Culex-associated Tombus-like virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; unclassified Tombusviridae

Average proteome isoelectric point is 8.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346M220|A0A346M220_9TOMB RNA-directed RNA polymerase OS=Culex-associated Tombus-like virus OX=2304516 PE=4 SV=1
MM1 pKa = 7.54SIYY4 pKa = 10.6HH5 pKa = 6.36SMPDD9 pKa = 3.4LTRR12 pKa = 11.84PPPEE16 pKa = 5.28LGTIRR21 pKa = 11.84PIQDD25 pKa = 2.11GMMPRR30 pKa = 11.84TRR32 pKa = 11.84TQPRR36 pKa = 11.84PTGFISNLTRR46 pKa = 11.84GIQNAANRR54 pKa = 11.84AYY56 pKa = 10.69DD57 pKa = 3.38WMIPPEE63 pKa = 4.26LTDD66 pKa = 3.44AEE68 pKa = 4.43NIEE71 pKa = 4.3RR72 pKa = 11.84ADD74 pKa = 3.64EE75 pKa = 4.43VVQAWTRR82 pKa = 11.84RR83 pKa = 11.84QEE85 pKa = 4.15TEE87 pKa = 3.31IKK89 pKa = 9.78ARR91 pKa = 11.84RR92 pKa = 11.84MDD94 pKa = 3.42IDD96 pKa = 3.54NEE98 pKa = 4.42TTRR101 pKa = 11.84RR102 pKa = 11.84TAEE105 pKa = 3.75IMAQPLEE112 pKa = 4.44EE113 pKa = 4.72GLLAYY118 pKa = 10.9LNGQTRR124 pKa = 11.84GKK126 pKa = 9.75ILNKK130 pKa = 10.38DD131 pKa = 3.53YY132 pKa = 11.44VPYY135 pKa = 10.43LVKK138 pKa = 10.87LSNAYY143 pKa = 9.38FAEE146 pKa = 4.46VGQPDD151 pKa = 4.09HH152 pKa = 6.94ILRR155 pKa = 11.84EE156 pKa = 4.07KK157 pKa = 11.02VMQDD161 pKa = 3.32VIKK164 pKa = 10.64KK165 pKa = 9.9HH166 pKa = 6.25LDD168 pKa = 3.12FRR170 pKa = 11.84LKK172 pKa = 9.77DD173 pKa = 3.71TPFLTSRR180 pKa = 11.84TDD182 pKa = 2.79IMHH185 pKa = 6.69RR186 pKa = 11.84ASYY189 pKa = 10.75INALNQGKK197 pKa = 10.33RR198 pKa = 11.84ITYY201 pKa = 6.7PFWYY205 pKa = 9.79FGRR208 pKa = 11.84PKK210 pKa = 10.55VEE212 pKa = 5.1TIQEE216 pKa = 3.81WSEE219 pKa = 4.24NEE221 pKa = 3.92SGPYY225 pKa = 9.89DD226 pKa = 3.52SPRR229 pKa = 11.84PYY231 pKa = 10.24SYY233 pKa = 11.4SRR235 pKa = 11.84IILTTMGWVAVGWTAKK251 pKa = 10.6YY252 pKa = 10.33LISRR256 pKa = 11.84LYY258 pKa = 11.34SNLTSGTTTQLAVKK272 pKa = 9.46QLPLPSGTGIFRR284 pKa = 11.84KK285 pKa = 9.42FFPSTTPQVSTNSTDD300 pKa = 3.15TSGFTAYY307 pKa = 9.49ILEE310 pKa = 4.21YY311 pKa = 11.04GPIKK315 pKa = 10.83SMLVACSRR323 pKa = 11.84PVKK326 pKa = 10.37EE327 pKa = 5.07FATKK331 pKa = 10.65LPP333 pKa = 3.86

Molecular weight: 38.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346M220|A0A346M220_9TOMB RNA-directed RNA polymerase OS=Culex-associated Tombus-like virus OX=2304516 PE=4 SV=1
MM1 pKa = 6.49VGKK4 pKa = 10.58RR5 pKa = 11.84IGPLRR10 pKa = 11.84FTKK13 pKa = 9.92TVQLLQNYY21 pKa = 7.38PDD23 pKa = 3.52NYY25 pKa = 10.63GLGRR29 pKa = 11.84CRR31 pKa = 11.84MDD33 pKa = 3.03RR34 pKa = 11.84QIPYY38 pKa = 9.7FPSLLKK44 pKa = 10.23PDD46 pKa = 3.03EE47 pKa = 4.18WYY49 pKa = 10.41YY50 pKa = 11.42YY51 pKa = 9.19PVSSEE56 pKa = 3.73TTAAALRR63 pKa = 11.84NRR65 pKa = 11.84HH66 pKa = 5.75LPEE69 pKa = 4.42ILPEE73 pKa = 3.83YY74 pKa = 10.32DD75 pKa = 2.84AAGINKK81 pKa = 9.63LYY83 pKa = 10.57RR84 pKa = 11.84HH85 pKa = 5.41IRR87 pKa = 11.84VYY89 pKa = 10.27RR90 pKa = 11.84VHH92 pKa = 6.32PRR94 pKa = 11.84VWTHH98 pKa = 4.77QEE100 pKa = 4.14YY101 pKa = 10.13VGSMQPPSKK110 pKa = 10.26RR111 pKa = 11.84ICYY114 pKa = 9.1QAALDD119 pKa = 3.93DD120 pKa = 4.23YY121 pKa = 11.14YY122 pKa = 11.53RR123 pKa = 11.84DD124 pKa = 3.77GLISTTLQPFTKK136 pKa = 9.28IEE138 pKa = 3.81KK139 pKa = 9.3MKK141 pKa = 10.17NSKK144 pKa = 10.29YY145 pKa = 9.98KK146 pKa = 10.62APRR149 pKa = 11.84LIQARR154 pKa = 11.84KK155 pKa = 8.43PIFNIMYY162 pKa = 9.45GRR164 pKa = 11.84YY165 pKa = 9.03VKK167 pKa = 10.59ALEE170 pKa = 4.92HH171 pKa = 6.73ILFKK175 pKa = 10.27HH176 pKa = 6.17HH177 pKa = 7.13RR178 pKa = 11.84LTYY181 pKa = 10.48HH182 pKa = 6.56FGKK185 pKa = 8.9GTNEE189 pKa = 3.96NIADD193 pKa = 4.43KK194 pKa = 10.31IYY196 pKa = 10.93KK197 pKa = 10.36LSQKK201 pKa = 7.73WQWKK205 pKa = 7.85TEE207 pKa = 4.11GDD209 pKa = 3.63HH210 pKa = 6.09KK211 pKa = 10.01TFDD214 pKa = 3.08AHH216 pKa = 5.38VTVEE220 pKa = 4.11HH221 pKa = 6.75KK222 pKa = 10.86KK223 pKa = 10.7LFNKK227 pKa = 8.27FLCACFPQNVKK238 pKa = 10.13EE239 pKa = 4.2IKK241 pKa = 10.61NIGKK245 pKa = 9.77RR246 pKa = 11.84LLTAKK251 pKa = 10.13CRR253 pKa = 11.84TRR255 pKa = 11.84SGEE258 pKa = 3.7TWTVKK263 pKa = 9.07GTQQSGEE270 pKa = 4.03VDD272 pKa = 3.29TSISNTIINIAILKK286 pKa = 9.89EE287 pKa = 4.0LMHH290 pKa = 6.05QLGIKK295 pKa = 10.63GEE297 pKa = 4.17VLANGDD303 pKa = 4.11DD304 pKa = 5.18FILFTNKK311 pKa = 9.75PVDD314 pKa = 3.31IEE316 pKa = 4.11KK317 pKa = 10.82SKK319 pKa = 11.14EE320 pKa = 3.61ILKK323 pKa = 8.99TMNMEE328 pKa = 4.2TEE330 pKa = 4.11MHH332 pKa = 6.54KK333 pKa = 9.42STRR336 pKa = 11.84NIHH339 pKa = 4.95TVEE342 pKa = 4.61FCANKK347 pKa = 10.12LAFSSTGQHH356 pKa = 6.09VLFKK360 pKa = 10.62DD361 pKa = 2.93IDD363 pKa = 4.1RR364 pKa = 11.84IYY366 pKa = 11.33SKK368 pKa = 11.13FGMTNVQVDD377 pKa = 3.99YY378 pKa = 11.13YY379 pKa = 9.69RR380 pKa = 11.84TYY382 pKa = 11.57LMEE385 pKa = 4.68CAHH388 pKa = 6.62GNWKK392 pKa = 8.82MMKK395 pKa = 8.73DD396 pKa = 3.46TPIGQEE402 pKa = 3.84FKK404 pKa = 10.6RR405 pKa = 11.84IYY407 pKa = 10.65YY408 pKa = 9.33YY409 pKa = 10.15ILYY412 pKa = 10.37LEE414 pKa = 4.49EE415 pKa = 4.22QLTGQDD421 pKa = 2.93QRR423 pKa = 11.84QVQRR427 pKa = 11.84QHH429 pKa = 5.84MKK431 pKa = 10.25LQYY434 pKa = 10.39KK435 pKa = 9.69YY436 pKa = 10.9LEE438 pKa = 4.34KK439 pKa = 10.23EE440 pKa = 3.82YY441 pKa = 11.1QRR443 pKa = 11.84IIKK446 pKa = 9.11EE447 pKa = 4.03NKK449 pKa = 8.43DD450 pKa = 3.28AKK452 pKa = 10.57EE453 pKa = 4.02NTSTEE458 pKa = 4.21LTVSMMEE465 pKa = 4.7AYY467 pKa = 10.29DD468 pKa = 3.69EE469 pKa = 4.81ALDD472 pKa = 3.96LKK474 pKa = 10.83KK475 pKa = 10.26YY476 pKa = 8.1TSKK479 pKa = 11.13LINRR483 pKa = 11.84IKK485 pKa = 10.62NIYY488 pKa = 8.48TNYY491 pKa = 10.09PYY493 pKa = 9.97MGSIGLKK500 pKa = 8.17YY501 pKa = 10.11LAYY504 pKa = 10.28NSIMTISHH512 pKa = 6.35DD513 pKa = 3.64TKK515 pKa = 9.86QSEE518 pKa = 4.11RR519 pKa = 11.84HH520 pKa = 6.07KK521 pKa = 10.85YY522 pKa = 8.83TDD524 pKa = 3.24TDD526 pKa = 3.62EE527 pKa = 4.91LKK529 pKa = 10.91QSIQPLLHH537 pKa = 6.04QAFKK541 pKa = 10.52TKK543 pKa = 10.4NNRR546 pKa = 11.84HH547 pKa = 5.74IYY549 pKa = 9.83KK550 pKa = 10.29KK551 pKa = 9.84IRR553 pKa = 11.84RR554 pKa = 11.84LTGLKK559 pKa = 10.19

Molecular weight: 66.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

892

333

559

446.0

52.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.933 ± 0.581

0.897 ± 0.323

4.484 ± 0.011

5.83 ± 0.067

3.251 ± 0.028

4.933 ± 0.256

2.803 ± 0.868

7.287 ± 0.206

8.408 ± 1.952

8.184 ± 0.204

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.251 ± 0.028

4.596 ± 0.375

5.045 ± 1.497

4.821 ± 0.334

6.502 ± 0.707

5.157 ± 0.623

8.296 ± 0.874

3.924 ± 0.152

1.345 ± 0.247

6.054 ± 0.677
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski