Faba bean necrotic yellows virus (isolate SV292-88) (FBNYV)
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P0CK61|REP9_FBNY2 Para-Rep C9 OS=Faba bean necrotic yellows virus (isolate SV292-88) OX=291604 GN=C9 PE=3 SV=1
MM1 pKa = 6.85 GLKK4 pKa = 10.18 YY5 pKa = 10.39 FSHH8 pKa = 7.43 LPEE11 pKa = 4.26 EE12 pKa = 4.28 LRR14 pKa = 11.84 QKK16 pKa = 10.02 IVHH19 pKa = 6.74 DD20 pKa = 3.96 HH21 pKa = 6.09 LQQEE25 pKa = 4.51 RR26 pKa = 11.84 KK27 pKa = 9.8 KK28 pKa = 10.67 EE29 pKa = 3.93 FLEE32 pKa = 4.07 KK33 pKa = 10.36 AIEE36 pKa = 4.35 DD37 pKa = 3.56 SCRR40 pKa = 11.84 RR41 pKa = 11.84 HH42 pKa = 5.71 VSLLKK47 pKa = 10.29 SDD49 pKa = 4.59 PSPSEE54 pKa = 4.52 LYY56 pKa = 10.79 ALSKK60 pKa = 10.73 FLDD63 pKa = 3.59 SLADD67 pKa = 3.67 YY68 pKa = 10.66 VGKK71 pKa = 10.24 QFNTRR76 pKa = 11.84 CLIKK80 pKa = 9.87 WKK82 pKa = 10.34 KK83 pKa = 10.13 DD84 pKa = 3.02 VPANIKK90 pKa = 10.34 FEE92 pKa = 4.23 VMEE95 pKa = 4.24 EE96 pKa = 3.77 QHH98 pKa = 6.08 LRR100 pKa = 11.84 LYY102 pKa = 11.17 GFVDD106 pKa = 4.03 MDD108 pKa = 4.22 DD109 pKa = 3.83 LLCRR113 pKa = 11.84 EE114 pKa = 4.26 VLPPEE119 pKa = 4.33 EE120 pKa = 5.79 DD121 pKa = 4.23 DD122 pKa = 5.7 DD123 pKa = 3.85 ITYY126 pKa = 10.57 EE127 pKa = 4.33 DD128 pKa = 4.43 GMIVNCSEE136 pKa = 3.96 LDD138 pKa = 3.52 KK139 pKa = 11.35 LFEE142 pKa = 4.25 ALGIKK147 pKa = 9.35 VVYY150 pKa = 9.0 ITVSKK155 pKa = 11.24 NCICTPLNKK164 pKa = 10.32 DD165 pKa = 3.1 IVISS169 pKa = 3.83
Molecular weight: 19.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.043
IPC2_protein 5.118
IPC_protein 5.041
Toseland 4.991
ProMoST 5.181
Dawson 5.041
Bjellqvist 5.169
Wikipedia 4.914
Rodwell 4.952
Grimsley 4.914
Solomon 5.041
Lehninger 4.991
Nozaki 5.169
DTASelect 5.321
Thurlkill 5.003
EMBOSS 4.965
Sillero 5.232
Patrickios 3.973
IPC_peptide 5.041
IPC2_peptide 5.232
IPC2.peptide.svr19 5.206
Protein with the highest isoelectric point:
>sp|O39832|U2_FBNY2 Protein U2 OS=Faba bean necrotic yellows virus (isolate SV292-88) OX=291604 GN=DNA-U2 PE=3 SV=1
MM1 pKa = 7.37 ASKK4 pKa = 10.16 WNWSGTKK11 pKa = 9.85 GRR13 pKa = 11.84 RR14 pKa = 11.84 TPRR17 pKa = 11.84 RR18 pKa = 11.84 PYY20 pKa = 9.37 GRR22 pKa = 11.84 PYY24 pKa = 10.15 KK25 pKa = 10.65 SSVPTTRR32 pKa = 11.84 VVVHH36 pKa = 6.05 QSAVLKK42 pKa = 10.21 KK43 pKa = 10.18 DD44 pKa = 3.39 EE45 pKa = 4.34 VVGTEE50 pKa = 4.23 IKK52 pKa = 10.71 PEE54 pKa = 4.08 GDD56 pKa = 3.06 VARR59 pKa = 11.84 YY60 pKa = 9.51 KK61 pKa = 9.79 MKK63 pKa = 10.69 KK64 pKa = 10.34 VMLTCTLRR72 pKa = 11.84 MAPGEE77 pKa = 4.04 LVNYY81 pKa = 9.56 LIVKK85 pKa = 9.1 CNSPISSWSAAFTSPALLVKK105 pKa = 9.94 EE106 pKa = 4.7 SCQDD110 pKa = 3.44 MITIIAKK117 pKa = 10.31 GKK119 pKa = 9.81 VEE121 pKa = 4.21 STGVAGTDD129 pKa = 3.39 CTKK132 pKa = 10.72 SFNRR136 pKa = 11.84 FIKK139 pKa = 10.4 LGAGITQTQHH149 pKa = 6.85 LYY151 pKa = 9.5 VVLYY155 pKa = 9.51 TSVALKK161 pKa = 10.41 AVLEE165 pKa = 4.06 HH166 pKa = 6.11 RR167 pKa = 11.84 VYY169 pKa = 11.44 VEE171 pKa = 3.73 VV172 pKa = 3.87
Molecular weight: 19.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.118
IPC2_protein 9.268
IPC_protein 9.194
Toseland 9.984
ProMoST 9.633
Dawson 10.175
Bjellqvist 9.838
Wikipedia 10.321
Rodwell 10.672
Grimsley 10.233
Solomon 10.204
Lehninger 10.175
Nozaki 10.028
DTASelect 9.823
Thurlkill 10.028
EMBOSS 10.379
Sillero 10.101
Patrickios 10.292
IPC_peptide 10.204
IPC2_peptide 8.551
IPC2.peptide.svr19 8.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
2263
65
286
174.1
20.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.242 ± 0.583
2.563 ± 0.373
5.524 ± 0.572
7.424 ± 0.701
3.403 ± 0.278
5.391 ± 0.555
2.386 ± 0.277
6.186 ± 0.454
7.689 ± 0.468
8.794 ± 0.838
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.27 ± 0.246
4.419 ± 0.465
3.889 ± 0.309
3.756 ± 0.24
5.833 ± 0.452
7.159 ± 0.702
4.331 ± 0.571
6.673 ± 0.636
1.9 ± 0.305
5.17 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here