Beihai sphaeromadae virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KHV5|A0A1L3KHV5_9VIRU Putative capsid protein OS=Beihai sphaeromadae virus 4 OX=1922710 PE=4 SV=1
MM1 pKa = 7.68PSRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84IRR8 pKa = 11.84QPIRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84MPKK17 pKa = 10.11RR18 pKa = 11.84IRR20 pKa = 11.84QPKK23 pKa = 9.08PVTSSQTLTSTYY35 pKa = 9.88DD36 pKa = 3.18IPIGLPSMTAFSTKK50 pKa = 10.14AVSLDD55 pKa = 3.47PSAHH59 pKa = 5.58EE60 pKa = 4.15NSRR63 pKa = 11.84ASAQMSIYY71 pKa = 10.86NKK73 pKa = 10.25YY74 pKa = 10.37KK75 pKa = 10.83LLACSLIYY83 pKa = 10.51SPSVGYY89 pKa = 10.49DD90 pKa = 3.04SSSGLLGVFHH100 pKa = 6.55STAEE104 pKa = 4.07EE105 pKa = 4.16EE106 pKa = 4.32IAPQTRR112 pKa = 11.84EE113 pKa = 4.0DD114 pKa = 3.64FQGRR118 pKa = 11.84IQNAGPSGCLIPVRR132 pKa = 11.84KK133 pKa = 9.84NKK135 pKa = 9.64VFTVPKK141 pKa = 10.21SVIRR145 pKa = 11.84NIAEE149 pKa = 4.67GYY151 pKa = 8.26TPGATQTPGRR161 pKa = 11.84IVIGSATSCDD171 pKa = 3.19IDD173 pKa = 4.45SPGLLTLRR181 pKa = 11.84STYY184 pKa = 10.15QFIGPTVPATPASPTKK200 pKa = 9.94IIKK203 pKa = 9.45RR204 pKa = 11.84APYY207 pKa = 8.69TRR209 pKa = 11.84RR210 pKa = 11.84LASFVWKK217 pKa = 7.95PTNPEE222 pKa = 3.36WFLISGLVDD231 pKa = 3.62SYY233 pKa = 11.89AATSDD238 pKa = 4.02AITDD242 pKa = 3.35VSIEE246 pKa = 3.82FSVYY250 pKa = 9.45NASGSSFGKK259 pKa = 10.59SIGQSYY265 pKa = 11.16NDD267 pKa = 3.37WQSFIEE273 pKa = 4.4SAFADD278 pKa = 3.92SQADD282 pKa = 3.45WEE284 pKa = 4.81TFTSSTLAAITFDD297 pKa = 3.98PPGSPFKK304 pKa = 10.37TAGILDD310 pKa = 3.73SQRR313 pKa = 11.84FVTTMPDD320 pKa = 2.99STTTTLVDD328 pKa = 3.65SVTTTLEE335 pKa = 3.92EE336 pKa = 4.58SVTTTLPLSQTTTLQDD352 pKa = 3.32SVTTTLQEE360 pKa = 4.44SVTTTLPEE368 pKa = 4.27SQTTTLEE375 pKa = 4.1DD376 pKa = 3.69SKK378 pKa = 9.02TTTLDD383 pKa = 3.33SSVTTTLPQSQTTTLQGSIVKK404 pKa = 8.58TEE406 pKa = 4.33PGSVTTTDD414 pKa = 3.16SDD416 pKa = 4.08STTKK420 pKa = 9.99TLSGSVTTTDD430 pKa = 3.71EE431 pKa = 3.99NSKK434 pKa = 7.89TTTQIEE440 pKa = 4.4SRR442 pKa = 11.84VYY444 pKa = 10.02IALPNQGVVSSNNPLPVDD462 pKa = 4.04DD463 pKa = 4.91GGGGFFMNLATGVITAFVLEE483 pKa = 4.56KK484 pKa = 10.28GRR486 pKa = 11.84KK487 pKa = 8.12YY488 pKa = 11.03GPDD491 pKa = 3.13SKK493 pKa = 9.86EE494 pKa = 4.01TPEE497 pKa = 4.53GYY499 pKa = 10.3VRR501 pKa = 11.84LKK503 pKa = 9.53GTYY506 pKa = 8.91PAGGDD511 pKa = 3.44VDD513 pKa = 4.19GVSYY517 pKa = 10.92PDD519 pKa = 3.4VNAPSSTTSVDD530 pKa = 3.38YY531 pKa = 11.09KK532 pKa = 10.82QFTSFDD538 pKa = 3.81VLIKK542 pKa = 9.79PQNFEE547 pKa = 3.79GTGWHH552 pKa = 5.85QKK554 pKa = 10.1LDD556 pKa = 3.91SVICRR561 pKa = 11.84HH562 pKa = 5.5YY563 pKa = 10.7LGPAYY568 pKa = 10.29YY569 pKa = 10.91GNLQSRR575 pKa = 11.84VAIDD579 pKa = 3.4VTVAPILFEE588 pKa = 4.54EE589 pKa = 5.17ADD591 pKa = 3.72TGNKK595 pKa = 9.38ILGDD599 pKa = 3.62PCRR602 pKa = 11.84YY603 pKa = 9.13RR604 pKa = 11.84ALYY607 pKa = 10.31YY608 pKa = 10.59KK609 pKa = 9.45DD610 pKa = 3.69TNDD613 pKa = 2.94FRR615 pKa = 11.84VIRR618 pKa = 11.84SSTEE622 pKa = 3.79VEE624 pKa = 3.69AKK626 pKa = 10.66LYY628 pKa = 7.68DD629 pKa = 3.66TQSAIGFRR637 pKa = 11.84VIPVMSDD644 pKa = 3.07YY645 pKa = 10.86PIYY648 pKa = 10.51EE649 pKa = 4.79DD650 pKa = 5.12EE651 pKa = 4.45SKK653 pKa = 8.84TWFAIYY659 pKa = 9.87GHH661 pKa = 6.24NVDD664 pKa = 4.14TNLL667 pKa = 4.15

Molecular weight:
72.51 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KHV5|A0A1L3KHV5_9VIRU Putative capsid protein OS=Beihai sphaeromadae virus 4 OX=1922710 PE=4 SV=1
MM1 pKa = 7.76ASFSNPVNASEE12 pKa = 4.43KK13 pKa = 10.62LSGASFLAVATRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84QDD30 pKa = 3.05LSFLEE35 pKa = 4.52GQLDD39 pKa = 3.38KK40 pKa = 10.59CTRR43 pKa = 11.84LSPQFHH49 pKa = 6.55LPIRR53 pKa = 11.84DD54 pKa = 3.24IPEE57 pKa = 3.87IKK59 pKa = 9.44STGKK63 pKa = 11.03GDD65 pKa = 3.41MQRR68 pKa = 11.84AKK70 pKa = 9.41TQTFDD75 pKa = 3.82VPDD78 pKa = 4.05NFEE81 pKa = 4.59KK82 pKa = 10.88LPPIPNSKK90 pKa = 8.61LTRR93 pKa = 11.84NGEE96 pKa = 4.3LIQKK100 pKa = 9.97GNLGKK105 pKa = 10.37RR106 pKa = 11.84FYY108 pKa = 11.09NSQKK112 pKa = 9.23TFGGKK117 pKa = 8.8NVPDD121 pKa = 4.13NLRR124 pKa = 11.84QTIRR128 pKa = 11.84HH129 pKa = 5.59ALATRR134 pKa = 11.84IEE136 pKa = 4.38INGPAVEE143 pKa = 4.39SCVIADD149 pKa = 3.71EE150 pKa = 4.78LMATGLEE157 pKa = 4.2WEE159 pKa = 4.59YY160 pKa = 11.59SAGTTQGLVEE170 pKa = 4.02RR171 pKa = 11.84LSRR174 pKa = 11.84FGKK177 pKa = 10.05DD178 pKa = 2.92DD179 pKa = 3.29KK180 pKa = 10.83TVSFFRR186 pKa = 11.84QTDD189 pKa = 3.91EE190 pKa = 3.57IRR192 pKa = 11.84NFQSFLFQALDD203 pKa = 3.28TLLPLKK209 pKa = 9.03STPFEE214 pKa = 3.91TSFPSTSTEE223 pKa = 3.9VIEE226 pKa = 4.97GIRR229 pKa = 11.84VNPQSSAGPMYY240 pKa = 9.96GTTKK244 pKa = 10.39VQALDD249 pKa = 5.17DD250 pKa = 3.73IMLTMDD256 pKa = 5.59AIRR259 pKa = 11.84TQFSLGTLDD268 pKa = 3.53TFFAAHH274 pKa = 7.51PEE276 pKa = 4.04TLIADD281 pKa = 4.28CRR283 pKa = 11.84NKK285 pKa = 9.38TDD287 pKa = 3.18RR288 pKa = 11.84YY289 pKa = 9.91EE290 pKa = 3.98IAKK293 pKa = 10.14LPEE296 pKa = 3.66KK297 pKa = 9.66TRR299 pKa = 11.84PYY301 pKa = 10.33WSFNAHH307 pKa = 3.89WQFYY311 pKa = 8.49YY312 pKa = 10.41SWLMQRR318 pKa = 11.84FCDD321 pKa = 3.84NLYY324 pKa = 9.98VASDD328 pKa = 4.16PEE330 pKa = 4.95CDD332 pKa = 3.31VPTYY336 pKa = 10.94NYY338 pKa = 9.99YY339 pKa = 10.07GHH341 pKa = 6.53VWAKK345 pKa = 10.64GGAQRR350 pKa = 11.84IHH352 pKa = 6.17EE353 pKa = 4.83SIMNVAKK360 pKa = 10.01TGRR363 pKa = 11.84PFVGIYY369 pKa = 10.82GDD371 pKa = 4.08DD372 pKa = 3.63FLFAFPIKK380 pKa = 9.4TDD382 pKa = 3.25EE383 pKa = 4.27GTTIQVMAPDD393 pKa = 4.43ISQMDD398 pKa = 3.94STVSHH403 pKa = 5.93AVIRR407 pKa = 11.84GTCDD411 pKa = 3.23YY412 pKa = 10.54IYY414 pKa = 10.71KK415 pKa = 10.12QYY417 pKa = 11.08QEE419 pKa = 4.29AHH421 pKa = 6.44GDD423 pKa = 3.65DD424 pKa = 3.76PFVRR428 pKa = 11.84FLLTRR433 pKa = 11.84LAEE436 pKa = 4.57DD437 pKa = 3.34ASSPRR442 pKa = 11.84FLASAIATYY451 pKa = 10.49QNQRR455 pKa = 11.84PTGLATGVVGTTLFDD470 pKa = 3.64TVQAALLAFSVIEE483 pKa = 3.68EE484 pKa = 4.11WNRR487 pKa = 11.84TKK489 pKa = 10.84RR490 pKa = 11.84SPDD493 pKa = 3.23EE494 pKa = 3.99IFNKK498 pKa = 9.52VSKK501 pKa = 10.43KK502 pKa = 10.02FGMRR506 pKa = 11.84AKK508 pKa = 10.42SLEE511 pKa = 3.94LYY513 pKa = 9.45EE514 pKa = 4.55YY515 pKa = 10.94NGLPPAEE522 pKa = 4.08GTEE525 pKa = 4.08WTFLGMQLATYY536 pKa = 10.49SNSKK540 pKa = 7.91HH541 pKa = 5.35TNGVIPRR548 pKa = 11.84LPRR551 pKa = 11.84DD552 pKa = 3.56SLLKK556 pKa = 10.72SILNPRR562 pKa = 11.84INSYY566 pKa = 10.77LFSSKK571 pKa = 10.64LGTQRR576 pKa = 11.84YY577 pKa = 8.91LFDD580 pKa = 5.0ASRR583 pKa = 11.84GWYY586 pKa = 9.86LCALGDD592 pKa = 3.78RR593 pKa = 11.84LIWDD597 pKa = 3.73VLSSVIEE604 pKa = 4.27TVPTMAVGMVVQAGGGLGAPPEE626 pKa = 4.46CNGLFDD632 pKa = 4.79VNRR635 pKa = 11.84PFNWLSSFGVPTMDD649 pKa = 2.77WVADD653 pKa = 4.05LFLSPMDD660 pKa = 4.97PDD662 pKa = 3.78PAGWLSLLDD671 pKa = 3.86PPKK674 pKa = 10.78GRR676 pKa = 11.84LIVEE680 pKa = 4.29MQDD683 pKa = 3.26QVDD686 pKa = 4.11LSRR689 pKa = 11.84YY690 pKa = 6.89TIRR693 pKa = 11.84PTPNTAPHH701 pKa = 5.44SWEE704 pKa = 3.81TRR706 pKa = 11.84EE707 pKa = 4.99KK708 pKa = 10.13VTDD711 pKa = 3.62TNLEE715 pKa = 4.19SLVSDD720 pKa = 3.88NPSGLGPQVQRR731 pKa = 11.84PKK733 pKa = 10.51AQHH736 pKa = 6.23LPPVKK741 pKa = 10.41APPFKK746 pKa = 10.41IRR748 pKa = 11.84LSEE751 pKa = 4.1HH752 pKa = 6.27LSKK755 pKa = 11.18HH756 pKa = 5.96NITAFVPSEE765 pKa = 3.76LAAEE769 pKa = 4.53LGVSVTTILTEE780 pKa = 4.41LYY782 pKa = 10.45SKK784 pKa = 10.52EE785 pKa = 4.33RR786 pKa = 11.84EE787 pKa = 4.06TKK789 pKa = 9.72APEE792 pKa = 3.78FRR794 pKa = 11.84WYY796 pKa = 10.51SLGRR800 pKa = 11.84ILTKK804 pKa = 10.9DD805 pKa = 3.29PVDD808 pKa = 3.55TVQPSRR814 pKa = 11.84ILWNQYY820 pKa = 9.05SRR822 pKa = 11.84ILSEE826 pKa = 4.63LGYY829 pKa = 10.34SNPAIQPARR838 pKa = 11.84PKK840 pKa = 10.27AGPVPAPRR848 pKa = 11.84YY849 pKa = 10.25SKK851 pKa = 10.26MKK853 pKa = 10.78ANVPQDD859 pKa = 3.47FATSNSTTISDD870 pKa = 4.63CDD872 pKa = 3.4TSPAIRR878 pKa = 11.84VSGPDD883 pKa = 3.23QTPTSQTVPQYY894 pKa = 9.79TPKK897 pKa = 9.26PAQMMYY903 pKa = 10.64QRR905 pKa = 11.84IPAKK909 pKa = 9.83TGAVVCALEE918 pKa = 4.36EE919 pKa = 4.08NLVAPRR925 pKa = 11.84AEE927 pKa = 4.3GLSSGARR934 pKa = 11.84VPLADD939 pKa = 3.46QVNSGRR945 pKa = 11.84IVHH948 pKa = 6.36QYY950 pKa = 10.35FNWDD954 pKa = 3.57PPSPILSTSPEE965 pKa = 3.76QTLPLDD971 pKa = 4.23LSTSEE976 pKa = 4.65SVSTPDD982 pKa = 3.19TTSTPDD988 pKa = 2.96TVMYY992 pKa = 9.92RR993 pKa = 11.84SPPIEE998 pKa = 4.25GSQCYY1003 pKa = 9.95EE1004 pKa = 3.96EE1005 pKa = 4.8DD1006 pKa = 3.38QSDD1009 pKa = 3.84GSEE1012 pKa = 4.29SSWTSYY1018 pKa = 10.88ASSVSGCQRR1027 pKa = 11.84FDD1029 pKa = 3.37YY1030 pKa = 10.32PNRR1033 pKa = 11.84RR1034 pKa = 11.84SHH1036 pKa = 7.51PDD1038 pKa = 2.9AFPNQEE1044 pKa = 3.94AQSFRR1049 pKa = 11.84AAKK1052 pKa = 8.96EE1053 pKa = 3.46QYY1055 pKa = 9.72EE1056 pKa = 4.56RR1057 pKa = 11.84YY1058 pKa = 7.56TEE1060 pKa = 4.01SRR1062 pKa = 11.84RR1063 pKa = 11.84TQSPPQSPPPRR1074 pKa = 11.84WPTPPPPIPITEE1086 pKa = 4.3GSLTYY1091 pKa = 10.23TSVSTTTSNSKK1102 pKa = 9.97QVASGDD1108 pKa = 3.47RR1109 pKa = 11.84SRR1111 pKa = 11.84GSGAKK1116 pKa = 9.59PKK1118 pKa = 10.68VSTHH1122 pKa = 5.33SKK1124 pKa = 10.43KK1125 pKa = 9.9STDD1128 pKa = 3.36VPSQVPVKK1136 pKa = 10.2SVEE1139 pKa = 4.1VKK1141 pKa = 10.59GRR1143 pKa = 11.84TFTNSTRR1150 pKa = 11.84FSGVEE1155 pKa = 3.79HH1156 pKa = 6.8KK1157 pKa = 10.07PVKK1160 pKa = 7.75TTNGNQRR1167 pKa = 11.84VFYY1170 pKa = 10.0KK1171 pKa = 10.82VKK1173 pKa = 10.69SKK1175 pKa = 10.94DD1176 pKa = 3.37FRR1178 pKa = 11.84HH1179 pKa = 6.24PPVPPTIVGFYY1190 pKa = 10.21DD1191 pKa = 3.98GKK1193 pKa = 10.22HH1194 pKa = 5.39NYY1196 pKa = 8.57YY1197 pKa = 10.44VPTYY1201 pKa = 9.69KK1202 pKa = 10.61CQVDD1206 pKa = 3.95SKK1208 pKa = 9.22VKK1210 pKa = 10.5CVNTPPNPTKK1220 pKa = 10.62LGNFAWLGSGVSSLCAWAVTGKK1242 pKa = 10.64FEE1244 pKa = 5.13LVFDD1248 pKa = 4.95FTHH1251 pKa = 6.51QPDD1254 pKa = 3.66KK1255 pKa = 11.05MLFQCSIYY1263 pKa = 10.06IDD1265 pKa = 3.87GKK1267 pKa = 11.32LMISASRR1274 pKa = 11.84CKK1276 pKa = 10.2SKK1278 pKa = 11.16KK1279 pKa = 7.04EE1280 pKa = 3.95AKK1282 pKa = 9.91EE1283 pKa = 3.3IAAFQFLHH1291 pKa = 6.15SCFLHH1296 pKa = 5.22RR1297 pKa = 11.84TPRR1300 pKa = 11.84DD1301 pKa = 3.88LIHH1304 pKa = 6.85HH1305 pKa = 6.67ALKK1308 pKa = 9.84TPHH1311 pKa = 6.49

Molecular weight:
146.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1978

667

1311

989.0

109.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.269 ± 0.212

1.163 ± 0.207

5.561 ± 0.219

4.601 ± 0.202

4.348 ± 0.301

5.966 ± 0.242

1.517 ± 0.385

4.752 ± 0.55

4.954 ± 0.38

7.179 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.517 ± 0.235

3.488 ± 0.246

7.836 ± 0.547

4.398 ± 0.176

5.308 ± 0.407

10.111 ± 0.719

9.808 ± 1.7

6.32 ± 0.214

1.314 ± 0.208

3.589 ± 0.23

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski