Enterobacter phage EspM4VN

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Aglimvirinae; Agtrevirus; unclassified Agtrevirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 219 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z6C8P7|A0A2Z6C8P7_9CAUD Putative loader of gp41 DNA helicase gp59 OS=Enterobacter phage EspM4VN OX=2137745 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.23LNKK5 pKa = 9.49ILLVCALAFSTTACSTLLDD24 pKa = 3.75VASTVDD30 pKa = 3.92LDD32 pKa = 4.67APTFTNEE39 pKa = 3.47QATSKK44 pKa = 10.47MEE46 pKa = 4.04DD47 pKa = 3.45TIKK50 pKa = 11.01AHH52 pKa = 6.91AALEE56 pKa = 4.24NTTPGPLQTVCNYY69 pKa = 10.58DD70 pKa = 3.97DD71 pKa = 5.54SIQEE75 pKa = 4.24DD76 pKa = 3.93EE77 pKa = 4.95TYY79 pKa = 10.8HH80 pKa = 5.42CTTYY84 pKa = 10.89VKK86 pKa = 10.16QASVVLYY93 pKa = 10.43ADD95 pKa = 3.89CTEE98 pKa = 4.24EE99 pKa = 3.99QCVATGYY106 pKa = 11.29DD107 pKa = 3.23KK108 pKa = 11.7VEE110 pKa = 3.94EE111 pKa = 4.53DD112 pKa = 3.61KK113 pKa = 11.58

Molecular weight: 12.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P2WVE0|A0A4P2WVE0_9CAUD Uncharacterized protein OS=Enterobacter phage EspM4VN OX=2137745 PE=4 SV=1
MM1 pKa = 7.27TAFNQYY7 pKa = 8.32TKK9 pKa = 10.95ALDD12 pKa = 3.72GVGPVSIDD20 pKa = 3.61RR21 pKa = 11.84IKK23 pKa = 11.12SLAAEE28 pKa = 4.42FFPYY32 pKa = 9.19DD33 pKa = 3.64TANPGQMEE41 pKa = 5.15CIVEE45 pKa = 4.18AVDD48 pKa = 3.99ALLNKK53 pKa = 9.43KK54 pKa = 9.0VKK56 pKa = 10.21HH57 pKa = 5.9VIIEE61 pKa = 4.21APTGVGKK68 pKa = 10.7SSSALPFIMCFAIWFLSAIRR88 pKa = 11.84MASSARR94 pKa = 11.84LSRR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84PKK101 pKa = 9.85VCRR104 pKa = 11.84ISMRR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84KK111 pKa = 9.05PWRR114 pKa = 3.62

Molecular weight: 12.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

219

0

219

48425

52

1586

221.1

24.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.319 ± 0.174

1.171 ± 0.07

6.546 ± 0.119

6.447 ± 0.176

4.337 ± 0.104

6.798 ± 0.198

1.89 ± 0.106

5.885 ± 0.122

6.179 ± 0.179

8.095 ± 0.121

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.91 ± 0.107

4.927 ± 0.13

3.965 ± 0.113

3.657 ± 0.12

5.068 ± 0.131

5.929 ± 0.15

6.197 ± 0.218

7.482 ± 0.16

1.429 ± 0.077

3.771 ± 0.109
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski