Solanum nodiflorum mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1P7XK87|A0A1P7XK87_9VIRU Putative RNA silencing suppressor OS=Solanum nodiflorum mottle virus OX=12471 GN=P1 PE=4 SV=1
MM1 pKa = 7.76PSIILEE7 pKa = 4.51IISSYY12 pKa = 8.42TVNGAPCDD20 pKa = 3.5ISEE23 pKa = 4.61PLPFSRR29 pKa = 11.84EE30 pKa = 4.01LIDD33 pKa = 3.6TKK35 pKa = 11.17KK36 pKa = 10.94LFIDD40 pKa = 4.46LPGDD44 pKa = 3.71NLPDD48 pKa = 3.44SYY50 pKa = 10.92RR51 pKa = 11.84VYY53 pKa = 11.34GRR55 pKa = 11.84FNPACISAVEE65 pKa = 3.82LHH67 pKa = 6.57IVCANCGASYY77 pKa = 10.31WKK79 pKa = 10.2FAEE82 pKa = 4.27LKK84 pKa = 10.25NVEE87 pKa = 4.5LVEE90 pKa = 4.02FDD92 pKa = 3.65YY93 pKa = 11.64VEE95 pKa = 4.92SITRR99 pKa = 11.84PGSLPSVYY107 pKa = 10.07TYY109 pKa = 11.25TNLVDD114 pKa = 4.57AEE116 pKa = 4.46LTVEE120 pKa = 4.13VCDD123 pKa = 4.08EE124 pKa = 4.41SEE126 pKa = 4.28TCSEE130 pKa = 3.99HH131 pKa = 6.86HH132 pKa = 7.12LNTILGLADD141 pKa = 3.34AAGEE145 pKa = 4.02FSLDD149 pKa = 3.48SDD151 pKa = 4.84SDD153 pKa = 4.0EE154 pKa = 4.36EE155 pKa = 4.42DD156 pKa = 3.4QLSLLYY162 pKa = 10.65RR163 pKa = 11.84GDD165 pKa = 3.83LSLEE169 pKa = 3.73RR170 pKa = 11.84DD171 pKa = 3.39AATDD175 pKa = 3.0WRR177 pKa = 11.84HH178 pKa = 6.48RR179 pKa = 11.84EE180 pKa = 3.64TDD182 pKa = 3.37EE183 pKa = 3.99NN184 pKa = 4.01

Molecular weight:
20.56 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1P7XK78|A0A1P7XK78_9VIRU N-terminal protein OS=Solanum nodiflorum mottle virus OX=12471 PE=4 SV=1
MM1 pKa = 7.1VKK3 pKa = 10.0RR4 pKa = 11.84RR5 pKa = 11.84PRR7 pKa = 11.84LRR9 pKa = 11.84QLTVMEE15 pKa = 4.7RR16 pKa = 11.84VTEE19 pKa = 4.14RR20 pKa = 11.84NPPARR25 pKa = 11.84KK26 pKa = 8.95SRR28 pKa = 11.84PRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 9.8GGQTTMVTAPIAGTMVYY51 pKa = 9.94RR52 pKa = 11.84RR53 pKa = 11.84NPILMNGRR61 pKa = 11.84RR62 pKa = 11.84GITVCHH68 pKa = 6.23SEE70 pKa = 4.2VVLAVQSTATAFSATSVTLAPNTFSWLSVMGALFSKK106 pKa = 9.43WRR108 pKa = 11.84WISLRR113 pKa = 11.84ATYY116 pKa = 10.53VPEE119 pKa = 4.29TATTTPGIVAMAFQYY134 pKa = 11.43DD135 pKa = 3.8NTDD138 pKa = 3.45TLPVGNAGMSSLYY151 pKa = 10.31GYY153 pKa = 10.95VSGAPWAGFEE163 pKa = 4.18GSKK166 pKa = 10.88LLTEE170 pKa = 4.86KK171 pKa = 9.26PTTPIPSGAIATQLDD186 pKa = 4.13CTNFGLKK193 pKa = 8.93WYY195 pKa = 9.51QYY197 pKa = 9.43KK198 pKa = 10.7AVMPAGDD205 pKa = 4.11SGNLYY210 pKa = 10.51VPAQLIVGNLGANNPLRR227 pKa = 11.84YY228 pKa = 10.01GEE230 pKa = 4.01VHH232 pKa = 5.56IQYY235 pKa = 9.13EE236 pKa = 4.48VEE238 pKa = 4.7FIEE241 pKa = 5.57PIPPTSNALRR251 pKa = 11.84SVTDD255 pKa = 4.06EE256 pKa = 3.44ILKK259 pKa = 9.98RR260 pKa = 11.84EE261 pKa = 3.83RR262 pKa = 11.84RR263 pKa = 11.84EE264 pKa = 3.9LCVNEE269 pKa = 4.14PPAVVVNTEE278 pKa = 3.63VLLPSKK284 pKa = 9.29EE285 pKa = 4.35TEE287 pKa = 3.43IDD289 pKa = 3.36KK290 pKa = 11.58AEE292 pKa = 4.96LIPP295 pKa = 5.04

Molecular weight:
32.54 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2039

184

981

509.8

56.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.376 ± 0.461

1.717 ± 0.27

4.561 ± 0.664

7.504 ± 0.516

3.041 ± 0.344

7.013 ± 0.521

1.864 ± 0.388

4.512 ± 0.197

5.542 ± 0.602

10.152 ± 0.412

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.109 ± 0.248

3.335 ± 0.359

6.13 ± 0.504

2.501 ± 0.235

5.64 ± 0.424

10.495 ± 1.191

5.444 ± 0.91

7.013 ± 0.462

1.913 ± 0.326

3.139 ± 0.166

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski