Aquabacter spiritensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Aquabacter

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4631 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V2UXH8|A0A4V2UXH8_9RHIZ Uncharacterized protein OS=Aquabacter spiritensis OX=933073 GN=EDC64_109168 PE=4 SV=1
YYY2 pKa = 10.12DDD4 pKa = 3.72ATGLSVTQRR13 pKa = 11.84ALTVTAVGQSRR24 pKa = 11.84AYYY27 pKa = 10.63DDD29 pKa = 4.56DD30 pKa = 4.08PPLTYYY36 pKa = 10.61VGGAGLVNGDDD47 pKa = 4.03LAGALATAAGAASNVGGYYY66 pKa = 10.67ITQGTLAASSNYYY79 pKa = 10.13ITAFAPGTLTVTARR93 pKa = 11.84PLTVIANDDD102 pKa = 3.34SRR104 pKa = 11.84LFGDDD109 pKa = 4.14NPPLTYYY116 pKa = 10.65VGGDDD121 pKa = 3.52LVNGDDD127 pKa = 4.27LSGALVTTADDD138 pKa = 3.95ASLPGAYYY146 pKa = 9.19ILQGSLAVSLNYYY159 pKa = 10.85LTFDDD164 pKa = 4.26GEEE167 pKa = 4.16TVTARR172 pKa = 11.84MTSPALLAALLQPTFVQTGAADDD195 pKa = 3.57TGGTVVTFMPVGGAADDD212 pKa = 3.66SGGSDDD218 pKa = 3.27TGGGNDDD225 pKa = 3.71ANDDD229 pKa = 3.75DD230 pKa = 4.68IVYYY234 pKa = 10.09PRR236 pKa = 11.84LKKK239 pKa = 10.89KKK241 pKa = 10.96N

Molecular weight: 23.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3M5D9|A0A4R3M5D9_9RHIZ Diguanylate cyclase (GGDEF)-like protein OS=Aquabacter spiritensis OX=933073 GN=EDC64_101616 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATMNGRR28 pKa = 11.84KK29 pKa = 9.26IIASRR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.21RR41 pKa = 11.84LSAA44 pKa = 3.93

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4631

0

4631

1533501

29

4291

331.1

35.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.21 ± 0.057

0.83 ± 0.013

5.468 ± 0.032

5.069 ± 0.043

3.668 ± 0.025

9.335 ± 0.1

1.898 ± 0.02

4.718 ± 0.023

2.629 ± 0.031

10.412 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.019

2.191 ± 0.022

5.713 ± 0.049

2.739 ± 0.018

7.275 ± 0.057

5.05 ± 0.05

5.41 ± 0.058

7.778 ± 0.027

1.219 ± 0.015

2.114 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski