Panicum mosaic virus (strain United States/Kansas 109S) (PMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Panicovirus; Panicum mosaic virus

Average proteome isoelectric point is 8.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P90335|MP8_PMVK Probable movement protein p8 OS=Panicum mosaic virus (strain United States/Kansas 109S) OX=652599 GN=ORF2 PE=3 SV=1
MM1 pKa = 7.65EE2 pKa = 5.79LPLPEE7 pKa = 5.29DD8 pKa = 3.75VASVLYY14 pKa = 7.38LTPLAAVRR22 pKa = 11.84EE23 pKa = 4.29VNLWNGAPPPYY34 pKa = 10.99SMLQLWARR42 pKa = 11.84VDD44 pKa = 3.66HH45 pKa = 6.91AWAKK49 pKa = 10.71DD50 pKa = 3.59RR51 pKa = 11.84VGRR54 pKa = 11.84SYY56 pKa = 9.22PTRR59 pKa = 11.84KK60 pKa = 9.96SYY62 pKa = 10.69FKK64 pKa = 10.01STRR67 pKa = 11.84LLPRR71 pKa = 11.84TLYY74 pKa = 10.33RR75 pKa = 11.84QYY77 pKa = 11.23QSYY80 pKa = 10.66RR81 pKa = 11.84DD82 pKa = 4.06CQCLQEE88 pKa = 4.41TSPFTRR94 pKa = 11.84EE95 pKa = 3.65VHH97 pKa = 5.34HH98 pKa = 6.8TCVQLDD104 pKa = 4.02PLSPSTDD111 pKa = 3.62GEE113 pKa = 4.34HH114 pKa = 6.42SPSSGSPAVLRR125 pKa = 11.84QHH127 pKa = 6.81RR128 pKa = 11.84EE129 pKa = 2.99IWFF132 pKa = 3.78

Molecular weight: 15.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P89037|P15_PMVK Protein p15 OS=Panicum mosaic virus (strain United States/Kansas 109S) OX=652599 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.17NRR3 pKa = 11.84NGATPTRR10 pKa = 11.84GRR12 pKa = 11.84GKK14 pKa = 10.14RR15 pKa = 11.84AIPNPPRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84ARR26 pKa = 11.84GKK28 pKa = 9.3SVEE31 pKa = 4.3RR32 pKa = 11.84GSTPLQYY39 pKa = 10.01VTTLGPSRR47 pKa = 11.84PRR49 pKa = 11.84MGQGQGWQKK58 pKa = 10.71LSHH61 pKa = 6.39EE62 pKa = 4.46EE63 pKa = 4.41IILQVNSSTAADD75 pKa = 4.6TIQTIPIIPRR85 pKa = 11.84LSVPAGDD92 pKa = 3.63KK93 pKa = 10.52PIYY96 pKa = 10.11SGSAPHH102 pKa = 6.53LRR104 pKa = 11.84TIGSAFAIHH113 pKa = 6.89RR114 pKa = 11.84WRR116 pKa = 11.84ALSFEE121 pKa = 5.09WIPSCPTTTPGNLVLRR137 pKa = 11.84FYY139 pKa = 10.66PNYY142 pKa = 9.62STEE145 pKa = 4.23TPKK148 pKa = 10.49TLTDD152 pKa = 3.66LMDD155 pKa = 4.14SEE157 pKa = 5.24SLVLVPSLSGKK168 pKa = 7.14TYY170 pKa = 9.88RR171 pKa = 11.84PKK173 pKa = 10.34IEE175 pKa = 4.12TRR177 pKa = 11.84GNPPEE182 pKa = 4.72LRR184 pKa = 11.84NIDD187 pKa = 3.26ATAFSALSDD196 pKa = 3.9EE197 pKa = 5.08DD198 pKa = 3.67KK199 pKa = 11.36GDD201 pKa = 3.67YY202 pKa = 10.51SVGRR206 pKa = 11.84LVVGSSKK213 pKa = 10.38QAVVIQLGLLRR224 pKa = 11.84MRR226 pKa = 11.84YY227 pKa = 7.7SAEE230 pKa = 3.67MRR232 pKa = 11.84GATSISGVSAA242 pKa = 3.98

Molecular weight: 26.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1498

59

992

299.6

33.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.076 ± 0.871

1.869 ± 0.472

4.873 ± 0.607

5.207 ± 0.325

3.071 ± 0.529

5.741 ± 0.739

2.67 ± 0.484

4.673 ± 0.459

6.075 ± 1.151

9.546 ± 0.57

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.003 ± 0.13

3.271 ± 0.335

6.275 ± 1.08

4.206 ± 0.503

6.475 ± 1.159

7.744 ± 1.055

6.275 ± 0.842

6.876 ± 0.577

1.869 ± 0.332

4.139 ± 0.576
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski