Trichuris trichiura (Whipworm) (Trichocephalus trichiurus)
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9625 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A077ZH41|A0A077ZH41_TRITR CSD domain containing protein OS=Trichuris trichiura OX=36087 GN=TTRE_0000731701 PE=4 SV=1
MM1 pKa = 8.07 ADD3 pKa = 3.57 TDD5 pKa = 4.22 LSTNQLLDD13 pKa = 3.46 VPLEE17 pKa = 4.02 DD18 pKa = 3.55 QYY20 pKa = 11.99 GGEE23 pKa = 4.03 VAMEE27 pKa = 4.18 NGCEE31 pKa = 3.73 ITALSMLLNYY41 pKa = 10.0 YY42 pKa = 10.71 GYY44 pKa = 8.86 GTDD47 pKa = 3.56 KK48 pKa = 11.61 NEE50 pKa = 4.85 LVDD53 pKa = 5.39 LLDD56 pKa = 4.22 FVPLYY61 pKa = 10.79 EE62 pKa = 4.55 DD63 pKa = 3.42 AEE65 pKa = 4.34 KK66 pKa = 10.49 RR67 pKa = 11.84 IHH69 pKa = 6.75 GNPHH73 pKa = 6.72 DD74 pKa = 4.2 GFVGNIYY81 pKa = 10.38 EE82 pKa = 4.67 GYY84 pKa = 9.18 EE85 pKa = 3.9 AMGVAVEE92 pKa = 5.21 PIAEE96 pKa = 4.24 VANEE100 pKa = 3.98 VVQDD104 pKa = 3.87 NQKK107 pKa = 10.01 VVANNDD113 pKa = 2.95 TSFDD117 pKa = 3.7 DD118 pKa = 4.01 LVRR121 pKa = 11.84 VIQTGTPVWVITTVDD136 pKa = 4.02 FQVPTEE142 pKa = 4.12 DD143 pKa = 6.16 DD144 pKa = 3.37 FRR146 pKa = 11.84 TWQTTSGEE154 pKa = 4.24 IEE156 pKa = 4.31 VSPLCHH162 pKa = 6.15 AVIITGVDD170 pKa = 3.37 EE171 pKa = 4.48 EE172 pKa = 4.4 NVYY175 pKa = 11.18 VNDD178 pKa = 3.87 PYY180 pKa = 11.19 GYY182 pKa = 10.2 KK183 pKa = 10.36 NRR185 pKa = 11.84 VVDD188 pKa = 3.54 RR189 pKa = 11.84 TDD191 pKa = 3.38 FEE193 pKa = 4.95 EE194 pKa = 5.3 IYY196 pKa = 10.88 QLMGSEE202 pKa = 4.26 SLYY205 pKa = 11.31 LEE207 pKa = 5.13 DD208 pKa = 5.14 KK209 pKa = 11.1
Molecular weight: 23.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.77
IPC_protein 3.77
Toseland 3.554
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.656
Rodwell 3.592
Grimsley 3.465
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.884
Patrickios 0.668
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.781
Protein with the highest isoelectric point:
>tr|A0A077ZKX5|A0A077ZKX5_TRITR CbiA and ParBc and KorB domain containing protein OS=Trichuris trichiura OX=36087 GN=TTRE_0000757601 PE=4 SV=1
MM1 pKa = 6.94 QWRR4 pKa = 11.84 SVAWRR9 pKa = 11.84 GVAWRR14 pKa = 11.84 GVAWRR19 pKa = 11.84 GGAGSGVAWRR29 pKa = 11.84 GVAWGVRR36 pKa = 11.84 CGGVGRR42 pKa = 11.84 GVAWRR47 pKa = 11.84 GGVGRR52 pKa = 11.84 GVAWRR57 pKa = 11.84 GGVGRR62 pKa = 11.84 GVAWRR67 pKa = 11.84 GVAWRR72 pKa = 11.84 GG73 pKa = 3.16
Molecular weight: 7.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.444
IPC2_protein 10.965
IPC_protein 12.574
Toseland 12.735
ProMoST 13.247
Dawson 12.735
Bjellqvist 12.735
Wikipedia 13.217
Rodwell 12.237
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.735
DTASelect 12.735
Thurlkill 12.735
EMBOSS 13.247
Sillero 12.735
Patrickios 11.974
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9625
0
9625
4182167
31
7532
434.5
48.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.122 ± 0.026
2.487 ± 0.024
5.258 ± 0.02
6.406 ± 0.03
4.376 ± 0.017
5.766 ± 0.03
2.312 ± 0.012
5.197 ± 0.019
5.675 ± 0.025
9.761 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.448 ± 0.01
4.252 ± 0.014
4.699 ± 0.023
3.97 ± 0.018
5.887 ± 0.018
7.934 ± 0.029
5.23 ± 0.017
6.821 ± 0.018
1.233 ± 0.009
3.162 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here