Amino acid dipepetide frequency for Bacillus sporothermodurans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.697AlaAla: 4.697 ± 0.079	0.501AlaCys: 0.501 ± 0.026	2.884AlaAsp: 2.884 ± 0.058	3.994AlaGlu: 3.994 ± 0.068	2.915AlaPhe: 2.915 ± 0.047	4.476AlaGly: 4.476 ± 0.087	1.107AlaHis: 1.107 ± 0.032	5.905AlaIle: 5.905 ± 0.081	4.994AlaLys: 4.994 ± 0.068	6.207AlaLeu: 6.207 ± 0.075	1.749AlaMet: 1.749 ± 0.034	2.999AlaAsn: 2.999 ± 0.058	1.883AlaPro: 1.883 ± 0.048	1.895AlaGln: 1.895 ± 0.046	2.314AlaArg: 2.314 ± 0.045	3.717AlaSer: 3.717 ± 0.07	3.24AlaThr: 3.24 ± 0.061	4.355AlaVal: 4.355 ± 0.065	0.552AlaTrp: 0.552 ± 0.024	2.142AlaTyr: 2.142 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	0.417CysAla: 0.417 ± 0.02	0.092CysCys: 0.092 ± 0.01	0.386CysAsp: 0.386 ± 0.019	0.501CysGlu: 0.501 ± 0.022	0.351CysPhe: 0.351 ± 0.016	0.666CysGly: 0.666 ± 0.027	0.193CysHis: 0.193 ± 0.013	0.606CysIle: 0.606 ± 0.022	0.427CysLys: 0.427 ± 0.022	0.698CysLeu: 0.698 ± 0.025	0.198CysMet: 0.198 ± 0.014	0.305CysAsn: 0.305 ± 0.019	0.337CysPro: 0.337 ± 0.016	0.252CysGln: 0.252 ± 0.014	0.247CysArg: 0.247 ± 0.014	0.507CysSer: 0.507 ± 0.021	0.379CysThr: 0.379 ± 0.019	0.38CysVal: 0.38 ± 0.02	0.08CysTrp: 0.08 ± 0.008	0.259CysTyr: 0.259 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	2.976AspAla: 2.976 ± 0.058	0.387AspCys: 0.387 ± 0.019	2.319AspAsp: 2.319 ± 0.055	4.38AspGlu: 4.38 ± 0.065	2.55AspPhe: 2.55 ± 0.056	3.149AspGly: 3.149 ± 0.053	1.101AspHis: 1.101 ± 0.035	4.466AspIle: 4.466 ± 0.059	3.519AspLys: 3.519 ± 0.065	4.833AspLeu: 4.833 ± 0.057	1.207AspMet: 1.207 ± 0.033	1.936AspAsn: 1.936 ± 0.048	1.95AspPro: 1.95 ± 0.045	2.113AspGln: 2.113 ± 0.049	2.067AspArg: 2.067 ± 0.049	2.654AspSer: 2.654 ± 0.049	2.307AspThr: 2.307 ± 0.05	3.533AspVal: 3.533 ± 0.06	0.644AspTrp: 0.644 ± 0.023	2.242AspTyr: 2.242 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	4.523GluAla: 4.523 ± 0.077	0.397GluCys: 0.397 ± 0.018	3.513GluAsp: 3.513 ± 0.064	6.42GluGlu: 6.42 ± 0.097	2.693GluPhe: 2.693 ± 0.051	3.803GluGly: 3.803 ± 0.07	1.481GluHis: 1.481 ± 0.039	6.28GluIle: 6.28 ± 0.075	7.42GluLys: 7.42 ± 0.092	6.885GluLeu: 6.885 ± 0.083	2.35GluMet: 2.35 ± 0.052	4.107GluAsn: 4.107 ± 0.057	1.786GluPro: 1.786 ± 0.04	3.052GluGln: 3.052 ± 0.059	3.213GluArg: 3.213 ± 0.069	3.513GluSer: 3.513 ± 0.054	3.669GluThr: 3.669 ± 0.062	4.693GluVal: 4.693 ± 0.069	0.812GluTrp: 0.812 ± 0.028	2.452GluTyr: 2.452 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	2.899PheAla: 2.899 ± 0.052	0.373PheCys: 0.373 ± 0.02	2.422PheAsp: 2.422 ± 0.05	2.882PheGlu: 2.882 ± 0.051	2.37PhePhe: 2.37 ± 0.05	3.269PheGly: 3.269 ± 0.066	1.078PheHis: 1.078 ± 0.029	4.315PheIle: 4.315 ± 0.072	2.794PheLys: 2.794 ± 0.059	4.467PheLeu: 4.467 ± 0.074	1.112PheMet: 1.112 ± 0.032	2.105PheAsn: 2.105 ± 0.047	1.718PhePro: 1.718 ± 0.04	1.631PheGln: 1.631 ± 0.036	1.524PheArg: 1.524 ± 0.035	3.463PheSer: 3.463 ± 0.061	2.562PheThr: 2.562 ± 0.042	2.992PheVal: 2.992 ± 0.059	0.471PheTrp: 0.471 ± 0.019	1.823PheTyr: 1.823 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	4.104GlyAla: 4.104 ± 0.081	0.572GlyCys: 0.572 ± 0.023	2.978GlyAsp: 2.978 ± 0.06	4.152GlyGlu: 4.152 ± 0.066	3.295GlyPhe: 3.295 ± 0.051	4.441GlyGly: 4.441 ± 0.087	1.306GlyHis: 1.306 ± 0.034	6.052GlyIle: 6.052 ± 0.092	5.613GlyLys: 5.613 ± 0.085	6.074GlyLeu: 6.074 ± 0.081	1.96GlyMet: 1.96 ± 0.045	2.949GlyAsn: 2.949 ± 0.054	1.628GlyPro: 1.628 ± 0.036	2.153GlyGln: 2.153 ± 0.047	2.403GlyArg: 2.403 ± 0.046	3.641GlySer: 3.641 ± 0.058	3.945GlyThr: 3.945 ± 0.061	4.601GlyVal: 4.601 ± 0.066	0.745GlyTrp: 0.745 ± 0.027	2.782GlyTyr: 2.782 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.201HisAla: 1.201 ± 0.032	0.192HisCys: 0.192 ± 0.013	0.966HisAsp: 0.966 ± 0.032	1.316HisGlu: 1.316 ± 0.038	1.128HisPhe: 1.128 ± 0.033	1.34HisGly: 1.34 ± 0.038	0.685HisHis: 0.685 ± 0.029	1.583HisIle: 1.583 ± 0.032	1.121HisLys: 1.121 ± 0.03	2.207HisLeu: 2.207 ± 0.048	0.472HisMet: 0.472 ± 0.022	0.786HisAsn: 0.786 ± 0.026	1.131HisPro: 1.131 ± 0.035	0.849HisGln: 0.849 ± 0.026	0.869HisArg: 0.869 ± 0.029	1.313HisSer: 1.313 ± 0.036	1.048HisThr: 1.048 ± 0.029	1.421HisVal: 1.421 ± 0.038	0.215HisTrp: 0.215 ± 0.016	0.847HisTyr: 0.847 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.904IleAla: 5.904 ± 0.088	0.707IleCys: 0.707 ± 0.024	4.862IleAsp: 4.862 ± 0.068	6.275IleGlu: 6.275 ± 0.084	3.682IlePhe: 3.682 ± 0.068	6.077IleGly: 6.077 ± 0.102	1.857IleHis: 1.857 ± 0.04	7.277IleIle: 7.277 ± 0.096	5.727IleLys: 5.727 ± 0.075	7.549IleLeu: 7.549 ± 0.104	1.899IleMet: 1.899 ± 0.052	4.204IleAsn: 4.204 ± 0.061	3.635IlePro: 3.635 ± 0.054	3.285IleGln: 3.285 ± 0.064	3.334IleArg: 3.334 ± 0.055	5.594IleSer: 5.594 ± 0.084	4.46IleThr: 4.46 ± 0.07	5.782IleVal: 5.782 ± 0.081	0.664IleTrp: 0.664 ± 0.023	2.698IleTyr: 2.698 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	4.82LysAla: 4.82 ± 0.069	0.385LysCys: 0.385 ± 0.021	4.413LysAsp: 4.413 ± 0.071	7.493LysGlu: 7.493 ± 0.091	2.406LysPhe: 2.406 ± 0.053	5.0LysGly: 5.0 ± 0.073	1.454LysHis: 1.454 ± 0.036	5.896LysIle: 5.896 ± 0.074	7.609LysLys: 7.609 ± 0.102	6.698LysLeu: 6.698 ± 0.078	2.476LysMet: 2.476 ± 0.045	4.543LysAsn: 4.543 ± 0.073	2.39LysPro: 2.39 ± 0.056	3.445LysGln: 3.445 ± 0.06	3.499LysArg: 3.499 ± 0.057	4.172LysSer: 4.172 ± 0.074	4.068LysThr: 4.068 ± 0.061	5.23LysVal: 5.23 ± 0.08	0.901LysTrp: 0.901 ± 0.027	2.78LysTyr: 2.78 ± 0.054	0.0LysXaa: 0.0 ± 0.0
Leu	6.317LeuAla: 6.317 ± 0.094	0.691LeuCys: 0.691 ± 0.028	4.686LeuAsp: 4.686 ± 0.074	6.338LeuGlu: 6.338 ± 0.085	4.801LeuPhe: 4.801 ± 0.073	6.012LeuGly: 6.012 ± 0.089	1.938LeuHis: 1.938 ± 0.04	7.568LeuIle: 7.568 ± 0.108	7.472LeuLys: 7.472 ± 0.08	9.644LeuLeu: 9.644 ± 0.125	2.406LeuMet: 2.406 ± 0.05	4.763LeuAsn: 4.763 ± 0.075	3.667LeuPro: 3.667 ± 0.051	3.62LeuGln: 3.62 ± 0.064	3.37LeuArg: 3.37 ± 0.062	6.895LeuSer: 6.895 ± 0.093	5.408LeuThr: 5.408 ± 0.072	5.727LeuVal: 5.727 ± 0.072	0.786LeuTrp: 0.786 ± 0.029	3.185LeuTyr: 3.185 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	1.805MetAla: 1.805 ± 0.041	0.143MetCys: 0.143 ± 0.012	1.446MetAsp: 1.446 ± 0.034	1.947MetGlu: 1.947 ± 0.044	1.048MetPhe: 1.048 ± 0.034	1.718MetGly: 1.718 ± 0.04	0.417MetHis: 0.417 ± 0.019	2.196MetIle: 2.196 ± 0.051	2.748MetLys: 2.748 ± 0.051	2.375MetLeu: 2.375 ± 0.051	0.804MetMet: 0.804 ± 0.028	1.707MetAsn: 1.707 ± 0.04	0.91MetPro: 0.91 ± 0.033	0.828MetGln: 0.828 ± 0.03	0.984MetArg: 0.984 ± 0.026	1.654MetSer: 1.654 ± 0.037	1.493MetThr: 1.493 ± 0.036	1.623MetVal: 1.623 ± 0.039	0.18MetTrp: 0.18 ± 0.013	0.777MetTyr: 0.777 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.768AsnAla: 2.768 ± 0.047	0.342AsnCys: 0.342 ± 0.017	2.696AsnAsp: 2.696 ± 0.047	4.126AsnGlu: 4.126 ± 0.067	1.903AsnPhe: 1.903 ± 0.044	3.635AsnGly: 3.635 ± 0.068	1.189AsnHis: 1.189 ± 0.037	4.127AsnIle: 4.127 ± 0.067	3.946AsnLys: 3.946 ± 0.07	4.15AsnLeu: 4.15 ± 0.068	1.298AsnMet: 1.298 ± 0.033	2.606AsnAsn: 2.606 ± 0.061	2.282AsnPro: 2.282 ± 0.05	2.226AsnGln: 2.226 ± 0.057	2.225AsnArg: 2.225 ± 0.046	2.649AsnSer: 2.649 ± 0.051	2.349AsnThr: 2.349 ± 0.039	3.199AsnVal: 3.199 ± 0.054	0.58AsnTrp: 0.58 ± 0.02	1.765AsnTyr: 1.765 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	1.882ProAla: 1.882 ± 0.045	0.231ProCys: 0.231 ± 0.015	1.836ProAsp: 1.836 ± 0.046	2.674ProGlu: 2.674 ± 0.056	1.989ProPhe: 1.989 ± 0.04	2.104ProGly: 2.104 ± 0.042	0.753ProHis: 0.753 ± 0.026	3.163ProIle: 3.163 ± 0.055	2.599ProLys: 2.599 ± 0.048	3.353ProLeu: 3.353 ± 0.057	0.801ProMet: 0.801 ± 0.029	1.956ProAsn: 1.956 ± 0.047	0.981ProPro: 0.981 ± 0.036	1.092ProGln: 1.092 ± 0.037	1.021ProArg: 1.021 ± 0.031	2.234ProSer: 2.234 ± 0.044	2.039ProThr: 2.039 ± 0.046	2.577ProVal: 2.577 ± 0.048	0.348ProTrp: 0.348 ± 0.019	1.466ProTyr: 1.466 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.35GlnAla: 2.35 ± 0.049	0.215GlnCys: 0.215 ± 0.015	1.584GlnAsp: 1.584 ± 0.034	2.569GlnGlu: 2.569 ± 0.049	1.785GlnPhe: 1.785 ± 0.04	2.04GlnGly: 2.04 ± 0.043	0.806GlnHis: 0.806 ± 0.026	2.932GlnIle: 2.932 ± 0.049	3.15GlnLys: 3.15 ± 0.056	4.129GlnLeu: 4.129 ± 0.062	1.111GlnMet: 1.111 ± 0.032	1.833GlnAsn: 1.833 ± 0.042	1.131GlnPro: 1.131 ± 0.033	1.784GlnGln: 1.784 ± 0.05	1.421GlnArg: 1.421 ± 0.037	2.207GlnSer: 2.207 ± 0.048	2.032GlnThr: 2.032 ± 0.041	2.211GlnVal: 2.211 ± 0.038	0.414GlnTrp: 0.414 ± 0.02	1.457GlnTyr: 1.457 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.182ArgAla: 2.182 ± 0.044	0.244ArgCys: 0.244 ± 0.014	1.888ArgAsp: 1.888 ± 0.047	2.943ArgGlu: 2.943 ± 0.058	1.902ArgPhe: 1.902 ± 0.044	2.176ArgGly: 2.176 ± 0.039	0.714ArgHis: 0.714 ± 0.026	3.128ArgIle: 3.128 ± 0.057	3.609ArgLys: 3.609 ± 0.061	3.852ArgLeu: 3.852 ± 0.06	1.235ArgMet: 1.235 ± 0.031	2.034ArgAsn: 2.034 ± 0.051	1.233ArgPro: 1.233 ± 0.035	1.412ArgGln: 1.412 ± 0.038	1.68ArgArg: 1.68 ± 0.044	2.148ArgSer: 2.148 ± 0.048	1.985ArgThr: 1.985 ± 0.045	2.39ArgVal: 2.39 ± 0.049	0.41ArgTrp: 0.41 ± 0.018	1.502ArgTyr: 1.502 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	3.389SerAla: 3.389 ± 0.062	0.429SerCys: 0.429 ± 0.019	2.821SerAsp: 2.821 ± 0.059	4.001SerGlu: 4.001 ± 0.063	3.351SerPhe: 3.351 ± 0.06	4.268SerGly: 4.268 ± 0.068	1.236SerHis: 1.236 ± 0.033	5.551SerIle: 5.551 ± 0.073	4.667SerLys: 4.667 ± 0.073	6.146SerLeu: 6.146 ± 0.08	1.634SerMet: 1.634 ± 0.038	2.866SerAsn: 2.866 ± 0.057	2.125SerPro: 2.125 ± 0.046	1.96SerGln: 1.96 ± 0.042	2.211SerArg: 2.211 ± 0.049	4.063SerSer: 4.063 ± 0.071	3.22SerThr: 3.22 ± 0.059	3.948SerVal: 3.948 ± 0.058	0.612SerTrp: 0.612 ± 0.022	2.305SerTyr: 2.305 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	3.514ThrAla: 3.514 ± 0.061	0.367ThrCys: 0.367 ± 0.02	2.524ThrAsp: 2.524 ± 0.044	3.245ThrGlu: 3.245 ± 0.051	2.626ThrPhe: 2.626 ± 0.05	3.852ThrGly: 3.852 ± 0.062	1.021ThrHis: 1.021 ± 0.027	5.059ThrIle: 5.059 ± 0.075	3.867ThrLys: 3.867 ± 0.073	5.018ThrLeu: 5.018 ± 0.072	1.259ThrMet: 1.259 ± 0.034	2.821ThrAsn: 2.821 ± 0.053	2.215ThrPro: 2.215 ± 0.045	1.428ThrGln: 1.428 ± 0.038	1.82ThrArg: 1.82 ± 0.041	3.302ThrSer: 3.302 ± 0.059	2.943ThrThr: 2.943 ± 0.056	3.846ThrVal: 3.846 ± 0.063	0.521ThrTrp: 0.521 ± 0.021	1.9ThrTyr: 1.9 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	4.319ValAla: 4.319 ± 0.072	0.56ValCys: 0.56 ± 0.024	3.584ValAsp: 3.584 ± 0.064	4.535ValGlu: 4.535 ± 0.067	3.01ValPhe: 3.01 ± 0.066	4.308ValGly: 4.308 ± 0.071	1.254ValHis: 1.254 ± 0.034	5.679ValIle: 5.679 ± 0.066	5.067ValLys: 5.067 ± 0.076	6.26ValLeu: 6.26 ± 0.082	1.66ValMet: 1.66 ± 0.039	3.305ValAsn: 3.305 ± 0.064	2.453ValPro: 2.453 ± 0.048	2.218ValGln: 2.218 ± 0.05	2.451ValArg: 2.451 ± 0.05	4.21ValSer: 4.21 ± 0.067	3.653ValThr: 3.653 ± 0.061	4.517ValVal: 4.517 ± 0.066	0.566ValTrp: 0.566 ± 0.025	2.174ValTyr: 2.174 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.549TrpAla: 0.549 ± 0.024	0.09TrpCys: 0.09 ± 0.008	0.547TrpAsp: 0.547 ± 0.021	0.641TrpGlu: 0.641 ± 0.026	0.507TrpPhe: 0.507 ± 0.024	0.638TrpGly: 0.638 ± 0.026	0.234TrpHis: 0.234 ± 0.015	0.891TrpIle: 0.891 ± 0.029	0.818TrpLys: 0.818 ± 0.03	1.041TrpLeu: 1.041 ± 0.033	0.309TrpMet: 0.309 ± 0.017	0.591TrpAsn: 0.591 ± 0.023	0.239TrpPro: 0.239 ± 0.015	0.313TrpGln: 0.313 ± 0.016	0.403TrpArg: 0.403 ± 0.019	0.597TrpSer: 0.597 ± 0.025	0.51TrpThr: 0.51 ± 0.026	0.632TrpVal: 0.632 ± 0.028	0.139TrpTrp: 0.139 ± 0.013	0.355TrpTyr: 0.355 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.909TyrAla: 1.909 ± 0.038	0.36TyrCys: 0.36 ± 0.017	1.979TyrAsp: 1.979 ± 0.045	2.632TyrGlu: 2.632 ± 0.046	1.958TyrPhe: 1.958 ± 0.046	2.421TyrGly: 2.421 ± 0.051	0.869TyrHis: 0.869 ± 0.03	2.799TyrIle: 2.799 ± 0.059	2.509TyrLys: 2.509 ± 0.051	3.71TyrLeu: 3.71 ± 0.064	0.862TyrMet: 0.862 ± 0.027	1.646TyrAsn: 1.646 ± 0.039	1.405TyrPro: 1.405 ± 0.042	1.605TyrGln: 1.605 ± 0.042	1.608TyrArg: 1.608 ± 0.037	2.27TyrSer: 2.27 ± 0.042	1.839TyrThr: 1.839 ± 0.039	2.126TyrVal: 2.126 ± 0.038	0.404TyrTrp: 0.404 ± 0.019	1.519TyrTyr: 1.519 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4209 proteins (1127785 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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