Amino acid dipepetide frequency for Acidocella aminolytica 101 = DSM 11237

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.236AlaAla: 18.236 ± 0.202	1.201AlaCys: 1.201 ± 0.035	5.93AlaAsp: 5.93 ± 0.081	7.675AlaGlu: 7.675 ± 0.098	4.206AlaPhe: 4.206 ± 0.071	11.015AlaGly: 11.015 ± 0.112	2.483AlaHis: 2.483 ± 0.048	6.01AlaIle: 6.01 ± 0.081	4.617AlaLys: 4.617 ± 0.078	14.989AlaLeu: 14.989 ± 0.172	3.637AlaMet: 3.637 ± 0.052	3.181AlaAsn: 3.181 ± 0.056	6.086AlaPro: 6.086 ± 0.091	4.853AlaGln: 4.853 ± 0.084	8.312AlaArg: 8.312 ± 0.113	6.358AlaSer: 6.358 ± 0.086	5.955AlaThr: 5.955 ± 0.072	8.824AlaVal: 8.824 ± 0.093	1.625AlaTrp: 1.625 ± 0.042	2.665AlaTyr: 2.665 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	1.179CysAla: 1.179 ± 0.034	0.114CysCys: 0.114 ± 0.01	0.49CysAsp: 0.49 ± 0.023	0.463CysGlu: 0.463 ± 0.019	0.366CysPhe: 0.366 ± 0.018	0.992CysGly: 0.992 ± 0.031	0.277CysHis: 0.277 ± 0.017	0.432CysIle: 0.432 ± 0.02	0.222CysLys: 0.222 ± 0.013	0.952CysLeu: 0.952 ± 0.031	0.189CysMet: 0.189 ± 0.015	0.243CysAsn: 0.243 ± 0.014	0.524CysPro: 0.524 ± 0.02	0.256CysGln: 0.256 ± 0.016	0.599CysArg: 0.599 ± 0.024	0.467CysSer: 0.467 ± 0.019	0.502CysThr: 0.502 ± 0.02	0.651CysVal: 0.651 ± 0.026	0.139CysTrp: 0.139 ± 0.012	0.201CysTyr: 0.201 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	5.89AspAla: 5.89 ± 0.076	0.419AspCys: 0.419 ± 0.021	2.313AspAsp: 2.313 ± 0.054	3.191AspGlu: 3.191 ± 0.059	1.978AspPhe: 1.978 ± 0.044	4.354AspGly: 4.354 ± 0.074	1.093AspHis: 1.093 ± 0.032	2.806AspIle: 2.806 ± 0.046	1.645AspLys: 1.645 ± 0.042	5.08AspLeu: 5.08 ± 0.073	1.306AspMet: 1.306 ± 0.036	1.262AspAsn: 1.262 ± 0.034	2.87AspPro: 2.87 ± 0.049	1.536AspGln: 1.536 ± 0.041	2.953AspArg: 2.953 ± 0.061	2.094AspSer: 2.094 ± 0.044	2.291AspThr: 2.291 ± 0.05	3.687AspVal: 3.687 ± 0.059	0.903AspTrp: 0.903 ± 0.027	1.377AspTyr: 1.377 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	7.976GluAla: 7.976 ± 0.113	0.329GluCys: 0.329 ± 0.018	2.572GluAsp: 2.572 ± 0.051	2.98GluGlu: 2.98 ± 0.069	1.8GluPhe: 1.8 ± 0.041	3.979GluGly: 3.979 ± 0.057	1.267GluHis: 1.267 ± 0.033	3.464GluIle: 3.464 ± 0.064	2.116GluLys: 2.116 ± 0.054	5.104GluLeu: 5.104 ± 0.074	1.592GluMet: 1.592 ± 0.042	1.598GluAsn: 1.598 ± 0.037	2.243GluPro: 2.243 ± 0.042	2.218GluGln: 2.218 ± 0.052	4.095GluArg: 4.095 ± 0.078	2.29GluSer: 2.29 ± 0.052	3.534GluThr: 3.534 ± 0.055	3.54GluVal: 3.54 ± 0.06	0.595GluTrp: 0.595 ± 0.023	0.973GluTyr: 0.973 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	4.408PheAla: 4.408 ± 0.066	0.521PheCys: 0.521 ± 0.021	2.041PheAsp: 2.041 ± 0.045	1.914PheGlu: 1.914 ± 0.046	1.409PhePhe: 1.409 ± 0.044	3.733PheGly: 3.733 ± 0.063	0.768PheHis: 0.768 ± 0.027	1.868PheIle: 1.868 ± 0.043	1.037PheLys: 1.037 ± 0.03	3.678PheLeu: 3.678 ± 0.065	0.871PheMet: 0.871 ± 0.026	1.214PheAsn: 1.214 ± 0.036	1.61PhePro: 1.61 ± 0.04	1.007PheGln: 1.007 ± 0.033	1.955PheArg: 1.955 ± 0.039	2.475PheSer: 2.475 ± 0.048	2.351PheThr: 2.351 ± 0.051	2.575PheVal: 2.575 ± 0.055	0.567PheTrp: 0.567 ± 0.025	0.947PheTyr: 0.947 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	9.75GlyAla: 9.75 ± 0.112	0.836GlyCys: 0.836 ± 0.028	3.709GlyAsp: 3.709 ± 0.061	4.212GlyGlu: 4.212 ± 0.058	3.812GlyPhe: 3.812 ± 0.059	7.443GlyGly: 7.443 ± 0.241	2.017GlyHis: 2.017 ± 0.045	4.686GlyIle: 4.686 ± 0.07	3.43GlyLys: 3.43 ± 0.067	9.446GlyLeu: 9.446 ± 0.106	2.471GlyMet: 2.471 ± 0.051	2.336GlyAsn: 2.336 ± 0.069	3.567GlyPro: 3.567 ± 0.063	3.336GlyGln: 3.336 ± 0.06	5.411GlyArg: 5.411 ± 0.077	4.205GlySer: 4.205 ± 0.094	4.775GlyThr: 4.775 ± 0.098	6.228GlyVal: 6.228 ± 0.079	1.462GlyTrp: 1.462 ± 0.035	2.313GlyTyr: 2.313 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	2.542HisAla: 2.542 ± 0.048	0.225HisCys: 0.225 ± 0.013	1.212HisAsp: 1.212 ± 0.028	1.19HisGlu: 1.19 ± 0.037	0.967HisPhe: 0.967 ± 0.029	2.178HisGly: 2.178 ± 0.037	0.648HisHis: 0.648 ± 0.025	1.115HisIle: 1.115 ± 0.034	0.719HisLys: 0.719 ± 0.026	2.245HisLeu: 2.245 ± 0.042	0.513HisMet: 0.513 ± 0.02	0.618HisAsn: 0.618 ± 0.023	1.384HisPro: 1.384 ± 0.039	0.655HisGln: 0.655 ± 0.024	1.321HisArg: 1.321 ± 0.036	1.026HisSer: 1.026 ± 0.028	0.891HisThr: 0.891 ± 0.029	1.605HisVal: 1.605 ± 0.037	0.376HisTrp: 0.376 ± 0.019	0.609HisTyr: 0.609 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.07IleAla: 7.07 ± 0.081	0.596IleCys: 0.596 ± 0.023	2.857IleAsp: 2.857 ± 0.049	3.148IleGlu: 3.148 ± 0.065	1.837IlePhe: 1.837 ± 0.044	4.845IleGly: 4.845 ± 0.082	0.983IleHis: 0.983 ± 0.027	2.717IleIle: 2.717 ± 0.053	1.557IleLys: 1.557 ± 0.04	4.85IleLeu: 4.85 ± 0.07	1.039IleMet: 1.039 ± 0.032	1.634IleAsn: 1.634 ± 0.039	2.456IlePro: 2.456 ± 0.042	1.426IleGln: 1.426 ± 0.04	3.036IleArg: 3.036 ± 0.055	3.312IleSer: 3.312 ± 0.053	3.161IleThr: 3.161 ± 0.053	3.616IleVal: 3.616 ± 0.057	0.643IleTrp: 0.643 ± 0.024	1.158IleTyr: 1.158 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	4.418LysAla: 4.418 ± 0.057	0.165LysCys: 0.165 ± 0.012	1.591LysAsp: 1.591 ± 0.041	1.61LysGlu: 1.61 ± 0.045	1.092LysPhe: 1.092 ± 0.031	2.421LysGly: 2.421 ± 0.048	0.713LysHis: 0.713 ± 0.027	2.071LysIle: 2.071 ± 0.046	1.277LysLys: 1.277 ± 0.039	4.149LysLeu: 4.149 ± 0.069	0.817LysMet: 0.817 ± 0.029	0.98LysAsn: 0.98 ± 0.034	2.288LysPro: 2.288 ± 0.051	1.237LysGln: 1.237 ± 0.032	2.368LysArg: 2.368 ± 0.052	1.861LysSer: 1.861 ± 0.045	1.982LysThr: 1.982 ± 0.046	2.25LysVal: 2.25 ± 0.044	0.331LysTrp: 0.331 ± 0.018	0.655LysTyr: 0.655 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	14.877LeuAla: 14.877 ± 0.152	1.112LeuCys: 1.112 ± 0.031	5.504LeuAsp: 5.504 ± 0.073	5.27LeuGlu: 5.27 ± 0.079	3.729LeuPhe: 3.729 ± 0.068	8.776LeuGly: 8.776 ± 0.094	2.359LeuHis: 2.359 ± 0.051	5.153LeuIle: 5.153 ± 0.083	3.707LeuLys: 3.707 ± 0.057	10.883LeuLeu: 10.883 ± 0.145	2.289LeuMet: 2.289 ± 0.048	3.199LeuAsn: 3.199 ± 0.058	6.58LeuPro: 6.58 ± 0.096	3.09LeuGln: 3.09 ± 0.061	7.407LeuArg: 7.407 ± 0.102	6.79LeuSer: 6.79 ± 0.079	6.238LeuThr: 6.238 ± 0.076	7.359LeuVal: 7.359 ± 0.106	1.205LeuTrp: 1.205 ± 0.036	2.284LeuTyr: 2.284 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	3.337MetAla: 3.337 ± 0.055	0.15MetCys: 0.15 ± 0.011	1.152MetAsp: 1.152 ± 0.034	1.198MetGlu: 1.198 ± 0.036	0.772MetPhe: 0.772 ± 0.026	1.806MetGly: 1.806 ± 0.045	0.474MetHis: 0.474 ± 0.021	1.305MetIle: 1.305 ± 0.036	0.991MetLys: 0.991 ± 0.033	2.824MetLeu: 2.824 ± 0.052	0.623MetMet: 0.623 ± 0.028	0.833MetAsn: 0.833 ± 0.028	1.513MetPro: 1.513 ± 0.037	0.976MetGln: 0.976 ± 0.029	1.769MetArg: 1.769 ± 0.04	1.629MetSer: 1.629 ± 0.036	1.574MetThr: 1.574 ± 0.036	1.76MetVal: 1.76 ± 0.045	0.187MetTrp: 0.187 ± 0.013	0.282MetTyr: 0.282 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.666AsnAla: 3.666 ± 0.057	0.305AsnCys: 0.305 ± 0.018	1.295AsnAsp: 1.295 ± 0.034	1.368AsnGlu: 1.368 ± 0.036	1.123AsnPhe: 1.123 ± 0.032	2.732AsnGly: 2.732 ± 0.072	0.604AsnHis: 0.604 ± 0.021	1.605AsnIle: 1.605 ± 0.039	0.86AsnLys: 0.86 ± 0.028	3.072AsnLeu: 3.072 ± 0.062	0.636AsnMet: 0.636 ± 0.024	0.9AsnAsn: 0.9 ± 0.036	1.974AsnPro: 1.974 ± 0.048	1.027AsnGln: 1.027 ± 0.031	1.674AsnArg: 1.674 ± 0.039	1.37AsnSer: 1.37 ± 0.043	1.452AsnThr: 1.452 ± 0.041	2.006AsnVal: 2.006 ± 0.044	0.455AsnTrp: 0.455 ± 0.018	0.76AsnTyr: 0.76 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	6.564ProAla: 6.564 ± 0.097	0.397ProCys: 0.397 ± 0.019	3.437ProAsp: 3.437 ± 0.061	3.772ProGlu: 3.772 ± 0.061	1.983ProPhe: 1.983 ± 0.043	5.086ProGly: 5.086 ± 0.08	1.278ProHis: 1.278 ± 0.032	2.129ProIle: 2.129 ± 0.046	1.677ProLys: 1.677 ± 0.035	5.188ProLeu: 5.188 ± 0.075	1.254ProMet: 1.254 ± 0.035	1.533ProAsn: 1.533 ± 0.037	3.109ProPro: 3.109 ± 0.073	1.803ProGln: 1.803 ± 0.044	2.813ProArg: 2.813 ± 0.058	2.886ProSer: 2.886 ± 0.061	2.483ProThr: 2.483 ± 0.05	4.323ProVal: 4.323 ± 0.063	0.72ProTrp: 0.72 ± 0.025	1.234ProTyr: 1.234 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	4.918GlnAla: 4.918 ± 0.09	0.228GlnCys: 0.228 ± 0.015	1.704GlnAsp: 1.704 ± 0.045	1.713GlnGlu: 1.713 ± 0.04	1.12GlnPhe: 1.12 ± 0.032	2.698GlnGly: 2.698 ± 0.051	0.772GlnHis: 0.772 ± 0.026	2.06GlnIle: 2.06 ± 0.045	1.281GlnLys: 1.281 ± 0.035	3.182GlnLeu: 3.182 ± 0.052	0.937GlnMet: 0.937 ± 0.028	1.18GlnAsn: 1.18 ± 0.036	1.897GlnPro: 1.897 ± 0.04	1.366GlnGln: 1.366 ± 0.049	2.261GlnArg: 2.261 ± 0.048	2.047GlnSer: 2.047 ± 0.051	2.032GlnThr: 2.032 ± 0.045	2.298GlnVal: 2.298 ± 0.046	0.38GlnTrp: 0.38 ± 0.018	0.695GlnTyr: 0.695 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	7.752ArgAla: 7.752 ± 0.104	0.561ArgCys: 0.561 ± 0.022	3.172ArgAsp: 3.172 ± 0.054	3.685ArgGlu: 3.685 ± 0.072	2.648ArgPhe: 2.648 ± 0.051	4.41ArgGly: 4.41 ± 0.059	1.756ArgHis: 1.756 ± 0.04	3.404ArgIle: 3.404 ± 0.059	2.183ArgLys: 2.183 ± 0.043	7.953ArgLeu: 7.953 ± 0.112	1.659ArgMet: 1.659 ± 0.04	1.833ArgAsn: 1.833 ± 0.042	3.186ArgPro: 3.186 ± 0.058	2.576ArgGln: 2.576 ± 0.054	4.977ArgArg: 4.977 ± 0.091	2.804ArgSer: 2.804 ± 0.048	2.906ArgThr: 2.906 ± 0.053	4.362ArgVal: 4.362 ± 0.068	0.841ArgTrp: 0.841 ± 0.026	1.601ArgTyr: 1.601 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	6.363SerAla: 6.363 ± 0.074	0.524SerCys: 0.524 ± 0.021	2.399SerAsp: 2.399 ± 0.051	2.461SerGlu: 2.461 ± 0.049	2.361SerPhe: 2.361 ± 0.047	5.457SerGly: 5.457 ± 0.102	1.111SerHis: 1.111 ± 0.036	2.749SerIle: 2.749 ± 0.053	1.573SerLys: 1.573 ± 0.038	5.838SerLeu: 5.838 ± 0.078	1.302SerMet: 1.302 ± 0.032	1.556SerAsn: 1.556 ± 0.049	2.873SerPro: 2.873 ± 0.056	1.738SerGln: 1.738 ± 0.044	3.336SerArg: 3.336 ± 0.054	3.031SerSer: 3.031 ± 0.069	2.851SerThr: 2.851 ± 0.068	3.849SerVal: 3.849 ± 0.064	0.871SerTrp: 0.871 ± 0.029	1.407SerTyr: 1.407 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	6.547ThrAla: 6.547 ± 0.082	0.456ThrCys: 0.456 ± 0.021	2.529ThrAsp: 2.529 ± 0.045	2.593ThrGlu: 2.593 ± 0.053	1.673ThrPhe: 1.673 ± 0.042	5.391ThrGly: 5.391 ± 0.09	1.165ThrHis: 1.165 ± 0.032	2.875ThrIle: 2.875 ± 0.062	1.68ThrLys: 1.68 ± 0.044	6.264ThrLeu: 6.264 ± 0.085	1.254ThrMet: 1.254 ± 0.034	1.492ThrAsn: 1.492 ± 0.042	3.631ThrPro: 3.631 ± 0.062	1.825ThrGln: 1.825 ± 0.04	3.163ThrArg: 3.163 ± 0.06	2.912ThrSer: 2.912 ± 0.06	2.841ThrThr: 2.841 ± 0.07	3.877ThrVal: 3.877 ± 0.069	0.618ThrTrp: 0.618 ± 0.024	1.17ThrTyr: 1.17 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	8.39ValAla: 8.39 ± 0.106	0.699ValCys: 0.699 ± 0.026	3.234ValAsp: 3.234 ± 0.055	4.018ValGlu: 4.018 ± 0.062	2.61ValPhe: 2.61 ± 0.047	5.109ValGly: 5.109 ± 0.083	1.32ValHis: 1.32 ± 0.033	3.849ValIle: 3.849 ± 0.065	2.55ValLys: 2.55 ± 0.048	7.984ValLeu: 7.984 ± 0.088	1.948ValMet: 1.948 ± 0.047	2.162ValAsn: 2.162 ± 0.05	3.976ValPro: 3.976 ± 0.075	2.4ValGln: 2.4 ± 0.046	4.134ValArg: 4.134 ± 0.066	4.195ValSer: 4.195 ± 0.06	4.391ValThr: 4.391 ± 0.077	5.418ValVal: 5.418 ± 0.074	0.942ValTrp: 0.942 ± 0.032	1.38ValTyr: 1.38 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.314TrpAla: 1.314 ± 0.038	0.16TrpCys: 0.16 ± 0.012	0.609TrpAsp: 0.609 ± 0.025	0.563TrpGlu: 0.563 ± 0.023	0.568TrpPhe: 0.568 ± 0.025	0.955TrpGly: 0.955 ± 0.031	0.381TrpHis: 0.381 ± 0.021	0.599TrpIle: 0.599 ± 0.026	0.417TrpLys: 0.417 ± 0.018	1.765TrpLeu: 1.765 ± 0.045	0.356TrpMet: 0.356 ± 0.018	0.447TrpAsn: 0.447 ± 0.018	0.73TrpPro: 0.73 ± 0.025	0.575TrpGln: 0.575 ± 0.021	1.133TrpArg: 1.133 ± 0.033	0.753TrpSer: 0.753 ± 0.028	0.657TrpThr: 0.657 ± 0.026	0.916TrpVal: 0.916 ± 0.032	0.213TrpTrp: 0.213 ± 0.014	0.301TrpTyr: 0.301 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.66TyrAla: 2.66 ± 0.052	0.262TyrCys: 0.262 ± 0.015	1.303TyrAsp: 1.303 ± 0.032	1.242TyrGlu: 1.242 ± 0.036	0.896TyrPhe: 0.896 ± 0.031	2.063TyrGly: 2.063 ± 0.047	0.57TyrHis: 0.57 ± 0.023	1.043TyrIle: 1.043 ± 0.033	0.737TyrLys: 0.737 ± 0.029	2.366TyrLeu: 2.366 ± 0.049	0.461TyrMet: 0.461 ± 0.02	0.685TyrAsn: 0.685 ± 0.027	1.169TyrPro: 1.169 ± 0.034	0.87TyrGln: 0.87 ± 0.031	1.553TyrArg: 1.553 ± 0.04	1.142TyrSer: 1.142 ± 0.034	1.091TyrThr: 1.091 ± 0.037	1.563TyrVal: 1.563 ± 0.038	0.336TyrTrp: 0.336 ± 0.017	0.627TyrTyr: 0.627 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3751 proteins (1126875 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---