Crenothrix polyspora
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3943 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R4H357|A0A1R4H357_9GAMM Cupin_2 domain-containing protein OS=Crenothrix polyspora OX=360316 GN=CRENPOLYSF1_150087 PE=4 SV=1
MM1 pKa = 7.08 GTGTADD7 pKa = 3.42 AVTGAYY13 pKa = 10.19 SIVPSVVLVDD23 pKa = 3.77 GAVLSVTATDD33 pKa = 3.41 AAGNVSPVAMTTVDD47 pKa = 3.25 ATAPALPTINATDD60 pKa = 4.06 GSSITGVAEE69 pKa = 4.22 AGSVVTLKK77 pKa = 10.83 DD78 pKa = 3.17 AAGVLIGTATADD90 pKa = 3.82 ALTGAYY96 pKa = 10.02 SIVPSVVLVDD106 pKa = 4.01 GALLSVTATDD116 pKa = 3.35 AAGNVSPVATATVNAAGDD134 pKa = 3.7 ITPPIVPIINATDD147 pKa = 4.27 GSPITGVAEE156 pKa = 4.21 AGSFVTIKK164 pKa = 10.59 DD165 pKa = 3.23 ALGAVIGTGTADD177 pKa = 3.57 AVTGAYY183 pKa = 10.11 SIIPSTVPADD193 pKa = 3.62 GAVLSVIATDD203 pKa = 3.4 AAGNVSPVATTTVDD217 pKa = 3.1 ATVPALPTINATDD230 pKa = 4.44 GSPIAGVAEE239 pKa = 4.34 AGSFVTIKK247 pKa = 10.59 DD248 pKa = 3.23 ALGAVIGTGTADD260 pKa = 3.57 AVTGAYY266 pKa = 10.14 SIVPSTVPVDD276 pKa = 3.62 GAVLSVTATDD286 pKa = 3.39 AAGNVSSVATTIVDD300 pKa = 3.65 ATAPTSIVSAIGDD313 pKa = 3.77 TSKK316 pKa = 9.55 TAAEE320 pKa = 4.11 ATHH323 pKa = 6.35 AAGVVTVNGEE333 pKa = 4.11 TGSTSVLTLVGVNGTVTKK351 pKa = 10.11 TIANDD356 pKa = 3.65 GTALPVVLSAADD368 pKa = 3.73 LTILGDD374 pKa = 3.66 GAVEE378 pKa = 4.19 VTTITTDD385 pKa = 3.1 VAGNAITTADD395 pKa = 3.5 VSDD398 pKa = 3.96 GDD400 pKa = 4.09 FVLDD404 pKa = 4.04 TVAPMPVISAIGDD417 pKa = 3.69 VAKK420 pKa = 9.47 TAAEE424 pKa = 4.06 ATDD427 pKa = 3.35 AAGVVTVTGEE437 pKa = 4.02 TGSTSVVTFVGVNGTVTKK455 pKa = 10.34 TITNDD460 pKa = 2.93 GTALPVVLSAADD472 pKa = 3.85 LTTLGDD478 pKa = 3.62 GAVEE482 pKa = 4.29 VTTVTTDD489 pKa = 2.97 AAGNPTTTADD499 pKa = 3.39 VSDD502 pKa = 4.1 GDD504 pKa = 4.23 FVLDD508 pKa = 4.15 TAAPTPTVTAIGDD521 pKa = 3.73 AAKK524 pKa = 9.32 TVAEE528 pKa = 4.27 ATDD531 pKa = 3.19 AAGIVTVNGEE541 pKa = 4.22 TGSTAVVTFVGVNGTVTKK559 pKa = 10.11 TIANDD564 pKa = 3.65 GTALPVVLTAADD576 pKa = 4.18 LATLGDD582 pKa = 3.82 GAVEE586 pKa = 4.29 VTTVTTDD593 pKa = 2.97 AAGNPTTTADD603 pKa = 3.42 VSDD606 pKa = 4.22 GDD608 pKa = 4.44 FILDD612 pKa = 3.89 IIAPIVTAPVVSYY625 pKa = 10.64 TDD627 pKa = 3.35 NNIPADD633 pKa = 4.04 QIDD636 pKa = 5.42 LIVQTFGSGTGRR648 pKa = 11.84 ASFASAGVIATTSYY662 pKa = 10.22 IYY664 pKa = 10.69 SAGAVPDD671 pKa = 3.68 GDD673 pKa = 3.9 YY674 pKa = 10.73 TLSNTVSGLEE684 pKa = 4.15 TWWASANVTVPAAQDD699 pKa = 3.42 LAFRR703 pKa = 11.84 DD704 pKa = 3.51 HH705 pKa = 6.72 TGDD708 pKa = 3.27 VNGRR712 pKa = 11.84 FLAVNASAAPGEE724 pKa = 5.04 FYY726 pKa = 10.6 RR727 pKa = 11.84 QPITVSDD734 pKa = 3.51 AGDD737 pKa = 3.47 YY738 pKa = 10.42 KK739 pKa = 11.04 VAAFLASANNAPINANVTIKK759 pKa = 10.51 IVDD762 pKa = 3.56 SLGNIVASIDD772 pKa = 3.51 TGDD775 pKa = 4.16 LPDD778 pKa = 3.66 YY779 pKa = 10.56 TNAASAWRR787 pKa = 11.84 EE788 pKa = 3.77 YY789 pKa = 11.46 SLMANLPAGSYY800 pKa = 11.1 NFVLVNNAPGGSGNDD815 pKa = 3.14 IFIDD819 pKa = 4.66 DD820 pKa = 3.93 ISFSYY825 pKa = 9.92 MGVNAISGITSTAAVTNDD843 pKa = 3.36 TSPVINGALTTALDD857 pKa = 3.46 SGDD860 pKa = 3.35 IVNVYY865 pKa = 10.44 RR866 pKa = 11.84 DD867 pKa = 3.69 GVKK870 pKa = 10.48 VGTATVSGNAWTFADD885 pKa = 4.16 SGLIDD890 pKa = 3.95 GTTYY894 pKa = 10.51 IYY896 pKa = 8.54 TAKK899 pKa = 10.63 VADD902 pKa = 3.74 PAGNEE907 pKa = 4.46 TVTGTAFTMTIDD919 pKa = 3.52 TTAPTATASVTGYY932 pKa = 10.33 ADD934 pKa = 3.24 NVGGVTSTASTAPTTDD950 pKa = 3.19 DD951 pKa = 3.55 TSPVINGTLSAPLAVGEE968 pKa = 4.3 VVNVYY973 pKa = 10.46 RR974 pKa = 11.84 GTTLIGTATVNGLTWTYY991 pKa = 11.35 ADD993 pKa = 4.03 SGLANSTTYY1002 pKa = 10.67 AYY1004 pKa = 9.62 KK1005 pKa = 10.82 ARR1007 pKa = 11.84 VADD1010 pKa = 3.62 AAGNISATSVALNITIDD1027 pKa = 3.54 TTTPGIDD1034 pKa = 3.37 VTPPTIIGFSSATVDD1049 pKa = 3.21 GTYY1052 pKa = 11.07 GDD1054 pKa = 4.05 GSIINITATASEE1066 pKa = 4.37 PVAAGSSIDD1075 pKa = 3.62 ILLDD1079 pKa = 3.29 TGDD1082 pKa = 3.61 IVHH1085 pKa = 6.85 LVAAADD1091 pKa = 3.98 GTTMIGDD1098 pKa = 4.27 YY1099 pKa = 10.18 IVSPGDD1105 pKa = 3.06 TSTDD1109 pKa = 3.4 LKK1111 pKa = 10.76 VLSYY1115 pKa = 10.19 TAGTVAAPSVHH1126 pKa = 7.0 DD1127 pKa = 3.88 LAGNAMTSTAMPVASANIDD1146 pKa = 3.27 AAQAIVVDD1154 pKa = 4.71 SPIQAPATLALMGDD1168 pKa = 3.91 RR1169 pKa = 11.84 LDD1171 pKa = 5.45 DD1172 pKa = 3.89 HH1173 pKa = 7.2 LGWDD1177 pKa = 3.66 VANIGDD1183 pKa = 4.31 FNNDD1187 pKa = 3.78 GISDD1191 pKa = 4.01 FAISAPQWDD1200 pKa = 4.03 INPNLFGAYY1209 pKa = 9.49 DD1210 pKa = 3.63 AGAVYY1215 pKa = 10.08 IVYY1218 pKa = 10.36 GNQTGLPNVGDD1229 pKa = 3.94 MQNLTVAQGFRR1240 pKa = 11.84 LTGHH1244 pKa = 7.07 DD1245 pKa = 4.55 ASNLLYY1251 pKa = 10.86 GGDD1254 pKa = 4.1 AIGEE1258 pKa = 4.32 IINPLGDD1265 pKa = 3.49 INGDD1269 pKa = 3.54 GYY1271 pKa = 11.89 ADD1273 pKa = 3.97 LGIGAKK1279 pKa = 8.55 NTDD1282 pKa = 3.08 TAYY1285 pKa = 11.33 VLLGGNAATFSATTYY1300 pKa = 10.43 DD1301 pKa = 3.46 IEE1303 pKa = 4.65 SVNGNIFSIAGGGAFGYY1320 pKa = 9.67 SVSGADD1326 pKa = 3.76 VNNDD1330 pKa = 3.23 GYY1332 pKa = 11.71 SDD1334 pKa = 3.73 ILIGAPLVDD1343 pKa = 4.84 NSILDD1348 pKa = 3.6 WDD1350 pKa = 4.04 RR1351 pKa = 11.84 GRR1353 pKa = 11.84 AYY1355 pKa = 9.9 IMYY1358 pKa = 8.96 GHH1360 pKa = 7.54 AGAATDD1366 pKa = 4.11 VNFTDD1371 pKa = 3.68 VTLTSNTFTNQTLSSVLSASNDD1393 pKa = 3.27 LPLFADD1399 pKa = 4.29 ANDD1402 pKa = 3.66 ASIANPYY1409 pKa = 10.01 RR1410 pKa = 11.84 LGDD1413 pKa = 4.04 SISNVGDD1420 pKa = 3.54 VNGDD1424 pKa = 4.01 GINDD1428 pKa = 3.88 FVVTAPTSDD1437 pKa = 3.0 NAMSGTGNAGSAWLVYY1453 pKa = 9.2 GQQGKK1458 pKa = 7.44 YY1459 pKa = 7.26 TFNVDD1464 pKa = 4.53 LSNLTLAQGVQINAYY1479 pKa = 9.88 GGEE1482 pKa = 4.09 LLGGEE1487 pKa = 4.37 GSGHH1491 pKa = 6.55 NLTTALGDD1499 pKa = 3.58 INGDD1503 pKa = 3.55 GVGDD1507 pKa = 3.84 FAISAPSSAATGSDD1521 pKa = 3.18 GQVWVIYY1528 pKa = 9.82 GRR1530 pKa = 11.84 NGGLTHH1536 pKa = 7.99 DD1537 pKa = 4.13 INLNVNAPFLSTSLGYY1553 pKa = 10.63 DD1554 pKa = 3.03 SGVNPFTAADD1564 pKa = 3.84 GFLLRR1569 pKa = 11.84 SEE1571 pKa = 5.45 LEE1573 pKa = 4.15 FLTSSNLGVSIRR1585 pKa = 11.84 GGQDD1589 pKa = 2.64 INGDD1593 pKa = 3.96 GIDD1596 pKa = 4.43 DD1597 pKa = 4.15 FVVSDD1602 pKa = 3.72 PTQNQTWEE1610 pKa = 4.03 GDD1612 pKa = 3.26 AGSVYY1617 pKa = 10.51 VVYY1620 pKa = 10.23 GQDD1623 pKa = 3.26 GGFGSNDD1630 pKa = 2.48 ITLPNLLANSQQAVEE1645 pKa = 4.38 IKK1647 pKa = 10.56 GLTGGDD1653 pKa = 3.46 TLGSGVALGDD1663 pKa = 3.59 WNGDD1667 pKa = 3.51 GLSEE1671 pKa = 4.63 IAIGQANGDD1680 pKa = 3.83 SSNGFLVNDD1689 pKa = 5.12 DD1690 pKa = 3.46 IGGAFIMPSISQLTQAVIATANTITAINGSPDD1722 pKa = 3.5 RR1723 pKa = 11.84 VSGGAGNDD1731 pKa = 3.94 TINSVSTKK1739 pKa = 10.17 DD1740 pKa = 3.39 VAYY1743 pKa = 9.57 GGSGADD1749 pKa = 3.53 VINIISNDD1757 pKa = 3.34 FTRR1760 pKa = 11.84 VDD1762 pKa = 3.42 GGLGIDD1768 pKa = 3.89 TLTVASPSAMVLDD1781 pKa = 4.69 LAALGSKK1788 pKa = 8.63 VQNFEE1793 pKa = 4.11 KK1794 pKa = 10.56 FDD1796 pKa = 3.95 LGVADD1801 pKa = 5.05 GVGHH1805 pKa = 6.88 TINLRR1810 pKa = 11.84 LSDD1813 pKa = 4.08 VLNEE1817 pKa = 4.09 TTNTVLGHH1825 pKa = 5.28 MEE1827 pKa = 4.11 VTGGAVDD1834 pKa = 3.74 TVNLNGTGNWITSSPVTASGITFDD1858 pKa = 4.21 IYY1860 pKa = 11.27 HH1861 pKa = 6.62 NNLLDD1866 pKa = 3.94 AANTLGDD1873 pKa = 3.25 VWIQQGVNVAYY1884 pKa = 9.34 TPDD1887 pKa = 3.26 TSAPLVLNFSSTTADD1902 pKa = 2.9 GSYY1905 pKa = 10.84 AAGSSINITATMTEE1919 pKa = 4.36 SVKK1922 pKa = 10.67 AGSSIDD1928 pKa = 3.49 VTLNTGDD1935 pKa = 5.09 LIHH1938 pKa = 6.89 LVAAADD1944 pKa = 3.81 GTALVGTYY1952 pKa = 8.54 TVGLGDD1958 pKa = 3.77 AAADD1962 pKa = 3.78 LKK1964 pKa = 11.08 VISYY1968 pKa = 7.72 TAGTFAAPAVHH1979 pKa = 7.18 DD1980 pKa = 4.05 LAGNAMTNMAMPVANANIDD1999 pKa = 3.48 AVQAIVVAAPIAEE2012 pKa = 4.26 PAVDD2016 pKa = 3.92 TLKK2019 pKa = 11.12 GDD2021 pKa = 3.49 NGGDD2025 pKa = 3.29 NLGRR2029 pKa = 11.84 DD2030 pKa = 3.79 VSNVGDD2036 pKa = 3.83 FNGDD2040 pKa = 4.03 GIADD2044 pKa = 4.43 FAVSAPNYY2052 pKa = 8.96 DD2053 pKa = 3.3 ATSGFFGGNNEE2064 pKa = 3.78 GGVYY2068 pKa = 9.69 VVYY2071 pKa = 8.87 GTTAGIPVIGDD2082 pKa = 3.35 IGSMAITQGLKK2093 pKa = 8.58 ITGASPGDD2101 pKa = 3.85 VIGKK2105 pKa = 8.02 MINAVGDD2112 pKa = 3.69 VNGDD2116 pKa = 3.39 GFADD2120 pKa = 3.99 LVIGATAVNKK2130 pKa = 10.36 AYY2132 pKa = 10.64 VVLGSNIAYY2141 pKa = 7.66 ITGSVSLGAVLGTTTSNNIFAITASSNIEE2170 pKa = 3.48 AGYY2173 pKa = 9.98 SVSGVDD2179 pKa = 3.91 VNNDD2183 pKa = 3.26 GYY2185 pKa = 11.73 SDD2187 pKa = 3.67 ILFGAPVAGSGAGAGYY2203 pKa = 11.1 VMYY2206 pKa = 10.34 GHH2208 pKa = 7.3 GGAITNVTFPDD2219 pKa = 3.26 ITLSTISGEE2228 pKa = 4.31 ANTFANGTIGTKK2240 pKa = 10.44 LLPSHH2245 pKa = 7.19 DD2246 pKa = 4.7 LNLLGLSNDD2255 pKa = 3.44 QANSGRR2261 pKa = 11.84 LGEE2264 pKa = 4.2 ATSNVGDD2271 pKa = 3.87 VNGDD2275 pKa = 3.79 GIDD2278 pKa = 3.94 DD2279 pKa = 5.22 FIFTASTSTNALSPLLAGGSAWLVYY2304 pKa = 9.35 GQQGGFGASLDD2315 pKa = 4.2 LNNLTLAQGVQLNGYY2330 pKa = 9.41 LGEE2333 pKa = 4.24 NLGGNAGRR2341 pKa = 11.84 NVTTALGDD2349 pKa = 3.55 INGDD2353 pKa = 3.85 GIGDD2357 pKa = 3.88 FAIAAAQHH2365 pKa = 4.39 NHH2367 pKa = 3.68 VWVVYY2372 pKa = 9.35 GVNGGHH2378 pKa = 7.15 ANNINLDD2385 pKa = 4.02 TNTLTAADD2393 pKa = 4.01 GFVLNSEE2400 pKa = 4.3 FDD2402 pKa = 3.38 LGGSQLGASIRR2413 pKa = 11.84 GGQDD2417 pKa = 2.56 INGDD2421 pKa = 3.75 GLDD2424 pKa = 4.25 DD2425 pKa = 4.21 FVVSAPNQDD2434 pKa = 3.32 LGLAIDD2440 pKa = 4.48 AGAAYY2445 pKa = 10.16 VIYY2448 pKa = 9.6 GQKK2451 pKa = 10.7 GGFGSNSVDD2460 pKa = 3.77 LSALLTSSQQAIKK2473 pKa = 10.46 ISGQGRR2479 pKa = 11.84 LGSGVAVGDD2488 pKa = 3.75 WNGDD2492 pKa = 3.15 GVADD2496 pKa = 3.66 IGIGRR2501 pKa = 11.84 EE2502 pKa = 3.8 SEE2504 pKa = 4.42 YY2505 pKa = 10.91 FFGDD2509 pKa = 3.58 GSAYY2513 pKa = 10.27 VLPGTSQLTQTGDD2526 pKa = 2.95 SGNNIIAAVTKK2537 pKa = 10.4 SEE2539 pKa = 3.81 EE2540 pKa = 4.44 RR2541 pKa = 11.84 ISGAQGNDD2549 pKa = 3.27 TINNVSTLDD2558 pKa = 3.48 VAYY2561 pKa = 10.34 GGAGDD2566 pKa = 4.72 DD2567 pKa = 4.89 LISIASNDD2575 pKa = 3.43 FTRR2578 pKa = 11.84 VDD2580 pKa = 3.32 GGLGVDD2586 pKa = 3.74 TLSLAGAPAMTLNFSSLGSKK2606 pKa = 9.28 VQGFEE2611 pKa = 4.45 KK2612 pKa = 10.45 FDD2614 pKa = 3.59 LGAADD2619 pKa = 4.66 GVGHH2623 pKa = 5.43 TLKK2626 pKa = 10.78 LRR2628 pKa = 11.84 LSDD2631 pKa = 4.26 VLNEE2635 pKa = 4.31 TNASTLGHH2643 pKa = 5.27 MQVTGGATDD2652 pKa = 3.59 TVNLSGTWATSSTATASGVTFDD2674 pKa = 4.37 VYY2676 pKa = 11.2 HH2677 pKa = 6.75 NSALAAGNTLGDD2689 pKa = 3.25 VWVQQGVVVVSTNGLDD2705 pKa = 3.41 TTAPLAPVATVAANGATVTGTAEE2728 pKa = 4.15 AGSTVTIKK2736 pKa = 10.77 NAANTILGTTVAALDD2751 pKa = 3.89 GSYY2754 pKa = 10.78 SVPLSPALTNGEE2766 pKa = 4.49 ALTATATDD2774 pKa = 3.39 AANNVSPVASAMAPDD2789 pKa = 4.14 TTAPTASVTTATIDD2803 pKa = 3.52 TVTNAVVQSTEE2814 pKa = 3.43 VGTAYY2819 pKa = 10.49 LVKK2822 pKa = 10.36 DD2823 pKa = 3.7 TVTVTNLASITSAADD2838 pKa = 3.46 NVWNQVPISTANSNTNLVATGLLDD2862 pKa = 3.44 GTYY2865 pKa = 10.64 KK2866 pKa = 10.87 VYY2868 pKa = 10.08 TVDD2871 pKa = 3.56 AAGNLSAASTNSITVADD2888 pKa = 4.23 TALHH2892 pKa = 6.66 LGPVAGVNLNLIAPVTMADD2911 pKa = 3.11 GKK2913 pKa = 11.03 VYY2915 pKa = 10.92 YY2916 pKa = 10.45 HH2917 pKa = 7.32 LDD2919 pKa = 3.34 QSGDD2923 pKa = 3.69 GVFSGSDD2930 pKa = 3.34 HH2931 pKa = 7.03 VDD2933 pKa = 2.98 HH2934 pKa = 7.69 DD2935 pKa = 5.28 LLDD2938 pKa = 4.08 ALLNGGADD2946 pKa = 3.96 TINTQLSGAVAGVDD2960 pKa = 3.61 DD2961 pKa = 4.69 EE2962 pKa = 4.41 RR2963 pKa = 11.84 TIIVGGYY2970 pKa = 6.86 TLVLPTVAEE2979 pKa = 4.11 LTALYY2984 pKa = 10.25 NDD2986 pKa = 4.92 PAPNPPVGWDD2996 pKa = 3.67 NINHH3000 pKa = 5.55 YY3001 pKa = 9.58 WSATLYY3007 pKa = 10.96 SPTAHH3012 pKa = 6.19 YY3013 pKa = 11.09 NEE3015 pKa = 5.31 LLTTGQVNGYY3025 pKa = 10.47 ADD3027 pKa = 3.75 GNGFSVTFQVIANPVVANPLTFTSMSIDD3055 pKa = 3.57 LATASDD3061 pKa = 3.95 NGVSTTDD3068 pKa = 3.52 NITSNTAPTINGTVTGLSATVAAAATAGTVTANLFDD3104 pKa = 5.03 DD3105 pKa = 4.87 KK3106 pKa = 11.39 NDD3108 pKa = 3.46 NGLYY3112 pKa = 10.48 DD3113 pKa = 4.68 AGDD3116 pKa = 3.48 SLLAVNIPLSVTGTNATFTAQPTLLGGTYY3145 pKa = 7.43 NTKK3148 pKa = 10.69 AILIDD3153 pKa = 3.5 QANNASVAGLLDD3165 pKa = 3.5 NSGTAQVVVDD3175 pKa = 3.78 WTAAPSVVAGNQANDD3190 pKa = 3.57 GLGYY3194 pKa = 11.48 AMANAGDD3201 pKa = 4.07 FNGDD3205 pKa = 3.1 GYY3207 pKa = 11.82 ADD3209 pKa = 5.48 FILTAPHH3216 pKa = 6.54 DD3217 pKa = 3.89 QSSSFDD3223 pKa = 3.07 IGVRR3227 pKa = 11.84 KK3228 pKa = 9.68 SDD3230 pKa = 3.28 MYY3232 pKa = 10.6 IVYY3235 pKa = 10.42 GSATGLTNVGDD3246 pKa = 4.28 IDD3248 pKa = 4.29 TMTAAQGIHH3257 pKa = 5.56 ITSTAIVGADD3267 pKa = 2.98 IALQGLVTSGIGDD3280 pKa = 4.13 LNGDD3284 pKa = 4.16 GYY3286 pKa = 11.8 ADD3288 pKa = 3.55 VAIGSHH3294 pKa = 6.76 RR3295 pKa = 11.84 DD3296 pKa = 3.09 DD3297 pKa = 3.23 SLYY3300 pKa = 11.18 VVFGRR3305 pKa = 11.84 GGNSTATFDD3314 pKa = 4.05 LRR3316 pKa = 11.84 SIEE3319 pKa = 4.24 TGATTNGFHH3328 pKa = 6.75 IANWATGAWMATGLGGGDD3346 pKa = 3.58 VNGDD3350 pKa = 3.51 GYY3352 pKa = 11.31 GDD3354 pKa = 4.16 LMFGSSDD3361 pKa = 3.25 GLGNGLAYY3369 pKa = 9.48 TLYY3372 pKa = 10.35 GHH3374 pKa = 7.46 AGASGDD3380 pKa = 3.89 ASWSNLYY3387 pKa = 8.67 ATATEE3392 pKa = 4.18 GLHH3395 pKa = 6.23 VKK3397 pKa = 8.87 GTLGVYY3403 pKa = 8.41 GAPLDD3408 pKa = 4.07 AGAYY3412 pKa = 5.17 TTLSTTYY3419 pKa = 10.66 DD3420 pKa = 3.6 PTGQAHH3426 pKa = 7.27 DD3427 pKa = 4.29 FDD3429 pKa = 6.48 SDD3431 pKa = 3.35 LGDD3434 pKa = 3.53 RR3435 pKa = 11.84 VTVISDD3441 pKa = 3.57 VNGDD3445 pKa = 3.74 GYY3447 pKa = 11.67 NDD3449 pKa = 4.33 YY3450 pKa = 10.95 IVTAPRR3456 pKa = 11.84 ADD3458 pKa = 3.42 TTTSFDD3464 pKa = 3.24 AGSAYY3469 pKa = 10.97 LLFGSASGLGSGFDD3483 pKa = 4.51 LGNLSPSQGVRR3494 pKa = 11.84 LNGTEE3499 pKa = 3.71 NYY3501 pKa = 8.94 EE3502 pKa = 3.99 YY3503 pKa = 11.04 LGGSTLLAGGIGDD3516 pKa = 4.83 HH3517 pKa = 7.31 GDD3519 pKa = 3.71 ALWGQGASVSNIGDD3533 pKa = 3.4 INADD3537 pKa = 3.3 GVADD3541 pKa = 4.18 FAIGSPTWGDD3551 pKa = 3.42 QVADD3555 pKa = 4.05 GQAAGRR3561 pKa = 11.84 VYY3563 pKa = 10.98 VLYY3566 pKa = 10.78 GKK3568 pKa = 10.78 GIGANWADD3576 pKa = 3.16 VDD3578 pKa = 4.41 LANLNGSNGFIINSTALGSTSSIIANSQDD3607 pKa = 3.16 AQLGFSISNGGDD3619 pKa = 3.36 VNGDD3623 pKa = 3.4 GVDD3626 pKa = 3.93 DD3627 pKa = 5.26 FIISAPGADD3636 pKa = 3.69 NGATTDD3642 pKa = 2.98 TGAAYY3647 pKa = 10.16 LVYY3650 pKa = 9.68 GQAGGTSFTAITNLDD3665 pKa = 3.78 SLVSSGKK3672 pKa = 9.68 AIKK3675 pKa = 10.6 YY3676 pKa = 10.3 SGINTKK3682 pKa = 10.71 DD3683 pKa = 3.33 FAGTGLATGDD3693 pKa = 3.25 WDD3695 pKa = 3.89 GDD3697 pKa = 4.0 GIADD3701 pKa = 3.33 VTYY3704 pKa = 10.77 SVWEE3708 pKa = 4.23 SDD3710 pKa = 3.09 TATIDD3715 pKa = 3.14 NGGSYY3720 pKa = 10.2 VVYY3723 pKa = 10.62 KK3724 pKa = 10.94 GGLSNLTQSFTTGNDD3739 pKa = 2.97 SLTAGTNSGGATIVNGVDD3757 pKa = 3.72 RR3758 pKa = 11.84 ISGGLGNDD3766 pKa = 3.85 SITGIGTDD3774 pKa = 3.62 TTGTTATTTLHH3785 pKa = 6.37 DD3786 pKa = 3.65 VAYY3789 pKa = 9.92 GGQGNDD3795 pKa = 3.73 HH3796 pKa = 6.88 IALAGTNFTRR3806 pKa = 11.84 VDD3808 pKa = 3.34 GGLGIDD3814 pKa = 3.62 TLGLTGATALSLDD3827 pKa = 3.55 LTPYY3831 pKa = 7.41 NTRR3834 pKa = 11.84 VKK3836 pKa = 10.72 GFEE3839 pKa = 4.06 KK3840 pKa = 10.5 FDD3842 pKa = 3.95 LGASDD3847 pKa = 4.68 SVGHH3851 pKa = 5.34 TLSMRR3856 pKa = 11.84 LADD3859 pKa = 4.03 VLNEE3863 pKa = 4.23 PGTNALGHH3871 pKa = 6.17 IEE3873 pKa = 4.3 VVGGAGDD3880 pKa = 3.73 KK3881 pKa = 10.9 VDD3883 pKa = 4.92 LSGEE3887 pKa = 4.45 GSWTTATTQAASGVTYY3903 pKa = 10.81 NVYY3906 pKa = 10.67 HH3907 pKa = 6.84 NSALDD3912 pKa = 3.55 AANTKK3917 pKa = 10.31 GDD3919 pKa = 3.12 VWVQQGVTVNGTNTSASDD3937 pKa = 3.67 NNVPLAGTAVLPVTLSVSSTSSSTQINDD3965 pKa = 3.42 GLGFAMSDD3973 pKa = 2.93 AGDD3976 pKa = 3.9 FNGDD3980 pKa = 3.12 GYY3982 pKa = 9.78 TDD3984 pKa = 4.38 YY3985 pKa = 10.96 IVSAPHH3991 pKa = 5.43 NQIGGLAVAAGNMYY4005 pKa = 10.06 IVYY4008 pKa = 10.0 GSQTGLPTLSNIDD4021 pKa = 3.59 SMTASQGIKK4030 pKa = 9.81 LASNSLYY4037 pKa = 10.8 ADD4039 pKa = 5.28 GYY4041 pKa = 9.45 QGMVVTSIGDD4051 pKa = 3.61 LNGDD4055 pKa = 3.97 GFADD4059 pKa = 3.69 VAIGSILNDD4068 pKa = 2.97 SLYY4071 pKa = 11.22 VVFGRR4076 pKa = 11.84 GGNTTSALQLTDD4088 pKa = 3.84 LDD4090 pKa = 4.2 NVATADD4096 pKa = 3.58 GFRR4099 pKa = 11.84 IRR4101 pKa = 11.84 NWDD4104 pKa = 3.06 TGAWFASGLSGGDD4117 pKa = 3.53 VNNDD4121 pKa = 3.23 GYY4123 pKa = 11.4 SDD4125 pKa = 4.21 LMFGSSDD4132 pKa = 3.21 GLGNGMYY4139 pKa = 7.97 FTLYY4143 pKa = 10.34 GHH4145 pKa = 6.92 TGAAGAANWGNILAEE4160 pKa = 4.36 TTSLNTSTNSQLPGDD4175 pKa = 4.1 EE4176 pKa = 4.87 LNRR4179 pKa = 11.84 SAYY4182 pKa = 8.75 TAVKK4186 pKa = 7.15 TTYY4189 pKa = 10.11 ISQTQATVHH4198 pKa = 6.83 DD4199 pKa = 5.09 LDD4201 pKa = 5.14 SDD4203 pKa = 3.63 LGDD4206 pKa = 3.95 RR4207 pKa = 11.84 IKK4209 pKa = 10.72 IVGDD4213 pKa = 3.4 VNGDD4217 pKa = 3.56 GFNDD4221 pKa = 3.74 YY4222 pKa = 11.07 VVTAPRR4228 pKa = 11.84 AEE4230 pKa = 4.13 ATASTFDD4237 pKa = 3.12 AGRR4240 pKa = 11.84 AYY4242 pKa = 11.32 LMFGTEE4248 pKa = 3.88 AGLGKK4253 pKa = 10.8 GFDD4256 pKa = 4.61 LNDD4259 pKa = 3.99 LSPTQGIRR4267 pKa = 11.84 INGTEE4272 pKa = 3.94 NYY4274 pKa = 8.65 EE4275 pKa = 4.08 DD4276 pKa = 4.26 LGGSTLFAGGNDD4288 pKa = 4.04 DD4289 pKa = 5.57 NADD4292 pKa = 3.25 AYY4294 pKa = 11.24 YY4295 pKa = 11.54 GMGSSVNSIGDD4306 pKa = 3.49 INGDD4310 pKa = 3.95 GITDD4314 pKa = 3.99 FAIGSPEE4321 pKa = 3.92 WGDD4324 pKa = 3.55 QVADD4328 pKa = 3.75 AQSTGRR4334 pKa = 11.84 TYY4336 pKa = 10.55 IVYY4339 pKa = 10.19 GKK4341 pKa = 9.78 GAGMDD4346 pKa = 3.1 WSDD4349 pKa = 4.12 INLASLGSQGFIMQGSITDD4368 pKa = 3.25 VSNGFNFNTTHH4379 pKa = 7.01 HH4380 pKa = 6.95 DD4381 pKa = 3.29 QLGFSVSNGGDD4392 pKa = 3.46 VNGDD4396 pKa = 3.77 GIDD4399 pKa = 4.06 DD4400 pKa = 4.12 FLIGAPGEE4408 pKa = 4.18 TAGALKK4414 pKa = 10.38 DD4415 pKa = 3.95 YY4416 pKa = 11.17 AGAAYY4421 pKa = 10.12 LVFGQAGGTGFLSTTGLDD4439 pKa = 3.6 VLVTANKK4446 pKa = 9.96 AIRR4449 pKa = 11.84 FAGVNADD4456 pKa = 4.43 DD4457 pKa = 3.91 YY4458 pKa = 10.35 TGTGVTIGDD4467 pKa = 3.71 WNGDD4471 pKa = 3.63 GIADD4475 pKa = 3.53 VSYY4478 pKa = 8.41 GTWEE4482 pKa = 3.96 NGATNTGSYY4491 pKa = 8.17 QVYY4494 pKa = 10.73 NGATSNLTQTFTLGNDD4510 pKa = 3.1 TLAAGTHH4517 pKa = 5.92 NGSGPVLVAGVDD4529 pKa = 3.99 RR4530 pKa = 11.84 ISGGQGNDD4538 pKa = 2.99 TLTGIGTDD4546 pKa = 3.39 TTGTMDD4552 pKa = 3.37 TTSLHH4557 pKa = 6.02 DD4558 pKa = 3.52 VAYY4561 pKa = 10.51 GGAGDD4566 pKa = 5.46 DD4567 pKa = 4.27 NISLAGMSFTRR4578 pKa = 11.84 VDD4580 pKa = 3.23 GGLGIDD4586 pKa = 3.75 TLTLAGTSSLSLNIAEE4602 pKa = 4.26 YY4603 pKa = 10.41 GNRR4606 pKa = 11.84 VKK4608 pKa = 10.95 GFEE4611 pKa = 4.09 KK4612 pKa = 10.53 FDD4614 pKa = 4.05 LGSSDD4619 pKa = 4.92 GIGHH4623 pKa = 6.44 NLSLRR4628 pKa = 11.84 LSDD4631 pKa = 4.78 VLNEE4635 pKa = 4.32 TNSSVLGHH4643 pKa = 5.45 MQVTGGAGDD4652 pKa = 3.89 SVQLNGSGTWTTATTATATGITFDD4676 pKa = 4.01 VYY4678 pKa = 11.33 HH4679 pKa = 5.91 NTNLDD4684 pKa = 3.46 AANKK4688 pKa = 10.36 LGDD4691 pKa = 3.3 VWIQQGMAVLL4701 pKa = 4.19
Molecular weight: 470.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.579
IPC_protein 3.668
Toseland 3.401
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.656
Rodwell 3.478
Grimsley 3.312
Solomon 3.681
Lehninger 3.643
Nozaki 3.783
DTASelect 4.126
Thurlkill 3.465
EMBOSS 3.656
Sillero 3.795
Patrickios 0.858
IPC_peptide 3.668
IPC2_peptide 3.757
IPC2.peptide.svr19 3.72
Protein with the highest isoelectric point:
>tr|A0A1R4H709|A0A1R4H709_9GAMM XRE family transcriptional regulator OS=Crenothrix polyspora OX=360316 GN=CRENPOLYSF1_230043 PE=4 SV=1
MM1 pKa = 6.24 VTIRR5 pKa = 11.84 LSRR8 pKa = 11.84 GGAKK12 pKa = 9.54 NRR14 pKa = 11.84 PFYY17 pKa = 10.82 HH18 pKa = 6.12 VVVTDD23 pKa = 3.58 SRR25 pKa = 11.84 SSRR28 pKa = 11.84 DD29 pKa = 2.72 GRR31 pKa = 11.84 YY32 pKa = 8.84 IEE34 pKa = 4.5 RR35 pKa = 11.84 VGFFNPLARR44 pKa = 11.84 GNEE47 pKa = 3.62 EE48 pKa = 4.1 RR49 pKa = 11.84 LRR51 pKa = 11.84 LDD53 pKa = 3.43 SARR56 pKa = 11.84 VDD58 pKa = 3.2 YY59 pKa = 10.34 WKK61 pKa = 9.8 ATGAQPSEE69 pKa = 4.13 RR70 pKa = 11.84 VAKK73 pKa = 10.38 LIKK76 pKa = 10.33 DD77 pKa = 3.74 ALKK80 pKa = 10.64 AAAA83 pKa = 4.8
Molecular weight: 9.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.765
IPC_protein 10.716
Toseland 10.804
ProMoST 10.599
Dawson 10.891
Bjellqvist 10.643
Wikipedia 11.14
Rodwell 11.023
Grimsley 10.935
Solomon 11.052
Lehninger 11.023
Nozaki 10.774
DTASelect 10.643
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.833
Patrickios 10.789
IPC_peptide 11.067
IPC2_peptide 9.531
IPC2.peptide.svr19 8.606
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3943
0
3943
1122853
20
4701
284.8
31.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.095 ± 0.044
1.118 ± 0.017
5.456 ± 0.033
5.137 ± 0.042
4.091 ± 0.031
6.758 ± 0.052
2.425 ± 0.02
6.59 ± 0.034
5.488 ± 0.047
10.583 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.363 ± 0.023
4.548 ± 0.038
4.132 ± 0.029
4.429 ± 0.039
4.496 ± 0.029
6.241 ± 0.035
5.868 ± 0.048
6.758 ± 0.034
1.295 ± 0.017
3.129 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here