Gordonia soli NBRC 108243
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4907 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M0QIR8|M0QIR8_9ACTN Methionine import ATP-binding protein MetN OS=Gordonia soli NBRC 108243 OX=1223545 GN=metN PE=3 SV=1
MM1 pKa = 7.7 TDD3 pKa = 2.96 VFDD6 pKa = 5.69 GDD8 pKa = 3.92 RR9 pKa = 11.84 LLQAVDD15 pKa = 3.38 VARR18 pKa = 11.84 AALIDD23 pKa = 5.07 DD24 pKa = 4.75 GQQPGAHH31 pKa = 6.83 LDD33 pKa = 3.49 AGVEE37 pKa = 4.15 GDD39 pKa = 3.54 WAVAHH44 pKa = 6.03 YY45 pKa = 8.73 FAADD49 pKa = 3.02 IPGYY53 pKa = 10.19 RR54 pKa = 11.84 GWQWCVVLSGAPGSDD69 pKa = 3.91 EE70 pKa = 3.86 ITVSEE75 pKa = 4.47 VVLLPGEE82 pKa = 4.56 GSLLAPTWVPWIEE95 pKa = 3.83 RR96 pKa = 11.84 VAAGDD101 pKa = 4.2 LGPGDD106 pKa = 4.56 VLAADD111 pKa = 4.46 PDD113 pKa = 4.18 DD114 pKa = 3.97 VRR116 pKa = 11.84 LVPNQIDD123 pKa = 3.91 TEE125 pKa = 4.69 DD126 pKa = 4.14 EE127 pKa = 4.08 FHH129 pKa = 7.52 LDD131 pKa = 3.94 PDD133 pKa = 4.94 DD134 pKa = 6.62 DD135 pKa = 4.52 DD136 pKa = 6.34 PEE138 pKa = 7.16 DD139 pKa = 3.41 IGQIAGEE146 pKa = 4.16 LGLGRR151 pKa = 11.84 RR152 pKa = 11.84 RR153 pKa = 11.84 LLSPEE158 pKa = 3.99 GRR160 pKa = 11.84 DD161 pKa = 3.07 EE162 pKa = 4.48 AAQRR166 pKa = 11.84 WFDD169 pKa = 3.54 GDD171 pKa = 4.18 FGPASEE177 pKa = 4.5 MAQAAPYY184 pKa = 8.37 PCCTCGFYY192 pKa = 11.12 VPLGGALRR200 pKa = 11.84 PAFGACANEE209 pKa = 3.94 YY210 pKa = 10.33 AADD213 pKa = 3.89 GRR215 pKa = 11.84 VVAADD220 pKa = 3.87 YY221 pKa = 11.38 GCGAHH226 pKa = 6.99 SDD228 pKa = 3.68 VAPPKK233 pKa = 10.45 GGEE236 pKa = 3.94 GSPAYY241 pKa = 9.91 DD242 pKa = 4.15 AYY244 pKa = 11.12 DD245 pKa = 4.21 DD246 pKa = 4.24 GAVEE250 pKa = 4.2 IVEE253 pKa = 4.29 ITPEE257 pKa = 4.13 PATVGADD264 pKa = 3.31 ADD266 pKa = 4.19 GSS268 pKa = 3.93
Molecular weight: 27.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.694
IPC_protein 3.732
Toseland 3.49
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.101
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.771
Protein with the highest isoelectric point:
>tr|M0QH84|M0QH84_9ACTN Putative fatty-acid--CoA ligase OS=Gordonia soli NBRC 108243 OX=1223545 GN=GS4_11_01570 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.99 GFRR22 pKa = 11.84 LRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVNGRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 10.03 GRR42 pKa = 11.84 EE43 pKa = 3.54 KK44 pKa = 10.3 LTAA47 pKa = 4.1
Molecular weight: 5.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.468
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.398
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.135
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.122
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4907
0
4907
1606877
40
7062
327.5
35.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.833 ± 0.043
0.646 ± 0.01
6.933 ± 0.034
5.151 ± 0.031
2.93 ± 0.019
9.08 ± 0.034
2.152 ± 0.016
4.52 ± 0.024
1.874 ± 0.028
9.389 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.811 ± 0.014
1.966 ± 0.021
5.648 ± 0.026
2.67 ± 0.018
7.601 ± 0.036
5.874 ± 0.026
6.465 ± 0.023
9.058 ± 0.036
1.442 ± 0.013
1.958 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here