.104SerThr: 4.104 ± 0.695
3.231SerVal: 3.231 ± 0.433
0.786SerTrp: 0.786 ± 0.354
2.27SerTyr: 2.27 ± 0.448
0.0SerXaa: 0.0 ± 0.0
Thr
4.89ThrAla: 4.89 ± 0.67
0.262ThrCys: 0.262 ± 0.206
5.239ThrAsp: 5.239 ± 0.741
3.056ThrGlu: 3.056 ± 0.451
1.397ThrPhe: 1.397 ± 0.348
4.89ThrGly: 4.89 ± 0.453
1.048ThrHis: 1.048 ± 0.301
3.842ThrIle: 3.842 ± 0.716
3.318ThrLys: 3.318 ± 0.592
5.239ThrLeu: 5.239 ± 0.68
1.484ThrMet: 1.484 ± 0.34
3.667ThrAsn: 3.667 ± 0.51
3.318ThrPro: 3.318 ± 0.889
2.794ThrGln: 2.794 ± 0.418
2.357ThrArg: 2.357 ± 0.349
4.89ThrSer: 4.89 ± 0.675
3.667ThrThr: 3.667 ± 0.739
4.715ThrVal: 4.715 ± 0.823
1.048ThrTrp: 1.048 ± 0.375
3.143ThrTyr: 3.143 ± 0.725
0.0ThrXaa: 0.0 ± 0.0
Val
3.667ValAla: 3.667 ± 0.659
0.262ValCys: 0.262 ± 0.147
4.366ValAsp: 4.366 ± 0.608
2.969ValGlu: 2.969 ± 0.531
1.659ValPhe: 1.659 ± 0.377
3.929ValGly: 3.929 ± 0.61
0.611ValHis: 0.611 ± 0.224
3.929ValIle: 3.929 ± 0.652
4.977ValLys: 4.977 ± 0.404
4.366ValLeu: 4.366 ± 0.573
1.397ValMet: 1.397 ± 0.348
4.54ValAsn: 4.54 ± 0.908
2.357ValPro: 2.357 ± 0.38
3.842ValGln: 3.842 ± 0.777
1.484ValArg: 1.484 ± 0.408
4.278ValSer: 4.278 ± 0.731
4.191ValThr: 4.191 ± 0.933
2.532ValVal: 2.532 ± 0.456
0.262ValTrp: 0.262 ± 0.134
2.619ValTyr: 2.619 ± 0.455
0.0ValXaa: 0.0 ± 0.0
Trp
0.699TrpAla: 0.699 ± 0.292
0.087TrpCys: 0.087 ± 0.097
0.437TrpAsp: 0.437 ± 0.161
0.524TrpGlu: 0.524 ± 0.179
0.611TrpPhe: 0.611 ± 0.221
0.96TrpGly: 0.96 ± 0.401
0.349TrpHis: 0.349 ± 0.176
0.437TrpIle: 0.437 ± 0.237
1.048TrpLys: 1.048 ± 0.383
1.048TrpLeu: 1.048 ± 0.245
0.524TrpMet: 0.524 ± 0.229
1.048TrpAsn: 1.048 ± 0.321
0.087TrpPro: 0.087 ± 0.094
1.31TrpGln: 1.31 ± 0.381
0.699TrpArg: 0.699 ± 0.223
0.873TrpSer: 0.873 ± 0.261
0.786TrpThr: 0.786 ± 0.408
0.873TrpVal: 0.873 ± 0.295
0.262TrpTrp: 0.262 ± 0.133
0.873TrpTyr: 0.873 ± 0.333
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.405TyrAla: 3.405 ± 0.636
0.699TyrCys: 0.699 ± 0.337
3.143TyrAsp: 3.143 ± 0.554
2.008TyrGlu: 2.008 ± 0.35
1.659TyrPhe: 1.659 ± 0.459
1.746TyrGly: 1.746 ± 0.371
0.786TyrHis: 0.786 ± 0.253
1.659TyrIle: 1.659 ± 0.386
1.921TyrLys: 1.921 ± 0.427
4.016TyrLeu: 4.016 ± 0.683
1.135TyrMet: 1.135 ± 0.277
1.921TyrAsn: 1.921 ± 0.427
0.437TyrPro: 0.437 ± 0.2
2.357TyrGln: 2.357 ± 0.553
2.183TyrArg: 2.183 ± 0.495
2.881TyrSer: 2.881 ± 0.625
1.659TyrThr: 1.659 ± 0.492
3.056TyrVal: 3.056 ± 0.479
0.524TyrTrp: 0.524 ± 0.175
1.659TyrTyr: 1.659 ± 0.598
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 41 proteins (11454 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski