Kwoniella heveanensis BCC8398

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Tremellales; Cryptococcaceae; Kwoniella; Kwoniella heveanensis

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7924 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B9GJ44|A0A1B9GJ44_9TREE Cysteine proteinase 1 mitochondrial OS=Kwoniella heveanensis BCC8398 OX=1296120 GN=I316_07361 PE=3 SV=1
MM1 pKa = 7.81LYY3 pKa = 10.55NRR5 pKa = 11.84LFSLLPLLPLVFSAPLPLPLLGLDD29 pKa = 4.73DD30 pKa = 5.08LAATDD35 pKa = 5.65GILDD39 pKa = 4.26PLLNTQDD46 pKa = 3.77FAVPPPSSSDD56 pKa = 3.27PSGPALGPGGLLSGLLGNDD75 pKa = 3.21NDD77 pKa = 3.9QSEE80 pKa = 4.61GLLGSLVDD88 pKa = 3.88TVTDD92 pKa = 3.06ITGPLNISLGLNTSILGTDD111 pKa = 3.87LDD113 pKa = 4.56LGLNIQLGDD122 pKa = 4.27DD123 pKa = 3.9EE124 pKa = 5.3EE125 pKa = 5.28LICGSVNGVWDD136 pKa = 3.68GTSYY140 pKa = 11.49DD141 pKa = 4.11LRR143 pKa = 11.84CVCWSVEE150 pKa = 3.8KK151 pKa = 10.98GIVIDD156 pKa = 4.06AQVGADD162 pKa = 2.97IGLGEE167 pKa = 4.87RR168 pKa = 11.84EE169 pKa = 4.17GLDD172 pKa = 3.0AFLRR176 pKa = 11.84AQIQFGGHH184 pKa = 5.46KK185 pKa = 9.67FAYY188 pKa = 8.51PAYY191 pKa = 10.21AEE193 pKa = 4.29PTCDD197 pKa = 3.05GSGGFNCPGGRR208 pKa = 11.84QANGKK213 pKa = 9.81CSAFLAAKK221 pKa = 8.72PRR223 pKa = 11.84PRR225 pKa = 11.84PIPAADD231 pKa = 3.9AGSPAPTPTPAPAAAAIPPATDD253 pKa = 3.46LQINSVPPTTISASATTTTAAAAATVNSPDD283 pKa = 3.57APQAQAQDD291 pKa = 3.62EE292 pKa = 4.83TFGIQAVPAVAAAVTQFGTTTTTSMSTEE320 pKa = 4.3TIVLPATVFVEE331 pKa = 4.73MITTTEE337 pKa = 3.61MSTIYY342 pKa = 9.97ATVTQTEE349 pKa = 4.54VSTSISTATVTSTQTSTQTQWTTATSTVSTCSTTAEE385 pKa = 4.02DD386 pKa = 3.51EE387 pKa = 4.41QINVDD392 pKa = 4.19SVAQPTTSVTGYY404 pKa = 8.5TPASSTLVTSTSSSSATPAPEE425 pKa = 3.73VTIAATPAPATGPATSGSTGGVPAPDD451 pKa = 3.9PTSTPTPAVDD461 pKa = 4.92NGDD464 pKa = 3.94GDD466 pKa = 5.55DD467 pKa = 5.46DD468 pKa = 4.36DD469 pKa = 4.83TFSGPRR475 pKa = 11.84VIDD478 pKa = 3.45LAKK481 pKa = 9.39PQSHH485 pKa = 6.78GSTTCPEE492 pKa = 3.89GQAWGSTMCCRR503 pKa = 11.84TDD505 pKa = 3.32QVEE508 pKa = 3.97VDD510 pKa = 4.63GEE512 pKa = 4.71CKK514 pKa = 10.53CHH516 pKa = 7.38DD517 pKa = 3.8GFEE520 pKa = 4.87NIVDD524 pKa = 3.62VCLRR528 pKa = 11.84LCIGDD533 pKa = 3.86RR534 pKa = 11.84LPSGEE539 pKa = 4.45CSILGLGLDD548 pKa = 4.24LGVGLGGLEE557 pKa = 4.02LL558 pKa = 4.9

Molecular weight: 56.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B9GKX1|A0A1B9GKX1_9TREE BRCT domain-containing protein OS=Kwoniella heveanensis BCC8398 OX=1296120 GN=I316_06790 PE=4 SV=1
MM1 pKa = 7.56PASPQICEE9 pKa = 3.94NCGLPRR15 pKa = 11.84AFPPSPRR22 pKa = 11.84DD23 pKa = 3.28LPTMFEE29 pKa = 4.22EE30 pKa = 4.53DD31 pKa = 3.6FEE33 pKa = 5.16RR34 pKa = 11.84CFICEE39 pKa = 4.09KK40 pKa = 10.24ACKK43 pKa = 9.98GLYY46 pKa = 9.87CSSEE50 pKa = 3.78CRR52 pKa = 11.84LRR54 pKa = 11.84DD55 pKa = 3.24QGTPSPAVPAAKK67 pKa = 9.45MGPVKK72 pKa = 9.79ITSQLPASLSPLVRR86 pKa = 11.84PAHH89 pKa = 5.9HH90 pKa = 7.59PGRR93 pKa = 11.84SPRR96 pKa = 11.84VFAQNRR102 pKa = 11.84ASSSISSGSSSVSSSPLQSPQTNPSEE128 pKa = 4.09ADD130 pKa = 3.44SPKK133 pKa = 10.49KK134 pKa = 9.97DD135 pKa = 3.64TFDD138 pKa = 4.63LPPPAYY144 pKa = 8.39PFKK147 pKa = 11.07VGGIPTSVPMKK158 pKa = 10.23IPALIPRR165 pKa = 11.84AAVAQQPHH173 pKa = 5.98GQTPGSQGSTIYY185 pKa = 10.23PAGTSIDD192 pKa = 3.44TLRR195 pKa = 11.84FGRR198 pKa = 11.84KK199 pKa = 8.36PSVVNNVISPNALIPRR215 pKa = 11.84CACGLPANHH224 pKa = 7.6KK225 pKa = 10.21GRR227 pKa = 11.84ANSKK231 pKa = 10.36DD232 pKa = 3.45RR233 pKa = 11.84ADD235 pKa = 3.81LPEE238 pKa = 5.51SGFSRR243 pKa = 11.84LSLGPSVVNPPHH255 pKa = 6.9ASEE258 pKa = 4.41EE259 pKa = 3.97PGPRR263 pKa = 11.84SFRR266 pKa = 11.84IVSDD270 pKa = 3.41SAIPPFPGRR279 pKa = 11.84PVQPSVPTPSRR290 pKa = 11.84ASIPLSIPQSPQVNPTASLLSRR312 pKa = 11.84SRR314 pKa = 11.84SDD316 pKa = 4.84PIPSSPQVQRR326 pKa = 11.84RR327 pKa = 11.84AKK329 pKa = 9.47PIPAAPAPAPIIATNVITPSHH350 pKa = 7.11RR351 pKa = 11.84EE352 pKa = 3.71TFAVPMSPIVPAQASACRR370 pKa = 11.84PSRR373 pKa = 11.84HH374 pKa = 5.64TPNALEE380 pKa = 4.14VSMASPRR387 pKa = 11.84RR388 pKa = 11.84GRR390 pKa = 11.84SRR392 pKa = 11.84EE393 pKa = 3.94RR394 pKa = 11.84QEE396 pKa = 3.86HH397 pKa = 6.45HH398 pKa = 6.42VPQMTGNALGGPADD412 pKa = 4.23RR413 pKa = 11.84EE414 pKa = 4.23QAPSRR419 pKa = 11.84SRR421 pKa = 11.84NRR423 pKa = 11.84RR424 pKa = 11.84DD425 pKa = 2.76SRR427 pKa = 11.84RR428 pKa = 11.84RR429 pKa = 11.84SDD431 pKa = 2.95SRR433 pKa = 11.84EE434 pKa = 3.65RR435 pKa = 11.84EE436 pKa = 3.88RR437 pKa = 11.84EE438 pKa = 3.78RR439 pKa = 11.84EE440 pKa = 3.92RR441 pKa = 11.84GRR443 pKa = 11.84GRR445 pKa = 11.84AGVVVGDD452 pKa = 4.15RR453 pKa = 11.84EE454 pKa = 4.33RR455 pKa = 11.84EE456 pKa = 3.91HH457 pKa = 7.56SGQRR461 pKa = 11.84SPRR464 pKa = 11.84SPLATTNGTVDD475 pKa = 3.99PPQILPSWSRR485 pKa = 11.84RR486 pKa = 11.84ASEE489 pKa = 3.81ATADD493 pKa = 3.62MRR495 pKa = 11.84KK496 pKa = 9.48LVGDD500 pKa = 4.01GVVTPVMRR508 pKa = 11.84RR509 pKa = 11.84TSSGDD514 pKa = 2.99KK515 pKa = 10.22KK516 pKa = 10.95SPVYY520 pKa = 10.45EE521 pKa = 3.93RR522 pKa = 11.84DD523 pKa = 3.12EE524 pKa = 4.25EE525 pKa = 4.0EE526 pKa = 4.28RR527 pKa = 11.84EE528 pKa = 4.08RR529 pKa = 11.84KK530 pKa = 9.6RR531 pKa = 11.84EE532 pKa = 3.85EE533 pKa = 3.68LKK535 pKa = 10.79RR536 pKa = 11.84ASKK539 pKa = 10.63QLGQVFGVAAGG550 pKa = 3.38

Molecular weight: 59.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7924

0

7924

4255186

44

5007

537.0

58.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.998 ± 0.028

0.937 ± 0.009

5.611 ± 0.019

6.203 ± 0.026

3.188 ± 0.016

7.705 ± 0.026

2.369 ± 0.013

4.544 ± 0.016

4.738 ± 0.022

8.54 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.019 ± 0.011

3.325 ± 0.014

6.75 ± 0.033

3.849 ± 0.022

6.015 ± 0.023

9.498 ± 0.036

6.046 ± 0.019

5.907 ± 0.018

1.316 ± 0.01

2.442 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski