Tetrapisispora phaffii (strain ATCC 24235 / CBS 4417 / NBRC 1672 / NRRL Y-8282 / UCD 70-5) (Yeast) (Fabospora phaffii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Tetrapisispora; Tetrapisispora phaffii

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5238 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8BUJ6|G8BUJ6_TETPH Nop domain-containing protein OS=Tetrapisispora phaffii (strain ATCC 24235 / CBS 4417 / NBRC 1672 / NRRL Y-8282 / UCD 70-5) OX=1071381 GN=TPHA0F03010 PE=3 SV=1
MM1 pKa = 7.02LTEE4 pKa = 4.6NEE6 pKa = 4.82HH7 pKa = 6.67ILNEE11 pKa = 4.03NPTIFSTISKK21 pKa = 8.18TVSDD25 pKa = 4.57TISYY29 pKa = 10.26LSSIDD34 pKa = 3.48NRR36 pKa = 11.84ASNVAVDD43 pKa = 4.15SAQKK47 pKa = 10.89SFDD50 pKa = 4.21AEE52 pKa = 4.07PLDD55 pKa = 3.61NLIAVFIIWFIVFFVLLFFIGLVVVVLLNYY85 pKa = 10.23RR86 pKa = 11.84NSSNNVYY93 pKa = 10.81NDD95 pKa = 3.72DD96 pKa = 3.63KK97 pKa = 11.81LEE99 pKa = 4.15YY100 pKa = 10.95NEE102 pKa = 4.54DD103 pKa = 3.33AYY105 pKa = 11.79YY106 pKa = 11.07DD107 pKa = 4.73DD108 pKa = 6.49DD109 pKa = 7.31DD110 pKa = 6.63DD111 pKa = 6.49DD112 pKa = 4.28EE113 pKa = 5.59YY114 pKa = 11.66YY115 pKa = 11.21YY116 pKa = 11.2SDD118 pKa = 3.91EE119 pKa = 4.41EE120 pKa = 4.52YY121 pKa = 10.88SSKK124 pKa = 10.77SHH126 pKa = 6.51SKK128 pKa = 11.1SNDD131 pKa = 3.59DD132 pKa = 4.03IEE134 pKa = 4.76KK135 pKa = 10.06QLSSAVV141 pKa = 3.43

Molecular weight: 16.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8BNC5|G8BNC5_TETPH Uncharacterized protein OS=Tetrapisispora phaffii (strain ATCC 24235 / CBS 4417 / NBRC 1672 / NRRL Y-8282 / UCD 70-5) OX=1071381 GN=TPHA0A03260 PE=4 SV=1
MM1 pKa = 7.4RR2 pKa = 11.84NKK4 pKa = 8.76WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.32RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.71RR15 pKa = 11.84KK16 pKa = 8.27RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.19VRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 10.98

Molecular weight: 3.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5238

0

5238

2672966

25

4901

510.3

57.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.935 ± 0.027

1.113 ± 0.012

6.087 ± 0.023

6.656 ± 0.035

4.4 ± 0.023

4.595 ± 0.029

1.944 ± 0.01

7.409 ± 0.031

7.776 ± 0.034

9.473 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.039 ± 0.012

7.34 ± 0.043

3.736 ± 0.02

3.667 ± 0.025

3.876 ± 0.02

8.982 ± 0.046

5.931 ± 0.053

5.461 ± 0.023

0.941 ± 0.009

3.641 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski