Maribacter polysiphoniae
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4431 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A316E4N3|A0A316E4N3_9FLAO 6-phosphogluconolactonase OS=Maribacter polysiphoniae OX=429344 GN=LX92_00103 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 10.04 PILRR6 pKa = 11.84 TVMFGNSKK14 pKa = 9.81 MISHH18 pKa = 7.24 KK19 pKa = 10.99 SFVCIIGVFIFFGFQNIFSQTTFTEE44 pKa = 4.47 TAASFNLDD52 pKa = 2.6 IGGNKK57 pKa = 10.14 DD58 pKa = 4.18 GGHH61 pKa = 6.55 AWADD65 pKa = 3.46 YY66 pKa = 11.53 DD67 pKa = 4.47 NDD69 pKa = 3.63 GDD71 pKa = 4.78 LDD73 pKa = 4.01 VLVLEE78 pKa = 4.73 NVNGSTRR85 pKa = 11.84 SYY87 pKa = 11.5 LMRR90 pKa = 11.84 NNGNNTFTDD99 pKa = 3.26 VRR101 pKa = 11.84 AALITGMGNDD111 pKa = 3.23 VAEE114 pKa = 4.62 RR115 pKa = 11.84 QAAWGDD121 pKa = 3.68 LNNDD125 pKa = 3.09 GRR127 pKa = 11.84 PDD129 pKa = 3.69 FMITSSGTSGGGGKK143 pKa = 9.77 AALQIYY149 pKa = 7.94 LQNTSGTFGDD159 pKa = 4.27 GAGGNTPITVGRR171 pKa = 11.84 GSFTININPINAEE184 pKa = 3.71 GAGFFDD190 pKa = 5.29 FEE192 pKa = 5.45 GDD194 pKa = 3.18 GDD196 pKa = 4.44 LDD198 pKa = 4.12 IFFDD202 pKa = 3.52 SHH204 pKa = 7.59 NYY206 pKa = 9.96 GIEE209 pKa = 4.0 LLRR212 pKa = 11.84 NNYY215 pKa = 8.89 IDD217 pKa = 3.91 HH218 pKa = 5.76 TTSTIVNPPAASLFTHH234 pKa = 6.22 ITTGNGTGVVNFGLNQFATDD254 pKa = 3.52 GDD256 pKa = 4.24 YY257 pKa = 11.67 GSAADD262 pKa = 4.13 VNDD265 pKa = 5.14 DD266 pKa = 3.27 GWVDD270 pKa = 2.55 IFMRR274 pKa = 11.84 KK275 pKa = 8.61 RR276 pKa = 11.84 DD277 pKa = 3.74 EE278 pKa = 4.48 NDD280 pKa = 2.67 FFLNQGGTFTNGADD294 pKa = 3.59 LAQAEE299 pKa = 4.4 NGNKK303 pKa = 9.74 GGNGLWDD310 pKa = 4.35 LDD312 pKa = 3.81 NDD314 pKa = 4.14 GDD316 pKa = 5.06 LDD318 pKa = 4.86 AVWTEE323 pKa = 3.75 NGFTQIYY330 pKa = 10.08 RR331 pKa = 11.84 NDD333 pKa = 3.65 AGTWTPLGAAVFPGLPQPSSSNSGASSARR362 pKa = 11.84 IDD364 pKa = 3.59 ALAGGDD370 pKa = 3.72 IDD372 pKa = 4.66 NDD374 pKa = 3.56 GDD376 pKa = 3.96 IDD378 pKa = 3.7 ILLVGRR384 pKa = 11.84 NRR386 pKa = 11.84 SYY388 pKa = 11.62 LYY390 pKa = 10.21 INQLNSPTPAPGVVGSGTAMSFSLDD415 pKa = 3.37 SQTFNSGRR423 pKa = 11.84 DD424 pKa = 3.82 GEE426 pKa = 4.7 GTTMVDD432 pKa = 3.05 VDD434 pKa = 5.18 DD435 pKa = 6.66 DD436 pKa = 4.8 GDD438 pKa = 4.09 LDD440 pKa = 3.71 IYY442 pKa = 11.07 MNINGNKK449 pKa = 9.46 NKK451 pKa = 10.45 LYY453 pKa = 10.8 LNNLPAANRR462 pKa = 11.84 KK463 pKa = 8.21 NHH465 pKa = 6.3 LLVDD469 pKa = 3.67 VTEE472 pKa = 5.02 DD473 pKa = 3.41 RR474 pKa = 11.84 DD475 pKa = 4.04 ANGNTGGFPGRR486 pKa = 11.84 VAIGTNVLIRR496 pKa = 11.84 DD497 pKa = 3.78 CAGNIVSGLRR507 pKa = 11.84 QVNGVYY513 pKa = 10.51 GHH515 pKa = 6.48 GTQQPEE521 pKa = 4.14 EE522 pKa = 4.14 VHH524 pKa = 6.65 FGLPLGEE531 pKa = 4.25 GEE533 pKa = 4.25 TYY535 pKa = 10.03 IIEE538 pKa = 3.91 VHH540 pKa = 5.88 YY541 pKa = 10.53 PNFNDD546 pKa = 3.58 PVNGITRR553 pKa = 11.84 LIATAIAQPSTIPGTNHH570 pKa = 6.21 YY571 pKa = 11.2 SLTTTNAEE579 pKa = 4.45 LIEE582 pKa = 4.55 NPNAPIANDD591 pKa = 3.67 DD592 pKa = 4.34 LKK594 pKa = 10.38 TVAHH598 pKa = 6.75 GNTVSVQISLFDD610 pKa = 3.98 NDD612 pKa = 4.68 SEE614 pKa = 4.52 PDD616 pKa = 3.49 GEE618 pKa = 4.4 NFFIEE623 pKa = 5.18 SVVQPAIGSVVIDD636 pKa = 4.44 DD637 pKa = 4.42 ADD639 pKa = 4.05 AGLVTYY645 pKa = 8.31 TYY647 pKa = 11.06 SAATPFSGTTFDD659 pKa = 3.79 YY660 pKa = 10.62 TITDD664 pKa = 3.65 ATSNLCPAQGKK675 pKa = 10.04 SDD677 pKa = 3.69 TATVTIFEE685 pKa = 4.94 PCTDD689 pKa = 3.42 SSGLDD694 pKa = 3.3 TDD696 pKa = 4.59 GDD698 pKa = 4.2 GFNNVCDD705 pKa = 5.39 LDD707 pKa = 4.79 DD708 pKa = 5.79 DD709 pKa = 4.42 NDD711 pKa = 4.93 GILDD715 pKa = 3.59 VVEE718 pKa = 4.37 RR719 pKa = 11.84 PKK721 pKa = 10.61 TVLWVLDD728 pKa = 3.69 GTITPDD734 pKa = 3.22 QQNVIDD740 pKa = 4.56 KK741 pKa = 8.04 LTNLGYY747 pKa = 8.91 TLTLADD753 pKa = 5.56 DD754 pKa = 4.77 NDD756 pKa = 4.12 SQDD759 pKa = 3.31 ANNYY763 pKa = 8.63 SVTYY767 pKa = 7.1 VHH769 pKa = 7.07 PSVSSGTAFANISNLATTTNGVITSEE795 pKa = 3.82 NALFDD800 pKa = 4.15 EE801 pKa = 5.2 LFGTSGAVGNPTTNLINIINNTHH824 pKa = 7.7 PITLSLPLGNLDD836 pKa = 3.3 IGSGDD841 pKa = 3.64 FYY843 pKa = 11.44 VNNVVTGTKK852 pKa = 10.25 LGQHH856 pKa = 7.14 PDD858 pKa = 2.99 GTANLIAWEE867 pKa = 4.24 VGEE870 pKa = 5.52 AMDD873 pKa = 4.45 TGIAPGRR880 pKa = 11.84 RR881 pKa = 11.84 VAAPHH886 pKa = 6.19 TSHH889 pKa = 7.94 DD890 pKa = 3.81 GGLNSAGEE898 pKa = 4.36 DD899 pKa = 3.62 LLVSAILWTWALDD912 pKa = 3.61 TDD914 pKa = 4.78 GDD916 pKa = 4.25 GLYY919 pKa = 10.75 DD920 pKa = 5.89 DD921 pKa = 6.16 LDD923 pKa = 5.1 LDD925 pKa = 4.2 SDD927 pKa = 4.4 GDD929 pKa = 4.24 GIPDD933 pKa = 3.41 NVEE936 pKa = 3.69 AQTTLTYY943 pKa = 9.85 TPPNGDD949 pKa = 3.17 SNAIYY954 pKa = 10.21 AANNGVNSSYY964 pKa = 11.07 LGGLLPVNTDD974 pKa = 2.95 GADD977 pKa = 3.32 NPDD980 pKa = 3.95 YY981 pKa = 11.48 LDD983 pKa = 3.58 VDD985 pKa = 4.3 SDD987 pKa = 4.37 NEE989 pKa = 4.26 GHH991 pKa = 7.35 GDD993 pKa = 3.38 TTEE996 pKa = 4.14 AGIALAGVDD1005 pKa = 4.16 TDD1007 pKa = 5.51 NDD1009 pKa = 4.14 GLDD1012 pKa = 4.2 DD1013 pKa = 6.76 GIDD1016 pKa = 3.72 TDD1018 pKa = 3.97 LTGYY1022 pKa = 10.42 DD1023 pKa = 4.37 DD1024 pKa = 4.75 PGGTIDD1030 pKa = 6.08 DD1031 pKa = 4.71 PLNTPLTLLDD1041 pKa = 3.88 TDD1043 pKa = 4.02 NDD1045 pKa = 3.68 ATTGGDD1051 pKa = 3.12 VDD1053 pKa = 5.74 FRR1055 pKa = 11.84 DD1056 pKa = 4.3 ALDD1059 pKa = 4.51 DD1060 pKa = 5.0 RR1061 pKa = 11.84 PDD1063 pKa = 3.44 NDD1065 pKa = 4.32 LDD1067 pKa = 4.72 GIVDD1071 pKa = 4.7 AEE1073 pKa = 5.57 DD1074 pKa = 4.48 FDD1076 pKa = 6.6 DD1077 pKa = 6.12 DD1078 pKa = 4.63 NDD1080 pKa = 4.96 GILDD1084 pKa = 3.58 IDD1086 pKa = 4.07 EE1087 pKa = 4.84 GCGNLVVNGNFEE1099 pKa = 4.22 AQDD1102 pKa = 3.45 FSDD1105 pKa = 4.58 AVEE1108 pKa = 4.45 FPNGFTEE1115 pKa = 4.91 AGGTFIGATYY1125 pKa = 8.14 NTNPLTGWSYY1135 pKa = 8.06 TQNMDD1140 pKa = 2.67 GWVGNQRR1147 pKa = 11.84 MSWSSNDD1154 pKa = 2.97 FAPAYY1159 pKa = 9.92 KK1160 pKa = 9.95 GGQYY1164 pKa = 10.08 IDD1166 pKa = 4.33 VIGNNAASGGSNNVLTQIINTEE1188 pKa = 3.89 PGEE1191 pKa = 4.32 TYY1193 pKa = 10.09 TFSFFWGEE1201 pKa = 4.16 DD1202 pKa = 3.02 VGHH1205 pKa = 6.6 RR1206 pKa = 11.84 VGDD1209 pKa = 3.73 PVILNVSVLDD1219 pKa = 3.83 SGSNSLMNQTLNTTAFGIVDD1239 pKa = 4.48 NIVGPQNWYY1248 pKa = 10.22 YY1249 pKa = 11.01 FEE1251 pKa = 4.04 QTFIATTNTTTLRR1264 pKa = 11.84 FAATPSGTANGTAIDD1279 pKa = 3.82 FVYY1282 pKa = 9.35 VTKK1285 pKa = 10.98 NGLCQDD1291 pKa = 3.43 TDD1293 pKa = 3.55 GDD1295 pKa = 4.44 GVIDD1299 pKa = 5.66 AFDD1302 pKa = 4.98 LDD1304 pKa = 3.98 SDD1306 pKa = 3.86 NDD1308 pKa = 4.58 GIYY1311 pKa = 10.79 DD1312 pKa = 3.63 AVEE1315 pKa = 4.32 AGHH1318 pKa = 6.74 GAAHH1322 pKa = 6.4 TNGVVNGAIGTDD1334 pKa = 3.88 GVPDD1338 pKa = 4.22 AVQSTPNNEE1347 pKa = 3.73 MVNYY1351 pKa = 9.61 IVEE1354 pKa = 4.87 DD1355 pKa = 3.73 SDD1357 pKa = 4.35 SDD1359 pKa = 3.97 GSIDD1363 pKa = 5.41 AIEE1366 pKa = 4.74 SDD1368 pKa = 5.19 ADD1370 pKa = 3.93 DD1371 pKa = 5.48 DD1372 pKa = 4.32 GCNDD1376 pKa = 3.23 VDD1378 pKa = 3.76 EE1379 pKa = 5.71 AGYY1382 pKa = 9.31 TDD1384 pKa = 3.94 GNNDD1388 pKa = 3.33 GLLGPNPITVDD1399 pKa = 3.3 GNGLVISGTDD1409 pKa = 3.66 GYY1411 pKa = 7.04 TTPADD1416 pKa = 3.46 ANTNSTYY1423 pKa = 11.16 DD1424 pKa = 3.52 FQEE1427 pKa = 4.4 AGSPASIVGQPSNITICPGCTGEE1450 pKa = 4.7 IKK1452 pKa = 9.5 ITGTDD1457 pKa = 2.62 IDD1459 pKa = 4.17 TYY1461 pKa = 10.68 QWQLFDD1467 pKa = 3.93 GGSWMDD1473 pKa = 3.88 LSDD1476 pKa = 3.32 GGIYY1480 pKa = 10.26 SGTATDD1486 pKa = 4.95 SLIITNPTTSDD1497 pKa = 2.45 TGNQYY1502 pKa = 10.58 RR1503 pKa = 11.84 VIVSNSAFVCSGDD1516 pKa = 3.22 ISATGVLTVAAKK1528 pKa = 9.49 TIITNRR1534 pKa = 11.84 RR1535 pKa = 11.84 ITYY1538 pKa = 9.63 RR1539 pKa = 11.84 VNKK1542 pKa = 9.67 NN1543 pKa = 2.78
Molecular weight: 162.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.554
IPC_protein 3.63
Toseland 3.376
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.605
Rodwell 3.452
Grimsley 3.287
Solomon 3.643
Lehninger 3.605
Nozaki 3.745
DTASelect 4.075
Thurlkill 3.439
EMBOSS 3.617
Sillero 3.757
Patrickios 1.481
IPC_peptide 3.63
IPC2_peptide 3.732
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A316E630|A0A316E630_9FLAO Copper chaperone CopZ OS=Maribacter polysiphoniae OX=429344 GN=LX92_01750 PE=4 SV=1
MM1 pKa = 7.21 SRR3 pKa = 11.84 EE4 pKa = 3.57 RR5 pKa = 11.84 RR6 pKa = 11.84 KK7 pKa = 10.21 RR8 pKa = 11.84 RR9 pKa = 11.84 LEE11 pKa = 3.96 EE12 pKa = 3.88 KK13 pKa = 10.17 KK14 pKa = 10.85 GNSINRR20 pKa = 11.84 KK21 pKa = 7.73 VRR23 pKa = 11.84 TRR25 pKa = 11.84 KK26 pKa = 9.57 EE27 pKa = 3.43 KK28 pKa = 10.59 GYY30 pKa = 10.43 CLLVFVAMFLAVLTIAFLDD49 pKa = 4.49 AILYY53 pKa = 9.21 GFYY56 pKa = 11.18 LLFNN60 pKa = 4.88
Molecular weight: 7.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.809
IPC_protein 10.482
Toseland 10.701
ProMoST 10.35
Dawson 10.804
Bjellqvist 10.482
Wikipedia 10.994
Rodwell 11.14
Grimsley 10.862
Solomon 10.891
Lehninger 10.862
Nozaki 10.672
DTASelect 10.482
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.73
Patrickios 10.906
IPC_peptide 10.906
IPC2_peptide 9.37
IPC2.peptide.svr19 8.524
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4431
0
4431
1523809
39
5722
343.9
38.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.416 ± 0.035
0.739 ± 0.011
5.891 ± 0.029
6.51 ± 0.036
5.039 ± 0.031
6.925 ± 0.035
1.889 ± 0.019
7.575 ± 0.038
7.439 ± 0.055
9.348 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.416 ± 0.02
5.748 ± 0.035
3.615 ± 0.022
3.271 ± 0.017
3.593 ± 0.025
6.284 ± 0.03
5.755 ± 0.045
6.272 ± 0.027
1.172 ± 0.015
4.104 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here