Gordonia phage DelRio
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345KW64|A0A345KW64_9CAUD Uncharacterized protein OS=Gordonia phage DelRio OX=2250288 GN=46 PE=4 SV=1
MM1 pKa = 7.2 TPDD4 pKa = 3.27 VSAVTLTTTYY14 pKa = 8.2 TQPADD19 pKa = 3.58 GWPLAALDD27 pKa = 3.8 AFGIMFAPDD36 pKa = 4.54 TITITASADD45 pKa = 3.31 TTGTVEE51 pKa = 4.05 VLPVVSVAGHH61 pKa = 6.6 IGDD64 pKa = 3.83 QAVTTSANVGDD75 pKa = 4.24 LPAYY79 pKa = 9.39 VLEE82 pKa = 4.52 LVEE85 pKa = 4.18 HH86 pKa = 6.67 AKK88 pKa = 10.4 LAAAHH93 pKa = 6.45 ALAVAA98 pKa = 4.67
Molecular weight: 9.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.948
IPC_protein 3.846
Toseland 3.643
ProMoST 4.05
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.795
Nozaki 3.999
DTASelect 4.228
Thurlkill 3.732
EMBOSS 3.846
Sillero 3.986
Patrickios 1.926
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.868
Protein with the highest isoelectric point:
>tr|A0A345KW34|A0A345KW34_9CAUD Uncharacterized protein OS=Gordonia phage DelRio OX=2250288 GN=16 PE=4 SV=1
MM1 pKa = 7.25 PRR3 pKa = 11.84 VRR5 pKa = 11.84 AWLTDD10 pKa = 3.4 NLVEE14 pKa = 4.24 AEE16 pKa = 4.2 VRR18 pKa = 11.84 ADD20 pKa = 4.15 VPANWTLAAATRR32 pKa = 11.84 PLVVVADD39 pKa = 4.35 DD40 pKa = 4.71 GGPVDD45 pKa = 3.35 WPVRR49 pKa = 11.84 SEE51 pKa = 3.66 HH52 pKa = 6.35 TIRR55 pKa = 11.84 LVTRR59 pKa = 11.84 AKK61 pKa = 10.57 SRR63 pKa = 11.84 TEE65 pKa = 3.07 ARR67 pKa = 11.84 TIVRR71 pKa = 11.84 LAAGRR76 pKa = 11.84 LHH78 pKa = 5.57 TAKK81 pKa = 10.7 LSGIVVRR88 pKa = 11.84 RR89 pKa = 11.84 AGGSVVEE96 pKa = 4.63 ATDD99 pKa = 3.58 KK100 pKa = 11.12 ATGAYY105 pKa = 8.52 LASVLVPIQARR116 pKa = 11.84 TVEE119 pKa = 4.18 LL120 pKa = 3.82
Molecular weight: 12.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.472
IPC_protein 10.599
Toseland 10.847
ProMoST 10.877
Dawson 10.891
Bjellqvist 10.701
Wikipedia 11.199
Rodwell 10.862
Grimsley 10.935
Solomon 11.169
Lehninger 11.111
Nozaki 10.818
DTASelect 10.701
Thurlkill 10.833
EMBOSS 11.286
Sillero 10.847
Patrickios 10.657
IPC_peptide 11.184
IPC2_peptide 9.794
IPC2.peptide.svr19 8.954
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
75
0
75
16089
37
1751
214.5
23.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.052 ± 0.406
0.876 ± 0.155
7.21 ± 0.221
5.258 ± 0.26
2.523 ± 0.144
9.404 ± 0.434
2.026 ± 0.198
4.003 ± 0.211
2.946 ± 0.366
7.993 ± 0.233
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.834 ± 0.108
2.704 ± 0.143
5.718 ± 0.335
3.089 ± 0.206
7.378 ± 0.423
5.159 ± 0.266
6.912 ± 0.268
7.993 ± 0.235
2.02 ± 0.112
1.902 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here