Vibrio phage VPp1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076GC75|A0A076GC75_9CAUD 5'-3' exonuclease OS=Vibrio phage VPp1 OX=1524880 GN=Q21_gp5 PE=4 SV=1
MM1 pKa = 7.86AIYY4 pKa = 10.02KK5 pKa = 10.1DD6 pKa = 3.11KK7 pKa = 10.99RR8 pKa = 11.84EE9 pKa = 4.09RR10 pKa = 11.84IGNIIHH16 pKa = 5.96TARR19 pKa = 11.84VQVLPFDD26 pKa = 3.94VWCPICEE33 pKa = 4.02EE34 pKa = 4.09HH35 pKa = 7.13HH36 pKa = 7.11DD37 pKa = 4.04GVMSSIDD44 pKa = 3.41DD45 pKa = 3.49TTIISEE51 pKa = 4.51CTCDD55 pKa = 3.87IDD57 pKa = 5.24DD58 pKa = 5.07FYY60 pKa = 11.44TEE62 pKa = 4.25EE63 pKa = 4.54EE64 pKa = 4.12YY65 pKa = 11.43DD66 pKa = 3.84DD67 pKa = 4.07VVEE70 pKa = 5.34EE71 pKa = 4.01RR72 pKa = 11.84DD73 pKa = 3.11RR74 pKa = 11.84WEE76 pKa = 3.77IKK78 pKa = 10.45AEE80 pKa = 3.96EE81 pKa = 4.22FEE83 pKa = 4.31EE84 pKa = 4.29EE85 pKa = 3.89RR86 pKa = 11.84DD87 pKa = 3.4EE88 pKa = 5.22AVADD92 pKa = 3.72KK93 pKa = 11.2DD94 pKa = 4.37RR95 pKa = 11.84IEE97 pKa = 3.9QEE99 pKa = 4.25YY100 pKa = 9.14VQEE103 pKa = 4.13IQTLRR108 pKa = 11.84DD109 pKa = 3.76NNEE112 pKa = 3.82LTHH115 pKa = 6.01NTLEE119 pKa = 4.27IEE121 pKa = 4.24RR122 pKa = 11.84EE123 pKa = 3.94EE124 pKa = 3.95HH125 pKa = 6.0QEE127 pKa = 4.09AIEE130 pKa = 4.21LRR132 pKa = 11.84DD133 pKa = 3.85SIIDD137 pKa = 3.52EE138 pKa = 4.56LEE140 pKa = 3.97AKK142 pKa = 10.13LRR144 pKa = 11.84EE145 pKa = 4.27LEE147 pKa = 4.21GG148 pKa = 3.96

Molecular weight: 17.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076G5J3|A0A076G5J3_9CAUD Uncharacterized protein OS=Vibrio phage VPp1 OX=1524880 GN=Q21_gp44 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 9.36ATQKK6 pKa = 10.58VMRR9 pKa = 11.84QSQFPQGGWRR19 pKa = 11.84KK20 pKa = 9.56RR21 pKa = 11.84DD22 pKa = 3.22ATLYY26 pKa = 10.88SSFKK30 pKa = 9.89GSKK33 pKa = 9.25KK34 pKa = 10.3ARR36 pKa = 11.84SKK38 pKa = 11.19LLTHH42 pKa = 6.97RR43 pKa = 11.84QGDD46 pKa = 4.1CKK48 pKa = 11.27ANATKK53 pKa = 10.51ALSEE57 pKa = 4.15ALRR60 pKa = 11.84ALL62 pKa = 3.94

Molecular weight: 6.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

15408

41

927

230.0

25.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.905 ± 0.45

1.097 ± 0.148

6.497 ± 0.201

7.431 ± 0.403

3.771 ± 0.209

7.308 ± 0.313

2.096 ± 0.176

5.322 ± 0.168

7.237 ± 0.361

7.704 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.81 ± 0.167

5.387 ± 0.244

3.621 ± 0.188

4.089 ± 0.247

4.563 ± 0.177

5.283 ± 0.348

5.938 ± 0.304

6.575 ± 0.194

1.532 ± 0.146

3.836 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski