Snodgrassella alvi wkB2
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2295 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X2HDL6|X2HDL6_9NEIS Uncharacterized protein OS=Snodgrassella alvi wkB2 OX=1196094 GN=SALWKB2_1901 PE=4 SV=1
MM1 pKa = 7.12 EE2 pKa = 5.06 TLNNLYY8 pKa = 10.02 RR9 pKa = 11.84 QQNNSQPRR17 pKa = 11.84 TEE19 pKa = 4.08 INSLKK24 pKa = 10.44 SAAFAEE30 pKa = 4.89 SPNSANTNGLIQGNDD45 pKa = 3.19 WVKK48 pKa = 10.98 DD49 pKa = 3.55 RR50 pKa = 11.84 YY51 pKa = 10.14 VFRR54 pKa = 11.84 NGHH57 pKa = 4.66 GQHH60 pKa = 6.62 TISDD64 pKa = 3.63 QGYY67 pKa = 9.61 DD68 pKa = 3.19 SSDD71 pKa = 3.81 SYY73 pKa = 10.6 WQQQRR78 pKa = 11.84 NDD80 pKa = 3.11 IVFEE84 pKa = 4.54 NINSAEE90 pKa = 4.0 ALFKK94 pKa = 10.89 RR95 pKa = 11.84 SGNNLIIYY103 pKa = 7.24 TNNSRR108 pKa = 11.84 DD109 pKa = 3.81 SVILNDD115 pKa = 3.42 YY116 pKa = 10.79 FDD118 pKa = 4.0 YY119 pKa = 11.2 NKK121 pKa = 10.52 NSRR124 pKa = 11.84 AFNFIFNDD132 pKa = 3.3 KK133 pKa = 10.2 TITYY137 pKa = 8.43 EE138 pKa = 4.02 NLKK141 pKa = 10.28 SDD143 pKa = 3.52 YY144 pKa = 9.23 TFSVNGDD151 pKa = 3.59 YY152 pKa = 11.09 NDD154 pKa = 4.19 NIINGWQGKK163 pKa = 9.36 DD164 pKa = 3.23 LLYY167 pKa = 11.18 GGDD170 pKa = 4.7 GNDD173 pKa = 3.65 TLNGNDD179 pKa = 5.02 GDD181 pKa = 3.96 DD182 pKa = 3.66 TLYY185 pKa = 11.47 GEE187 pKa = 5.09 AGNDD191 pKa = 3.1 ILNGGNGNDD200 pKa = 3.55 TLYY203 pKa = 11.24 GGTGNDD209 pKa = 3.79 TLNGADD215 pKa = 4.52 GDD217 pKa = 4.6 DD218 pKa = 5.13 IINGDD223 pKa = 4.24 DD224 pKa = 4.55 GDD226 pKa = 5.21 DD227 pKa = 3.53 ILYY230 pKa = 10.88 GDD232 pKa = 5.22 AGNDD236 pKa = 3.55 QLNGGYY242 pKa = 10.97 GNDD245 pKa = 3.81 TLNGGNGNDD254 pKa = 3.8 ILNGDD259 pKa = 4.01 GGDD262 pKa = 4.31 DD263 pKa = 3.69 ILYY266 pKa = 10.61 GDD268 pKa = 5.08 AGNDD272 pKa = 3.61 VLNGGYY278 pKa = 10.86 GNDD281 pKa = 4.14 TITGGTGFDD290 pKa = 4.04 ILNGGYY296 pKa = 10.9 GNDD299 pKa = 3.28 ILNGGDD305 pKa = 3.75 YY306 pKa = 11.1 EE307 pKa = 4.22 KK308 pKa = 10.93 DD309 pKa = 2.98 QYY311 pKa = 11.2 IFQRR315 pKa = 11.84 GHH317 pKa = 5.47 GQDD320 pKa = 3.69 VINDD324 pKa = 3.7 KK325 pKa = 11.36 GDD327 pKa = 3.88 DD328 pKa = 3.73 YY329 pKa = 11.53 AISYY333 pKa = 7.58 LQQKK337 pKa = 10.42 RR338 pKa = 11.84 NDD340 pKa = 3.71 LVFQGANSVNALFTRR355 pKa = 11.84 AGNDD359 pKa = 3.65 LIIRR363 pKa = 11.84 AYY365 pKa = 9.03 GTTDD369 pKa = 3.07 SVTLPDD375 pKa = 4.24 YY376 pKa = 11.2 FDD378 pKa = 4.75 LNKK381 pKa = 10.34 SSRR384 pKa = 11.84 AFNFIFDD391 pKa = 4.8 DD392 pKa = 3.66 KK393 pKa = 11.16 TITYY397 pKa = 8.89 EE398 pKa = 4.41 NLKK401 pKa = 8.68 TDD403 pKa = 3.34 YY404 pKa = 10.79 GIPSNGDD411 pKa = 2.97 AGNNTLIGWVGKK423 pKa = 10.28 DD424 pKa = 3.43 IMNGGYY430 pKa = 10.75 GNDD433 pKa = 3.7 VLWGRR438 pKa = 11.84 NGDD441 pKa = 4.35 DD442 pKa = 3.8 ILNGGDD448 pKa = 5.22 GNDD451 pKa = 3.82 EE452 pKa = 4.38 LNGEE456 pKa = 4.24 NGDD459 pKa = 4.7 DD460 pKa = 4.51 ILNGDD465 pKa = 4.35 YY466 pKa = 11.35 GCDD469 pKa = 3.51 TLNGGAGNDD478 pKa = 3.46 ILNGGNFEE486 pKa = 4.02 KK487 pKa = 10.63 DD488 pKa = 2.42 RR489 pKa = 11.84 YY490 pKa = 10.28 LFQKK494 pKa = 10.67 GHH496 pKa = 5.81 GQDD499 pKa = 4.18 IINDD503 pKa = 3.56 MSKK506 pKa = 10.66 PNQTSAINDD515 pKa = 3.75 LVFQGAKK522 pKa = 10.05 SADD525 pKa = 3.33 AVFTRR530 pKa = 11.84 SGNNLVISAYY540 pKa = 10.29 GSNDD544 pKa = 3.07 SVTLIDD550 pKa = 3.82 YY551 pKa = 10.31 FDD553 pKa = 5.25 YY554 pKa = 11.09 ILGSGANSKK563 pKa = 9.98 IDD565 pKa = 3.43 STLDD569 pKa = 3.07 SRR571 pKa = 11.84 AFNFIFDD578 pKa = 4.56 DD579 pKa = 3.61 RR580 pKa = 11.84 TITYY584 pKa = 10.05 EE585 pKa = 3.96 YY586 pKa = 10.36 FKK588 pKa = 11.49 NNYY591 pKa = 7.78 TFTANGNDD599 pKa = 3.31 QNNIIRR605 pKa = 11.84 GWEE608 pKa = 3.97 GKK610 pKa = 10.26 NILNGGAGNDD620 pKa = 3.57 TLYY623 pKa = 11.43 GDD625 pKa = 4.6 GSNDD629 pKa = 2.92 ILIGGTGNDD638 pKa = 3.02 ILYY641 pKa = 10.82 GGGGQKK647 pKa = 10.67 DD648 pKa = 2.73 IFQFEE653 pKa = 4.49 KK654 pKa = 10.99 GHH656 pKa = 5.53 GQDD659 pKa = 3.61 VIYY662 pKa = 11.06 NGYY665 pKa = 6.88 TTDD668 pKa = 3.48 NTANSYY674 pKa = 11.44 DD675 pKa = 4.5 LIFKK679 pKa = 9.66 VAYY682 pKa = 9.01 SANAAFSRR690 pKa = 11.84 YY691 pKa = 8.89 GDD693 pKa = 3.44 NLIIHH698 pKa = 7.64 AYY700 pKa = 10.19 GSDD703 pKa = 3.55 DD704 pKa = 3.57 SVTIINYY711 pKa = 7.39 FNCSNKK717 pKa = 9.52 QDD719 pKa = 3.62 FNFIFDD725 pKa = 4.7 DD726 pKa = 3.53 KK727 pKa = 10.59 TINYY731 pKa = 9.81 QYY733 pKa = 10.9 ILNNFPLTFIGDD745 pKa = 3.46 SRR747 pKa = 11.84 RR748 pKa = 11.84 NYY750 pKa = 8.86 EE751 pKa = 4.27 NGWEE755 pKa = 4.41 GKK757 pKa = 10.2 NILNGGGGNDD767 pKa = 3.0 VLYY770 pKa = 10.98 GNKK773 pKa = 9.41 QDD775 pKa = 6.06 DD776 pKa = 4.31 ILNGDD781 pKa = 4.61 DD782 pKa = 4.03 GDD784 pKa = 5.23 DD785 pKa = 3.79 YY786 pKa = 11.95 LSGQDD791 pKa = 5.61 GNDD794 pKa = 3.59 TLNGGAGNDD803 pKa = 3.68 RR804 pKa = 11.84 LNGGNGSDD812 pKa = 3.85 ILNGGAGNDD821 pKa = 3.61 ILDD824 pKa = 4.14 GGHH827 pKa = 6.06 NEE829 pKa = 3.53 KK830 pKa = 9.53 DD831 pKa = 3.11 TYY833 pKa = 10.56 IFQRR837 pKa = 11.84 GHH839 pKa = 5.64 GQDD842 pKa = 4.02 IIQEE846 pKa = 4.44 SYY848 pKa = 11.06 YY849 pKa = 10.77 SNQSVEE855 pKa = 4.18 VNDD858 pKa = 4.18 VIFEE862 pKa = 4.26 GVNFANAVFTRR873 pKa = 11.84 SDD875 pKa = 3.49 NNLVIHH881 pKa = 7.28 AYY883 pKa = 10.39 GSDD886 pKa = 3.62 DD887 pKa = 3.74 SVTINNYY894 pKa = 9.37 FYY896 pKa = 11.27 KK897 pKa = 10.63 NSYY900 pKa = 9.49 LPNSNSHH907 pKa = 6.78 AYY909 pKa = 10.61 NFIFNDD915 pKa = 4.01 KK916 pKa = 10.27 IITYY920 pKa = 10.52 SDD922 pKa = 3.85 LVNNDD927 pKa = 3.33 YY928 pKa = 11.37 SFTAIGDD935 pKa = 3.65 NTNNKK940 pKa = 8.71 IDD942 pKa = 3.45 GWDD945 pKa = 3.56 GKK947 pKa = 10.6 NILSGGGGNDD957 pKa = 3.38 FLYY960 pKa = 10.66 GQDD963 pKa = 4.57 KK964 pKa = 11.33 DD965 pKa = 4.85 DD966 pKa = 4.47 VLNGDD971 pKa = 5.08 DD972 pKa = 6.4 GDD974 pKa = 5.13 DD975 pKa = 3.85 EE976 pKa = 5.83 LNGAGGDD983 pKa = 4.14 DD984 pKa = 3.89 ILNGGDD990 pKa = 4.8 GDD992 pKa = 4.13 DD993 pKa = 4.0 KK994 pKa = 11.62 LYY996 pKa = 10.95 GGKK999 pKa = 10.64 GSDD1002 pKa = 3.5 VLIGGTGNDD1011 pKa = 3.9 ILNGGYY1017 pKa = 8.88 NEE1019 pKa = 4.22 KK1020 pKa = 9.51 DD1021 pKa = 2.99 TYY1023 pKa = 10.87 LFQYY1027 pKa = 10.74 GHH1029 pKa = 6.27 GQDD1032 pKa = 4.8 IINEE1036 pKa = 4.62 GYY1038 pKa = 10.42 NGNQPVYY1045 pKa = 9.78 ANDD1048 pKa = 3.72 VIFEE1052 pKa = 4.2 DD1053 pKa = 5.31 AYY1055 pKa = 11.76 SNDD1058 pKa = 3.12 ATFTRR1063 pKa = 11.84 SGNDD1067 pKa = 3.5 LVIHH1071 pKa = 7.32 AYY1073 pKa = 10.43 HH1074 pKa = 6.82 SDD1076 pKa = 3.69 DD1077 pKa = 4.79 SVTLTNYY1084 pKa = 7.71 FTDD1087 pKa = 4.08 GSKK1090 pKa = 10.48 RR1091 pKa = 11.84 AFNFIFTDD1099 pKa = 3.23 KK1100 pKa = 10.77 TIDD1103 pKa = 3.68 YY1104 pKa = 9.2 EE1105 pKa = 4.3 YY1106 pKa = 11.04 LKK1108 pKa = 11.07 NNYY1111 pKa = 7.84 TFTPTVITNKK1121 pKa = 9.67 TIVGTDD1127 pKa = 3.1 SNDD1130 pKa = 3.1 ILNGGVGNDD1139 pKa = 3.48 TLYY1142 pKa = 11.4 GLSGNDD1148 pKa = 3.47 TLNGNDD1154 pKa = 4.36 GSDD1157 pKa = 3.75 ILWGGYY1163 pKa = 10.56 DD1164 pKa = 3.73 NDD1166 pKa = 4.35 ILNGGNGNDD1175 pKa = 3.72 TLYY1178 pKa = 11.21 GDD1180 pKa = 3.9 SGYY1183 pKa = 11.02 DD1184 pKa = 3.25 ILNGGAGNDD1193 pKa = 3.38 ILNGGDD1199 pKa = 3.6 RR1200 pKa = 11.84 EE1201 pKa = 3.89 KK1202 pKa = 11.23 DD1203 pKa = 2.78 RR1204 pKa = 11.84 YY1205 pKa = 10.37 LFQRR1209 pKa = 11.84 GHH1211 pKa = 5.91 GQDD1214 pKa = 3.77 TINDD1218 pKa = 3.6 TGYY1221 pKa = 10.31 YY1222 pKa = 9.73 NYY1224 pKa = 10.78 SNEE1227 pKa = 3.54 VDD1229 pKa = 4.64 DD1230 pKa = 5.98 IVFEE1234 pKa = 4.35 GANFADD1240 pKa = 3.49 AVFIRR1245 pKa = 11.84 SGKK1248 pKa = 9.76 DD1249 pKa = 3.08 LIVRR1253 pKa = 11.84 AYY1255 pKa = 10.24 GSEE1258 pKa = 4.04 DD1259 pKa = 3.72 SVTLTDD1265 pKa = 3.73 YY1266 pKa = 7.59 FTNGLANAFNFIFNDD1281 pKa = 3.28 KK1282 pKa = 10.87 SIDD1285 pKa = 3.46 YY1286 pKa = 10.62 QYY1288 pKa = 11.1 IKK1290 pKa = 11.45 NNFTFTADD1298 pKa = 4.94 GDD1300 pKa = 4.22 DD1301 pKa = 3.67 TDD1303 pKa = 3.68 NTINGWRR1310 pKa = 11.84 DD1311 pKa = 2.86 GKK1313 pKa = 10.73 NILNGGAGNDD1323 pKa = 3.41 ILNGEE1328 pKa = 4.71 DD1329 pKa = 4.93 KK1330 pKa = 11.28 DD1331 pKa = 4.53 DD1332 pKa = 3.85 VLNGGDD1338 pKa = 4.62 GNDD1341 pKa = 3.31 SLYY1344 pKa = 11.14 GNNGIDD1350 pKa = 3.74 TLNGGAGNDD1359 pKa = 3.81 FLDD1362 pKa = 4.88 GGDD1365 pKa = 3.49 SADD1368 pKa = 3.41 RR1369 pKa = 11.84 YY1370 pKa = 10.35 LFQRR1374 pKa = 11.84 GHH1376 pKa = 5.85 GQDD1379 pKa = 3.51 IIDD1382 pKa = 4.52 DD1383 pKa = 4.04 KK1384 pKa = 11.53 FLTSDD1389 pKa = 3.3 VNEE1392 pKa = 4.31 VVFQGAKK1399 pKa = 9.82 FADD1402 pKa = 3.63 AVFTSSGEE1410 pKa = 3.78 NLIIHH1415 pKa = 7.5 AYY1417 pKa = 9.52 GSNDD1421 pKa = 3.02 SVTINDD1427 pKa = 3.38 YY1428 pKa = 11.01 FYY1430 pKa = 10.7 IDD1432 pKa = 3.4 SYY1434 pKa = 11.75 KK1435 pKa = 9.78 PAKK1438 pKa = 9.7 AYY1440 pKa = 9.95 KK1441 pKa = 10.17 FIFEE1445 pKa = 4.78 DD1446 pKa = 5.48 RR1447 pKa = 11.84 IITYY1451 pKa = 10.35 QDD1453 pKa = 2.97 FQNYY1457 pKa = 6.05 YY1458 pKa = 9.91 TYY1460 pKa = 9.07 ITHH1463 pKa = 7.24 GDD1465 pKa = 3.54 SSNDD1469 pKa = 3.6 TINGINGWEE1478 pKa = 4.24 GKK1480 pKa = 10.29 NIISGGYY1487 pKa = 10.51 GNDD1490 pKa = 3.33 KK1491 pKa = 10.97 LYY1493 pKa = 10.96 GQNKK1497 pKa = 10.02 DD1498 pKa = 4.66 DD1499 pKa = 5.0 ILNGDD1504 pKa = 4.5 DD1505 pKa = 5.81 GDD1507 pKa = 4.24 DD1508 pKa = 4.08 TLHH1511 pKa = 6.24 GWSGNDD1517 pKa = 3.25 ILNGGNGDD1525 pKa = 3.9 DD1526 pKa = 4.09 YY1527 pKa = 11.6 LYY1529 pKa = 11.34 GDD1531 pKa = 4.32 GGFDD1535 pKa = 3.26 ILNGGTGNDD1544 pKa = 3.74 YY1545 pKa = 11.32 LDD1547 pKa = 3.85 GGDD1550 pKa = 3.57 RR1551 pKa = 11.84 KK1552 pKa = 10.57 DD1553 pKa = 3.27 RR1554 pKa = 11.84 YY1555 pKa = 10.37 LFQRR1559 pKa = 11.84 GHH1561 pKa = 5.85 GQDD1564 pKa = 3.7 IIDD1567 pKa = 4.87 DD1568 pKa = 4.06 RR1569 pKa = 11.84 GNDD1572 pKa = 3.03 ISSEE1576 pKa = 4.02 YY1577 pKa = 10.57 NDD1579 pKa = 3.32 IVFEE1583 pKa = 4.29 GTNLTNAVFSRR1594 pKa = 11.84 SGYY1597 pKa = 9.91 DD1598 pKa = 3.41 LIVRR1602 pKa = 11.84 AYY1604 pKa = 8.87 GTDD1607 pKa = 3.43 DD1608 pKa = 3.48 SVTLSRR1614 pKa = 11.84 YY1615 pKa = 9.04 FDD1617 pKa = 3.49 SSARR1621 pKa = 11.84 AHH1623 pKa = 6.05 SFNFIFDD1630 pKa = 4.45 DD1631 pKa = 3.71 KK1632 pKa = 10.61 TISYY1636 pKa = 10.84 LDD1638 pKa = 3.88 IIKK1641 pKa = 10.67 GNYY1644 pKa = 9.54 LSAQTGKK1651 pKa = 9.94 SGYY1654 pKa = 10.45 GITSKK1659 pKa = 11.13 SLLNRR1664 pKa = 11.84 DD1665 pKa = 3.47 LLTRR1669 pKa = 11.84 NSTPVANEE1677 pKa = 3.86 SPAVASQAQQLASAMSGFTARR1698 pKa = 11.84 QDD1700 pKa = 3.36 SSLSGMPDD1708 pKa = 3.24 QIQQFVQQNNFAAYY1722 pKa = 8.44 WGNN1725 pKa = 3.28
Molecular weight: 188.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.821
IPC_protein 3.897
Toseland 3.643
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.884
Rodwell 3.706
Grimsley 3.541
Solomon 3.91
Lehninger 3.872
Nozaki 4.012
DTASelect 4.355
Thurlkill 3.706
EMBOSS 3.884
Sillero 4.024
Patrickios 1.545
IPC_peptide 3.897
IPC2_peptide 3.999
IPC2.peptide.svr19 3.9
Protein with the highest isoelectric point:
>tr|X2H7L0|X2H7L0_9NEIS Isochorismate synthase OS=Snodgrassella alvi wkB2 OX=1196094 GN=SALWKB2_2022 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.43 RR14 pKa = 11.84 THH16 pKa = 5.89 GFLVRR21 pKa = 11.84 SKK23 pKa = 9.38 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2295
0
2295
695041
37
3592
302.9
33.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.999 ± 0.073
1.106 ± 0.023
5.17 ± 0.059
5.339 ± 0.066
3.93 ± 0.052
6.731 ± 0.07
2.343 ± 0.027
6.979 ± 0.056
5.139 ± 0.051
10.47 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.473 ± 0.035
4.962 ± 0.081
3.962 ± 0.048
5.197 ± 0.052
4.727 ± 0.052
6.091 ± 0.099
5.412 ± 0.061
6.343 ± 0.05
1.318 ± 0.022
3.312 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here