Gigaspora margarita mitovirus 2
Average proteome isoelectric point is 8.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L0VZH6|A0A2L0VZH6_9VIRU RNA-dependent RNA polymerase OS=Gigaspora margarita mitovirus 2 OX=2082666 PE=4 SV=1
MM1 pKa = 7.61 TSFKK5 pKa = 10.59 YY6 pKa = 10.12 IKK8 pKa = 10.3 CSAPQTGWTFLSFRR22 pKa = 11.84 AFNTGSACLQVLLDD36 pKa = 5.06 HH37 pKa = 6.5 PLIWSVDD44 pKa = 3.26 WFKK47 pKa = 11.75 VKK49 pKa = 10.68 AGFYY53 pKa = 10.27 CIVDD57 pKa = 3.4 PTNPRR62 pKa = 11.84 EE63 pKa = 4.02 LIYY66 pKa = 10.87 LGEE69 pKa = 4.04 KK70 pKa = 10.32 DD71 pKa = 3.84 FRR73 pKa = 11.84 DD74 pKa = 4.97 FIKK77 pKa = 11.09 VMLSNRR83 pKa = 11.84 EE84 pKa = 3.84 PMVVLARR91 pKa = 11.84 AGEE94 pKa = 4.03 NQPKK98 pKa = 10.16 DD99 pKa = 3.98 SEE101 pKa = 4.29 ILTPKK106 pKa = 9.73 PKK108 pKa = 10.24 SPHH111 pKa = 4.81 STVFFRR117 pKa = 11.84 SFRR120 pKa = 11.84 VYY122 pKa = 11.1 SKK124 pKa = 10.37 FLRR127 pKa = 11.84 ALWSEE132 pKa = 3.78 MWEE135 pKa = 4.35 GKK137 pKa = 10.27 LVVPLEE143 pKa = 4.33 RR144 pKa = 11.84 NLKK147 pKa = 10.37 FIICNWGKK155 pKa = 7.75 TLLHH159 pKa = 6.36 WLGTSSSSRR168 pKa = 11.84 AQSQGLIQVSKK179 pKa = 11.04 YY180 pKa = 10.85 LMVVLKK186 pKa = 10.8 HH187 pKa = 6.35 RR188 pKa = 11.84 GATGLTIFLKK198 pKa = 10.94 VSFIALNKK206 pKa = 9.35 YY207 pKa = 9.82 LAGEE211 pKa = 4.14 PLKK214 pKa = 10.62 STFPLKK220 pKa = 10.58 CGMRR224 pKa = 11.84 LQGGLPSWLPAPVRR238 pKa = 11.84 LAIRR242 pKa = 11.84 QKK244 pKa = 8.72 SYY246 pKa = 10.97 KK247 pKa = 10.17 VIRR250 pKa = 11.84 LWSSLLYY257 pKa = 9.71 TYY259 pKa = 10.95 KK260 pKa = 10.85 GMYY263 pKa = 9.64 AKK265 pKa = 10.02 GVPSIASVLEE275 pKa = 4.17 PEE277 pKa = 4.87 WYY279 pKa = 9.31 TSSTRR284 pKa = 11.84 ATWLWGFQDD293 pKa = 4.04 FLANVYY299 pKa = 9.47 YY300 pKa = 10.57 RR301 pKa = 11.84 QLNWVGLDD309 pKa = 3.47 IPQSDD314 pKa = 4.2 QLPLLRR320 pKa = 11.84 TSAGPSGGTALAGVLRR336 pKa = 11.84 DD337 pKa = 3.29 LWVWRR342 pKa = 11.84 VFRR345 pKa = 11.84 HH346 pKa = 5.42 NRR348 pKa = 11.84 LHH350 pKa = 6.71 YY351 pKa = 10.66 SLEE354 pKa = 4.02 QILAYY359 pKa = 9.19 TGNGTALGFLRR370 pKa = 11.84 HH371 pKa = 5.73 GLAGAKK377 pKa = 9.43 PSQVFPLKK385 pKa = 9.18 VHH387 pKa = 6.72 KK388 pKa = 10.45 KK389 pKa = 9.27 GSCQEE394 pKa = 4.31 GYY396 pKa = 10.77 DD397 pKa = 3.09 IALYY401 pKa = 10.5 DD402 pKa = 3.56 GRR404 pKa = 11.84 KK405 pKa = 8.67 PLALARR411 pKa = 11.84 LHH413 pKa = 6.92 LIYY416 pKa = 10.38 EE417 pKa = 4.36 AAGKK421 pKa = 10.1 VRR423 pKa = 11.84 VIAILDD429 pKa = 3.85 YY430 pKa = 7.63 MTQWAFAPVHH440 pKa = 6.42 DD441 pKa = 4.27 FLMRR445 pKa = 11.84 LLATVDD451 pKa = 3.23 TDD453 pKa = 3.62 GTSDD457 pKa = 4.42 QNIGLQNFILRR468 pKa = 11.84 IGSRR472 pKa = 11.84 KK473 pKa = 9.86 GIYY476 pKa = 9.86 SSLDD480 pKa = 2.78 ISAATDD486 pKa = 3.86 TIPWQLYY493 pKa = 7.45 EE494 pKa = 4.33 VILGVMFDD502 pKa = 3.54 ARR504 pKa = 11.84 FARR507 pKa = 11.84 HH508 pKa = 5.05 YY509 pKa = 9.97 MRR511 pKa = 11.84 LLRR514 pKa = 11.84 EE515 pKa = 4.12 RR516 pKa = 11.84 GFLSPRR522 pKa = 11.84 EE523 pKa = 4.0 LPFEE527 pKa = 3.99 FVKK530 pKa = 10.89 YY531 pKa = 10.77 GCGQPMGALSSFPLLGLVHH550 pKa = 6.42 HH551 pKa = 7.27 AIVQYY556 pKa = 10.83 AAYY559 pKa = 8.25 EE560 pKa = 4.15 ANSFPFYY567 pKa = 10.38 DD568 pKa = 3.75 YY569 pKa = 11.22 CIVGDD574 pKa = 3.98 DD575 pKa = 3.63 LVLFEE580 pKa = 5.0 EE581 pKa = 5.01 DD582 pKa = 3.11 KK583 pKa = 11.18 AVPVATKK590 pKa = 9.62 YY591 pKa = 11.28 LSMCRR596 pKa = 11.84 YY597 pKa = 9.63 LGIRR601 pKa = 11.84 INKK604 pKa = 8.71 SKK606 pKa = 9.54 TYY608 pKa = 9.34 QSEE611 pKa = 4.43 VFFNFISRR619 pKa = 11.84 SFLNGIEE626 pKa = 4.01 VSPASMKK633 pKa = 10.69 SEE635 pKa = 3.97 LSVHH639 pKa = 5.4 TLGQRR644 pKa = 11.84 VEE646 pKa = 4.08 NTLRR650 pKa = 11.84 NCSRR654 pKa = 11.84 WSTGDD659 pKa = 2.97 SRR661 pKa = 11.84 SVMTRR666 pKa = 11.84 IIRR669 pKa = 11.84 FASDD673 pKa = 2.38 HH674 pKa = 5.87 WVWGTLSVSLSQGFLTSYY692 pKa = 10.18 VATVLASLLSPAGRR706 pKa = 11.84 FSSIFGVKK714 pKa = 9.77 EE715 pKa = 3.7 EE716 pKa = 4.08 GWIYY720 pKa = 9.99 WVAALRR726 pKa = 11.84 GSSIILSHH734 pKa = 7.21 DD735 pKa = 3.05 ASALTTHH742 pKa = 6.0 YY743 pKa = 10.91 LKK745 pKa = 11.08 NNYY748 pKa = 9.77 SKK750 pKa = 11.23 VLILIRR756 pKa = 11.84 TTCLVLRR763 pKa = 11.84 SEE765 pKa = 4.59 LEE767 pKa = 3.96 SLLVSGTKK775 pKa = 9.8 IADD778 pKa = 4.6 LYY780 pKa = 9.09 PSWMVKK786 pKa = 7.32 QPRR789 pKa = 11.84 GIRR792 pKa = 11.84 LLYY795 pKa = 10.17 QLFRR799 pKa = 11.84 EE800 pKa = 4.11 QRR802 pKa = 11.84 VNYY805 pKa = 10.15 AYY807 pKa = 10.81 FNFSSRR813 pKa = 11.84 EE814 pKa = 3.76 VRR816 pKa = 11.84 PLLGYY821 pKa = 9.43 PIAEE825 pKa = 4.19 SSSLLDD831 pKa = 3.69 EE832 pKa = 5.16 PDD834 pKa = 3.46 YY835 pKa = 11.33 EE836 pKa = 5.92 IITGMSHH843 pKa = 6.5 EE844 pKa = 4.62 EE845 pKa = 4.14 LIEE848 pKa = 4.27 SYY850 pKa = 10.96 IMEE853 pKa = 4.43 LLIYY857 pKa = 10.73 SMGYY861 pKa = 9.02 LVPRR865 pKa = 11.84 KK866 pKa = 8.86 FVPYY870 pKa = 9.97 LQEE873 pKa = 5.2 FYY875 pKa = 10.29 EE876 pKa = 4.22 AWSFIFSDD884 pKa = 4.03 NPDD887 pKa = 3.87 PEE889 pKa = 4.42 QLGQYY894 pKa = 7.54 LTQILEE900 pKa = 3.99 AVLAFKK906 pKa = 10.47 PDD908 pKa = 3.28 LDD910 pKa = 3.98 YY911 pKa = 11.63 RR912 pKa = 11.84 DD913 pKa = 3.59 VDD915 pKa = 3.83 VLSKK919 pKa = 8.86 VTRR922 pKa = 11.84 SEE924 pKa = 4.21 FGHH927 pKa = 5.58 TPRR930 pKa = 11.84 SSVINSNKK938 pKa = 9.3 FVYY941 pKa = 10.23 AKK943 pKa = 10.46 SGFANEE949 pKa = 3.92 LLQRR953 pKa = 11.84 LTIAWRR959 pKa = 11.84 MILSMEE965 pKa = 4.05 NLGINPEE972 pKa = 4.15 EE973 pKa = 4.21 TLKK976 pKa = 11.24 LPLPTSSFKK985 pKa = 10.74 EE986 pKa = 3.99
Molecular weight: 112.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.707
IPC2_protein 8.741
IPC_protein 8.639
Toseland 9.063
ProMoST 9.033
Dawson 9.443
Bjellqvist 9.282
Wikipedia 9.663
Rodwell 9.56
Grimsley 9.545
Solomon 9.472
Lehninger 9.414
Nozaki 9.282
DTASelect 9.224
Thurlkill 9.282
EMBOSS 9.545
Sillero 9.428
Patrickios 4.355
IPC_peptide 9.458
IPC2_peptide 8.024
IPC2.peptide.svr19 7.754
Protein with the highest isoelectric point:
>tr|A0A2L0VZH6|A0A2L0VZH6_9VIRU RNA-dependent RNA polymerase OS=Gigaspora margarita mitovirus 2 OX=2082666 PE=4 SV=1
MM1 pKa = 7.61 TSFKK5 pKa = 10.59 YY6 pKa = 10.12 IKK8 pKa = 10.3 CSAPQTGWTFLSFRR22 pKa = 11.84 AFNTGSACLQVLLDD36 pKa = 5.06 HH37 pKa = 6.5 PLIWSVDD44 pKa = 3.26 WFKK47 pKa = 11.75 VKK49 pKa = 10.68 AGFYY53 pKa = 10.27 CIVDD57 pKa = 3.4 PTNPRR62 pKa = 11.84 EE63 pKa = 4.02 LIYY66 pKa = 10.87 LGEE69 pKa = 4.04 KK70 pKa = 10.32 DD71 pKa = 3.84 FRR73 pKa = 11.84 DD74 pKa = 4.97 FIKK77 pKa = 11.09 VMLSNRR83 pKa = 11.84 EE84 pKa = 3.84 PMVVLARR91 pKa = 11.84 AGEE94 pKa = 4.03 NQPKK98 pKa = 10.16 DD99 pKa = 3.98 SEE101 pKa = 4.29 ILTPKK106 pKa = 9.73 PKK108 pKa = 10.24 SPHH111 pKa = 4.81 STVFFRR117 pKa = 11.84 SFRR120 pKa = 11.84 VYY122 pKa = 11.1 SKK124 pKa = 10.37 FLRR127 pKa = 11.84 ALWSEE132 pKa = 3.78 MWEE135 pKa = 4.35 GKK137 pKa = 10.27 LVVPLEE143 pKa = 4.33 RR144 pKa = 11.84 NLKK147 pKa = 10.37 FIICNWGKK155 pKa = 7.75 TLLHH159 pKa = 6.36 WLGTSSSSRR168 pKa = 11.84 AQSQGLIQVSKK179 pKa = 11.04 YY180 pKa = 10.85 LMVVLKK186 pKa = 10.8 HH187 pKa = 6.35 RR188 pKa = 11.84 GATGLTIFLKK198 pKa = 10.94 VSFIALNKK206 pKa = 9.35 YY207 pKa = 9.82 LAGEE211 pKa = 4.14 PLKK214 pKa = 10.62 STFPLKK220 pKa = 10.58 CGMRR224 pKa = 11.84 LQGGLPSWLPAPVRR238 pKa = 11.84 LAIRR242 pKa = 11.84 QKK244 pKa = 8.72 SYY246 pKa = 10.97 KK247 pKa = 10.17 VIRR250 pKa = 11.84 LWSSLLYY257 pKa = 9.71 TYY259 pKa = 10.95 KK260 pKa = 10.85 GMYY263 pKa = 9.64 AKK265 pKa = 10.02 GVPSIASVLEE275 pKa = 4.17 PEE277 pKa = 4.87 WYY279 pKa = 9.31 TSSTRR284 pKa = 11.84 ATWLWGFQDD293 pKa = 4.04 FLANVYY299 pKa = 9.47 YY300 pKa = 10.57 RR301 pKa = 11.84 QLNWVGLDD309 pKa = 3.47 IPQSDD314 pKa = 4.2 QLPLLRR320 pKa = 11.84 TSAGPSGGTALAGVLRR336 pKa = 11.84 DD337 pKa = 3.29 LWVWRR342 pKa = 11.84 VFRR345 pKa = 11.84 HH346 pKa = 5.42 NRR348 pKa = 11.84 LHH350 pKa = 6.71 YY351 pKa = 10.66 SLEE354 pKa = 4.02 QILAYY359 pKa = 9.19 TGNGTALGFLRR370 pKa = 11.84 HH371 pKa = 5.73 GLAGAKK377 pKa = 9.43 PSQVFPLKK385 pKa = 9.18 VHH387 pKa = 6.72 KK388 pKa = 10.45 KK389 pKa = 9.27 GSCQEE394 pKa = 4.31 GYY396 pKa = 10.77 DD397 pKa = 3.09 IALYY401 pKa = 10.5 DD402 pKa = 3.56 GRR404 pKa = 11.84 KK405 pKa = 8.67 PLALARR411 pKa = 11.84 LHH413 pKa = 6.92 LIYY416 pKa = 10.38 EE417 pKa = 4.36 AAGKK421 pKa = 10.1 VRR423 pKa = 11.84 VIAILDD429 pKa = 3.85 YY430 pKa = 7.63 MTQWAFAPVHH440 pKa = 6.42 DD441 pKa = 4.27 FLMRR445 pKa = 11.84 LLATVDD451 pKa = 3.23 TDD453 pKa = 3.62 GTSDD457 pKa = 4.42 QNIGLQNFILRR468 pKa = 11.84 IGSRR472 pKa = 11.84 KK473 pKa = 9.86 GIYY476 pKa = 9.86 SSLDD480 pKa = 2.78 ISAATDD486 pKa = 3.86 TIPWQLYY493 pKa = 7.45 EE494 pKa = 4.33 VILGVMFDD502 pKa = 3.54 ARR504 pKa = 11.84 FARR507 pKa = 11.84 HH508 pKa = 5.05 YY509 pKa = 9.97 MRR511 pKa = 11.84 LLRR514 pKa = 11.84 EE515 pKa = 4.12 RR516 pKa = 11.84 GFLSPRR522 pKa = 11.84 EE523 pKa = 4.0 LPFEE527 pKa = 3.99 FVKK530 pKa = 10.89 YY531 pKa = 10.77 GCGQPMGALSSFPLLGLVHH550 pKa = 6.42 HH551 pKa = 7.27 AIVQYY556 pKa = 10.83 AAYY559 pKa = 8.25 EE560 pKa = 4.15 ANSFPFYY567 pKa = 10.38 DD568 pKa = 3.75 YY569 pKa = 11.22 CIVGDD574 pKa = 3.98 DD575 pKa = 3.63 LVLFEE580 pKa = 5.0 EE581 pKa = 5.01 DD582 pKa = 3.11 KK583 pKa = 11.18 AVPVATKK590 pKa = 9.62 YY591 pKa = 11.28 LSMCRR596 pKa = 11.84 YY597 pKa = 9.63 LGIRR601 pKa = 11.84 INKK604 pKa = 8.71 SKK606 pKa = 9.54 TYY608 pKa = 9.34 QSEE611 pKa = 4.43 VFFNFISRR619 pKa = 11.84 SFLNGIEE626 pKa = 4.01 VSPASMKK633 pKa = 10.69 SEE635 pKa = 3.97 LSVHH639 pKa = 5.4 TLGQRR644 pKa = 11.84 VEE646 pKa = 4.08 NTLRR650 pKa = 11.84 NCSRR654 pKa = 11.84 WSTGDD659 pKa = 2.97 SRR661 pKa = 11.84 SVMTRR666 pKa = 11.84 IIRR669 pKa = 11.84 FASDD673 pKa = 2.38 HH674 pKa = 5.87 WVWGTLSVSLSQGFLTSYY692 pKa = 10.18 VATVLASLLSPAGRR706 pKa = 11.84 FSSIFGVKK714 pKa = 9.77 EE715 pKa = 3.7 EE716 pKa = 4.08 GWIYY720 pKa = 9.99 WVAALRR726 pKa = 11.84 GSSIILSHH734 pKa = 7.21 DD735 pKa = 3.05 ASALTTHH742 pKa = 6.0 YY743 pKa = 10.91 LKK745 pKa = 11.08 NNYY748 pKa = 9.77 SKK750 pKa = 11.23 VLILIRR756 pKa = 11.84 TTCLVLRR763 pKa = 11.84 SEE765 pKa = 4.59 LEE767 pKa = 3.96 SLLVSGTKK775 pKa = 9.8 IADD778 pKa = 4.6 LYY780 pKa = 9.09 PSWMVKK786 pKa = 7.32 QPRR789 pKa = 11.84 GIRR792 pKa = 11.84 LLYY795 pKa = 10.17 QLFRR799 pKa = 11.84 EE800 pKa = 4.11 QRR802 pKa = 11.84 VNYY805 pKa = 10.15 AYY807 pKa = 10.81 FNFSSRR813 pKa = 11.84 EE814 pKa = 3.76 VRR816 pKa = 11.84 PLLGYY821 pKa = 9.43 PIAEE825 pKa = 4.19 SSSLLDD831 pKa = 3.69 EE832 pKa = 5.16 PDD834 pKa = 3.46 YY835 pKa = 11.33 EE836 pKa = 5.92 IITGMSHH843 pKa = 6.5 EE844 pKa = 4.62 EE845 pKa = 4.14 LIEE848 pKa = 4.27 SYY850 pKa = 10.96 IMEE853 pKa = 4.43 LLIYY857 pKa = 10.73 SMGYY861 pKa = 9.02 LVPRR865 pKa = 11.84 KK866 pKa = 8.86 FVPYY870 pKa = 9.97 LQEE873 pKa = 5.2 FYY875 pKa = 10.29 EE876 pKa = 4.22 AWSFIFSDD884 pKa = 4.03 NPDD887 pKa = 3.87 PEE889 pKa = 4.42 QLGQYY894 pKa = 7.54 LTQILEE900 pKa = 3.99 AVLAFKK906 pKa = 10.47 PDD908 pKa = 3.28 LDD910 pKa = 3.98 YY911 pKa = 11.63 RR912 pKa = 11.84 DD913 pKa = 3.59 VDD915 pKa = 3.83 VLSKK919 pKa = 8.86 VTRR922 pKa = 11.84 SEE924 pKa = 4.21 FGHH927 pKa = 5.58 TPRR930 pKa = 11.84 SSVINSNKK938 pKa = 9.3 FVYY941 pKa = 10.23 AKK943 pKa = 10.46 SGFANEE949 pKa = 3.92 LLQRR953 pKa = 11.84 LTIAWRR959 pKa = 11.84 MILSMEE965 pKa = 4.05 NLGINPEE972 pKa = 4.15 EE973 pKa = 4.21 TLKK976 pKa = 11.24 LPLPTSSFKK985 pKa = 10.74 EE986 pKa = 3.99
Molecular weight: 112.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.707
IPC2_protein 8.741
IPC_protein 8.639
Toseland 9.063
ProMoST 9.033
Dawson 9.443
Bjellqvist 9.282
Wikipedia 9.663
Rodwell 9.56
Grimsley 9.545
Solomon 9.472
Lehninger 9.414
Nozaki 9.282
DTASelect 9.224
Thurlkill 9.282
EMBOSS 9.545
Sillero 9.428
Patrickios 4.355
IPC_peptide 9.458
IPC2_peptide 8.024
IPC2.peptide.svr19 7.754
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
986
986
986
986.0
112.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.288 ± 0.0
1.116 ± 0.0
3.651 ± 0.0
4.868 ± 0.0
5.375 ± 0.0
6.288 ± 0.0
1.927 ± 0.0
5.578 ± 0.0
4.868 ± 0.0
12.779 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.13 ± 0.0
2.941 ± 0.0
4.767 ± 0.0
3.245 ± 0.0
6.085 ± 0.0
9.432 ± 0.0
4.767 ± 0.0
6.491 ± 0.0
2.535 ± 0.0
4.868 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here