Lysinibacillus contaminans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lysinibacillus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3656 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M9X058|A0A0M9X058_9BACI Precorrin-2 dehydrogenase OS=Lysinibacillus contaminans OX=1293441 GN=AEA09_06460 PE=4 SV=1
MM1 pKa = 6.91SQEE4 pKa = 4.31HH5 pKa = 5.15NHH7 pKa = 6.21EE8 pKa = 4.35EE9 pKa = 4.15DD10 pKa = 3.39LHH12 pKa = 5.94VQHH15 pKa = 6.55ITVIDD20 pKa = 4.1DD21 pKa = 3.6NGNEE25 pKa = 3.96QLCEE29 pKa = 4.06VIHH32 pKa = 5.08VHH34 pKa = 6.48EE35 pKa = 4.83SPEE38 pKa = 4.03FGKK41 pKa = 10.83SYY43 pKa = 10.95VFYY46 pKa = 10.57SLVGAEE52 pKa = 4.0EE53 pKa = 4.64DD54 pKa = 3.74EE55 pKa = 4.53NGSVEE60 pKa = 3.97IFVSSFVPSEE70 pKa = 3.92NGEE73 pKa = 4.24DD74 pKa = 4.2GEE76 pKa = 4.32LTPIEE81 pKa = 4.84TEE83 pKa = 4.35AEE85 pKa = 3.47WDD87 pKa = 3.59MVEE90 pKa = 4.96DD91 pKa = 3.7VLNALEE97 pKa = 5.6DD98 pKa = 3.93EE99 pKa = 4.71DD100 pKa = 5.37EE101 pKa = 4.26EE102 pKa = 4.59

Molecular weight: 11.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M9X0Y1|A0A0M9X0Y1_9BACI Cytochrome C biogenesis protein OS=Lysinibacillus contaminans OX=1293441 GN=AEA09_12380 PE=4 SV=1
MM1 pKa = 6.99VPSRR5 pKa = 11.84STLVPSRR12 pKa = 11.84STSVPSRR19 pKa = 11.84STSVPSRR26 pKa = 11.84STSAPSRR33 pKa = 11.84STSAPSRR40 pKa = 11.84STSVPSRR47 pKa = 11.84STSAPSRR54 pKa = 11.84STSSSSRR61 pKa = 11.84STSTSSRR68 pKa = 11.84STTMSSRR75 pKa = 11.84STSAPSRR82 pKa = 11.84STSSS86 pKa = 2.79

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3655

1

3656

1067738

40

1976

292.1

32.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.603 ± 0.04

0.715 ± 0.012

4.976 ± 0.031

7.315 ± 0.052

4.636 ± 0.031

6.624 ± 0.036

2.014 ± 0.021

8.02 ± 0.041

6.494 ± 0.032

9.865 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.02

4.367 ± 0.029

3.384 ± 0.021

3.905 ± 0.027

3.748 ± 0.033

5.848 ± 0.029

5.796 ± 0.031

7.307 ± 0.032

0.96 ± 0.013

3.537 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski