Faba bean necrotic yellows virus (isolate Egyptian EV1-93) (FBNYV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Mulpavirales; Nanoviridae; Nanovirus; Faba bean necrotic yellows virus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9WIJ4|CLINK_FBNY1 Cell cycle link protein OS=Faba bean necrotic yellows virus (isolate Egyptian EV1-93) OX=291603 GN=DNA-C PE=1 SV=1
MM1 pKa = 7.61EE2 pKa = 4.03VRR4 pKa = 11.84FLLFLLLLVLVINPSLVVNMVFGYY28 pKa = 9.62MFGLLLRR35 pKa = 11.84NNYY38 pKa = 9.41SRR40 pKa = 11.84LKK42 pKa = 11.19AMIRR46 pKa = 11.84SNQTEE51 pKa = 4.23EE52 pKa = 3.82EE53 pKa = 4.41EE54 pKa = 4.28EE55 pKa = 3.83RR56 pKa = 11.84HH57 pKa = 5.54EE58 pKa = 4.9AVDD61 pKa = 3.41VSNPFEE67 pKa = 4.84DD68 pKa = 3.19VDD70 pKa = 3.83IDD72 pKa = 4.22VMQHH76 pKa = 6.11LKK78 pKa = 9.38TLGLDD83 pKa = 3.6SKK85 pKa = 11.45VDD87 pKa = 3.75EE88 pKa = 4.75EE89 pKa = 4.19DD90 pKa = 3.13VEE92 pKa = 4.3YY93 pKa = 10.66FRR95 pKa = 11.84RR96 pKa = 11.84FWQSMLRR103 pKa = 11.84NKK105 pKa = 10.2

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9WIJ9|U2_FBNY1 Protein U2 OS=Faba bean necrotic yellows virus (isolate Egyptian EV1-93) OX=291603 GN=DNA-U2 PE=3 SV=1
MM1 pKa = 7.37ASKK4 pKa = 10.16WNWSGTKK11 pKa = 9.85GRR13 pKa = 11.84RR14 pKa = 11.84TPRR17 pKa = 11.84RR18 pKa = 11.84PYY20 pKa = 9.37GRR22 pKa = 11.84PYY24 pKa = 10.15KK25 pKa = 10.65SSVPTTRR32 pKa = 11.84VVVHH36 pKa = 6.05QSAVLKK42 pKa = 10.21KK43 pKa = 10.18DD44 pKa = 3.39EE45 pKa = 4.34VVGTEE50 pKa = 4.23IKK52 pKa = 10.71PEE54 pKa = 4.08GDD56 pKa = 3.06VARR59 pKa = 11.84YY60 pKa = 9.51KK61 pKa = 9.79MKK63 pKa = 10.69KK64 pKa = 10.34VMLTCTLRR72 pKa = 11.84MAPGEE77 pKa = 4.04LVNYY81 pKa = 9.56LIVKK85 pKa = 9.1CNSPISSWSAAFTSPALLVKK105 pKa = 9.94EE106 pKa = 4.7SCQDD110 pKa = 3.44MITIIAKK117 pKa = 10.35GKK119 pKa = 9.48VEE121 pKa = 4.61SNGVAGTDD129 pKa = 3.49CTKK132 pKa = 10.72SFNRR136 pKa = 11.84FIKK139 pKa = 10.4LGAGITQTQHH149 pKa = 6.85LYY151 pKa = 9.5VVLYY155 pKa = 9.51TSVALKK161 pKa = 10.41AVLEE165 pKa = 4.06HH166 pKa = 6.11RR167 pKa = 11.84VYY169 pKa = 11.44VEE171 pKa = 3.73VV172 pKa = 3.87

Molecular weight: 19.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

2124

105

286

193.1

22.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.567 ± 0.518

2.589 ± 0.388

5.697 ± 0.585

7.345 ± 0.633

3.578 ± 0.32

5.556 ± 0.483

2.166 ± 0.263

6.309 ± 0.513

7.627 ± 0.448

8.522 ± 0.658

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.202 ± 0.193

4.379 ± 0.386

3.861 ± 0.328

3.578 ± 0.157

6.215 ± 0.625

6.356 ± 0.369

4.473 ± 0.599

6.921 ± 0.694

1.883 ± 0.253

5.179 ± 0.528
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski