Pteropus associated gemycircularvirus 5
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTN7|A0A140CTN7_9VIRU RepA OS=Pteropus associated gemycircularvirus 5 OX=1985399 PE=3 SV=1
MM1 pKa = 7.75 PSSFHH6 pKa = 6.1 LKK8 pKa = 8.75 NRR10 pKa = 11.84 RR11 pKa = 11.84 YY12 pKa = 10.59 VLFTYY17 pKa = 10.25 SQAGPDD23 pKa = 3.36 FDD25 pKa = 3.8 YY26 pKa = 10.74 WAVVDD31 pKa = 5.09 LLGDD35 pKa = 3.78 MGAEE39 pKa = 4.13 CIIGRR44 pKa = 11.84 EE45 pKa = 3.95 VHH47 pKa = 6.75 ADD49 pKa = 3.31 GGIHH53 pKa = 5.77 FHH55 pKa = 6.37 VFTDD59 pKa = 4.01 FGRR62 pKa = 11.84 LFSTRR67 pKa = 11.84 KK68 pKa = 9.62 VRR70 pKa = 11.84 VFDD73 pKa = 4.04 VGGKK77 pKa = 9.29 HH78 pKa = 6.54 PNIKK82 pKa = 10.26 PIGRR86 pKa = 11.84 TPAQAYY92 pKa = 9.94 DD93 pKa = 3.67 YY94 pKa = 10.4 AIKK97 pKa = 10.96 DD98 pKa = 3.52 GDD100 pKa = 4.04 VVAGGLGRR108 pKa = 11.84 PGGDD112 pKa = 4.46 CDD114 pKa = 3.84 WDD116 pKa = 3.87 PDD118 pKa = 4.19 NFWAAATHH126 pKa = 6.07 TGSSEE131 pKa = 4.05 EE132 pKa = 4.05 FLHH135 pKa = 7.11 FCDD138 pKa = 4.33 QLAPRR143 pKa = 11.84 DD144 pKa = 4.37 FIRR147 pKa = 11.84 GFTNFRR153 pKa = 11.84 AYY155 pKa = 11.08 SNWKK159 pKa = 8.82 FNPGIPEE166 pKa = 3.7 YY167 pKa = 10.13 DD168 pKa = 3.53 QPDD171 pKa = 3.75 GATYY175 pKa = 7.79 DD176 pKa = 3.43 TSAAPGIDD184 pKa = 3.03 EE185 pKa = 4.71 WLSQSLIGTGKK196 pKa = 9.78 SRR198 pKa = 11.84 EE199 pKa = 3.97 RR200 pKa = 3.54
Molecular weight: 22.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.248
IPC2_protein 5.219
IPC_protein 5.194
Toseland 5.397
ProMoST 5.474
Dawson 5.347
Bjellqvist 5.41
Wikipedia 5.321
Rodwell 5.27
Grimsley 5.448
Solomon 5.347
Lehninger 5.321
Nozaki 5.537
DTASelect 5.766
Thurlkill 5.537
EMBOSS 5.537
Sillero 5.614
Patrickios 3.948
IPC_peptide 5.359
IPC2_peptide 5.614
IPC2.peptide.svr19 5.616
Protein with the highest isoelectric point:
>tr|A0A140CTN6|A0A140CTN6_9VIRU Replication-associated protein OS=Pteropus associated gemycircularvirus 5 OX=1985399 PE=3 SV=1
MM1 pKa = 8.18 PYY3 pKa = 8.98 RR4 pKa = 11.84 TTRR7 pKa = 11.84 RR8 pKa = 11.84 GRR10 pKa = 11.84 RR11 pKa = 11.84 SFTRR15 pKa = 11.84 KK16 pKa = 7.23 VTPKK20 pKa = 10.36 RR21 pKa = 11.84 FGGTSRR27 pKa = 11.84 RR28 pKa = 11.84 TFPSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 FSGKK39 pKa = 9.47 RR40 pKa = 11.84 RR41 pKa = 11.84 FTSKK45 pKa = 10.16 RR46 pKa = 11.84 MTRR49 pKa = 11.84 KK50 pKa = 9.66 KK51 pKa = 10.79 VLDD54 pKa = 3.56 YY55 pKa = 10.7 TSKK58 pKa = 10.73 KK59 pKa = 9.58 KK60 pKa = 10.26 QDD62 pKa = 2.94 IMLQITNIDD71 pKa = 3.53 AGGVTPGPLRR81 pKa = 11.84 NGPSYY86 pKa = 10.96 LQANKK91 pKa = 10.03 TYY93 pKa = 10.14 LIPFAATARR102 pKa = 11.84 PAFGANLAPGNPADD116 pKa = 4.04 RR117 pKa = 11.84 AIRR120 pKa = 11.84 TSSEE124 pKa = 3.84 CYY126 pKa = 8.72 MRR128 pKa = 11.84 GIKK131 pKa = 9.74 EE132 pKa = 4.33 RR133 pKa = 11.84 ITIRR137 pKa = 11.84 TDD139 pKa = 4.11 SGAPWQWRR147 pKa = 11.84 RR148 pKa = 11.84 ICIKK152 pKa = 9.46 MRR154 pKa = 11.84 GSKK157 pKa = 10.37 FYY159 pKa = 10.55 DD160 pKa = 3.38 QEE162 pKa = 4.58 SGTDD166 pKa = 3.31 RR167 pKa = 11.84 MLSYY171 pKa = 10.92 LDD173 pKa = 4.13 NADD176 pKa = 3.77 IPQGMARR183 pKa = 11.84 VATTWTQSDD192 pKa = 4.0 SAWAVLCEE200 pKa = 3.99 EE201 pKa = 4.83 LFAGTRR207 pKa = 11.84 GYY209 pKa = 10.9 DD210 pKa = 2.97 WSDD213 pKa = 3.17 EE214 pKa = 3.95 MLAPLDD220 pKa = 3.76 KK221 pKa = 11.15 NRR223 pKa = 11.84 VSVAYY228 pKa = 10.07 DD229 pKa = 3.21 YY230 pKa = 7.48 TTTIRR235 pKa = 11.84 SGNDD239 pKa = 2.67 SGVYY243 pKa = 8.07 RR244 pKa = 11.84 TVNRR248 pKa = 11.84 WHH250 pKa = 7.12 PMNKK254 pKa = 8.14 TLYY257 pKa = 10.83 YY258 pKa = 10.54 DD259 pKa = 4.44 DD260 pKa = 3.86 QDD262 pKa = 4.04 KK263 pKa = 11.33 GDD265 pKa = 4.06 RR266 pKa = 11.84 LDD268 pKa = 3.35 SRR270 pKa = 11.84 RR271 pKa = 11.84 WSMEE275 pKa = 3.24 GNRR278 pKa = 11.84 GMGDD282 pKa = 3.24 YY283 pKa = 11.05 YY284 pKa = 10.42 IFDD287 pKa = 5.18 FIKK290 pKa = 11.09 GNGSPDD296 pKa = 3.38 DD297 pKa = 3.77 QLAIEE302 pKa = 4.54 YY303 pKa = 10.75 NSTLYY308 pKa = 9.56 WHH310 pKa = 7.05 EE311 pKa = 4.08 KK312 pKa = 9.32
Molecular weight: 36.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.274
IPC2_protein 9.516
IPC_protein 9.926
Toseland 10.058
ProMoST 9.867
Dawson 10.292
Bjellqvist 10.014
Wikipedia 10.511
Rodwell 10.54
Grimsley 10.394
Solomon 10.335
Lehninger 10.277
Nozaki 10.058
DTASelect 10.014
Thurlkill 10.131
EMBOSS 10.467
Sillero 10.204
Patrickios 9.765
IPC_peptide 10.321
IPC2_peptide 8.843
IPC2.peptide.svr19 8.615
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
834
200
322
278.0
31.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.554 ± 0.531
1.559 ± 0.313
8.753 ± 0.528
3.597 ± 0.378
5.755 ± 1.026
9.592 ± 0.793
2.758 ± 0.988
5.036 ± 0.122
4.556 ± 0.564
5.635 ± 0.412
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.918 ± 0.734
3.237 ± 0.441
5.156 ± 0.275
2.398 ± 0.265
8.393 ± 1.584
5.995 ± 0.641
6.235 ± 1.108
4.197 ± 0.599
3.237 ± 0.516
4.436 ± 0.467
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here