Bat associated circovirus 1
Average proteome isoelectric point is 8.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4L9E4|R4L9E4_9CIRC Capsid protein OS=Bat associated circovirus 1 OX=1868218 GN=cap PE=3 SV=1
MM1 pKa = 7.46 EE2 pKa = 4.57 MAGHH6 pKa = 6.25 PCEE9 pKa = 4.97 RR10 pKa = 11.84 YY11 pKa = 10.13 CFTINNYY18 pKa = 9.0 SEE20 pKa = 4.06 EE21 pKa = 4.62 DD22 pKa = 3.4 IEE24 pKa = 4.23 AVKK27 pKa = 10.96 AFLVPDD33 pKa = 3.56 NAEE36 pKa = 3.78 YY37 pKa = 10.58 AIVGKK42 pKa = 10.44 EE43 pKa = 3.62 KK44 pKa = 11.17 GEE46 pKa = 4.05 NGTPHH51 pKa = 6.48 LQGFVNLKK59 pKa = 10.05 KK60 pKa = 10.61 KK61 pKa = 9.7 MRR63 pKa = 11.84 FNPFKK68 pKa = 10.66 AALGGRR74 pKa = 11.84 AHH76 pKa = 7.3 IEE78 pKa = 3.82 QARR81 pKa = 11.84 GTDD84 pKa = 3.67 LDD86 pKa = 3.68 NKK88 pKa = 9.98 RR89 pKa = 11.84 YY90 pKa = 9.18 CSKK93 pKa = 11.18 GGDD96 pKa = 3.5 LLLEE100 pKa = 4.22 VGEE103 pKa = 4.64 PGKK106 pKa = 10.33 QGKK109 pKa = 9.96 RR110 pKa = 11.84 SDD112 pKa = 3.51 LKK114 pKa = 11.13 EE115 pKa = 3.9 AVTLLNSGANMTAVARR131 pKa = 11.84 AYY133 pKa = 9.02 PEE135 pKa = 3.65 VFIRR139 pKa = 11.84 YY140 pKa = 8.65 GRR142 pKa = 11.84 GLRR145 pKa = 11.84 DD146 pKa = 3.45 YY147 pKa = 10.83 VITAGLSQQRR157 pKa = 11.84 AWKK160 pKa = 8.44 TEE162 pKa = 3.34 VHH164 pKa = 6.42 VIVGVPGVGKK174 pKa = 10.03 SRR176 pKa = 11.84 HH177 pKa = 4.83 VSEE180 pKa = 4.27 QHH182 pKa = 6.42 SDD184 pKa = 3.42 IYY186 pKa = 10.2 WKK188 pKa = 10.17 PRR190 pKa = 11.84 GKK192 pKa = 8.98 WWDD195 pKa = 4.15 GYY197 pKa = 10.73 CNQEE201 pKa = 4.39 VVCLDD206 pKa = 4.83 DD207 pKa = 3.91 YY208 pKa = 11.47 YY209 pKa = 11.91 GWIPYY214 pKa = 10.31 DD215 pKa = 4.05 DD216 pKa = 5.12 LLRR219 pKa = 11.84 LCDD222 pKa = 4.13 RR223 pKa = 11.84 YY224 pKa = 10.18 PLRR227 pKa = 11.84 VEE229 pKa = 4.36 TKK231 pKa = 8.38 GGTVSFVAKK240 pKa = 10.32 KK241 pKa = 10.3 IYY243 pKa = 8.87 ITSNKK248 pKa = 8.9 QIKK251 pKa = 9.38 DD252 pKa = 2.98 WYY254 pKa = 10.2 NFEE257 pKa = 4.17 EE258 pKa = 4.59 LKK260 pKa = 10.53 VDD262 pKa = 3.39 PRR264 pKa = 11.84 ALYY267 pKa = 10.43 RR268 pKa = 11.84 RR269 pKa = 11.84 VTSYY273 pKa = 11.31 KK274 pKa = 9.81 VMRR277 pKa = 11.84 EE278 pKa = 3.69 GGEE281 pKa = 4.09 LYY283 pKa = 10.7 DD284 pKa = 4.79 VVMTGDD290 pKa = 3.31 NKK292 pKa = 10.54 INYY295 pKa = 8.4
Molecular weight: 33.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.586
IPC2_protein 7.878
IPC_protein 7.775
Toseland 7.585
ProMoST 8.39
Dawson 8.492
Bjellqvist 8.697
Wikipedia 8.478
Rodwell 8.521
Grimsley 7.556
Solomon 8.609
Lehninger 8.595
Nozaki 8.785
DTASelect 8.492
Thurlkill 8.551
EMBOSS 8.682
Sillero 8.829
Patrickios 4.202
IPC_peptide 8.595
IPC2_peptide 7.541
IPC2.peptide.svr19 7.805
Protein with the highest isoelectric point:
>tr|R4L9E4|R4L9E4_9CIRC Capsid protein OS=Bat associated circovirus 1 OX=1868218 GN=cap PE=3 SV=1
MM1 pKa = 7.94 PIRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 SRR8 pKa = 11.84 YY9 pKa = 7.19 SRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 WRR16 pKa = 11.84 RR17 pKa = 11.84 NTRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 VARR26 pKa = 11.84 GAYY29 pKa = 8.5 RR30 pKa = 11.84 WRR32 pKa = 11.84 RR33 pKa = 11.84 KK34 pKa = 10.04 NGIINVRR41 pKa = 11.84 LSATKK46 pKa = 10.02 DD47 pKa = 2.63 WTMASTTAEE56 pKa = 4.56 GYY58 pKa = 9.63 NVARR62 pKa = 11.84 LEE64 pKa = 4.28 VNLRR68 pKa = 11.84 QFMPAGPGSAINTKK82 pKa = 10.08 SIPWAYY88 pKa = 10.59 YY89 pKa = 9.97 RR90 pKa = 11.84 IRR92 pKa = 11.84 KK93 pKa = 7.77 MKK95 pKa = 10.71 FEE97 pKa = 4.98 ILPKK101 pKa = 9.69 MIPAQTPYY109 pKa = 10.51 RR110 pKa = 11.84 YY111 pKa = 10.16 GSTAIYY117 pKa = 10.62 LGMQAPAPTQGKK129 pKa = 7.78 TYY131 pKa = 10.3 DD132 pKa = 3.39 PHH134 pKa = 7.41 LKK136 pKa = 9.95 HH137 pKa = 6.48 VKK139 pKa = 10.06 QNMSGLITDD148 pKa = 3.24 QLKK151 pKa = 10.5 RR152 pKa = 11.84 YY153 pKa = 7.39 FTPKK157 pKa = 9.73 PDD159 pKa = 3.91 LDD161 pKa = 5.18 SITSTAWFQPNNKK174 pKa = 9.35 ANQVWINMTNDD185 pKa = 3.67 NITHH189 pKa = 5.88 GQVGWSMEE197 pKa = 4.49 RR198 pKa = 11.84 ISNMAQNFKK207 pKa = 10.53 IRR209 pKa = 11.84 VTLYY213 pKa = 8.8 VQFRR217 pKa = 11.84 EE218 pKa = 4.41 FNLIDD223 pKa = 3.88 YY224 pKa = 7.74 PAQAPLLVDD233 pKa = 4.1 EE234 pKa = 5.54 EE235 pKa = 4.38 PSEE238 pKa = 4.17
Molecular weight: 27.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.309
IPC2_protein 10.101
IPC_protein 11.038
Toseland 10.979
ProMoST 10.935
Dawson 11.067
Bjellqvist 10.877
Wikipedia 11.374
Rodwell 11.155
Grimsley 11.125
Solomon 11.286
Lehninger 11.228
Nozaki 10.965
DTASelect 10.877
Thurlkill 10.994
EMBOSS 11.418
Sillero 11.008
Patrickios 10.847
IPC_peptide 11.286
IPC2_peptide 9.97
IPC2.peptide.svr19 8.254
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
533
238
295
266.5
30.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.754 ± 0.473
1.126 ± 0.659
4.878 ± 0.888
5.441 ± 1.217
2.814 ± 0.074
7.317 ± 1.578
1.689 ± 0.251
5.441 ± 0.75
6.942 ± 0.866
6.379 ± 0.537
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.002 ± 0.702
5.629 ± 0.641
5.066 ± 0.97
3.752 ± 0.755
9.006 ± 1.615
4.503 ± 0.562
5.441 ± 0.996
6.567 ± 1.384
2.439 ± 0.294
5.816 ± 0.453
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here