Peanut clump virus (isolate 87/TGTA2) (PCV)
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q08552|TGB3_PCV87 Movement protein TGB3 OS=Peanut clump virus (isolate 87/TGTA2) OX=652837 PE=3 SV=1
MM1 pKa = 6.91 EE2 pKa = 4.07 WRR4 pKa = 11.84 RR5 pKa = 11.84 AFSKK9 pKa = 10.69 KK10 pKa = 10.1 KK11 pKa = 10.65 LEE13 pKa = 4.16 EE14 pKa = 3.89 KK15 pKa = 9.96 VKK17 pKa = 11.15 NMDD20 pKa = 3.12 AKK22 pKa = 10.77 QATDD26 pKa = 3.53 YY27 pKa = 11.43 LLEE30 pKa = 4.52 KK31 pKa = 10.52 VDD33 pKa = 3.66 EE34 pKa = 4.15 QRR36 pKa = 11.84 NLEE39 pKa = 4.11 NKK41 pKa = 9.77 LDD43 pKa = 3.71 KK44 pKa = 10.74 RR45 pKa = 11.84 LQNTRR50 pKa = 11.84 KK51 pKa = 9.85 KK52 pKa = 10.61 NKK54 pKa = 9.47 NKK56 pKa = 10.31 EE57 pKa = 4.05 KK58 pKa = 9.65 TRR60 pKa = 11.84 TWAEE64 pKa = 3.74 KK65 pKa = 10.23 YY66 pKa = 10.37 PPVDD70 pKa = 3.33 YY71 pKa = 10.94 YY72 pKa = 11.63 SPEE75 pKa = 3.77 FVEE78 pKa = 5.85 NFMKK82 pKa = 10.7 DD83 pKa = 3.03 MRR85 pKa = 11.84 RR86 pKa = 11.84 EE87 pKa = 3.8 EE88 pKa = 4.2 FEE90 pKa = 3.83 KK91 pKa = 10.48 SEE93 pKa = 3.93 EE94 pKa = 4.15 RR95 pKa = 11.84 RR96 pKa = 11.84 GHH98 pKa = 4.84 KK99 pKa = 8.02 QVRR102 pKa = 11.84 LGSDD106 pKa = 3.15 NFVGDD111 pKa = 4.55 DD112 pKa = 3.66 PLKK115 pKa = 10.68 VLSEE119 pKa = 4.23 EE120 pKa = 3.92 ALKK123 pKa = 11.01 AGFQHH128 pKa = 6.33 TGKK131 pKa = 10.46 VMKK134 pKa = 10.24 RR135 pKa = 11.84 FPADD139 pKa = 3.06 VFEE142 pKa = 4.31 KK143 pKa = 10.86 SKK145 pKa = 10.99 FIGMYY150 pKa = 9.95 DD151 pKa = 3.1 RR152 pKa = 11.84 HH153 pKa = 5.91 LTTLRR158 pKa = 11.84 EE159 pKa = 3.95 KK160 pKa = 10.7 ACCKK164 pKa = 10.22 KK165 pKa = 10.24 EE166 pKa = 3.84 RR167 pKa = 11.84 NQIQSKK173 pKa = 10.16 LIQLRR178 pKa = 11.84 QLKK181 pKa = 9.55 PSCDD185 pKa = 3.61 FLAGTVSGVPGSGKK199 pKa = 8.62 STLLKK204 pKa = 10.58 NVQKK208 pKa = 10.71 KK209 pKa = 10.06 LKK211 pKa = 10.82 NSVCLLANKK220 pKa = 8.26 EE221 pKa = 4.19 LKK223 pKa = 10.68 GDD225 pKa = 3.81 FAGVPSVFSVEE236 pKa = 4.04 EE237 pKa = 4.03 MLLSAVPSSFNVMLVDD253 pKa = 5.45 EE254 pKa = 4.56 YY255 pKa = 11.33 TLTQSAEE262 pKa = 3.72 ILLLQRR268 pKa = 11.84 KK269 pKa = 8.82 LGAKK273 pKa = 9.47 IVVLFGDD280 pKa = 4.1 RR281 pKa = 11.84 EE282 pKa = 4.06 QGNTNKK288 pKa = 8.89 LTSPEE293 pKa = 4.0 WLHH296 pKa = 6.05 VPIVFSSDD304 pKa = 2.66 SSHH307 pKa = 7.14 RR308 pKa = 11.84 FGPEE312 pKa = 3.2 TAKK315 pKa = 10.67 FCEE318 pKa = 4.21 DD319 pKa = 2.9 QGFSLEE325 pKa = 4.13 GRR327 pKa = 11.84 GGEE330 pKa = 4.08 DD331 pKa = 3.63 KK332 pKa = 10.58 IVKK335 pKa = 9.85 GDD337 pKa = 3.68 YY338 pKa = 10.18 EE339 pKa = 4.88 GEE341 pKa = 4.42 GEE343 pKa = 4.09 DD344 pKa = 4.72 TEE346 pKa = 5.19 VNLCFTEE353 pKa = 4.21 EE354 pKa = 4.01 TKK356 pKa = 11.12 ADD358 pKa = 3.73 LAEE361 pKa = 4.27 VQVEE365 pKa = 4.18 AFLVSSVQGRR375 pKa = 11.84 TFSSVSLFVRR385 pKa = 11.84 EE386 pKa = 3.41 NDD388 pKa = 3.37 KK389 pKa = 11.1 PVFSDD394 pKa = 2.82 PHH396 pKa = 6.94 LRR398 pKa = 11.84 LVAITRR404 pKa = 11.84 HH405 pKa = 5.75 RR406 pKa = 11.84 KK407 pKa = 9.08 LLSIRR412 pKa = 11.84 ADD414 pKa = 3.39 PEE416 pKa = 4.05 VWVSFMFATRR426 pKa = 11.84 EE427 pKa = 4.1 GEE429 pKa = 4.26 EE430 pKa = 4.35 VDD432 pKa = 3.35 THH434 pKa = 7.01 CYY436 pKa = 10.33 GEE438 pKa = 3.99 EE439 pKa = 3.93 HH440 pKa = 6.94 RR441 pKa = 11.84 PDD443 pKa = 3.21 EE444 pKa = 4.85 AEE446 pKa = 3.76
Molecular weight: 51.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.37
IPC2_protein 6.376
IPC_protein 6.465
Toseland 6.59
ProMoST 6.664
Dawson 6.839
Bjellqvist 6.795
Wikipedia 6.839
Rodwell 6.839
Grimsley 6.693
Solomon 6.854
Lehninger 6.854
Nozaki 7.102
DTASelect 7.22
Thurlkill 7.249
EMBOSS 7.234
Sillero 7.234
Patrickios 4.762
IPC_peptide 6.868
IPC2_peptide 6.81
IPC2.peptide.svr19 6.831
Protein with the highest isoelectric point:
>sp|Q08316|P39_PCV87 Protein P39 OS=Peanut clump virus (isolate 87/TGTA2) OX=652837 PE=4 SV=1
MM1 pKa = 7.21 VKK3 pKa = 9.07 STVPTRR9 pKa = 11.84 PNKK12 pKa = 9.03 YY13 pKa = 7.94 WPGVVAIGLVSLFIFLSVSNQKK35 pKa = 10.42 HH36 pKa = 4.66 STTSGDD42 pKa = 3.85 NIHH45 pKa = 6.69 KK46 pKa = 9.94 FSNGGTYY53 pKa = 9.73 RR54 pKa = 11.84 DD55 pKa = 3.52 GSKK58 pKa = 10.71 CITYY62 pKa = 10.28 NRR64 pKa = 11.84 NSPLAYY70 pKa = 10.26 NGSSSNNTLFWLCLLGLSMVWIAYY94 pKa = 9.43 CGYY97 pKa = 10.69 KK98 pKa = 10.11 SLSGQWHH105 pKa = 6.19 SCQHH109 pKa = 6.59 DD110 pKa = 3.64 KK111 pKa = 11.6 NEE113 pKa = 4.19 RR114 pKa = 11.84 NFLFEE119 pKa = 4.32 CFEE122 pKa = 4.15
Molecular weight: 13.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.432
IPC2_protein 8.214
IPC_protein 8.185
Toseland 8.053
ProMoST 8.58
Dawson 8.799
Bjellqvist 9.063
Wikipedia 8.77
Rodwell 8.829
Grimsley 7.849
Solomon 8.99
Lehninger 8.99
Nozaki 9.238
DTASelect 8.814
Thurlkill 8.858
EMBOSS 8.99
Sillero 9.165
Patrickios 3.427
IPC_peptide 8.99
IPC2_peptide 8.185
IPC2.peptide.svr19 8.135
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3091
122
1672
441.6
50.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.597 ± 0.36
2.297 ± 0.357
5.791 ± 0.319
7.409 ± 0.829
4.756 ± 0.215
5.532 ± 0.574
1.973 ± 0.236
4.109 ± 0.419
7.667 ± 0.646
9.803 ± 0.282
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.168 ± 0.249
4.012 ± 0.432
3.785 ± 0.237
3.429 ± 0.506
5.985 ± 0.591
7.894 ± 0.5
4.562 ± 0.37
8.153 ± 0.293
1.326 ± 0.21
3.72 ± 0.567
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here