Azospirillum lipoferum (strain 4B)
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6062 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G7ZEJ4|G7ZEJ4_AZOL4 Acyl-CoA dehydrogenase OS=Azospirillum lipoferum (strain 4B) OX=862719 GN=fadE PE=3 SV=1
MM1 pKa = 7.74 AIEE4 pKa = 4.84 TIYY7 pKa = 9.99 DD8 pKa = 3.47 TLAAQGGTVRR18 pKa = 11.84 LDD20 pKa = 3.43 DD21 pKa = 4.81 ASLGTIGLDD30 pKa = 3.12 ALLAATGLPAAIAVVLPGALPQPDD54 pKa = 4.05 GDD56 pKa = 3.85 TLVFSGTSGFLAGTVQSWGGSSGFLDD82 pKa = 4.36 GAAFGVTVTIITSGSDD98 pKa = 3.23 EE99 pKa = 4.49 ASLEE103 pKa = 4.28 VTVALPAGWVLGDD116 pKa = 3.61 SFYY119 pKa = 11.42 GFSGTTFTTDD129 pKa = 2.66 ALRR132 pKa = 11.84 YY133 pKa = 7.59 PGLVFGYY140 pKa = 10.25 NGGGVSDD147 pKa = 4.14 GLILSAEE154 pKa = 4.08 IFATGPLTPLPLLADD169 pKa = 4.03 MPSPAPISGPVVLSEE184 pKa = 4.12 WGPAFTLSGALGTIRR199 pKa = 11.84 LMFDD203 pKa = 3.87 PLPLTNASYY212 pKa = 11.23 RR213 pKa = 11.84 LTLGYY218 pKa = 9.46 EE219 pKa = 3.85 QSSYY223 pKa = 11.58 TDD225 pKa = 3.51 TTDD228 pKa = 2.97 TAYY231 pKa = 10.81 LPSIGNDD238 pKa = 3.46 FVADD242 pKa = 4.32 CGAINDD248 pKa = 4.12 TTLVMTVTLSRR259 pKa = 11.84 TNVVTLSFSGAAGSNPVANQDD280 pKa = 3.31 VLIPLFPNSAGFPGSLTSSLFAGMVPGIILFGATGDD316 pKa = 3.88 PSTGAMSSTFGSLGLIEE333 pKa = 4.43 PWIPIPAFPDD343 pKa = 3.75 FQVVSLEE350 pKa = 4.24 MTWSYY355 pKa = 11.85 VYY357 pKa = 10.64 GPPSSLSGSIGATLDD372 pKa = 3.18 LGGYY376 pKa = 9.94 YY377 pKa = 10.07 FDD379 pKa = 6.32 LDD381 pKa = 3.24 IEE383 pKa = 4.46 YY384 pKa = 9.31 PSLSFAGTLQNTVTLSFAEE403 pKa = 4.45 VNSAMLSVLDD413 pKa = 3.85 VQAPANADD421 pKa = 3.25 VGTLTFSGFGIAVDD435 pKa = 4.82 LDD437 pKa = 3.86 ADD439 pKa = 4.07 TLSFQATADD448 pKa = 3.44 IQLTLAGLAISLSATTLNLDD468 pKa = 4.01 YY469 pKa = 10.92 GWSANAAVFTVDD481 pKa = 4.09 GVFAVNSWQFSLSALLEE498 pKa = 4.21 SAGMRR503 pKa = 11.84 FSGQLLQAQTVVVSDD518 pKa = 3.67 VVGQLLGPVVTPDD531 pKa = 3.4 QFSLALTVEE540 pKa = 4.41 AFSLTIDD547 pKa = 4.33 TITDD551 pKa = 3.14 AMAVSGAVGWSLTVCGQTMGGDD573 pKa = 3.49 FTIDD577 pKa = 3.09 VSSPDD582 pKa = 4.24 SGSSWAGTIGGGITIADD599 pKa = 3.49 IAFGMDD605 pKa = 3.92 YY606 pKa = 10.89 SWGPSLGLLKK616 pKa = 10.87 GSFSAGQTYY625 pKa = 10.72 DD626 pKa = 2.39 WTAFVDD632 pKa = 4.29 AFGATQVPLPAPIPTAPDD650 pKa = 3.77 LDD652 pKa = 5.56 PITVGFDD659 pKa = 3.19 YY660 pKa = 11.08 DD661 pKa = 3.76 FEE663 pKa = 6.55 SDD665 pKa = 3.71 VFTLTGVAAGGDD677 pKa = 3.18 ASMAGFFSAVPALYY691 pKa = 9.85 TGTGKK696 pKa = 8.66 TGFAAGIQVEE706 pKa = 4.22 LTPGFFAGMNSALAPFDD723 pKa = 4.92 DD724 pKa = 5.09 FGLSSVIAFTVANFTNPSFLLPIATQSYY752 pKa = 9.44 FPGVCAGMTFSAAFSWPEE770 pKa = 4.02 CPDD773 pKa = 3.56 DD774 pKa = 3.72 LTRR777 pKa = 11.84 TSVSLMTGSVSAISVMIGFYY797 pKa = 10.57 DD798 pKa = 3.74 IAGGWKK804 pKa = 9.21 FQFAGTMSDD813 pKa = 2.69 IALNLGAGIDD823 pKa = 3.72 IEE825 pKa = 4.7 SVSLALAMTEE835 pKa = 3.83 TGIDD839 pKa = 2.62 VDD841 pKa = 4.17 YY842 pKa = 11.27 QVLFSILVSIDD853 pKa = 3.71 PGSGWVDD860 pKa = 3.05 SGDD863 pKa = 3.74 GTKK866 pKa = 10.18 SDD868 pKa = 3.43 QLLFDD873 pKa = 4.05 GQLEE877 pKa = 4.37 FEE879 pKa = 4.4 ATSAGGVGGFAAIFMSGTWHH899 pKa = 6.6 EE900 pKa = 4.37 PFGIPDD906 pKa = 4.33 LSVADD911 pKa = 4.55 LGLLLGVAEE920 pKa = 4.35 QGGEE924 pKa = 4.01 PVPSFGLTGFLAYY937 pKa = 9.72 PPAGVEE943 pKa = 4.02 AFVAIYY949 pKa = 10.24 FDD951 pKa = 3.96 GANPDD956 pKa = 3.52 DD957 pKa = 4.08 SLFIGSMSEE966 pKa = 3.97 VAFLQFAEE974 pKa = 4.2 MMAWAAAGVEE984 pKa = 4.64 VPSLLQSVLEE994 pKa = 4.44 TYY996 pKa = 10.25 GVYY999 pKa = 10.36 PIPFDD1004 pKa = 4.28 LSTATTTLTDD1014 pKa = 3.64 ADD1016 pKa = 4.15 FAQLNDD1022 pKa = 3.67 YY1023 pKa = 9.27 VVPNAFFALVAEE1035 pKa = 5.53 LGQPMQKK1042 pKa = 9.76 PAAGALYY1049 pKa = 8.3 QTLTVILGDD1058 pKa = 4.03 ADD1060 pKa = 3.76 NPDD1063 pKa = 3.88 VNTEE1067 pKa = 3.58 WFIADD1072 pKa = 4.08 FQSGQIFHH1080 pKa = 7.26 LVRR1083 pKa = 11.84 TGTGSPPYY1091 pKa = 9.04 TLAMDD1096 pKa = 3.9 PWLYY1100 pKa = 10.73 VVPNTVTLGLVTYY1113 pKa = 8.58 QQGYY1117 pKa = 8.76 GFGGRR1122 pKa = 11.84 LKK1124 pKa = 11.06 LPGLEE1129 pKa = 3.86 LDD1131 pKa = 4.11 IVAEE1135 pKa = 4.18 LAPTLGLKK1143 pKa = 9.91 VDD1145 pKa = 4.2 ADD1147 pKa = 3.81 APEE1150 pKa = 5.3 PILYY1154 pKa = 8.85 PAAIGNTPVPPLLVITAATDD1174 pKa = 3.26 DD1175 pKa = 3.89 TLGPRR1180 pKa = 11.84 VSLYY1184 pKa = 9.66 TYY1186 pKa = 10.31 QVGDD1190 pKa = 4.08 IPPHH1194 pKa = 6.38 FYY1196 pKa = 11.11 LDD1198 pKa = 3.61 AAVSLLNMAKK1208 pKa = 9.83 ISTEE1212 pKa = 3.44 ISILSSGLSFYY1223 pKa = 11.35 LEE1225 pKa = 4.53 LSTALDD1231 pKa = 3.57 VLDD1234 pKa = 5.41 ARR1236 pKa = 11.84 MTCLVANEE1244 pKa = 4.27 GFSANFDD1251 pKa = 3.64 LSLDD1255 pKa = 3.98 LDD1257 pKa = 3.85 VDD1259 pKa = 4.01 TDD1261 pKa = 3.97 PVKK1264 pKa = 10.72 VAGVTVIPSIHH1275 pKa = 6.77 LNTGFGLSLAVSFPLTSGDD1294 pKa = 4.03 SNVQAAFKK1302 pKa = 10.96 GKK1304 pKa = 10.14 ISFNWGDD1311 pKa = 3.36 QSFSAGIDD1319 pKa = 3.6 FKK1321 pKa = 11.54 LSDD1324 pKa = 4.43 LADD1327 pKa = 4.05 LTTVIADD1334 pKa = 3.38 WVVKK1338 pKa = 10.45 NAGALFEE1345 pKa = 5.0 NLLNDD1350 pKa = 4.75 FIQWARR1356 pKa = 11.84 AMINGLFSFAAAAAASALKK1375 pKa = 10.64 ALGAAYY1381 pKa = 9.77 DD1382 pKa = 3.69 AAAQLMAEE1390 pKa = 4.32 IGYY1393 pKa = 8.14 TLDD1396 pKa = 3.36 EE1397 pKa = 4.61 VYY1399 pKa = 10.89 DD1400 pKa = 4.39 AIKK1403 pKa = 10.7 AVFAVAEE1410 pKa = 4.11 EE1411 pKa = 4.15 LLINALTKK1419 pKa = 10.53 AFHH1422 pKa = 6.3 IQFSYY1427 pKa = 11.08 AALSQQVGPATTATAPSTLLDD1448 pKa = 3.45 WTEE1451 pKa = 3.8 NTAAPAVAAGEE1462 pKa = 4.43 WLSIMQPDD1470 pKa = 3.78 SGVSGVFAASLFDD1483 pKa = 4.46 TISLDD1488 pKa = 3.74 IALADD1493 pKa = 4.07 PGLYY1497 pKa = 10.52 ALSFKK1502 pKa = 11.01 GFGLSATMPYY1512 pKa = 10.47 QRR1514 pKa = 11.84 VSDD1517 pKa = 3.9 SLGVFSLEE1525 pKa = 4.19 MPSSAEE1531 pKa = 3.72 LLTIGFGPFGFRR1543 pKa = 11.84 NPALTIAIYY1552 pKa = 10.58 TDD1554 pKa = 3.3 GTYY1557 pKa = 10.78 LFDD1560 pKa = 4.37 YY1561 pKa = 9.78 GYY1563 pKa = 9.63 PDD1565 pKa = 4.32 DD1566 pKa = 5.71 GDD1568 pKa = 3.66 WSRR1571 pKa = 11.84 SAALVTPIFSGQGGFQFTSLGNSSLPAGWSPVTLALGVAAAMRR1614 pKa = 11.84 IALDD1618 pKa = 3.72 GSLDD1622 pKa = 3.65 LGMLSGGWSLTYY1634 pKa = 10.38 EE1635 pKa = 4.42 GGFTAIAGYY1644 pKa = 9.84 GAGLSPTGSAVIDD1657 pKa = 3.81 PSQPAEE1663 pKa = 4.02 FQGTLTLSFSGALYY1677 pKa = 10.38 GSVDD1681 pKa = 3.99 FGIVSAAVHH1690 pKa = 6.13 VDD1692 pKa = 3.23 LSGTMGCGLTVGSSVALSLNVLVSVGVKK1720 pKa = 9.69 VRR1722 pKa = 11.84 IKK1724 pKa = 10.83 LGLFKK1729 pKa = 10.1 ITISFKK1735 pKa = 10.32 FSMHH1739 pKa = 6.06 VDD1741 pKa = 3.63 VGPATLPWPSTAMPSSAVTSPLEE1764 pKa = 4.24 PIAWAGTPSSRR1775 pKa = 11.84 LLSSRR1780 pKa = 11.84 TMAATAAGASLPALGLWFIPEE1801 pKa = 3.88 LTITYY1806 pKa = 9.49 DD1807 pKa = 3.58 GKK1809 pKa = 11.5 GSGTPAMLFNLAIPYY1824 pKa = 9.15 TEE1826 pKa = 5.28 ADD1828 pKa = 3.91 PGCMFNLLVQSLLQWALSLDD1848 pKa = 3.92 GEE1850 pKa = 4.6 AGEE1853 pKa = 4.47 TTAVSRR1859 pKa = 11.84 DD1860 pKa = 3.13 RR1861 pKa = 11.84 LEE1863 pKa = 3.97 RR1864 pKa = 11.84 LTRR1867 pKa = 11.84 RR1868 pKa = 11.84 LKK1870 pKa = 9.58 TKK1872 pKa = 9.7 PRR1874 pKa = 11.84 QSRR1877 pKa = 11.84 LYY1879 pKa = 10.99 ADD1881 pKa = 5.03 ADD1883 pKa = 4.03 DD1884 pKa = 5.66 PDD1886 pKa = 3.95 TLLTYY1891 pKa = 10.93 DD1892 pKa = 4.95 LLVQFLQDD1900 pKa = 2.77 WFSAVTVTVPVSDD1913 pKa = 4.29 ASAVAFPMIPDD1924 pKa = 3.84 LVAWGGVEE1932 pKa = 4.13 GGTSSSLCDD1941 pKa = 3.34 FAQYY1945 pKa = 10.57 NRR1947 pKa = 11.84 QPTSYY1952 pKa = 9.86 EE1953 pKa = 3.69 DD1954 pKa = 4.61 AIAATFATFKK1964 pKa = 10.92 LFAAPEE1970 pKa = 4.22 TTAPLAMAEE1979 pKa = 4.15 DD1980 pKa = 5.3 DD1981 pKa = 4.16 GTASLATYY1989 pKa = 10.75 AFLAYY1994 pKa = 9.98 FEE1996 pKa = 4.75 YY1997 pKa = 10.88 LLQGGAGEE2005 pKa = 4.75 ALDD2008 pKa = 3.84 LTGAAGLDD2016 pKa = 3.62 QIGAADD2022 pKa = 3.75 WAALAFDD2029 pKa = 3.95 QTASGITVSFRR2040 pKa = 11.84 GGVQLPANGEE2050 pKa = 4.17 AGQDD2054 pKa = 3.49 MAPLYY2059 pKa = 10.42 QLTGQQMTLATIATPPTAISLSLARR2084 pKa = 11.84 GSGGGWITLDD2094 pKa = 3.71 PQPVAGFSQQAVSDD2108 pKa = 3.81 ILGLTVSAQLTRR2120 pKa = 11.84 VTQTALTAILATYY2133 pKa = 9.56 PFGSFTAYY2141 pKa = 10.56 APTDD2145 pKa = 3.67 GKK2147 pKa = 11.02 SATLAAIPPALAAAPGAVTLATGVPGQSGTLDD2179 pKa = 4.26 GDD2181 pKa = 4.17 VQWASLLTLTLRR2193 pKa = 11.84 QVVAAGGGIDD2203 pKa = 3.39 QTVYY2207 pKa = 10.67 TLTAVDD2213 pKa = 4.12 SSQVGVIAQLIVASAAGTALTLTLLVPTGDD2243 pKa = 3.61 GGYY2246 pKa = 9.84 AACAAAADD2254 pKa = 4.04 ATTLLRR2260 pKa = 11.84 TNLSTEE2266 pKa = 4.15 SQPQLDD2272 pKa = 3.72 TAARR2276 pKa = 11.84 SQTEE2280 pKa = 4.07 EE2281 pKa = 3.69 ASATGGTDD2289 pKa = 3.24 PVAAALTDD2297 pKa = 3.67 AANALAILLALGEE2310 pKa = 4.39 TNAAGYY2316 pKa = 10.18 YY2317 pKa = 9.94 LALDD2321 pKa = 4.01 EE2322 pKa = 5.45 ADD2324 pKa = 4.08 SGLAGLFTTSNTATAALLVARR2345 pKa = 11.84 GSAVNGTATTSSAATDD2361 pKa = 3.44 NVLLVDD2367 pKa = 4.68 QIAASDD2373 pKa = 3.91 LTSLGLFATSTSLVGYY2389 pKa = 9.68 QPAYY2393 pKa = 9.07 PPGVLPVTVTRR2404 pKa = 11.84 PNPEE2408 pKa = 3.97 TADD2411 pKa = 3.61 SDD2413 pKa = 4.48 EE2414 pKa = 4.45 DD2415 pKa = 4.6 QSWLDD2420 pKa = 3.23 QLYY2423 pKa = 11.0 GLFTVAISGSAYY2435 pKa = 10.43 ADD2437 pKa = 3.39 LGYY2440 pKa = 11.18 APACSPADD2448 pKa = 4.07 DD2449 pKa = 4.85 GAEE2452 pKa = 3.61 TWTYY2456 pKa = 10.81 FLSLPLTNLLAGGPSDD2472 pKa = 4.15 NPYY2475 pKa = 10.76 AAIGTTPTVTTQIRR2489 pKa = 11.84 DD2490 pKa = 3.67 CFGNPVTTNLTAFAVPQTYY2509 pKa = 10.92 NDD2511 pKa = 3.67 VLVGPGAWVGSTLNWTPTTKK2531 pKa = 10.63 AGTLTVTLAVDD2542 pKa = 4.81 KK2543 pKa = 10.57 ATLDD2547 pKa = 3.81 NNLDD2551 pKa = 3.8 TAQPLYY2557 pKa = 10.93 AQAAWQLAEE2566 pKa = 4.13 TGDD2569 pKa = 3.36 IEE2571 pKa = 4.78 FRR2573 pKa = 11.84 LSSSLDD2579 pKa = 3.24 TGGAQPLAAAGQQAITAYY2597 pKa = 9.71 VAAVLDD2603 pKa = 4.18 YY2604 pKa = 11.37 LDD2606 pKa = 4.65 GASTTLPAAVEE2617 pKa = 3.99 LTYY2620 pKa = 11.03 GIASPVAALPPVAVDD2635 pKa = 3.32 VALILARR2642 pKa = 11.84 VAHH2645 pKa = 6.27 VDD2647 pKa = 3.11 ASTAALLPQVASIATPATADD2667 pKa = 3.77 LSAGLTTFATIFQTAFPNLCLATGSGSGSNQLWAVAAGLLQPAISGTPTYY2717 pKa = 9.8 FAPAPFYY2724 pKa = 10.83 TSLQSGTTPVPQYY2737 pKa = 11.41 GAFTTSSWTSADD2749 pKa = 3.14 QSYY2752 pKa = 10.7 AAVDD2756 pKa = 3.84 CDD2758 pKa = 4.18 QLFQSICGTIDD2769 pKa = 3.51 LLLGGSYY2776 pKa = 10.22 AAAAFRR2782 pKa = 11.84 ANPDD2786 pKa = 3.08 GYY2788 pKa = 9.17 RR2789 pKa = 11.84 TLVEE2793 pKa = 3.86 GRR2795 pKa = 11.84 AAIAQLYY2802 pKa = 10.39 SDD2804 pKa = 4.81 NQLSWLFTEE2813 pKa = 4.56 QAGAADD2819 pKa = 5.85 DD2820 pKa = 3.83 PTLWGSRR2827 pKa = 11.84 EE2828 pKa = 4.18 SFSSNLQNALANAYY2842 pKa = 10.21 VLDD2845 pKa = 4.22 GVLAFDD2851 pKa = 5.33 ADD2853 pKa = 3.99 WAKK2856 pKa = 11.06 LPAGATATGLTLWGEE2871 pKa = 4.32 VGPGNGSTALVTTTASLTLDD2891 pKa = 4.26 DD2892 pKa = 4.61 SASPATTLYY2901 pKa = 10.58 YY2902 pKa = 10.56 DD2903 pKa = 3.97 FRR2905 pKa = 11.84 PGEE2908 pKa = 4.02 LSADD2912 pKa = 3.53 ASLSVPLSYY2921 pKa = 10.5 RR2922 pKa = 11.84 VTHH2925 pKa = 6.26 VALTDD2930 pKa = 3.28 TGNGVSIWLALALPATVSIGTGDD2953 pKa = 3.71 VEE2955 pKa = 4.53 VPLIDD2960 pKa = 4.4 RR2961 pKa = 11.84 FFPTAPTLQTQSADD2975 pKa = 3.13 ATTVADD2981 pKa = 4.28 ADD2983 pKa = 4.54 FSQLFQWRR2991 pKa = 11.84 YY2992 pKa = 7.36 SAGMLLEE2999 pKa = 4.72 PVAQDD3004 pKa = 3.2 TTTLTIRR3011 pKa = 11.84 YY3012 pKa = 7.96 NRR3014 pKa = 11.84 QSADD3018 pKa = 3.27 QAALKK3023 pKa = 10.82 AMAAVTPVLTAAQALMRR3040 pKa = 11.84 FQAGYY3045 pKa = 11.33 AEE3047 pKa = 4.66 IEE3049 pKa = 4.28 PVLPDD3054 pKa = 4.51 LLAADD3059 pKa = 3.82 VNAANIVAALADD3071 pKa = 4.27 LVLAIQINSDD3081 pKa = 3.08 WAGSGATAPILAAAIPTASTEE3102 pKa = 4.12 LSLSLAAEE3110 pKa = 4.23 EE3111 pKa = 4.35 EE3112 pKa = 4.41 DD3113 pKa = 3.97 GKK3115 pKa = 10.11 VTGRR3119 pKa = 11.84 WILTVATTGDD3129 pKa = 3.28 GTLPGTLSALPLTADD3144 pKa = 3.91 GTAQPATTVLGIEE3157 pKa = 4.45 PLQIAYY3163 pKa = 9.48 IPEE3166 pKa = 4.88 DD3167 pKa = 3.64 DD3168 pKa = 3.98 AASVLVQVTLDD3179 pKa = 3.68 GLDD3182 pKa = 3.16 LRR3184 pKa = 11.84 LYY3186 pKa = 10.31 QNAVTEE3192 pKa = 4.69 AYY3194 pKa = 7.89 VTRR3197 pKa = 11.84 NASLLTGYY3205 pKa = 6.31 TTEE3208 pKa = 3.81 GSYY3211 pKa = 11.23 VFVTATVAFPQPVAPYY3227 pKa = 8.71 ITGTGTGTGYY3237 pKa = 11.31 NLAEE3241 pKa = 4.43 VVATPVNATLANWLAALLSEE3261 pKa = 4.64 VAGSAAHH3268 pKa = 6.6 PRR3270 pKa = 11.84 LALTLGYY3277 pKa = 10.21 RR3278 pKa = 11.84 FSQYY3282 pKa = 10.42 TGTDD3286 pKa = 3.11 AEE3288 pKa = 4.71 GSIEE3292 pKa = 4.24 TIQAFQPVTLSRR3304 pKa = 11.84 TVLLDD3309 pKa = 3.19 TGSGGEE3315 pKa = 4.05 VTVATFAQALADD3327 pKa = 5.48 AFIGWQDD3334 pKa = 3.26 SHH3336 pKa = 8.25 PLDD3339 pKa = 3.73 LHH3341 pKa = 6.12 TGQLVVTLSLYY3352 pKa = 10.22 SAQGSIGPALLQAPNLWLALAAIEE3376 pKa = 4.43 NEE3378 pKa = 4.23 TRR3380 pKa = 3.32
Molecular weight: 348.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.617
IPC_protein 3.681
Toseland 3.439
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.503
Grimsley 3.338
Solomon 3.681
Lehninger 3.643
Nozaki 3.783
DTASelect 4.075
Thurlkill 3.49
EMBOSS 3.63
Sillero 3.808
Patrickios 0.566
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.726
Protein with the highest isoelectric point:
>tr|G7Z381|G7Z381_AZOL4 Uncharacterized protein OS=Azospirillum lipoferum (strain 4B) OX=862719 GN=AZOLI_0494 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 8.96 IVRR12 pKa = 11.84 KK13 pKa = 9.24 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.55 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.3 VIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.91 VLSAA44 pKa = 4.11
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6062
0
6062
1974684
17
6548
325.7
35.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.193 ± 0.05
0.867 ± 0.012
5.872 ± 0.037
5.463 ± 0.032
3.3 ± 0.021
8.941 ± 0.06
2.002 ± 0.018
4.433 ± 0.023
2.758 ± 0.027
10.617 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.428 ± 0.016
2.328 ± 0.021
5.632 ± 0.037
2.977 ± 0.02
7.713 ± 0.045
5.121 ± 0.035
5.465 ± 0.054
7.607 ± 0.026
1.289 ± 0.014
1.993 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here