Amino acid dipepetide frequency for Mycobacterium phage Ringer

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.471AlaAla: 12.471 ± 1.327
0.683AlaCys: 0.683 ± 0.203
6.887AlaAsp: 6.887 ± 0.723
5.894AlaGlu: 5.894 ± 0.759
3.04AlaPhe: 3.04 ± 0.394
7.073AlaGly: 7.073 ± 0.881
1.241AlaHis: 1.241 ± 0.302
4.591AlaIle: 4.591 ± 0.652
3.661AlaLys: 3.661 ± 0.556
8.935AlaLeu: 8.935 ± 0.959
2.42AlaMet: 2.42 ± 0.469
2.482AlaAsn: 2.482 ± 0.317
5.274AlaPro: 5.274 ± 0.666
3.102AlaGln: 3.102 ± 0.511
6.018AlaArg: 6.018 ± 0.5
4.653AlaSer: 4.653 ± 0.525
6.267AlaThr: 6.267 ± 0.665
8.686AlaVal: 8.686 ± 0.754
2.358AlaTrp: 2.358 ± 0.338
3.102AlaTyr: 3.102 ± 0.441
0.0AlaXaa: 0.0 ± 0.0
Cys
0.807CysAla: 0.807 ± 0.226
0.0CysCys: 0.0 ± 0.0
0.496CysAsp: 0.496 ± 0.165
0.683CysGlu: 0.683 ± 0.193
0.062CysPhe: 0.062 ± 0.061
0.683CysGly: 0.683 ± 0.21
0.248CysHis: 0.248 ± 0.111
0.248CysIle: 0.248 ± 0.143
0.248CysLys: 0.248 ± 0.114
0.434CysLeu: 0.434 ± 0.167
0.186CysMet: 0.186 ± 0.105
0.31CysAsn: 0.31 ± 0.137
0.248CysPro: 0.248 ± 0.116
0.186CysGln: 0.186 ± 0.089
0.496CysArg: 0.496 ± 0.146
0.248CysSer: 0.248 ± 0.117
0.248CysThr: 0.248 ± 0.121
0.31CysVal: 0.31 ± 0.126
0.186CysTrp: 0.186 ± 0.107
0.124CysTyr: 0.124 ± 0.071
0.0CysXaa: 0.0 ± 0.0
Asp
6.143AspAla: 6.143 ± 0.638
0.496AspCys: 0.496 ± 0.149
4.343AspAsp: 4.343 ± 0.47
3.785AspGlu: 3.785 ± 0.521
2.296AspPhe: 2.296 ± 0.314
6.143AspGly: 6.143 ± 0.631
1.117AspHis: 1.117 ± 0.288
2.854AspIle: 2.854 ± 0.417
2.358AspLys: 2.358 ± 0.442
6.701AspLeu: 6.701 ± 0.74
1.055AspMet: 1.055 ± 0.203
1.799AspAsn: 1.799 ± 0.363
4.653AspPro: 4.653 ± 0.78
1.799AspGln: 1.799 ± 0.473
3.785AspArg: 3.785 ± 0.389
3.164AspSer: 3.164 ± 0.408
3.909AspThr: 3.909 ± 0.417
3.909AspVal: 3.909 ± 0.513
1.551AspTrp: 1.551 ± 0.327
2.358AspTyr: 2.358 ± 0.331
0.0AspXaa: 0.0 ± 0.0
Glu
6.205GluAla: 6.205 ± 0.617
0.372GluCys: 0.372 ± 0.141
4.591GluAsp: 4.591 ± 0.57
5.336GluGlu: 5.336 ± 0.706
2.234GluPhe: 2.234 ± 0.347
3.847GluGly: 3.847 ± 0.496
1.737GluHis: 1.737 ± 0.345
3.723GluIle: 3.723 ± 0.5
2.978GluLys: 2.978 ± 0.457
7.135GluLeu: 7.135 ± 0.564
1.551GluMet: 1.551 ± 0.313
1.613GluAsn: 1.613 ± 0.354
2.482GluPro: 2.482 ± 0.433
2.358GluGln: 2.358 ± 0.404
3.661GluArg: 3.661 ± 0.519
3.723GluSer: 3.723 ± 0.5
3.413GluThr: 3.413 ± 0.564
5.894GluVal: 5.894 ± 0.623
1.613GluTrp: 1.613 ± 0.29
2.42GluTyr: 2.42 ± 0.414
0.0GluXaa: 0.0 ± 0.0
Phe
2.296PheAla: 2.296 ± 0.334
0.372PheCys: 0.372 ± 0.153
2.73PheAsp: 2.73 ± 0.332
2.234PheGlu: 2.234 ± 0.301
0.558PhePhe: 0.558 ± 0.182
3.971PheGly: 3.971 ± 0.57
0.745PheHis: 0.745 ± 0.212
1.365PheIle: 1.365 ± 0.266
0.993PheLys: 0.993 ± 0.259
2.606PheLeu: 2.606 ± 0.621
0.558PheMet: 0.558 ± 0.196
1.241PheAsn: 1.241 ± 0.243
1.613PhePro: 1.613 ± 0.32
1.055PheGln: 1.055 ± 0.173
2.048PheArg: 2.048 ± 0.364
1.675PheSer: 1.675 ± 0.264
2.11PheThr: 2.11 ± 0.439
2.172PheVal: 2.172 ± 0.386
0.62PheTrp: 0.62 ± 0.181
0.931PheTyr: 0.931 ± 0.224
0.0PheXaa: 0.0 ± 0.0
Gly
6.763GlyAla: 6.763 ± 0.863
0.558GlyCys: 0.558 ± 0.189
5.77GlyAsp: 5.77 ± 0.537
4.964GlyGlu: 4.964 ± 0.553
2.916GlyPhe: 2.916 ± 0.506
7.942GlyGly: 7.942 ± 1.696
1.985GlyHis: 1.985 ± 0.409
4.84GlyIle: 4.84 ± 0.788
3.661GlyLys: 3.661 ± 0.49
7.135GlyLeu: 7.135 ± 0.652
2.048GlyMet: 2.048 ± 0.334
3.35GlyAsn: 3.35 ± 0.486
3.785GlyPro: 3.785 ± 0.667
2.544GlyGln: 2.544 ± 0.301
5.212GlyArg: 5.212 ± 0.528
5.77GlySer: 5.77 ± 0.835
5.584GlyThr: 5.584 ± 0.699
5.336GlyVal: 5.336 ± 0.538
2.978GlyTrp: 2.978 ± 0.453
2.916GlyTyr: 2.916 ± 0.446
0.0GlyXaa: 0.0 ± 0.0
His
1.737HisAla: 1.737 ± 0.336
0.124HisCys: 0.124 ± 0.116
0.807HisAsp: 0.807 ± 0.206
1.489HisGlu: 1.489 ± 0.293
0.683HisPhe: 0.683 ± 0.188
2.048HisGly: 2.048 ± 0.381
0.931HisHis: 0.931 ± 0.272
1.117HisIle: 1.117 ± 0.233
1.055HisLys: 1.055 ± 0.3
1.551HisLeu: 1.551 ± 0.342
0.124HisMet: 0.124 ± 0.087
0.186HisAsn: 0.186 ± 0.103
1.179HisPro: 1.179 ± 0.252
1.117HisGln: 1.117 ± 0.259
1.427HisArg: 1.427 ± 0.298
0.993HisSer: 0.993 ± 0.235
0.993HisThr: 0.993 ± 0.226
1.737HisVal: 1.737 ± 0.35
0.434HisTrp: 0.434 ± 0.151
0.683HisTyr: 0.683 ± 0.231
0.0HisXaa: 0.0 ± 0.0
Ile
6.081IleAla: 6.081 ± 0.605
0.31IleCys: 0.31 ± 0.13
2.916IleAsp: 2.916 ± 0.391
3.723IleGlu: 3.723 ± 0.434
0.931IlePhe: 0.931 ± 0.244
3.847IleGly: 3.847 ± 0.397
0.869IleHis: 0.869 ± 0.193
1.613IleIle: 1.613 ± 0.301
1.737IleLys: 1.737 ± 0.327
3.475IleLeu: 3.475 ± 0.372
0.807IleMet: 0.807 ± 0.188
1.923IleAsn: 1.923 ± 0.313
3.102IlePro: 3.102 ± 0.391
1.675IleGln: 1.675 ± 0.358
3.04IleArg: 3.04 ± 0.486
3.537IleSer: 3.537 ± 0.423
3.599IleThr: 3.599 ± 0.427
3.102IleVal: 3.102 ± 0.454
0.807IleTrp: 0.807 ± 0.189
1.737IleTyr: 1.737 ± 0.305
0.0IleXaa: 0.0 ± 0.0
Lys
3.909LysAla: 3.909 ± 0.513
0.186LysCys: 0.186 ± 0.106
2.606LysAsp: 2.606 ± 0.408
2.048LysGlu: 2.048 ± 0.341
1.303LysPhe: 1.303 ± 0.297
2.854LysGly: 2.854 ± 0.407
1.179LysHis: 1.179 ± 0.333
1.923LysIle: 1.923 ± 0.419
1.923LysLys: 1.923 ± 0.417
3.226LysLeu: 3.226 ± 0.432
0.993LysMet: 0.993 ± 0.21
1.861LysAsn: 1.861 ± 0.348
2.296LysPro: 2.296 ± 0.412
1.427LysGln: 1.427 ± 0.286
2.792LysArg: 2.792 ± 0.521
2.482LysSer: 2.482 ± 0.411
2.172LysThr: 2.172 ± 0.358
3.785LysVal: 3.785 ± 0.431
0.869LysTrp: 0.869 ± 0.234
0.993LysTyr: 0.993 ± 0.278
0.0LysXaa: 0.0 ± 0.0
Leu
9.307LeuAla: 9.307 ± 1.2
0.31LeuCys: 0.31 ± 0.119
5.956LeuAsp: 5.956 ± 0.547
5.336LeuGlu: 5.336 ± 0.632
2.358LeuPhe: 2.358 ± 0.443
7.011LeuGly: 7.011 ± 0.683
1.365LeuHis: 1.365 ± 0.329
4.033LeuIle: 4.033 ± 0.469
3.785LeuLys: 3.785 ± 0.492
6.391LeuLeu: 6.391 ± 0.646
1.551LeuMet: 1.551 ± 0.283
2.792LeuAsn: 2.792 ± 0.392
5.584LeuPro: 5.584 ± 0.697
2.482LeuGln: 2.482 ± 0.427
5.832LeuArg: 5.832 ± 0.538
5.894LeuSer: 5.894 ± 0.67
6.639LeuThr: 6.639 ± 0.823
4.902LeuVal: 4.902 ± 0.577
0.931LeuTrp: 0.931 ± 0.29
2.234LeuTyr: 2.234 ± 0.42
0.0LeuXaa: 0.0 ± 0.0
Met
2.172MetAla: 2.172 ± 0.324
0.0MetCys: 0.0 ± 0.0
0.993MetAsp: 0.993 ± 0.244
1.675MetGlu: 1.675 ± 0.323
0.683MetPhe: 0.683 ± 0.18
1.551MetGly: 1.551 ± 0.254
0.434MetHis: 0.434 ± 0.217
0.31MetIle: 0.31 ± 0.116
1.427MetLys: 1.427 ± 0.269
0.993MetLeu: 0.993 ± 0.242
0.124MetMet: 0.124 ± 0.081
0.807MetAsn: 0.807 ± 0.193
1.241MetPro: 1.241 ± 0.268
0.62MetGln: 0.62 ± 0.225
1.303MetArg: 1.303 ± 0.237
2.172MetSer: 2.172 ± 0.434
2.296MetThr: 2.296 ± 0.32
1.179MetVal: 1.179 ± 0.313
0.248MetTrp: 0.248 ± 0.127
0.31MetTyr: 0.31 ± 0.122
0.0MetXaa: 0.0 ± 0.0
Asn
3.35AsnAla: 3.35 ± 0.488
0.0AsnCys: 0.0 ± 0.0
2.11AsnAsp: 2.11 ± 0.427
2.048AsnGlu: 2.048 ± 0.364
0.807AsnPhe: 0.807 ± 0.238
3.599AsnGly: 3.599 ± 0.505
0.745AsnHis: 0.745 ± 0.198
1.365AsnIle: 1.365 ± 0.241
0.62AsnLys: 0.62 ± 0.196
2.11AsnLeu: 2.11 ± 0.321
0.807AsnMet: 0.807 ± 0.161
0.993AsnAsn: 0.993 ± 0.268
2.668AsnPro: 2.668 ± 0.381
1.055AsnGln: 1.055 ± 0.248
1.675AsnArg: 1.675 ± 0.323
1.737AsnSer: 1.737 ± 0.398
1.799AsnThr: 1.799 ± 0.342
2.606AsnVal: 2.606 ± 0.409
0.683AsnTrp: 0.683 ± 0.173
1.303AsnTyr: 1.303 ± 0.3
0.0AsnXaa: 0.0 ± 0.0
Pro
5.398ProAla: 5.398 ± 0.653
0.248ProCys: 0.248 ± 0.116
4.219ProAsp: 4.219 ± 0.499
4.033ProGlu: 4.033 ± 0.539
2.048ProPhe: 2.048 ± 0.372
5.088ProGly: 5.088 ± 0.638
0.683ProHis: 0.683 ± 0.207
2.482ProIle: 2.482 ± 0.395
1.799ProLys: 1.799 ± 0.301
4.219ProLeu: 4.219 ± 0.518
0.869ProMet: 0.869 ± 0.276
1.613ProAsn: 1.613 ± 0.274
2.978ProPro: 2.978 ± 0.505
1.613ProGln: 1.613 ± 0.35
2.606ProArg: 2.606 ± 0.474
3.909ProSer: 3.909 ± 0.469
3.909ProThr: 3.909 ± 0.588
3.847ProVal: 3.847 ± 0.567
0.807ProTrp: 0.807 ± 0.301
1.737ProTyr: 1.737 ± 0.355
0.0ProXaa: 0.0 ± 0.0
Gln
3.102GlnAla: 3.102 ± 0.681
0.124GlnCys: 0.124 ± 0.088
1.241GlnAsp: 1.241 ± 0.359
1.737GlnGlu: 1.737 ± 0.314
1.241GlnPhe: 1.241 ± 0.37
2.73GlnGly: 2.73 ± 0.39
0.496GlnHis: 0.496 ± 0.16
2.73GlnIle: 2.73 ± 0.479
1.055GlnLys: 1.055 ± 0.231
3.971GlnLeu: 3.971 ± 0.653
0.931GlnMet: 0.931 ± 0.203
0.62GlnAsn: 0.62 ± 0.172
1.675GlnPro: 1.675 ± 0.282
1.923GlnGln: 1.923 ± 0.456
1.861GlnArg: 1.861 ± 0.325
2.11GlnSer: 2.11 ± 0.295
1.799GlnThr: 1.799 ± 0.355
2.42GlnVal: 2.42 ± 0.331
0.683GlnTrp: 0.683 ± 0.173
0.62GlnTyr: 0.62 ± 0.165
0.0GlnXaa: 0.0 ± 0.0
Arg
4.964ArgAla: 4.964 ± 0.564
0.745ArgCys: 0.745 ± 0.22
2.854ArgAsp: 2.854 ± 0.394
4.964ArgGlu: 4.964 ± 0.627
2.42ArgPhe: 2.42 ± 0.412
5.15ArgGly: 5.15 ± 0.564
1.303ArgHis: 1.303 ± 0.33
3.04ArgIle: 3.04 ± 0.542
3.164ArgLys: 3.164 ± 0.55
5.212ArgLeu: 5.212 ± 0.633
1.737ArgMet: 1.737 ± 0.323
2.296ArgAsn: 2.296 ± 0.44
2.11ArgPro: 2.11 ± 0.342
1.737ArgGln: 1.737 ± 0.293
4.778ArgArg: 4.778 ± 0.672
3.847ArgSer: 3.847 ± 0.493
3.102ArgThr: 3.102 ± 0.546
5.088ArgVal: 5.088 ± 0.584
1.303ArgTrp: 1.303 ± 0.268
1.737ArgTyr: 1.737 ± 0.283
0.0ArgXaa: 0.0 ± 0.0
Ser
5.77SerAla: 5.77 ± 0.635
0.745SerCys: 0.745 ± 0.213
3.164SerAsp: 3.164 ± 0.457
3.785SerGlu: 3.785 ± 0.499
2.048SerPhe: 2.048 ± 0.385
6.391SerGly: 6.391 ± 0.68
1.551SerHis: 1.551 ± 0.307
2.482SerIle: 2.482 ± 0.385
2.42SerLys: 2.42 ± 0.387
5.15SerLeu: 5.15 ± 0.59
1.489SerMet: 1.489 ± 0.26
1.985SerAsn: 1.985 ± 0.338
3.537SerPro: 3.537 ± 0.481
2.296SerGln: 2.296 ± 0.321
2.854SerArg: 2.854 ± 0.366
2.73SerSer: 2.73 ± 0.516
3.599SerThr: 3.599 ± 0.561
3.723SerVal: 3.723 ± 0.432
1.427SerTrp: 1.427 ± 0.333
1.613SerTyr: 1.613 ± 0.314
0.0SerXaa: 0.0 ± 0.0
Thr
6.949ThrAla: 6.949 ± 1.0
0.248ThrCys: 0.248 ± 0.13
4.033ThrAsp: 4.033 ± 0.596
4.467ThrGlu: 4.467 ± 0.634
2.358ThrPhe: 2.358 ± 0.412
6.763ThrGly: 6.763 ± 0.639
1.241ThrHis: 1.241 ± 0.309
3.102ThrIle: 3.102 ± 0.544
2.73ThrLys: 2.73 ± 0.344
5.956ThrLeu: 5.956 ± 0.657
0.807ThrMet: 0.807 ± 0.224
1.613ThrAsn: 1.613 ± 0.357
4.095ThrPro: 4.095 ± 0.482
1.737ThrGln: 1.737 ± 0.416
3.226ThrArg: 3.226 ± 0.472
3.537ThrSer: 3.537 ± 0.581
4.405ThrThr: 4.405 ± 0.508
5.522ThrVal: 5.522 ± 0.56
1.303ThrTrp: 1.303 ± 0.277
1.799ThrTyr: 1.799 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
7.073ValAla: 7.073 ± 0.857
0.434ValCys: 0.434 ± 0.166
5.398ValAsp: 5.398 ± 0.495
5.274ValGlu: 5.274 ± 0.626
2.482ValPhe: 2.482 ± 0.36
4.902ValGly: 4.902 ± 0.599
1.551ValHis: 1.551 ± 0.251
4.219ValIle: 4.219 ± 0.532
3.226ValLys: 3.226 ± 0.436
5.646ValLeu: 5.646 ± 0.61
1.365ValMet: 1.365 ± 0.329
2.606ValAsn: 2.606 ± 0.427
3.475ValPro: 3.475 ± 0.47
2.172ValGln: 2.172 ± 0.35
4.84ValArg: 4.84 ± 0.675
4.281ValSer: 4.281 ± 0.515
5.708ValThr: 5.708 ± 0.595
5.274ValVal: 5.274 ± 0.644
1.303ValTrp: 1.303 ± 0.26
2.296ValTyr: 2.296 ± 0.418
0.0ValXaa: 0.0 ± 0.0
Trp
1.799TrpAla: 1.799 ± 0.317
0.372TrpCys: 0.372 ± 0.133
1.799TrpAsp: 1.799 ± 0.303
0.931TrpGlu: 0.931 ± 0.247
0.869TrpPhe: 0.869 ± 0.238
1.799TrpGly: 1.799 ± 0.287
0.372TrpHis: 0.372 ± 0.145
1.055TrpIle: 1.055 ± 0.219
0.434TrpLys: 0.434 ± 0.163
1.613TrpLeu: 1.613 ± 0.326
0.372TrpMet: 0.372 ± 0.176
0.683TrpAsn: 0.683 ± 0.198
0.807TrpPro: 0.807 ± 0.257
1.117TrpGln: 1.117 ± 0.233
1.241TrpArg: 1.241 ± 0.293
0.745TrpSer: 0.745 ± 0.207
1.985TrpThr: 1.985 ± 0.393
2.234TrpVal: 2.234 ± 0.349
0.807TrpTrp: 0.807 ± 0.261
0.31TrpTyr: 0.31 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.544TyrAla: 2.544 ± 0.496
0.124TyrCys: 0.124 ± 0.087
1.303TyrAsp: 1.303 ± 0.25
2.482TyrGlu: 2.482 ± 0.396
0.558TyrPhe: 0.558 ± 0.141
2.668TyrGly: 2.668 ± 0.422
0.62TyrHis: 0.62 ± 0.211
1.737TyrIle: 1.737 ± 0.344
1.551TyrLys: 1.551 ± 0.333
2.234TyrLeu: 2.234 ± 0.368
0.683TyrMet: 0.683 ± 0.174
1.303TyrAsn: 1.303 ± 0.311
1.303TyrPro: 1.303 ± 0.287
1.055TyrGln: 1.055 ± 0.248
2.916TyrArg: 2.916 ± 0.432
1.551TyrSer: 1.551 ± 0.282
2.358TyrThr: 2.358 ± 0.407
1.799TyrVal: 1.799 ± 0.339
0.434TyrTrp: 0.434 ± 0.138
0.807TyrTyr: 0.807 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (16118 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski