Beihai sphaeromadae virus 4
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KHV5|A0A1L3KHV5_9VIRU Putative capsid protein OS=Beihai sphaeromadae virus 4 OX=1922710 PE=4 SV=1
MM1 pKa = 7.68 PSRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 IRR8 pKa = 11.84 QPIRR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 MPKK17 pKa = 10.11 RR18 pKa = 11.84 IRR20 pKa = 11.84 QPKK23 pKa = 9.08 PVTSSQTLTSTYY35 pKa = 9.88 DD36 pKa = 3.18 IPIGLPSMTAFSTKK50 pKa = 10.14 AVSLDD55 pKa = 3.47 PSAHH59 pKa = 5.58 EE60 pKa = 4.15 NSRR63 pKa = 11.84 ASAQMSIYY71 pKa = 10.86 NKK73 pKa = 10.25 YY74 pKa = 10.37 KK75 pKa = 10.83 LLACSLIYY83 pKa = 10.51 SPSVGYY89 pKa = 10.49 DD90 pKa = 3.04 SSSGLLGVFHH100 pKa = 6.55 STAEE104 pKa = 4.07 EE105 pKa = 4.16 EE106 pKa = 4.32 IAPQTRR112 pKa = 11.84 EE113 pKa = 4.0 DD114 pKa = 3.64 FQGRR118 pKa = 11.84 IQNAGPSGCLIPVRR132 pKa = 11.84 KK133 pKa = 9.84 NKK135 pKa = 9.64 VFTVPKK141 pKa = 10.21 SVIRR145 pKa = 11.84 NIAEE149 pKa = 4.67 GYY151 pKa = 8.26 TPGATQTPGRR161 pKa = 11.84 IVIGSATSCDD171 pKa = 3.19 IDD173 pKa = 4.45 SPGLLTLRR181 pKa = 11.84 STYY184 pKa = 10.15 QFIGPTVPATPASPTKK200 pKa = 9.94 IIKK203 pKa = 9.45 RR204 pKa = 11.84 APYY207 pKa = 8.69 TRR209 pKa = 11.84 RR210 pKa = 11.84 LASFVWKK217 pKa = 7.95 PTNPEE222 pKa = 3.36 WFLISGLVDD231 pKa = 3.62 SYY233 pKa = 11.89 AATSDD238 pKa = 4.02 AITDD242 pKa = 3.35 VSIEE246 pKa = 3.82 FSVYY250 pKa = 9.45 NASGSSFGKK259 pKa = 10.59 SIGQSYY265 pKa = 11.16 NDD267 pKa = 3.37 WQSFIEE273 pKa = 4.4 SAFADD278 pKa = 3.92 SQADD282 pKa = 3.45 WEE284 pKa = 4.81 TFTSSTLAAITFDD297 pKa = 3.98 PPGSPFKK304 pKa = 10.37 TAGILDD310 pKa = 3.73 SQRR313 pKa = 11.84 FVTTMPDD320 pKa = 2.99 STTTTLVDD328 pKa = 3.65 SVTTTLEE335 pKa = 3.92 EE336 pKa = 4.58 SVTTTLPLSQTTTLQDD352 pKa = 3.32 SVTTTLQEE360 pKa = 4.44 SVTTTLPEE368 pKa = 4.27 SQTTTLEE375 pKa = 4.1 DD376 pKa = 3.69 SKK378 pKa = 9.02 TTTLDD383 pKa = 3.33 SSVTTTLPQSQTTTLQGSIVKK404 pKa = 8.58 TEE406 pKa = 4.33 PGSVTTTDD414 pKa = 3.16 SDD416 pKa = 4.08 STTKK420 pKa = 9.99 TLSGSVTTTDD430 pKa = 3.71 EE431 pKa = 3.99 NSKK434 pKa = 7.89 TTTQIEE440 pKa = 4.4 SRR442 pKa = 11.84 VYY444 pKa = 10.02 IALPNQGVVSSNNPLPVDD462 pKa = 4.04 DD463 pKa = 4.91 GGGGFFMNLATGVITAFVLEE483 pKa = 4.56 KK484 pKa = 10.28 GRR486 pKa = 11.84 KK487 pKa = 8.12 YY488 pKa = 11.03 GPDD491 pKa = 3.13 SKK493 pKa = 9.86 EE494 pKa = 4.01 TPEE497 pKa = 4.53 GYY499 pKa = 10.3 VRR501 pKa = 11.84 LKK503 pKa = 9.53 GTYY506 pKa = 8.91 PAGGDD511 pKa = 3.44 VDD513 pKa = 4.19 GVSYY517 pKa = 10.92 PDD519 pKa = 3.4 VNAPSSTTSVDD530 pKa = 3.38 YY531 pKa = 11.09 KK532 pKa = 10.82 QFTSFDD538 pKa = 3.81 VLIKK542 pKa = 9.79 PQNFEE547 pKa = 3.79 GTGWHH552 pKa = 5.85 QKK554 pKa = 10.1 LDD556 pKa = 3.91 SVICRR561 pKa = 11.84 HH562 pKa = 5.5 YY563 pKa = 10.7 LGPAYY568 pKa = 10.29 YY569 pKa = 10.91 GNLQSRR575 pKa = 11.84 VAIDD579 pKa = 3.4 VTVAPILFEE588 pKa = 4.54 EE589 pKa = 5.17 ADD591 pKa = 3.72 TGNKK595 pKa = 9.38 ILGDD599 pKa = 3.62 PCRR602 pKa = 11.84 YY603 pKa = 9.13 RR604 pKa = 11.84 ALYY607 pKa = 10.31 YY608 pKa = 10.59 KK609 pKa = 9.45 DD610 pKa = 3.69 TNDD613 pKa = 2.94 FRR615 pKa = 11.84 VIRR618 pKa = 11.84 SSTEE622 pKa = 3.79 VEE624 pKa = 3.69 AKK626 pKa = 10.66 LYY628 pKa = 7.68 DD629 pKa = 3.66 TQSAIGFRR637 pKa = 11.84 VIPVMSDD644 pKa = 3.07 YY645 pKa = 10.86 PIYY648 pKa = 10.51 EE649 pKa = 4.79 DD650 pKa = 5.12 EE651 pKa = 4.45 SKK653 pKa = 8.84 TWFAIYY659 pKa = 9.87 GHH661 pKa = 6.24 NVDD664 pKa = 4.14 TNLL667 pKa = 4.15
Molecular weight: 72.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.1
IPC2_protein 5.207
IPC_protein 5.156
Toseland 5.067
ProMoST 5.296
Dawson 5.143
Bjellqvist 5.283
Wikipedia 5.041
Rodwell 5.041
Grimsley 4.991
Solomon 5.143
Lehninger 5.105
Nozaki 5.27
DTASelect 5.461
Thurlkill 5.08
EMBOSS 5.08
Sillero 5.334
Patrickios 4.012
IPC_peptide 5.156
IPC2_peptide 5.321
IPC2.peptide.svr19 5.359
Protein with the highest isoelectric point:
>tr|A0A1L3KHV5|A0A1L3KHV5_9VIRU Putative capsid protein OS=Beihai sphaeromadae virus 4 OX=1922710 PE=4 SV=1
MM1 pKa = 7.76 ASFSNPVNASEE12 pKa = 4.43 KK13 pKa = 10.62 LSGASFLAVATRR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 QDD30 pKa = 3.05 LSFLEE35 pKa = 4.52 GQLDD39 pKa = 3.38 KK40 pKa = 10.59 CTRR43 pKa = 11.84 LSPQFHH49 pKa = 6.55 LPIRR53 pKa = 11.84 DD54 pKa = 3.24 IPEE57 pKa = 3.87 IKK59 pKa = 9.44 STGKK63 pKa = 11.03 GDD65 pKa = 3.41 MQRR68 pKa = 11.84 AKK70 pKa = 9.41 TQTFDD75 pKa = 3.82 VPDD78 pKa = 4.05 NFEE81 pKa = 4.59 KK82 pKa = 10.88 LPPIPNSKK90 pKa = 8.61 LTRR93 pKa = 11.84 NGEE96 pKa = 4.3 LIQKK100 pKa = 9.97 GNLGKK105 pKa = 10.37 RR106 pKa = 11.84 FYY108 pKa = 11.09 NSQKK112 pKa = 9.23 TFGGKK117 pKa = 8.8 NVPDD121 pKa = 4.13 NLRR124 pKa = 11.84 QTIRR128 pKa = 11.84 HH129 pKa = 5.59 ALATRR134 pKa = 11.84 IEE136 pKa = 4.38 INGPAVEE143 pKa = 4.39 SCVIADD149 pKa = 3.71 EE150 pKa = 4.78 LMATGLEE157 pKa = 4.2 WEE159 pKa = 4.59 YY160 pKa = 11.59 SAGTTQGLVEE170 pKa = 4.02 RR171 pKa = 11.84 LSRR174 pKa = 11.84 FGKK177 pKa = 10.05 DD178 pKa = 2.92 DD179 pKa = 3.29 KK180 pKa = 10.83 TVSFFRR186 pKa = 11.84 QTDD189 pKa = 3.91 EE190 pKa = 3.57 IRR192 pKa = 11.84 NFQSFLFQALDD203 pKa = 3.28 TLLPLKK209 pKa = 9.03 STPFEE214 pKa = 3.91 TSFPSTSTEE223 pKa = 3.9 VIEE226 pKa = 4.97 GIRR229 pKa = 11.84 VNPQSSAGPMYY240 pKa = 9.96 GTTKK244 pKa = 10.39 VQALDD249 pKa = 5.17 DD250 pKa = 3.73 IMLTMDD256 pKa = 5.59 AIRR259 pKa = 11.84 TQFSLGTLDD268 pKa = 3.53 TFFAAHH274 pKa = 7.51 PEE276 pKa = 4.04 TLIADD281 pKa = 4.28 CRR283 pKa = 11.84 NKK285 pKa = 9.38 TDD287 pKa = 3.18 RR288 pKa = 11.84 YY289 pKa = 9.91 EE290 pKa = 3.98 IAKK293 pKa = 10.14 LPEE296 pKa = 3.66 KK297 pKa = 9.66 TRR299 pKa = 11.84 PYY301 pKa = 10.33 WSFNAHH307 pKa = 3.89 WQFYY311 pKa = 8.49 YY312 pKa = 10.41 SWLMQRR318 pKa = 11.84 FCDD321 pKa = 3.84 NLYY324 pKa = 9.98 VASDD328 pKa = 4.16 PEE330 pKa = 4.95 CDD332 pKa = 3.31 VPTYY336 pKa = 10.94 NYY338 pKa = 9.99 YY339 pKa = 10.07 GHH341 pKa = 6.53 VWAKK345 pKa = 10.64 GGAQRR350 pKa = 11.84 IHH352 pKa = 6.17 EE353 pKa = 4.83 SIMNVAKK360 pKa = 10.01 TGRR363 pKa = 11.84 PFVGIYY369 pKa = 10.82 GDD371 pKa = 4.08 DD372 pKa = 3.63 FLFAFPIKK380 pKa = 9.4 TDD382 pKa = 3.25 EE383 pKa = 4.27 GTTIQVMAPDD393 pKa = 4.43 ISQMDD398 pKa = 3.94 STVSHH403 pKa = 5.93 AVIRR407 pKa = 11.84 GTCDD411 pKa = 3.23 YY412 pKa = 10.54 IYY414 pKa = 10.71 KK415 pKa = 10.12 QYY417 pKa = 11.08 QEE419 pKa = 4.29 AHH421 pKa = 6.44 GDD423 pKa = 3.65 DD424 pKa = 3.76 PFVRR428 pKa = 11.84 FLLTRR433 pKa = 11.84 LAEE436 pKa = 4.57 DD437 pKa = 3.34 ASSPRR442 pKa = 11.84 FLASAIATYY451 pKa = 10.49 QNQRR455 pKa = 11.84 PTGLATGVVGTTLFDD470 pKa = 3.64 TVQAALLAFSVIEE483 pKa = 3.68 EE484 pKa = 4.11 WNRR487 pKa = 11.84 TKK489 pKa = 10.84 RR490 pKa = 11.84 SPDD493 pKa = 3.23 EE494 pKa = 3.99 IFNKK498 pKa = 9.52 VSKK501 pKa = 10.43 KK502 pKa = 10.02 FGMRR506 pKa = 11.84 AKK508 pKa = 10.42 SLEE511 pKa = 3.94 LYY513 pKa = 9.45 EE514 pKa = 4.55 YY515 pKa = 10.94 NGLPPAEE522 pKa = 4.08 GTEE525 pKa = 4.08 WTFLGMQLATYY536 pKa = 10.49 SNSKK540 pKa = 7.91 HH541 pKa = 5.35 TNGVIPRR548 pKa = 11.84 LPRR551 pKa = 11.84 DD552 pKa = 3.56 SLLKK556 pKa = 10.72 SILNPRR562 pKa = 11.84 INSYY566 pKa = 10.77 LFSSKK571 pKa = 10.64 LGTQRR576 pKa = 11.84 YY577 pKa = 8.91 LFDD580 pKa = 5.0 ASRR583 pKa = 11.84 GWYY586 pKa = 9.86 LCALGDD592 pKa = 3.78 RR593 pKa = 11.84 LIWDD597 pKa = 3.73 VLSSVIEE604 pKa = 4.27 TVPTMAVGMVVQAGGGLGAPPEE626 pKa = 4.46 CNGLFDD632 pKa = 4.79 VNRR635 pKa = 11.84 PFNWLSSFGVPTMDD649 pKa = 2.77 WVADD653 pKa = 4.05 LFLSPMDD660 pKa = 4.97 PDD662 pKa = 3.78 PAGWLSLLDD671 pKa = 3.86 PPKK674 pKa = 10.78 GRR676 pKa = 11.84 LIVEE680 pKa = 4.29 MQDD683 pKa = 3.26 QVDD686 pKa = 4.11 LSRR689 pKa = 11.84 YY690 pKa = 6.89 TIRR693 pKa = 11.84 PTPNTAPHH701 pKa = 5.44 SWEE704 pKa = 3.81 TRR706 pKa = 11.84 EE707 pKa = 4.99 KK708 pKa = 10.13 VTDD711 pKa = 3.62 TNLEE715 pKa = 4.19 SLVSDD720 pKa = 3.88 NPSGLGPQVQRR731 pKa = 11.84 PKK733 pKa = 10.51 AQHH736 pKa = 6.23 LPPVKK741 pKa = 10.41 APPFKK746 pKa = 10.41 IRR748 pKa = 11.84 LSEE751 pKa = 4.1 HH752 pKa = 6.27 LSKK755 pKa = 11.18 HH756 pKa = 5.96 NITAFVPSEE765 pKa = 3.76 LAAEE769 pKa = 4.53 LGVSVTTILTEE780 pKa = 4.41 LYY782 pKa = 10.45 SKK784 pKa = 10.52 EE785 pKa = 4.33 RR786 pKa = 11.84 EE787 pKa = 4.06 TKK789 pKa = 9.72 APEE792 pKa = 3.78 FRR794 pKa = 11.84 WYY796 pKa = 10.51 SLGRR800 pKa = 11.84 ILTKK804 pKa = 10.9 DD805 pKa = 3.29 PVDD808 pKa = 3.55 TVQPSRR814 pKa = 11.84 ILWNQYY820 pKa = 9.05 SRR822 pKa = 11.84 ILSEE826 pKa = 4.63 LGYY829 pKa = 10.34 SNPAIQPARR838 pKa = 11.84 PKK840 pKa = 10.27 AGPVPAPRR848 pKa = 11.84 YY849 pKa = 10.25 SKK851 pKa = 10.26 MKK853 pKa = 10.78 ANVPQDD859 pKa = 3.47 FATSNSTTISDD870 pKa = 4.63 CDD872 pKa = 3.4 TSPAIRR878 pKa = 11.84 VSGPDD883 pKa = 3.23 QTPTSQTVPQYY894 pKa = 9.79 TPKK897 pKa = 9.26 PAQMMYY903 pKa = 10.64 QRR905 pKa = 11.84 IPAKK909 pKa = 9.83 TGAVVCALEE918 pKa = 4.36 EE919 pKa = 4.08 NLVAPRR925 pKa = 11.84 AEE927 pKa = 4.3 GLSSGARR934 pKa = 11.84 VPLADD939 pKa = 3.46 QVNSGRR945 pKa = 11.84 IVHH948 pKa = 6.36 QYY950 pKa = 10.35 FNWDD954 pKa = 3.57 PPSPILSTSPEE965 pKa = 3.76 QTLPLDD971 pKa = 4.23 LSTSEE976 pKa = 4.65 SVSTPDD982 pKa = 3.19 TTSTPDD988 pKa = 2.96 TVMYY992 pKa = 9.92 RR993 pKa = 11.84 SPPIEE998 pKa = 4.25 GSQCYY1003 pKa = 9.95 EE1004 pKa = 3.96 EE1005 pKa = 4.8 DD1006 pKa = 3.38 QSDD1009 pKa = 3.84 GSEE1012 pKa = 4.29 SSWTSYY1018 pKa = 10.88 ASSVSGCQRR1027 pKa = 11.84 FDD1029 pKa = 3.37 YY1030 pKa = 10.32 PNRR1033 pKa = 11.84 RR1034 pKa = 11.84 SHH1036 pKa = 7.51 PDD1038 pKa = 2.9 AFPNQEE1044 pKa = 3.94 AQSFRR1049 pKa = 11.84 AAKK1052 pKa = 8.96 EE1053 pKa = 3.46 QYY1055 pKa = 9.72 EE1056 pKa = 4.56 RR1057 pKa = 11.84 YY1058 pKa = 7.56 TEE1060 pKa = 4.01 SRR1062 pKa = 11.84 RR1063 pKa = 11.84 TQSPPQSPPPRR1074 pKa = 11.84 WPTPPPPIPITEE1086 pKa = 4.3 GSLTYY1091 pKa = 10.23 TSVSTTTSNSKK1102 pKa = 9.97 QVASGDD1108 pKa = 3.47 RR1109 pKa = 11.84 SRR1111 pKa = 11.84 GSGAKK1116 pKa = 9.59 PKK1118 pKa = 10.68 VSTHH1122 pKa = 5.33 SKK1124 pKa = 10.43 KK1125 pKa = 9.9 STDD1128 pKa = 3.36 VPSQVPVKK1136 pKa = 10.2 SVEE1139 pKa = 4.1 VKK1141 pKa = 10.59 GRR1143 pKa = 11.84 TFTNSTRR1150 pKa = 11.84 FSGVEE1155 pKa = 3.79 HH1156 pKa = 6.8 KK1157 pKa = 10.07 PVKK1160 pKa = 7.75 TTNGNQRR1167 pKa = 11.84 VFYY1170 pKa = 10.0 KK1171 pKa = 10.82 VKK1173 pKa = 10.69 SKK1175 pKa = 10.94 DD1176 pKa = 3.37 FRR1178 pKa = 11.84 HH1179 pKa = 6.24 PPVPPTIVGFYY1190 pKa = 10.21 DD1191 pKa = 3.98 GKK1193 pKa = 10.22 HH1194 pKa = 5.39 NYY1196 pKa = 8.57 YY1197 pKa = 10.44 VPTYY1201 pKa = 9.69 KK1202 pKa = 10.61 CQVDD1206 pKa = 3.95 SKK1208 pKa = 9.22 VKK1210 pKa = 10.5 CVNTPPNPTKK1220 pKa = 10.62 LGNFAWLGSGVSSLCAWAVTGKK1242 pKa = 10.64 FEE1244 pKa = 5.13 LVFDD1248 pKa = 4.95 FTHH1251 pKa = 6.51 QPDD1254 pKa = 3.66 KK1255 pKa = 11.05 MLFQCSIYY1263 pKa = 10.06 IDD1265 pKa = 3.87 GKK1267 pKa = 11.32 LMISASRR1274 pKa = 11.84 CKK1276 pKa = 10.2 SKK1278 pKa = 11.16 KK1279 pKa = 7.04 EE1280 pKa = 3.95 AKK1282 pKa = 9.91 EE1283 pKa = 3.3 IAAFQFLHH1291 pKa = 6.15 SCFLHH1296 pKa = 5.22 RR1297 pKa = 11.84 TPRR1300 pKa = 11.84 DD1301 pKa = 3.88 LIHH1304 pKa = 6.85 HH1305 pKa = 6.67 ALKK1308 pKa = 9.84 TPHH1311 pKa = 6.49
Molecular weight: 146.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.417
IPC2_protein 7.819
IPC_protein 7.629
Toseland 7.38
ProMoST 8.214
Dawson 8.404
Bjellqvist 8.682
Wikipedia 8.346
Rodwell 8.448
Grimsley 7.439
Solomon 8.448
Lehninger 8.448
Nozaki 8.814
DTASelect 8.448
Thurlkill 8.507
EMBOSS 8.595
Sillero 8.799
Patrickios 4.431
IPC_peptide 8.434
IPC2_peptide 7.512
IPC2.peptide.svr19 7.793
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1978
667
1311
989.0
109.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.269 ± 0.212
1.163 ± 0.207
5.561 ± 0.219
4.601 ± 0.202
4.348 ± 0.301
5.966 ± 0.242
1.517 ± 0.385
4.752 ± 0.55
4.954 ± 0.38
7.179 ± 0.368
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.517 ± 0.235
3.488 ± 0.246
7.836 ± 0.547
4.398 ± 0.176
5.308 ± 0.407
10.111 ± 0.719
9.808 ± 1.7
6.32 ± 0.214
1.314 ± 0.208
3.589 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here