Faba bean necrotic yellows virus (isolate SV292-88) (FBNYV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Mulpavirales; Nanoviridae; Nanovirus; Faba bean necrotic yellows virus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|P0CK61|REP9_FBNY2 Para-Rep C9 OS=Faba bean necrotic yellows virus (isolate SV292-88) OX=291604 GN=C9 PE=3 SV=1
MM1 pKa = 6.85GLKK4 pKa = 10.18YY5 pKa = 10.39FSHH8 pKa = 7.43LPEE11 pKa = 4.26EE12 pKa = 4.28LRR14 pKa = 11.84QKK16 pKa = 10.02IVHH19 pKa = 6.74DD20 pKa = 3.96HH21 pKa = 6.09LQQEE25 pKa = 4.51RR26 pKa = 11.84KK27 pKa = 9.8KK28 pKa = 10.67EE29 pKa = 3.93FLEE32 pKa = 4.07KK33 pKa = 10.36AIEE36 pKa = 4.35DD37 pKa = 3.56SCRR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 5.71VSLLKK47 pKa = 10.29SDD49 pKa = 4.59PSPSEE54 pKa = 4.52LYY56 pKa = 10.79ALSKK60 pKa = 10.73FLDD63 pKa = 3.59SLADD67 pKa = 3.67YY68 pKa = 10.66VGKK71 pKa = 10.24QFNTRR76 pKa = 11.84CLIKK80 pKa = 9.87WKK82 pKa = 10.34KK83 pKa = 10.13DD84 pKa = 3.02VPANIKK90 pKa = 10.34FEE92 pKa = 4.23VMEE95 pKa = 4.24EE96 pKa = 3.77QHH98 pKa = 6.08LRR100 pKa = 11.84LYY102 pKa = 11.17GFVDD106 pKa = 4.03MDD108 pKa = 4.22DD109 pKa = 3.83LLCRR113 pKa = 11.84EE114 pKa = 4.26VLPPEE119 pKa = 4.33EE120 pKa = 5.79DD121 pKa = 4.23DD122 pKa = 5.7DD123 pKa = 3.85ITYY126 pKa = 10.57EE127 pKa = 4.33DD128 pKa = 4.43GMIVNCSEE136 pKa = 3.96LDD138 pKa = 3.52KK139 pKa = 11.35LFEE142 pKa = 4.25ALGIKK147 pKa = 9.35VVYY150 pKa = 9.0ITVSKK155 pKa = 11.24NCICTPLNKK164 pKa = 10.32DD165 pKa = 3.1IVISS169 pKa = 3.83

Molecular weight:
19.68 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|O39832|U2_FBNY2 Protein U2 OS=Faba bean necrotic yellows virus (isolate SV292-88) OX=291604 GN=DNA-U2 PE=3 SV=1
MM1 pKa = 7.37ASKK4 pKa = 10.16WNWSGTKK11 pKa = 9.85GRR13 pKa = 11.84RR14 pKa = 11.84TPRR17 pKa = 11.84RR18 pKa = 11.84PYY20 pKa = 9.37GRR22 pKa = 11.84PYY24 pKa = 10.15KK25 pKa = 10.65SSVPTTRR32 pKa = 11.84VVVHH36 pKa = 6.05QSAVLKK42 pKa = 10.21KK43 pKa = 10.18DD44 pKa = 3.39EE45 pKa = 4.34VVGTEE50 pKa = 4.23IKK52 pKa = 10.71PEE54 pKa = 4.08GDD56 pKa = 3.06VARR59 pKa = 11.84YY60 pKa = 9.51KK61 pKa = 9.79MKK63 pKa = 10.69KK64 pKa = 10.34VMLTCTLRR72 pKa = 11.84MAPGEE77 pKa = 4.04LVNYY81 pKa = 9.56LIVKK85 pKa = 9.1CNSPISSWSAAFTSPALLVKK105 pKa = 9.94EE106 pKa = 4.7SCQDD110 pKa = 3.44MITIIAKK117 pKa = 10.31GKK119 pKa = 9.81VEE121 pKa = 4.21STGVAGTDD129 pKa = 3.39CTKK132 pKa = 10.72SFNRR136 pKa = 11.84FIKK139 pKa = 10.4LGAGITQTQHH149 pKa = 6.85LYY151 pKa = 9.5VVLYY155 pKa = 9.51TSVALKK161 pKa = 10.41AVLEE165 pKa = 4.06HH166 pKa = 6.11RR167 pKa = 11.84VYY169 pKa = 11.44VEE171 pKa = 3.73VV172 pKa = 3.87

Molecular weight:
19.05 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

2263

65

286

174.1

20.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.242 ± 0.583

2.563 ± 0.373

5.524 ± 0.572

7.424 ± 0.701

3.403 ± 0.278

5.391 ± 0.555

2.386 ± 0.277

6.186 ± 0.454

7.689 ± 0.468

8.794 ± 0.838

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.27 ± 0.246

4.419 ± 0.465

3.889 ± 0.309

3.756 ± 0.24

5.833 ± 0.452

7.159 ± 0.702

4.331 ± 0.571

6.673 ± 0.636

1.9 ± 0.305

5.17 ± 0.371

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski