Amino acid dipepetide frequency for Aeromonas phage pAh6-C

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.824AlaAla: 8.824 ± 1.137
0.926AlaCys: 0.926 ± 0.224
4.813AlaAsp: 4.813 ± 0.551
5.184AlaGlu: 5.184 ± 0.561
3.024AlaPhe: 3.024 ± 0.347
7.837AlaGly: 7.837 ± 0.694
1.419AlaHis: 1.419 ± 0.276
5.307AlaIle: 5.307 ± 0.516
5.43AlaLys: 5.43 ± 0.602
7.097AlaLeu: 7.097 ± 0.857
2.777AlaMet: 2.777 ± 0.408
2.715AlaAsn: 2.715 ± 0.396
4.073AlaPro: 4.073 ± 0.542
4.875AlaGln: 4.875 ± 0.603
4.32AlaArg: 4.32 ± 0.586
4.073AlaSer: 4.073 ± 0.569
5.184AlaThr: 5.184 ± 0.614
6.85AlaVal: 6.85 ± 0.734
0.926AlaTrp: 0.926 ± 0.257
2.592AlaTyr: 2.592 ± 0.345
0.0AlaXaa: 0.0 ± 0.0
Cys
0.432CysAla: 0.432 ± 0.172
0.123CysCys: 0.123 ± 0.104
0.432CysAsp: 0.432 ± 0.141
0.987CysGlu: 0.987 ± 0.202
0.555CysPhe: 0.555 ± 0.206
1.296CysGly: 1.296 ± 0.305
0.432CysHis: 0.432 ± 0.174
0.802CysIle: 0.802 ± 0.22
0.679CysLys: 0.679 ± 0.232
1.049CysLeu: 1.049 ± 0.25
0.494CysMet: 0.494 ± 0.145
0.802CysAsn: 0.802 ± 0.221
0.432CysPro: 0.432 ± 0.163
0.494CysGln: 0.494 ± 0.175
0.864CysArg: 0.864 ± 0.223
0.864CysSer: 0.864 ± 0.236
0.555CysThr: 0.555 ± 0.251
0.555CysVal: 0.555 ± 0.16
0.247CysTrp: 0.247 ± 0.166
0.494CysTyr: 0.494 ± 0.166
0.0CysXaa: 0.0 ± 0.0
Asp
6.541AspAla: 6.541 ± 0.826
0.555AspCys: 0.555 ± 0.199
3.703AspAsp: 3.703 ± 0.663
3.394AspGlu: 3.394 ± 0.532
2.098AspPhe: 2.098 ± 0.354
4.998AspGly: 4.998 ± 0.529
1.419AspHis: 1.419 ± 0.226
3.579AspIle: 3.579 ± 0.441
3.517AspLys: 3.517 ± 0.493
5.554AspLeu: 5.554 ± 0.527
1.79AspMet: 1.79 ± 0.348
2.407AspAsn: 2.407 ± 0.377
3.271AspPro: 3.271 ± 0.576
1.543AspGln: 1.543 ± 0.31
2.407AspArg: 2.407 ± 0.391
2.715AspSer: 2.715 ± 0.461
3.209AspThr: 3.209 ± 0.459
3.703AspVal: 3.703 ± 0.516
1.111AspTrp: 1.111 ± 0.224
2.345AspTyr: 2.345 ± 0.382
0.0AspXaa: 0.0 ± 0.0
Glu
6.171GluAla: 6.171 ± 0.758
0.617GluCys: 0.617 ± 0.176
3.641GluAsp: 3.641 ± 0.544
4.998GluGlu: 4.998 ± 0.714
1.79GluPhe: 1.79 ± 0.386
4.381GluGly: 4.381 ± 0.502
1.419GluHis: 1.419 ± 0.304
4.32GluIle: 4.32 ± 0.508
3.209GluLys: 3.209 ± 0.505
5.986GluLeu: 5.986 ± 0.68
2.098GluMet: 2.098 ± 0.314
1.666GluAsn: 1.666 ± 0.327
2.098GluPro: 2.098 ± 0.31
2.962GluGln: 2.962 ± 0.464
2.839GluArg: 2.839 ± 0.461
3.394GluSer: 3.394 ± 0.437
2.777GluThr: 2.777 ± 0.381
4.443GluVal: 4.443 ± 0.601
1.172GluTrp: 1.172 ± 0.301
2.407GluTyr: 2.407 ± 0.39
0.0GluXaa: 0.0 ± 0.0
Phe
2.407PheAla: 2.407 ± 0.516
0.617PheCys: 0.617 ± 0.18
2.53PheAsp: 2.53 ± 0.371
3.085PheGlu: 3.085 ± 0.489
1.111PhePhe: 1.111 ± 0.265
1.975PheGly: 1.975 ± 0.318
0.926PheHis: 0.926 ± 0.214
1.79PheIle: 1.79 ± 0.446
2.16PheLys: 2.16 ± 0.43
2.283PheLeu: 2.283 ± 0.315
1.358PheMet: 1.358 ± 0.27
2.283PheAsn: 2.283 ± 0.321
1.419PhePro: 1.419 ± 0.314
0.987PheGln: 0.987 ± 0.195
1.851PheArg: 1.851 ± 0.332
2.839PheSer: 2.839 ± 0.36
1.851PheThr: 1.851 ± 0.259
2.592PheVal: 2.592 ± 0.429
0.679PheTrp: 0.679 ± 0.195
0.679PheTyr: 0.679 ± 0.21
0.0PheXaa: 0.0 ± 0.0
Gly
6.603GlyAla: 6.603 ± 0.776
0.987GlyCys: 0.987 ± 0.217
4.381GlyAsp: 4.381 ± 0.527
4.258GlyGlu: 4.258 ± 0.428
3.147GlyPhe: 3.147 ± 0.353
6.233GlyGly: 6.233 ± 0.961
1.296GlyHis: 1.296 ± 0.346
6.109GlyIle: 6.109 ± 0.647
4.998GlyLys: 4.998 ± 0.562
5.369GlyLeu: 5.369 ± 0.709
2.16GlyMet: 2.16 ± 0.309
2.654GlyAsn: 2.654 ± 0.564
1.604GlyPro: 1.604 ± 0.306
2.9GlyGln: 2.9 ± 0.343
4.505GlyArg: 4.505 ± 0.594
4.32GlySer: 4.32 ± 0.63
4.998GlyThr: 4.998 ± 0.749
5.369GlyVal: 5.369 ± 0.608
1.358GlyTrp: 1.358 ± 0.31
2.839GlyTyr: 2.839 ± 0.486
0.0GlyXaa: 0.0 ± 0.0
His
1.79HisAla: 1.79 ± 0.31
0.432HisCys: 0.432 ± 0.156
1.111HisAsp: 1.111 ± 0.266
2.036HisGlu: 2.036 ± 0.349
0.555HisPhe: 0.555 ± 0.21
1.481HisGly: 1.481 ± 0.264
0.247HisHis: 0.247 ± 0.137
1.481HisIle: 1.481 ± 0.277
1.296HisLys: 1.296 ± 0.294
1.604HisLeu: 1.604 ± 0.355
0.617HisMet: 0.617 ± 0.201
0.802HisAsn: 0.802 ± 0.241
1.481HisPro: 1.481 ± 0.358
0.741HisGln: 0.741 ± 0.168
0.987HisArg: 0.987 ± 0.189
1.049HisSer: 1.049 ± 0.297
1.296HisThr: 1.296 ± 0.253
1.666HisVal: 1.666 ± 0.393
0.37HisTrp: 0.37 ± 0.146
0.987HisTyr: 0.987 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
5.492IleAla: 5.492 ± 0.664
0.617IleCys: 0.617 ± 0.183
3.517IleAsp: 3.517 ± 0.43
3.764IleGlu: 3.764 ± 0.512
1.975IlePhe: 1.975 ± 0.385
3.579IleGly: 3.579 ± 0.488
1.296IleHis: 1.296 ± 0.236
1.913IleIle: 1.913 ± 0.365
4.011IleLys: 4.011 ± 0.444
3.456IleLeu: 3.456 ± 0.395
1.728IleMet: 1.728 ± 0.367
3.209IleAsn: 3.209 ± 0.37
3.024IlePro: 3.024 ± 0.353
1.975IleGln: 1.975 ± 0.338
3.271IleArg: 3.271 ± 0.419
3.147IleSer: 3.147 ± 0.394
4.998IleThr: 4.998 ± 0.653
3.764IleVal: 3.764 ± 0.483
0.679IleTrp: 0.679 ± 0.222
1.296IleTyr: 1.296 ± 0.332
0.0IleXaa: 0.0 ± 0.0
Lys
4.998LysAla: 4.998 ± 0.688
0.802LysCys: 0.802 ± 0.213
3.209LysAsp: 3.209 ± 0.496
2.839LysGlu: 2.839 ± 0.472
2.16LysPhe: 2.16 ± 0.299
4.135LysGly: 4.135 ± 0.452
1.604LysHis: 1.604 ± 0.255
2.9LysIle: 2.9 ± 0.503
2.715LysLys: 2.715 ± 0.394
5.06LysLeu: 5.06 ± 0.548
2.222LysMet: 2.222 ± 0.426
2.036LysAsn: 2.036 ± 0.37
2.53LysPro: 2.53 ± 0.469
1.975LysGln: 1.975 ± 0.384
2.407LysArg: 2.407 ± 0.454
3.641LysSer: 3.641 ± 0.489
4.011LysThr: 4.011 ± 0.555
4.135LysVal: 4.135 ± 0.506
0.741LysTrp: 0.741 ± 0.181
2.468LysTyr: 2.468 ± 0.421
0.0LysXaa: 0.0 ± 0.0
Leu
6.479LeuAla: 6.479 ± 0.649
0.926LeuCys: 0.926 ± 0.227
4.381LeuAsp: 4.381 ± 0.586
5.554LeuGlu: 5.554 ± 0.655
2.036LeuPhe: 2.036 ± 0.395
5.801LeuGly: 5.801 ± 0.61
1.481LeuHis: 1.481 ± 0.288
4.258LeuIle: 4.258 ± 0.44
4.443LeuLys: 4.443 ± 0.528
5.184LeuLeu: 5.184 ± 0.698
2.715LeuMet: 2.715 ± 0.378
3.579LeuAsn: 3.579 ± 0.384
3.949LeuPro: 3.949 ± 0.494
3.888LeuGln: 3.888 ± 0.522
5.245LeuArg: 5.245 ± 0.657
4.258LeuSer: 4.258 ± 0.5
4.998LeuThr: 4.998 ± 0.538
4.32LeuVal: 4.32 ± 0.437
1.296LeuTrp: 1.296 ± 0.227
2.592LeuTyr: 2.592 ± 0.537
0.0LeuXaa: 0.0 ± 0.0
Met
3.085MetAla: 3.085 ± 0.449
0.555MetCys: 0.555 ± 0.168
1.666MetAsp: 1.666 ± 0.313
1.543MetGlu: 1.543 ± 0.354
0.802MetPhe: 0.802 ± 0.215
1.975MetGly: 1.975 ± 0.364
1.049MetHis: 1.049 ± 0.206
1.481MetIle: 1.481 ± 0.286
1.666MetLys: 1.666 ± 0.362
2.283MetLeu: 2.283 ± 0.331
0.926MetMet: 0.926 ± 0.271
1.172MetAsn: 1.172 ± 0.218
1.481MetPro: 1.481 ± 0.318
2.036MetGln: 2.036 ± 0.346
1.728MetArg: 1.728 ± 0.303
2.839MetSer: 2.839 ± 0.473
2.468MetThr: 2.468 ± 0.408
1.975MetVal: 1.975 ± 0.282
0.309MetTrp: 0.309 ± 0.123
0.926MetTyr: 0.926 ± 0.193
0.0MetXaa: 0.0 ± 0.0
Asn
2.962AsnAla: 2.962 ± 0.395
0.555AsnCys: 0.555 ± 0.199
2.036AsnAsp: 2.036 ± 0.384
2.283AsnGlu: 2.283 ± 0.439
1.913AsnPhe: 1.913 ± 0.327
4.752AsnGly: 4.752 ± 0.482
0.987AsnHis: 0.987 ± 0.269
2.468AsnIle: 2.468 ± 0.449
2.036AsnLys: 2.036 ± 0.288
3.147AsnLeu: 3.147 ± 0.386
0.679AsnMet: 0.679 ± 0.236
1.543AsnAsn: 1.543 ± 0.333
3.085AsnPro: 3.085 ± 0.423
1.913AsnGln: 1.913 ± 0.429
2.098AsnArg: 2.098 ± 0.308
2.777AsnSer: 2.777 ± 0.415
3.147AsnThr: 3.147 ± 0.387
2.654AsnVal: 2.654 ± 0.332
0.617AsnTrp: 0.617 ± 0.189
1.234AsnTyr: 1.234 ± 0.231
0.0AsnXaa: 0.0 ± 0.0
Pro
4.381ProAla: 4.381 ± 0.528
0.309ProCys: 0.309 ± 0.126
2.9ProAsp: 2.9 ± 0.465
2.962ProGlu: 2.962 ± 0.417
1.358ProPhe: 1.358 ± 0.324
2.839ProGly: 2.839 ± 0.376
1.111ProHis: 1.111 ± 0.331
1.728ProIle: 1.728 ± 0.355
2.222ProLys: 2.222 ± 0.397
2.9ProLeu: 2.9 ± 0.438
1.419ProMet: 1.419 ± 0.295
2.283ProAsn: 2.283 ± 0.301
1.234ProPro: 1.234 ± 0.324
1.851ProGln: 1.851 ± 0.306
2.468ProArg: 2.468 ± 0.375
2.345ProSer: 2.345 ± 0.356
2.592ProThr: 2.592 ± 0.393
4.073ProVal: 4.073 ± 0.553
0.309ProTrp: 0.309 ± 0.155
1.913ProTyr: 1.913 ± 0.362
0.0ProXaa: 0.0 ± 0.0
Gln
3.826GlnAla: 3.826 ± 0.554
0.555GlnCys: 0.555 ± 0.148
2.468GlnAsp: 2.468 ± 0.303
3.085GlnGlu: 3.085 ± 0.47
1.666GlnPhe: 1.666 ± 0.287
2.592GlnGly: 2.592 ± 0.458
0.926GlnHis: 0.926 ± 0.235
1.975GlnIle: 1.975 ± 0.312
2.16GlnLys: 2.16 ± 0.406
3.332GlnLeu: 3.332 ± 0.515
1.666GlnMet: 1.666 ± 0.346
1.604GlnAsn: 1.604 ± 0.312
1.172GlnPro: 1.172 ± 0.235
2.53GlnGln: 2.53 ± 0.663
2.777GlnArg: 2.777 ± 0.457
2.222GlnSer: 2.222 ± 0.409
2.098GlnThr: 2.098 ± 0.34
2.962GlnVal: 2.962 ± 0.417
0.864GlnTrp: 0.864 ± 0.225
1.851GlnTyr: 1.851 ± 0.33
0.0GlnXaa: 0.0 ± 0.0
Arg
4.628ArgAla: 4.628 ± 0.397
0.617ArgCys: 0.617 ± 0.252
3.271ArgAsp: 3.271 ± 0.383
3.209ArgGlu: 3.209 ± 0.47
2.098ArgPhe: 2.098 ± 0.295
3.826ArgGly: 3.826 ± 0.629
1.296ArgHis: 1.296 ± 0.265
2.962ArgIle: 2.962 ± 0.432
2.962ArgLys: 2.962 ± 0.515
4.505ArgLeu: 4.505 ± 0.48
2.036ArgMet: 2.036 ± 0.344
2.777ArgAsn: 2.777 ± 0.443
1.604ArgPro: 1.604 ± 0.389
2.222ArgGln: 2.222 ± 0.362
3.271ArgArg: 3.271 ± 0.506
3.517ArgSer: 3.517 ± 0.416
2.839ArgThr: 2.839 ± 0.474
3.826ArgVal: 3.826 ± 0.557
0.555ArgTrp: 0.555 ± 0.168
2.098ArgTyr: 2.098 ± 0.4
0.0ArgXaa: 0.0 ± 0.0
Ser
4.69SerAla: 4.69 ± 0.667
0.987SerCys: 0.987 ± 0.216
3.147SerAsp: 3.147 ± 0.465
3.517SerGlu: 3.517 ± 0.55
2.592SerPhe: 2.592 ± 0.416
5.43SerGly: 5.43 ± 0.693
1.296SerHis: 1.296 ± 0.288
3.394SerIle: 3.394 ± 0.413
2.9SerLys: 2.9 ± 0.408
5.245SerLeu: 5.245 ± 0.558
1.234SerMet: 1.234 ± 0.236
2.654SerAsn: 2.654 ± 0.376
1.666SerPro: 1.666 ± 0.325
2.592SerGln: 2.592 ± 0.346
3.517SerArg: 3.517 ± 0.42
3.085SerSer: 3.085 ± 0.452
3.271SerThr: 3.271 ± 0.576
4.196SerVal: 4.196 ± 0.462
0.926SerTrp: 0.926 ± 0.254
2.036SerTyr: 2.036 ± 0.376
0.0SerXaa: 0.0 ± 0.0
Thr
5.245ThrAla: 5.245 ± 0.662
0.494ThrCys: 0.494 ± 0.28
4.073ThrAsp: 4.073 ± 0.612
2.345ThrGlu: 2.345 ± 0.349
2.345ThrPhe: 2.345 ± 0.325
5.492ThrGly: 5.492 ± 0.606
1.358ThrHis: 1.358 ± 0.257
3.641ThrIle: 3.641 ± 0.525
3.456ThrLys: 3.456 ± 0.412
4.937ThrLeu: 4.937 ± 0.6
1.79ThrMet: 1.79 ± 0.4
3.271ThrAsn: 3.271 ± 0.465
3.271ThrPro: 3.271 ± 0.401
1.728ThrGln: 1.728 ± 0.305
3.332ThrArg: 3.332 ± 0.403
3.147ThrSer: 3.147 ± 0.437
3.949ThrThr: 3.949 ± 0.563
4.628ThrVal: 4.628 ± 0.644
0.679ThrTrp: 0.679 ± 0.233
1.666ThrTyr: 1.666 ± 0.381
0.0ThrXaa: 0.0 ± 0.0
Val
6.048ValAla: 6.048 ± 0.578
0.864ValCys: 0.864 ± 0.2
5.307ValAsp: 5.307 ± 0.521
4.381ValGlu: 4.381 ± 0.528
2.592ValPhe: 2.592 ± 0.378
3.949ValGly: 3.949 ± 0.547
1.111ValHis: 1.111 ± 0.305
4.011ValIle: 4.011 ± 0.526
4.443ValLys: 4.443 ± 0.703
4.813ValLeu: 4.813 ± 0.5
2.468ValMet: 2.468 ± 0.418
2.9ValAsn: 2.9 ± 0.49
3.764ValPro: 3.764 ± 0.522
2.962ValGln: 2.962 ± 0.386
4.32ValArg: 4.32 ± 0.522
4.32ValSer: 4.32 ± 0.479
3.826ValThr: 3.826 ± 0.492
6.665ValVal: 6.665 ± 0.77
0.741ValTrp: 0.741 ± 0.216
1.666ValTyr: 1.666 ± 0.315
0.0ValXaa: 0.0 ± 0.0
Trp
1.296TrpAla: 1.296 ± 0.338
0.37TrpCys: 0.37 ± 0.134
0.679TrpAsp: 0.679 ± 0.255
1.172TrpGlu: 1.172 ± 0.316
0.555TrpPhe: 0.555 ± 0.179
0.679TrpGly: 0.679 ± 0.172
0.247TrpHis: 0.247 ± 0.111
0.679TrpIle: 0.679 ± 0.195
0.617TrpLys: 0.617 ± 0.26
1.296TrpLeu: 1.296 ± 0.252
0.617TrpMet: 0.617 ± 0.168
0.802TrpAsn: 0.802 ± 0.211
0.309TrpPro: 0.309 ± 0.135
0.679TrpGln: 0.679 ± 0.189
0.679TrpArg: 0.679 ± 0.209
0.494TrpSer: 0.494 ± 0.189
0.741TrpThr: 0.741 ± 0.204
1.049TrpVal: 1.049 ± 0.242
0.185TrpTrp: 0.185 ± 0.098
0.802TrpTyr: 0.802 ± 0.193
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.407TyrAla: 2.407 ± 0.476
0.679TyrCys: 0.679 ± 0.212
2.592TyrAsp: 2.592 ± 0.488
1.79TyrGlu: 1.79 ± 0.423
1.049TyrPhe: 1.049 ± 0.276
2.839TyrGly: 2.839 ± 0.509
1.111TyrHis: 1.111 ± 0.324
1.913TyrIle: 1.913 ± 0.364
1.666TyrLys: 1.666 ± 0.354
2.283TyrLeu: 2.283 ± 0.411
1.111TyrMet: 1.111 ± 0.267
1.728TyrAsn: 1.728 ± 0.347
1.728TyrPro: 1.728 ± 0.305
1.481TyrGln: 1.481 ± 0.356
1.234TyrArg: 1.234 ± 0.29
3.332TyrSer: 3.332 ± 0.511
2.036TyrThr: 2.036 ± 0.373
1.728TyrVal: 1.728 ± 0.388
0.185TyrTrp: 0.185 ± 0.093
0.987TyrTyr: 0.987 ± 0.262
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (16206 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski