Thelohanellus kitauei (Myxosporean)
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14792 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C2N5D8|A0A0C2N5D8_THEKT Integrase catalytic domain-containing protein OS=Thelohanellus kitauei OX=669202 GN=RF11_02980 PE=4 SV=1
MM1 pKa = 7.36 GCEE4 pKa = 3.71 KK5 pKa = 10.91 SSFSVYY11 pKa = 10.63 NSNHH15 pKa = 5.19 LHH17 pKa = 5.94 LTQNTTEE24 pKa = 4.21 PWVEE28 pKa = 3.57 MLYY31 pKa = 10.64 NCFDD35 pKa = 3.72 RR36 pKa = 11.84 VLKK39 pKa = 11.14 SEE41 pKa = 5.05 DD42 pKa = 3.47 DD43 pKa = 4.01 DD44 pKa = 4.3 NRR46 pKa = 11.84 TLSEE50 pKa = 4.15 CCQVNGLKK58 pKa = 9.87 TGIYY62 pKa = 9.86 RR63 pKa = 11.84 FSDD66 pKa = 3.51 DD67 pKa = 5.84 CLDD70 pKa = 4.16 CVCSQTNCLAAPRR83 pKa = 11.84 MHH85 pKa = 6.53 EE86 pKa = 4.02 VSDD89 pKa = 4.06 FLDD92 pKa = 3.75 DD93 pKa = 3.59 MGISFFDD100 pKa = 3.45 TTGDD104 pKa = 3.43 DD105 pKa = 3.7 VFFNDD110 pKa = 4.0 TEE112 pKa = 4.24 NMAEE116 pKa = 4.08 ANFVIDD122 pKa = 5.73 DD123 pKa = 4.06 EE124 pKa = 6.13 SDD126 pKa = 3.57 DD127 pKa = 4.22 EE128 pKa = 4.74 EE129 pKa = 5.27 GSKK132 pKa = 11.26 SNLL135 pKa = 3.32
Molecular weight: 15.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.846
IPC_protein 3.846
Toseland 3.63
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.541
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.19
Thurlkill 3.681
EMBOSS 3.783
Sillero 3.961
Patrickios 0.769
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A0C2JJY2|A0A0C2JJY2_THEKT Uncharacterized protein OS=Thelohanellus kitauei OX=669202 GN=RF11_07295 PE=4 SV=1
MM1 pKa = 7.72 AEE3 pKa = 4.01 YY4 pKa = 10.73 SPGGRR9 pKa = 11.84 IRR11 pKa = 11.84 VTLKK15 pKa = 10.25 IRR17 pKa = 11.84 DD18 pKa = 4.2 PPTEE22 pKa = 4.07 TLPALRR28 pKa = 11.84 TDD30 pKa = 3.31 VLMDD34 pKa = 4.24 PKK36 pKa = 10.6 LRR38 pKa = 11.84 RR39 pKa = 11.84 SQRR42 pKa = 11.84 EE43 pKa = 3.46 RR44 pKa = 11.84 GRR46 pKa = 11.84 EE47 pKa = 3.38 QSFRR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 SRR55 pKa = 11.84 RR56 pKa = 11.84 APVKK60 pKa = 9.43 GTALFTTTGPSTLCPDD76 pKa = 4.47 LDD78 pKa = 4.01 SASIEE83 pKa = 4.37 CIGVSFGVPRR93 pKa = 11.84 FAYY96 pKa = 9.44 PSALGSICSSSVIFTIKK113 pKa = 8.84 GTKK116 pKa = 8.82 NQATT120 pKa = 3.8
Molecular weight: 13.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.56
IPC_protein 10.482
Toseland 10.76
ProMoST 10.526
Dawson 10.818
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 10.935
Grimsley 10.862
Solomon 11.008
Lehninger 10.965
Nozaki 10.76
DTASelect 10.57
Thurlkill 10.745
EMBOSS 11.169
Sillero 10.774
Patrickios 10.701
IPC_peptide 11.008
IPC2_peptide 9.838
IPC2.peptide.svr19 8.656
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14792
0
14792
3533531
99
4546
238.9
27.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.342 ± 0.018
2.205 ± 0.012
5.539 ± 0.024
6.119 ± 0.031
5.306 ± 0.018
4.323 ± 0.019
2.594 ± 0.011
7.909 ± 0.021
7.135 ± 0.024
9.166 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.472 ± 0.009
5.86 ± 0.018
4.031 ± 0.017
3.654 ± 0.015
4.972 ± 0.017
8.249 ± 0.024
5.503 ± 0.021
5.663 ± 0.016
0.973 ± 0.006
3.985 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here