Barley stripe mosaic virus (BSMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Hordeivirus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P04869|TGB2_BSMV Movement protein TGB2 OS=Barley stripe mosaic virus OX=12327 PE=1 SV=2
MM1 pKa = 7.68AMPHH5 pKa = 6.79PLEE8 pKa = 4.7CCCPQCLPSSEE19 pKa = 4.51SFPIYY24 pKa = 10.46GEE26 pKa = 3.92QEE28 pKa = 4.07IPCSEE33 pKa = 4.28TQAEE37 pKa = 4.35TTPVEE42 pKa = 3.99KK43 pKa = 9.75TVRR46 pKa = 11.84ANVLTDD52 pKa = 3.17ILDD55 pKa = 3.53DD56 pKa = 3.99HH57 pKa = 7.29YY58 pKa = 11.73YY59 pKa = 10.97AILASLFIIALWLLYY74 pKa = 9.78IYY76 pKa = 10.4LSSIPTEE83 pKa = 4.04TGPYY87 pKa = 9.81FYY89 pKa = 10.8QDD91 pKa = 3.47LNSVKK96 pKa = 9.98IYY98 pKa = 10.72GIGATNPEE106 pKa = 4.48VIAAIHH112 pKa = 6.19HH113 pKa = 4.96WQKK116 pKa = 11.06YY117 pKa = 8.24PFGEE121 pKa = 4.13SPMWGGLISVLSILLKK137 pKa = 10.58PLTLVFALSFFLLLSSKK154 pKa = 10.48RR155 pKa = 3.67

Molecular weight: 17.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P04868|TGB3_BSMV Movement protein TGB3 OS=Barley stripe mosaic virus OX=12327 PE=1 SV=1
MM1 pKa = 8.03DD2 pKa = 3.25MTKK5 pKa = 9.39TVEE8 pKa = 4.07EE9 pKa = 4.57KK10 pKa = 9.56KK11 pKa = 9.43TNGTDD16 pKa = 3.15SVKK19 pKa = 10.76GVFEE23 pKa = 4.28NSTIPKK29 pKa = 9.52VPTGQEE35 pKa = 3.63MGGDD39 pKa = 3.74GSSTSKK45 pKa = 10.8LKK47 pKa = 9.43EE48 pKa = 3.98TLKK51 pKa = 11.09VADD54 pKa = 3.99QTPLSVDD61 pKa = 2.98NGAKK65 pKa = 10.12SKK67 pKa = 10.84LDD69 pKa = 3.34SSDD72 pKa = 3.38RR73 pKa = 11.84QVPGVADD80 pKa = 3.68QTPLSVDD87 pKa = 2.98NGAKK91 pKa = 10.12SKK93 pKa = 10.84LDD95 pKa = 3.34SSDD98 pKa = 3.39RR99 pKa = 11.84QVPGPEE105 pKa = 4.03LKK107 pKa = 10.8PNVKK111 pKa = 9.88KK112 pKa = 10.8SKK114 pKa = 10.08KK115 pKa = 10.03KK116 pKa = 10.41RR117 pKa = 11.84IQKK120 pKa = 8.88PAQPSGPNDD129 pKa = 3.45LKK131 pKa = 11.36GGTKK135 pKa = 10.28GSSQVGEE142 pKa = 4.19NVSEE146 pKa = 4.21NYY148 pKa = 9.55TGISKK153 pKa = 10.04EE154 pKa = 3.99AAKK157 pKa = 10.32QKK159 pKa = 10.65QKK161 pKa = 9.58TPKK164 pKa = 8.97SVKK167 pKa = 9.0MQSNLADD174 pKa = 3.36KK175 pKa = 10.72FKK177 pKa = 11.41ANDD180 pKa = 3.36TRR182 pKa = 11.84RR183 pKa = 11.84SEE185 pKa = 4.85LINKK189 pKa = 7.42FQQFVHH195 pKa = 5.52EE196 pKa = 4.59TCLKK200 pKa = 10.69SDD202 pKa = 3.89FEE204 pKa = 4.61YY205 pKa = 10.4TGRR208 pKa = 11.84QYY210 pKa = 10.96FRR212 pKa = 11.84ARR214 pKa = 11.84SNFFEE219 pKa = 4.31MIKK222 pKa = 10.44LASLYY227 pKa = 10.48DD228 pKa = 3.56KK229 pKa = 10.82HH230 pKa = 8.26LKK232 pKa = 9.93EE233 pKa = 4.47CMARR237 pKa = 11.84ACTLEE242 pKa = 3.76RR243 pKa = 11.84EE244 pKa = 4.05RR245 pKa = 11.84LKK247 pKa = 10.99RR248 pKa = 11.84KK249 pKa = 9.57LLLVRR254 pKa = 11.84ALKK257 pKa = 10.01PAVDD261 pKa = 4.64FLTGIISGVPGSGKK275 pKa = 8.33STIVRR280 pKa = 11.84TLLKK284 pKa = 10.86GEE286 pKa = 4.48FPAVCALANPALMNDD301 pKa = 3.38YY302 pKa = 11.01SGIEE306 pKa = 4.06GVYY309 pKa = 10.94GLDD312 pKa = 4.2DD313 pKa = 4.69LLLSAVPITSDD324 pKa = 3.63LLIIDD329 pKa = 5.26EE330 pKa = 4.37YY331 pKa = 10.97TLAEE335 pKa = 4.09SAEE338 pKa = 3.95ILLLQRR344 pKa = 11.84RR345 pKa = 11.84LRR347 pKa = 11.84ASMVLLVGDD356 pKa = 4.39VAQGKK361 pKa = 7.86ATTASSIEE369 pKa = 4.37YY370 pKa = 8.43LTLPVIYY377 pKa = 9.94RR378 pKa = 11.84SEE380 pKa = 3.83TTYY383 pKa = 11.49RR384 pKa = 11.84LGQEE388 pKa = 4.16TASLCSKK395 pKa = 10.02QGNRR399 pKa = 11.84MVSKK403 pKa = 10.59GGRR406 pKa = 11.84DD407 pKa = 3.25TVIITDD413 pKa = 3.63YY414 pKa = 11.28DD415 pKa = 3.95GEE417 pKa = 4.27TDD419 pKa = 3.39EE420 pKa = 4.62TEE422 pKa = 3.93KK423 pKa = 11.37NIAFTVDD430 pKa = 2.96TVRR433 pKa = 11.84DD434 pKa = 4.03VKK436 pKa = 11.31DD437 pKa = 3.59CGYY440 pKa = 10.99DD441 pKa = 3.1CALAIDD447 pKa = 4.19VQGKK451 pKa = 9.17EE452 pKa = 4.08FDD454 pKa = 3.71SVTLFLRR461 pKa = 11.84NEE463 pKa = 3.95DD464 pKa = 3.56RR465 pKa = 11.84KK466 pKa = 10.89ALADD470 pKa = 3.25KK471 pKa = 10.58HH472 pKa = 6.21LRR474 pKa = 11.84LVALSRR480 pKa = 11.84HH481 pKa = 5.11KK482 pKa = 10.93SKK484 pKa = 11.22LIIRR488 pKa = 11.84ADD490 pKa = 3.39AEE492 pKa = 3.77IRR494 pKa = 11.84QAFLTGDD501 pKa = 3.21IDD503 pKa = 4.76LSSKK507 pKa = 10.6ASNSHH512 pKa = 6.61RR513 pKa = 11.84YY514 pKa = 7.87SAKK517 pKa = 9.79PDD519 pKa = 3.68EE520 pKa = 4.29DD521 pKa = 4.02HH522 pKa = 7.41SWFKK526 pKa = 11.42AKK528 pKa = 10.47

Molecular weight: 58.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3148

153

1139

449.7

50.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.417 ± 0.515

2.51 ± 0.489

6.163 ± 0.485

6.163 ± 0.298

4.416 ± 0.474

5.527 ± 0.537

2.097 ± 0.24

5.464 ± 0.448

6.766 ± 0.66

9.466 ± 0.447

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.425

3.59 ± 0.238

4.479 ± 0.332

3.272 ± 0.326

5.813 ± 0.445

8.482 ± 0.68

5.273 ± 0.342

7.211 ± 0.648

0.985 ± 0.197

3.748 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski