Streptococcus phage SFi18

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9MCH6|Q9MCH6_9CAUD Lysin OS=Streptococcus phage SFi18 OX=74382 PE=4 SV=1
MM1 pKa = 6.73TRR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 8.53TKK7 pKa = 10.27MNVPQSLVNWFVNHH21 pKa = 6.78RR22 pKa = 11.84NLLTYY27 pKa = 11.07SMYY30 pKa = 10.88GSRR33 pKa = 11.84NGSDD37 pKa = 2.98GTADD41 pKa = 3.56CSGSMSQALKK51 pKa = 10.31EE52 pKa = 3.98AGIPIQGLPSTVTLGQQLAKK72 pKa = 10.58NGFYY76 pKa = 10.45RR77 pKa = 11.84ISRR80 pKa = 11.84NEE82 pKa = 3.68DD83 pKa = 2.64WNAEE87 pKa = 4.08TGDD90 pKa = 3.78IVLMSWGADD99 pKa = 3.28MASSGGAGGHH109 pKa = 5.79VGVMMDD115 pKa = 3.26SVNFISCDD123 pKa = 3.35YY124 pKa = 8.86STQGAAGQAINTYY137 pKa = 8.75PWNDD141 pKa = 3.22YY142 pKa = 11.23YY143 pKa = 11.03EE144 pKa = 4.53ANKK147 pKa = 9.11PAYY150 pKa = 9.19IEE152 pKa = 3.71VWRR155 pKa = 11.84YY156 pKa = 10.23SEE158 pKa = 5.29SAPQTKK164 pKa = 9.54NQANTAVTPQQKK176 pKa = 10.45AYY178 pKa = 11.02YY179 pKa = 8.08EE180 pKa = 4.27ANEE183 pKa = 3.96VKK185 pKa = 10.25YY186 pKa = 11.21VNGIWQIKK194 pKa = 9.37CDD196 pKa = 4.03YY197 pKa = 9.83LSPIGFDD204 pKa = 3.37YY205 pKa = 10.85LGNGIPVTMVNWVDD219 pKa = 3.69KK220 pKa = 11.03DD221 pKa = 4.24GNDD224 pKa = 3.97LPDD227 pKa = 4.77GADD230 pKa = 3.18QDD232 pKa = 4.92FKK234 pKa = 11.67AGMYY238 pKa = 10.22FSFSSDD244 pKa = 2.71EE245 pKa = 4.23TNIVDD250 pKa = 3.86TGNGGYY256 pKa = 10.39YY257 pKa = 10.01GGYY260 pKa = 7.73YY261 pKa = 8.88WRR263 pKa = 11.84LFEE266 pKa = 5.07FGQFGPVWLSCWNKK280 pKa = 10.66DD281 pKa = 3.89DD282 pKa = 5.14LVNYY286 pKa = 7.38YY287 pKa = 8.26QQ288 pKa = 4.22

Molecular weight: 32.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q77PS4|Q77PS4_9CAUD Gp143 OS=Streptococcus phage SFi18 OX=74382 PE=4 SV=1
MM1 pKa = 7.17ATEE4 pKa = 5.11SLIQNQIRR12 pKa = 11.84VGLSKK17 pKa = 10.65AGYY20 pKa = 7.78MVFRR24 pKa = 11.84INVGKK29 pKa = 10.59VRR31 pKa = 11.84MADD34 pKa = 3.45GRR36 pKa = 11.84WFDD39 pKa = 3.37TGAPKK44 pKa = 10.71GFCDD48 pKa = 4.64LFGFRR53 pKa = 11.84PDD55 pKa = 3.23GQIFFIEE62 pKa = 4.32VKK64 pKa = 10.39NEE66 pKa = 3.5KK67 pKa = 10.23GRR69 pKa = 11.84LRR71 pKa = 11.84EE72 pKa = 4.04DD73 pKa = 2.94QKK75 pKa = 11.97KK76 pKa = 10.1FMNAMKK82 pKa = 10.49KK83 pKa = 9.89RR84 pKa = 11.84GALVGVARR92 pKa = 11.84SVKK95 pKa = 9.72EE96 pKa = 3.58AMDD99 pKa = 3.38IVNEE103 pKa = 4.15KK104 pKa = 10.5QSS106 pKa = 3.17

Molecular weight: 12.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

4200

40

504

161.5

18.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.429 ± 0.384

1.024 ± 0.221

6.524 ± 0.339

7.952 ± 0.548

4.095 ± 0.328

6.0 ± 0.568

1.571 ± 0.206

6.857 ± 0.421

9.333 ± 0.652

7.643 ± 0.518

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.714 ± 0.309

6.333 ± 0.372

2.833 ± 0.401

3.238 ± 0.278

4.5 ± 0.363

5.048 ± 0.306

5.143 ± 0.45

6.548 ± 0.496

1.881 ± 0.272

5.333 ± 0.4
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski