Williamsia sp. D3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Williamsia; unclassified Williamsia

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4926 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V8D0L1|V8D0L1_9NOCA Pyridoxine 5'-phosphate oxidase OS=Williamsia sp. D3 OX=1313067 GN=W823_11090 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.34SDD4 pKa = 3.48VVRR7 pKa = 11.84VLVCVAAVGAAVSAGAGAASAAPTDD32 pKa = 3.43TCTAADD38 pKa = 4.04RR39 pKa = 11.84VAGAQTYY46 pKa = 11.04LDD48 pKa = 3.93ALVDD52 pKa = 3.51PSYY55 pKa = 11.44AYY57 pKa = 9.91DD58 pKa = 3.63VPLADD63 pKa = 3.86DD64 pKa = 3.56VVRR67 pKa = 11.84FEE69 pKa = 5.45NGLQTGFSGDD79 pKa = 3.27VMKK82 pKa = 11.21AEE84 pKa = 4.52LALHH88 pKa = 6.07LQYY91 pKa = 11.27SGITSLDD98 pKa = 3.4NEE100 pKa = 4.31QWSGNGDD107 pKa = 4.07SVRR110 pKa = 11.84VIYY113 pKa = 10.46DD114 pKa = 3.27LEE116 pKa = 4.6YY117 pKa = 11.13GVGDD121 pKa = 4.85LNLADD126 pKa = 3.7ATVDD130 pKa = 2.99EE131 pKa = 4.76TFRR134 pKa = 11.84FNADD138 pKa = 3.51CLIQRR143 pKa = 11.84IDD145 pKa = 3.45ATITIAPGDD154 pKa = 3.88GFF156 pKa = 4.15

Molecular weight: 16.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V8CUU1|V8CUU1_9NOCA Peptidase S8 OS=Williamsia sp. D3 OX=1313067 GN=W823_22555 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4926

0

4926

1498476

29

6905

304.2

32.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.283 ± 0.041

0.769 ± 0.01

6.509 ± 0.029

5.394 ± 0.028

3.005 ± 0.022

8.835 ± 0.03

2.164 ± 0.019

4.621 ± 0.023

2.363 ± 0.026

9.599 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.133 ± 0.014

2.287 ± 0.017

5.531 ± 0.026

3.043 ± 0.021

6.95 ± 0.036

5.974 ± 0.023

6.279 ± 0.022

8.76 ± 0.032

1.467 ± 0.013

2.036 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski