Solanum nodiflorum mottle virus
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P7XK87|A0A1P7XK87_9VIRU Putative RNA silencing suppressor OS=Solanum nodiflorum mottle virus OX=12471 GN=P1 PE=4 SV=1
MM1 pKa = 7.76 PSIILEE7 pKa = 4.51 IISSYY12 pKa = 8.42 TVNGAPCDD20 pKa = 3.5 ISEE23 pKa = 4.61 PLPFSRR29 pKa = 11.84 EE30 pKa = 4.01 LIDD33 pKa = 3.6 TKK35 pKa = 11.17 KK36 pKa = 10.94 LFIDD40 pKa = 4.46 LPGDD44 pKa = 3.71 NLPDD48 pKa = 3.44 SYY50 pKa = 10.92 RR51 pKa = 11.84 VYY53 pKa = 11.34 GRR55 pKa = 11.84 FNPACISAVEE65 pKa = 3.82 LHH67 pKa = 6.57 IVCANCGASYY77 pKa = 10.31 WKK79 pKa = 10.2 FAEE82 pKa = 4.27 LKK84 pKa = 10.25 NVEE87 pKa = 4.5 LVEE90 pKa = 4.02 FDD92 pKa = 3.65 YY93 pKa = 11.64 VEE95 pKa = 4.92 SITRR99 pKa = 11.84 PGSLPSVYY107 pKa = 10.07 TYY109 pKa = 11.25 TNLVDD114 pKa = 4.57 AEE116 pKa = 4.46 LTVEE120 pKa = 4.13 VCDD123 pKa = 4.08 EE124 pKa = 4.41 SEE126 pKa = 4.28 TCSEE130 pKa = 3.99 HH131 pKa = 6.86 HH132 pKa = 7.12 LNTILGLADD141 pKa = 3.34 AAGEE145 pKa = 4.02 FSLDD149 pKa = 3.48 SDD151 pKa = 4.84 SDD153 pKa = 4.0 EE154 pKa = 4.36 EE155 pKa = 4.42 DD156 pKa = 3.4 QLSLLYY162 pKa = 10.65 RR163 pKa = 11.84 GDD165 pKa = 3.83 LSLEE169 pKa = 3.73 RR170 pKa = 11.84 DD171 pKa = 3.39 AATDD175 pKa = 3.0 WRR177 pKa = 11.84 HH178 pKa = 6.48 RR179 pKa = 11.84 EE180 pKa = 3.64 TDD182 pKa = 3.37 EE183 pKa = 3.99 NN184 pKa = 4.01
Molecular weight: 20.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.829
IPC2_protein 4.088
IPC_protein 4.05
Toseland 3.859
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.91
Rodwell 3.884
Grimsley 3.77
Solomon 4.012
Lehninger 3.961
Nozaki 4.126
DTASelect 4.304
Thurlkill 3.884
EMBOSS 3.923
Sillero 4.164
Patrickios 1.214
IPC_peptide 4.012
IPC2_peptide 4.151
IPC2.peptide.svr19 4.052
Protein with the highest isoelectric point:
>tr|A0A1P7XK78|A0A1P7XK78_9VIRU N-terminal protein OS=Solanum nodiflorum mottle virus OX=12471 PE=4 SV=1
MM1 pKa = 7.1 VKK3 pKa = 10.0 RR4 pKa = 11.84 RR5 pKa = 11.84 PRR7 pKa = 11.84 LRR9 pKa = 11.84 QLTVMEE15 pKa = 4.7 RR16 pKa = 11.84 VTEE19 pKa = 4.14 RR20 pKa = 11.84 NPPARR25 pKa = 11.84 KK26 pKa = 8.95 SRR28 pKa = 11.84 PRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 KK34 pKa = 9.8 GGQTTMVTAPIAGTMVYY51 pKa = 9.94 RR52 pKa = 11.84 RR53 pKa = 11.84 NPILMNGRR61 pKa = 11.84 RR62 pKa = 11.84 GITVCHH68 pKa = 6.23 SEE70 pKa = 4.2 VVLAVQSTATAFSATSVTLAPNTFSWLSVMGALFSKK106 pKa = 9.43 WRR108 pKa = 11.84 WISLRR113 pKa = 11.84 ATYY116 pKa = 10.53 VPEE119 pKa = 4.29 TATTTPGIVAMAFQYY134 pKa = 11.43 DD135 pKa = 3.8 NTDD138 pKa = 3.45 TLPVGNAGMSSLYY151 pKa = 10.31 GYY153 pKa = 10.95 VSGAPWAGFEE163 pKa = 4.18 GSKK166 pKa = 10.88 LLTEE170 pKa = 4.86 KK171 pKa = 9.26 PTTPIPSGAIATQLDD186 pKa = 4.13 CTNFGLKK193 pKa = 8.93 WYY195 pKa = 9.51 QYY197 pKa = 9.43 KK198 pKa = 10.7 AVMPAGDD205 pKa = 4.11 SGNLYY210 pKa = 10.51 VPAQLIVGNLGANNPLRR227 pKa = 11.84 YY228 pKa = 10.01 GEE230 pKa = 4.01 VHH232 pKa = 5.56 IQYY235 pKa = 9.13 EE236 pKa = 4.48 VEE238 pKa = 4.7 FIEE241 pKa = 5.57 PIPPTSNALRR251 pKa = 11.84 SVTDD255 pKa = 4.06 EE256 pKa = 3.44 ILKK259 pKa = 9.98 RR260 pKa = 11.84 EE261 pKa = 3.83 RR262 pKa = 11.84 RR263 pKa = 11.84 EE264 pKa = 3.9 LCVNEE269 pKa = 4.14 PPAVVVNTEE278 pKa = 3.63 VLLPSKK284 pKa = 9.29 EE285 pKa = 4.35 TEE287 pKa = 3.43 IDD289 pKa = 3.36 KK290 pKa = 11.58 AEE292 pKa = 4.96 LIPP295 pKa = 5.04
Molecular weight: 32.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.915
IPC2_protein 9.092
IPC_protein 9.18
Toseland 9.575
ProMoST 9.443
Dawson 9.882
Bjellqvist 9.648
Wikipedia 10.101
Rodwell 10.058
Grimsley 9.984
Solomon 9.926
Lehninger 9.882
Nozaki 9.663
DTASelect 9.619
Thurlkill 9.706
EMBOSS 10.014
Sillero 9.823
Patrickios 5.067
IPC_peptide 9.911
IPC2_peptide 8.463
IPC2.peptide.svr19 7.983
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2039
184
981
509.8
56.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.376 ± 0.461
1.717 ± 0.27
4.561 ± 0.664
7.504 ± 0.516
3.041 ± 0.344
7.013 ± 0.521
1.864 ± 0.388
4.512 ± 0.197
5.542 ± 0.602
10.152 ± 0.412
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.109 ± 0.248
3.335 ± 0.359
6.13 ± 0.504
2.501 ± 0.235
5.64 ± 0.424
10.495 ± 1.191
5.444 ± 0.91
7.013 ± 0.462
1.913 ± 0.326
3.139 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here