Synergistes jonesii

Taxonomy: cellular organisms; Bacteria; Synergistetes; Synergistia; Synergistales; Synergistaceae; Synergistes

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2481 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A073IQ08|A0A073IQ08_9BACT Uracil phosphoribosyltransferase OS=Synergistes jonesii OX=2754 GN=upp PE=3 SV=1
MM1 pKa = 7.59SARR4 pKa = 11.84EE5 pKa = 3.83KK6 pKa = 10.37IAYY9 pKa = 9.5LKK11 pKa = 11.06GLIDD15 pKa = 4.2GQNLAAGSTDD25 pKa = 2.98KK26 pKa = 11.54AKK28 pKa = 10.65FYY30 pKa = 11.0AALVDD35 pKa = 3.97ALEE38 pKa = 4.33SLAVDD43 pKa = 3.58IEE45 pKa = 4.19KK46 pKa = 10.61HH47 pKa = 5.26EE48 pKa = 4.28EE49 pKa = 3.76VHH51 pKa = 7.08EE52 pKa = 4.06EE53 pKa = 3.88LNDD56 pKa = 3.64YY57 pKa = 10.97LDD59 pKa = 4.05QLDD62 pKa = 4.01EE63 pKa = 4.67DD64 pKa = 4.39VSKK67 pKa = 11.64LEE69 pKa = 4.96DD70 pKa = 4.02DD71 pKa = 4.72LGDD74 pKa = 3.95FLGEE78 pKa = 4.72GYY80 pKa = 10.73DD81 pKa = 5.19DD82 pKa = 5.67DD83 pKa = 5.31DD84 pKa = 3.84EE85 pKa = 5.29CRR87 pKa = 11.84EE88 pKa = 4.12HH89 pKa = 6.61HH90 pKa = 6.87HH91 pKa = 6.79HH92 pKa = 7.9DD93 pKa = 3.63EE94 pKa = 4.47DD95 pKa = 5.16EE96 pKa = 4.24EE97 pKa = 5.91DD98 pKa = 3.57GDD100 pKa = 3.98YY101 pKa = 11.77EE102 pKa = 4.25EE103 pKa = 5.64FDD105 pKa = 3.56EE106 pKa = 5.26EE107 pKa = 4.57EE108 pKa = 4.67YY109 pKa = 11.22EE110 pKa = 5.67SVTCPNCHH118 pKa = 6.2NDD120 pKa = 3.85FYY122 pKa = 11.44YY123 pKa = 10.6EE124 pKa = 3.57PAMYY128 pKa = 10.01KK129 pKa = 10.2EE130 pKa = 4.45EE131 pKa = 4.24EE132 pKa = 4.35EE133 pKa = 4.7LVCPHH138 pKa = 6.97CGKK141 pKa = 9.94QFKK144 pKa = 10.56LPEE147 pKa = 4.35DD148 pKa = 3.44

Molecular weight: 17.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A073J4M0|A0A073J4M0_9BACT ABC transporter permease OS=Synergistes jonesii OX=2754 GN=EH55_02580 PE=4 SV=1
MM1 pKa = 7.49GATAYY6 pKa = 9.15KK7 pKa = 9.87RR8 pKa = 11.84PAWAVTNQQILLMPEE23 pKa = 3.83IVRR26 pKa = 11.84RR27 pKa = 11.84QSWSVRR33 pKa = 11.84CIQASAVRR41 pKa = 11.84LIRR44 pKa = 11.84PRR46 pKa = 11.84GAITGKK52 pKa = 9.35QLQFIFDD59 pKa = 3.7SRR61 pKa = 11.84VICHH65 pKa = 6.77RR66 pKa = 11.84YY67 pKa = 7.49KK68 pKa = 10.98ARR70 pKa = 11.84VAAEE74 pKa = 3.83RR75 pKa = 11.84RR76 pKa = 11.84GKK78 pKa = 10.53ANNNVPHH85 pKa = 7.11RR86 pKa = 11.84NNVAQRR92 pKa = 11.84HH93 pKa = 4.66GSLYY97 pKa = 10.15IGAFLLISMTCGCGVHH113 pKa = 6.52LRR115 pKa = 11.84DD116 pKa = 3.14HH117 pKa = 5.97SVRR120 pKa = 11.84VKK122 pKa = 10.88EE123 pKa = 4.14PLCHH127 pKa = 6.53IAEE130 pKa = 4.73IDD132 pKa = 3.58PKK134 pKa = 10.7RR135 pKa = 11.84PGISRR140 pKa = 11.84EE141 pKa = 4.19QIGLPHH147 pKa = 6.77IKK149 pKa = 9.92KK150 pKa = 10.07EE151 pKa = 3.93LMAFCFDD158 pKa = 3.37IQAQAVLRR166 pKa = 11.84QCVGVVPAYY175 pKa = 10.78SPAKK179 pKa = 10.23LL180 pKa = 3.43

Molecular weight: 20.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2481

0

2481

784574

41

2230

316.2

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.471 ± 0.064

1.577 ± 0.021

5.141 ± 0.039

7.125 ± 0.055

4.099 ± 0.04

8.389 ± 0.05

1.573 ± 0.021

6.276 ± 0.044

5.751 ± 0.039

9.335 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.893 ± 0.027

3.127 ± 0.025

4.191 ± 0.03

2.303 ± 0.024

5.977 ± 0.053

5.93 ± 0.036

4.602 ± 0.026

7.008 ± 0.041

1.139 ± 0.02

3.093 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski